LOCUS       pMOD_D5802              5343 bp ds-DNA     circular     15-SEP-2021
DEFINITION  .
COMMENT     
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_feature    1122..1977
                     /locus_tag="High Copy Ori"
                     /ApEinfo_label="High Copy Ori"
                     /ApEinfo_fwdcolor="#800040"
                     /ApEinfo_revcolor="#800040"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1977..1983
                     /locus_tag="AarI"
                     /ApEinfo_label="AarI"
                     /ApEinfo_fwdcolor="#cc66ff"
                     /ApEinfo_revcolor="#cc66ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1988..1991
                     /locus_tag="AarI overhang"
                     /ApEinfo_label="AarI overhang"
                     /ApEinfo_fwdcolor="#000080"
                     /ApEinfo_revcolor="#000080"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     promoter        2004..3330
                     /locus_tag="AtUbi10 promoter"
                     /ApEinfo_label="AtUbi10 promoter"
                     /ApEinfo_fwdcolor="#d6b295"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3339..3341
                     /locus_tag="start"
                     /ApEinfo_label="start"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    4989..4991
                     /locus_tag="stop"
                     /ApEinfo_label="stop"
                     /ApEinfo_fwdcolor="#ff0000"
                     /ApEinfo_revcolor="#ff0000"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    5314..5317
                     /locus_tag="AarI overhang(1)"
                     /ApEinfo_label="AarI overhang"
                     /ApEinfo_fwdcolor="#000080"
                     /ApEinfo_revcolor="#000080"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             1..1054
                     /locus_tag="Amp R."
                     /ApEinfo_label="Amp R."
                     /ApEinfo_fwdcolor="#85dae9"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(5322..5328)
                     /locus_tag="AarI(1)"
                     /ApEinfo_label="AarI"
                     /ApEinfo_fwdcolor="#cc66ff"
                     /ApEinfo_revcolor="#cc66ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3339..4991
                     /locus_tag="Firefly Luciferase (Luc+)"
                     /ApEinfo_label="Firefly Luciferase (Luc+)"
                     /ApEinfo_fwdcolor="#0000fe"
                     /ApEinfo_revcolor="#0000fe"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
ORIGIN
        1 gtcaggtggc acttttcggg gaaatgtgcg atgaacccct atttgtttat ttttctaaat
       61 acattcaaat atgtatccgc taatgagaca ataaccctga taaatgcttc aattatattg
      121 aaaaaggaag agtatgagta tccaacattt ccgtgtcgcc cttattccct tttttgcggc
      181 cttctgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
      241 tcagttgggt gcccgagtgg gttacattga actggatctc aacagcggca aaatcttaga
      301 gagttttcgc cccgaagaac gcttcccaat gatgagcact ttcaaagttc tgctatgtgg
      361 cgcggtgtta tcccgtattg atgctgggca agagcaactc ggtcgccgca tacactattc
      421 tcagaatgac ttggttgagt attcaccagt cacagaaaag catcttacgg atggcatgac
      481 agtaagagaa ttatgtagtg ctgccataac catgagtgat aacactgcgg ccaacttact
      541 tctgacaacg attggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
      601 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
      661 tgacaccacg atgcctgtag caatggcaac aactctccgc aaactattaa ctggcgaact
      721 acttactctt gcttcacgcc agcaactcat tgactggatg gaggcggaca aagttgcagg
      781 accacttctg cgctcggcac ttccggctgg ctggtttatt gctgataaat ctggagccgg
      841 tgagcgtggC tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
      901 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
      961 tgagataggt gcctcactga ttaagcattg gtaactgtca gacgaaggga aataaatagt
     1021 agcccgcctg atgtgcgggc ttttttttgg tggtggttac cagtgtcgct tagctaccgt
     1081 aggtcctctt aggcggtccg attagcgaca tagtcatcaa tgccttgacg gcaaaatccc
     1141 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     1201 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     1261 agcggtggtt tgtttgccgg atcaagagct actaactctt tttccgaagg taactggctt
     1321 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
     1381 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     1441 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     1501 ggcgcagcgg tcgggctgaa cggggggttc gtgctcacag cccagcttgg agcgaacgac
     1561 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     1621 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     1681 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     1741 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     1801 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
     1861 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
     1921 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccacc
     1981 tgcaacgTCC CggcgcgccC TATgtcgagc tgcaggtcaa cggatcagga tattcttgtt
     2041 taagatgttg aactctatgg aggtttgtat gaactgatga tctaggaccg gataagttcc
     2101 cttcttcata gcgaacttat tcaaagaatg ttttgtgtat cattcttgtt acattgttat
     2161 taatgaaaaa atattattgg tcattggact gaacacgagt gttaaatatg gaccaggccc
     2221 caaataagat ccattgatat atgaattaaa taacaagaat aaatcgagtc accaaaccac
     2281 ttgccttttt taacgagact tgttcaccaa cttgatacaa aagtcattat cctatgcaaa
     2341 tcaataatca tacaaaaata tccaataaca ctaaaaaatt aaaagaaatg gataatttca
     2401 caatatgtta tacgataaag aagttacttt tccaagaaat tcactgattt tataagccca
     2461 cttgcattag ataaatggca aaaaaaaaca aaaaggaaaa gaaataaagc acgaagaatt
     2521 ctagaaaata cgaaatacgc ttcaatgcag tgggacccac ggttcaatta ttgccaattt
     2581 tcagctccac cgtatattta aaaaataaaa cgataatgct aaaaaaatat aaatcgtaac
     2641 gatcgttaaa tctcaacggc tggatcttat gacgaccgtt agaaattgtg gttgtcgacg
     2701 agtcagtaat aaacggcgtc aaagtggttg cagccggcac acacgagtcg tgtttatcaa
     2761 ctcaaagcac aaatactttt cctcaaccta aaaataaggc aattagccaa aaacaacttt
     2821 gcgtgtaaac aacgctcaat acacgtgtca ttttattatt agctattgct tcaccgcctt
     2881 agctttctcg tgacctagtc gtcctcgtct tttcttcttc ttcttctata aaacaatacc
     2941 caaagagctc ttcttcttca caattcagat ttcaatttct caaaatctta aaaactttct
     3001 ctcaattctc tctaccgtga tcaaggtaaa tttctgtgtt ccttattctc tcaaaatctt
     3061 cgattttgtt ttcgttcgat cccaatttcg tatatgttct ttggtttaga ttctgttaat
     3121 cttagatcga acacgatttt ctgggtttga tcgttagata tcatcttaat tctcgattag
     3181 ggtttcatag atatcatccg atttgttcaa ataatttgag ttttgtcgaa taattactct
     3241 tcgatttgtg atttctatct agatctggtg ttagtttcta gtttgtgcga tcgaatttgt
     3301 cgattaatct gagtttttct gattaacagg cctgcaggat ggaagacgcc aaaaacataa
     3361 agaaaggccc ggcgccattc tatccgctgg aagatggaac cgctggagag caactgcata
     3421 aggctatgaa gagatacgcc ctggttcctg gaacaattgc ttttacagat gcacatatcg
     3481 aggtggacat cacttacgct gagtacttcg aaatgtccgt tcggttggca gaagctatga
     3541 aacgatatgg gctgaataca aatcacagaa tcgtcgtatg cagtgaaaac tctcttcaat
     3601 tctttatgcc ggtgttgggc gcgttattta tcggagttgc agttgcgccc gcgaacgaca
     3661 tttataatga acgtgaattg ctcaacagta tgggcatttc gcagcctacc gtggtgttcg
     3721 tttccaaaaa ggggttgcaa aaaattttga acgtgcaaaa aaagctccca atcatccaaa
     3781 aaattattat catggattct aaaacggatt accagggatt tcagtcgatg tacacgttcg
     3841 tcacatctca tctacctccc ggttttaatg aatacgattt tgtgccagag tccttcgata
     3901 gggacaagac aattgcactg atcatgaact cctctggatc tactggtctg cctaaaggtg
     3961 tcgctctgcc tcatagaact gcctgcgtga gattctcgca tgccagagat cctatttttg
     4021 gcaatcaaat cattccggat actgcgattt taagtgttgt tccattccat cacggttttg
     4081 gaatgtttac tacactcgga tatttgatat gtggatttcg agtcgtctta atgtatagat
     4141 ttgaagaaga gctgtttctg aggagccttc aggattacaa gattcaaagt gcgctgctgg
     4201 tgccaaccct attctccttc ttcgccaaaa gcactctgat tgacaaatac gatttatcta
     4261 atttacacga aattgcttct ggtggcgctc ccctctctaa ggaagtcggg gaagcggttg
     4321 ccaagaggtt ccatctgcca ggtatcaggc aaggatatgg gctcactgag actacatcag
     4381 ctattctgat tacacccgag ggggatgata aaccgggcgc ggtcggtaaa gttgttccat
     4441 tttttgaagc gaaggttgtg gatctggata ccgggaaaac gctgggcgtt aatcaaagag
     4501 gcgaactgtg tgtgagaggt cctatgatta tgtccggtta tgtaaacaat ccggaagcga
     4561 ccaacgcctt gattgacaag gatggatggc tacattctgg agacatagct tactgggacg
     4621 aagacgaaca cttcttcatc gttgaccgcc tgaagtctct gattaagtac aaaggctatc
     4681 aggtggctcc cgctgaattg gaatccatct tgctccaaca ccccaacatc ttcgacgcTg
     4741 gtgtcgcagg tcttcccgac gatgacgccg gtgaacttcc cgccgccgtt gttgttttgg
     4801 agcacggaaa gacgatgacg gaaaaagaga tcgtggatta cgtcgccagt caagtaacaa
     4861 ccgcgaaaaa gttgcgcgga ggagttgtgt ttgtggacga agtaccgaaa ggtcttaccg
     4921 gaaaactcga cgcaagaaaa atcagagaga tcctcataaa ggccaagaag ggcggaaaga
     4981 tcgccgtgtg acTCGAggtt cgagtattat ggcattggga aaactgtttt tcttgtacca
     5041 tttgttgtgc ttgtaattta ctgtgttttt tattcggttt tcgctatcga actgtgaaat
     5101 ggaaatggat ggagaagagt taatgaatga tatggtcctt ttgttcattc tcaaattaat
     5161 attatttgtt ttttctctta tttgttgtgt gttgaatttg aaattataag agatatgcaa
     5221 acattttgtt ttgagtaaaa atgtgtcaaa tcgtggcctc taatgaccga agttaatatg
     5281 agGAGTAAAA CACTTGTAGT TGTGTTAgag ctcAGTGact agcaggtgat gaaaagcctt
     5341 agg     
//
