LOCUS       pMC_9_35S_11            2468 bp ds-DNA     circular     15-SEP-2021
DEFINITION  .
COMMENT     pCO798 from 1 to 2114
COMMENT     pJC222_J9-CcdB_J11 from 1 to 2892
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     rep_origin      1699..2381
                     /locus_tag="ColE1 origin"
                     /ApEinfo_label="ColE1 origin"
                     /ApEinfo_fwdcolor="gray50"
                     /ApEinfo_revcolor="gray50"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             594..1604
                     /locus_tag="Sm/Sp\no\DraIII"
                     /ApEinfo_label="Sm/Sp\no\DraIII"
                     /ApEinfo_fwdcolor="#66fffe"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1764..2378
                     /locus_tag="rep\(pMB1)"
                     /ApEinfo_label="rep\(pMB1)"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    136..141
                     /locus_tag="BsaI Binding site"
                     /ApEinfo_label="BsaI Binding site"
                     /ApEinfo_fwdcolor="#cccccc"
                     /ApEinfo_revcolor="#333333"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(369..374)
                     /locus_tag="BsaI Binding site(1)"
                     /ApEinfo_label="BsaI Binding site"
                     /ApEinfo_fwdcolor="#cccccc"
                     /ApEinfo_revcolor="#333333"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    147..357
                     /locus_tag="35S terminator"
                     /ApEinfo_label="35S terminator"
                     /ApEinfo_fwdcolor="#fc6fce"
                     /ApEinfo_revcolor="#fc6fce"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
ORIGIN
        1 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
       61 agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt
      121 cattaatcac tctgtggtct caGCTTgtcc gcaaaaatca ccagtctctc tctacaaatc
      181 tatctctctc tatttttctc cagaataatg tgtgagtagt tcccagataa gggaattagg
      241 gttcttatag ggtttcgctc atgtgttgag catataagaa acccttagta tgtatttgta
      301 tttgtaaaat acttctatca ataaaatttc taattcctaa aaccaaaatc cagtgacGAA
      361 TTCCGCTtga gaccacgaag tggctcttca gtggacgaaa gggcctcgtg atacgcctat
      421 ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg
      481 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
      541 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgcgct
      601 cacgcaactg gtccagaacc ttgaccgaac gcagcggtgg taacggcgca gtggcggttt
      661 tcatggcttg ttatgactgt ttttttgggg tacagtctat gcctcgggca tccaagcagc
      721 aagcgcgtta cgccgtgggt cgatgtttga tgttatggag cagcaacgat gttacgcagc
      781 agggcagtcg ccctaaaaca aagttaaaca tcatgaggga agcggtgatc gccgaagtat
      841 cgactcaact atcagaggta gttggcgtca tcgagcgcca tctcgaaccg acgttgctgg
      901 ccgtacattt gtacggctcc gcagtggatg gcggcctgaa gccacacagc gatattgatt
      961 tgctggttac ggtgaccgta aggcttgatg aaacaacgcg gcgagctttg atcaacgacc
     1021 ttttggaaac ttcggcttcc cctggagaga gcgagattct ccgcgctgta gaagtcacca
     1081 ttgttgtgca cgacgacatc attccgtggc gttatccagc taagcgcgaa ctgcaatttg
     1141 gagaatggca gcgcaatgac attcttgcag gtatcttcga gccagccacg atcgacattg
     1201 atctggctat cttgctgaca aaagcaagag aacatagcgt tgccttggta ggtccagcgg
     1261 cggaggaact ctttgatccg gttcctgaac aggatctatt tgaggcgcta aatgaaacct
     1321 taacgctatg gaactcgccg cccgactggg ctggcgatga gcgaaatgta gtgcttacgt
     1381 tgtcccgcat ttggtacagc gcagtaaccg gcaaaatcgc gccgaaggat gtcgctgccg
     1441 actgggcaat ggagcgcctg ccggcccagt atcagcccgt catacttgaa gctagacagg
     1501 cttatcttgg acaagaagaa gatcgcttgg cctcgcgcgc agatcagttg gaagaatttg
     1561 tccattacgt aaaaggcgag atcaccaagg tagtcggcaa ataactgtca gaccaagttt
     1621 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
     1681 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     1741 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     1801 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     1861 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     1921 tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     1981 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     2041 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     2101 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     2161 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     2221 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     2281 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     2341 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     2401 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
     2461 gtattacc     
//
