LOCUS       pMC_9_HSP_11            2501 bp ds-DNA     circular     15-SEP-2021
DEFINITION  Concatenation of 2 sequences.
ACCESSION   urn.local...a9o8s1c-4b
VERSION     urn.local...a9o8s1c-4b
KEYWORDS    .
SOURCE      
  ORGANISM   .
COMMENT     
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     CDS             711..1502
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy,
                     1985)"
                     /note="confers resistance to spectinomycin and
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQP VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
                     /locus_tag="SpR"
                     /ApEinfo_label="SpR"
                     /ApEinfo_fwdcolor="#e9d024"
                     /ApEinfo_revcolor="#e9d024"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     terminator      12..261
                     /Mismatches=0
                     /%_Identity=100
                     /Motif="ATATGAAGATGAAGATGAAATATTTGGTGTGTCAAATAAAAAGCTTGTGT
                     GCTTAAGTTTGTGTTTTTTTCTTGGCTTGTTGTGTTATGAATTTGTGGCTTTTTCTAA
                     TATTAAATGAATGTAAGATCTCATTATAATGAATAAACAAATGTTTCTATAATCCATT
                     GTGAATGTTTTGTTGGATCTCTTCTGCAGCATATAACTACTGTATGTGCTATGGTATG
                     GACTATGGAATATGATTAAAGATAAG"
                     /annotation_group="ATATGAAGATGAAGATGAAATATTTGGTGTGTCAAATAA
                     AAAGCTTGTGTGCTTAAGTTTGTGTTTTTTTCTTGGCTTGTTGTGTTATGAATTTGTG
                     GCTTTTTCTAATATTAAATGAATGTAAGATCTCATTATAATGAATAAACAAATGTTTC
                     TATAATCCATTGTGAATGTTTTGTTGGATCTCTTCTGCAGCATATAACTACTGTATGT
                     GCTATGGTATGGACTATGGAATATGATTAAAGATAAG: 9,412 -> 9,661"
                     /modified_by="User"
                     /X-Transferred_From="pMOD_A0101; pMOD_A0103; pMOD_A0111;
                     pMOD_A0201; pMOD_A0202; pMOD_A0208; pMOD_A0301;
                     pMOD_A0302; pMOD_A0308; pMOD_A0309 - BAD!!!; pMOD_A0401;
                     pMOD_A0402; pMOD_A0408; pMOD_A0409 - BAD!!!; pMOD_A0501;
                     pMOD_A0502; pMOD_A0503; pMOD_A0508; pMOD_A0509 - BAD!!!;
                     pMOD_A0601; pMOD_A0602; pMOD_A0608; pMOD_A0609 - BAD!!!;
                     pMOD_A0701; pMOD_A0702; pMOD_A0708; pMOD_A0709 - BAD!!!;
                     pMOD_A0801; pMOD_A0802; pMOD_A0808; pMOD_A0809 - BAD!!!;
                     pMOD_A0901; pMOD_A0902; pMOD_A0908 - BAD!!!; pMOD_A1110;
                     pMOD_A1210; pMOD_A1310; pMOD_A1410; pMOD_A1510;
                     pMOD_A1610; pMOD_A1710; pMOD_A1810; pMOD_A1910;
                     pMOD_A3001; pMOD_A3003; pMOD_A3010; pMOD_A3012;
                     pMOD_A3013; pMOD_A3014"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="pMOD_A0101, pMOD_A0103, pMOD_A0111,
                     pMOD_A0201 , pMOD_A0202, pMOD_A0208, pMOD_A0301,
                     pMOD_A0302, pMOD_A0308, pMOD_A0309 - BAD!!!, pMOD_A0401,
                     pMOD_A0402, pMOD_A0408, pMOD_A0409 - BAD!!!, pMOD_A0501,
                     pMOD_A0502, pMOD_A0503, pMOD_A0508, pMOD_A0509 - BAD!!!,
                     pMOD_A0601, pMOD_A0602, pMOD_A0608, pMOD_A0609 - BAD!!!,
                     pMOD_A0701, pMOD_A0702, pMOD_A0708, pMOD_A0709 - BAD!!!,
                     pMOD_A0801, pMOD_A0802, pMOD_A0808, pMOD_A0809 - BAD!!!,
                     pMOD_A0901, pMOD_A0902, pMOD_A0908 - BAD!!!, pMOD_A0909 -
                     BAD!!!, pMOD_A1110, pMOD_A1210, pMOD_A1310, pMOD_A1410,
                     pMOD_A1510, pMOD_A1610, pMOD_A1710, pMOD_A1810,
                     pMOD_A1910, pMOD_A3001, pMOD_A3003, pMOD_A3010,
                     pMOD_A3012, pMOD_A3013, pMOD_A3014"
                     /Transferred_Similarity="100.00%"
                     /locus_tag="HSP terminator"
                     /ApEinfo_label="HSP terminator"
                     /ApEinfo_fwdcolor="#9d1b1c"
                     /ApEinfo_revcolor="#9d1b1c"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1..6
                     /locus_tag="BsaI binding Site"
                     /ApEinfo_label="BsaI binding Site"
                     /ApEinfo_fwdcolor="#808080"
                     /ApEinfo_revcolor="#e6e6e6"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(267..272)
                     /locus_tag="BsaI binding Site(1)"
                     /ApEinfo_label="BsaI binding Site"
                     /ApEinfo_fwdcolor="#808080"
                     /ApEinfo_revcolor="#e6e6e6"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     rep_origin      1597..2279
                     /locus_tag="ColE1 origin"
                     /ApEinfo_label="ColE1 origin"
                     /ApEinfo_fwdcolor="gray50"
                     /ApEinfo_revcolor="gray50"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    8..11
                     /locus_tag="J9"
                     /ApEinfo_label="J9"
                     /ApEinfo_fwdcolor="#cc66ff"
                     /ApEinfo_revcolor="#cc66ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    262..265
                     /locus_tag="J11"
                     /ApEinfo_label="J11"
                     /ApEinfo_fwdcolor="#cc66ff"
                     /ApEinfo_revcolor="#cc66ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
ORIGIN
        1 ggtctcaGCT Tatatgaaga tgaagatgaa atatttggtg tgtcaaataa aaagcttgtg
       61 tgcttaagtt tgtgtttttt tcttggcttg ttgtgttatg aatttgtggc tttttctaat
      121 attaaatgaa tgtaagatca cattataatg aataaacaaa tgtttctata atccattgtg
      181 aatgttttgt tggatctctt ctgcagcata taactactgt atgtgctatg gtatggacta
      241 tggaatatga ttaaagataa gCGCTtgaga ccacgaagtg gctcttcagt ggacgaaagg
      301 gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt
      361 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
      421 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
      481 aaaggaagag tatgcgctca cgcaactggt ccagaacctt gaccgaacgc agcggtggta
      541 acggcgcagt ggcggttttc atggcttgtt atgactgttt ttttggggta cagtctatgc
      601 ctcgggcatc caagcagcaa gcgcgttacg ccgtgggtcg atgtttgatg ttatggagca
      661 gcaacgatgt tacgcagcag ggcagtcgcc ctaaaacaaa gttaaacatc atgagggaag
      721 cggtgatcgc cgaagtatcg actcaactat cagaggtagt tggcgtcatc gagcgccatc
      781 tcgaaccgac gttgctggcc gtacatttgt acggctccgc agtggatggc ggcctgaagc
      841 cacacagcga tattgatttg ctggttacgg tgaccgtaag gcttgatgaa acaacgcggc
      901 gagctttgat caacgacctt ttggaaactt cggcttcccc tggagagagc gagattctcc
      961 gcgctgtaga agtcaccatt gttgtgcacg acgacatcat tccgtggcgt tatccagcta
     1021 agcgcgaact gcaatttgga gaatggcagc gcaatgacat tcttgcaggt atcttcgagc
     1081 cagccacgat cgacattgat ctggctatct tgctgacaaa agcaagagaa catagcgttg
     1141 ccttggtagg tccagcggcg gaggaactct ttgatccggt tcctgaacag gatctatttg
     1201 aggcgctaaa tgaaacctta acgctatgga actcgccgcc cgactgggct ggcgatgagc
     1261 gaaatgtagt gcttacgttg tcccgcattt ggtacagcgc agtaaccggc aaaatcgcgc
     1321 cgaaggatgt cgctgccgac tgggcaatgg agcgcctgcc ggcccagtat cagcccgtca
     1381 tacttgaagc tagacaggct tatcttggac aagaagaaga tcgcttggcc tcgcgcgcag
     1441 atcagttgga agaatttgtc cattacgtaa aaggcgagat caccaaggta gtcggcaaat
     1501 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
     1561 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
     1621 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     1681 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     1741 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     1801 cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact
     1861 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     1921 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     1981 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     2041 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
     2101 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     2161 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     2221 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
     2281 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
     2341 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
     2401 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac
     2461 cgcctctccc cgcgcgttgg ccgattcatt aatcactctg t 
//
