LOCUS       pAAV-DIO-CAG-TVA        7004 bp ds-DNA     circular SYN 06-FEB-2017
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7004)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Sep 20, 2021 from SnapGene Viewer 5.2.4 to Vector 
            NTI(R) format
            https://www.snapgene.com
COMMENT     VNTDATE|779760000|
COMMENT     VNTDBDATE|796780800|
COMMENT     VNTNAME|pAAV-DIO-CAG-TVA-P2A-tomato|
FEATURES             Location/Qualifiers
     enhancer        296..599
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        601..878
                     /label=chicken beta-actin promoter
     protein_bind    1312..1345
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GGATACTT). lox2272 sites are compatible with each
                     other, but incompatible with loxP or loxN sites."
     protein_bind    1396..1429
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     CDS             complement(1447..2148)
                     /codon_start=1
                     /product="dimeric variant of DsRed fluorescent protein 
                     (Shaner et al., 2004)"
                     /label=dTomato
                     /note="mammalian codon-optimized"
     CDS             complement(2149..2205)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
     CDS             complement(2227..2664)
                     /codon_start=1
                     /label=TVA
     protein_bind    complement(2677..2710)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GGATACTT). lox2272 sites are compatible with each
                     other, but incompatible with loxP or loxN sites."
     protein_bind    complement(2761..2794)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     misc_feature    2819..3407
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(3290..3301)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
     polyA_signal    3440..3664
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     promoter        complement(3885..4214)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3899..4034
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     rep_origin      complement(4460..5048)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5219..6079)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
     promoter        complement(6080..6184)
                     /gene="bla"
                     /label=AmpR promoter
     promoter        6972..6990
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
ORIGIN
        1 ttctagctgc gcgctcgctc gctcactgag gccgcccggg caaagcccgg gcgtcgggcg
       61 acctttggtc gcccggcctc agtgagcgag cgagcgcgca gagagggagt ggccaactcc
      121 atcactaggg gttccttgta gttaatgatt aacccgccat gctacttatc tacgtagcca
      181 tgctctaggt acctaatatc acaaactgga aatgtctatc aatatatagt tgctctagtt
      241 attaatagta atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta
      301 cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt
      361 caataatgac gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg
      421 tggactattt acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta
      481 cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga
      541 ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg
      601 tcgaggtgag ccccacgttc tgcttcactc tccccatctc ccccccctcc ccacccccaa
      661 ttttgtattt atttattttt taattatttt gtgcagcgat gggggcgggg gggggggggg
      721 ggcgcgcgcc aggcggggcg gggcggggcg aggggcgggg cggggcgagg cggagaggtg
      781 cggcggcagc caatcagagc ggcgcgctcc gaaagtttcc ttttatggcg aggcggcggc
      841 ggcggcggcc ctataaaaag cgaagcgcgc ggcgggcggg agtcgctgcg acgctgcctt
      901 cgccccgtgc cccgctccgc cgccgcctcg cgccgcccgc cccggctctg actgaccgcg
      961 ttactcccac aggtgagcgg gcgggacggc ccttctcctc cgggctgtaa ttagcgcttg
     1021 gtttaatgac ggcttgtttc ttttctgtgg ctgcgtgaaa gccttgaggg gctccgggag
     1081 ggccctttgt gcggggggag cggctcgggg ctgtccgcgg ggggacggct gccttcgggg
     1141 gggacggggc agggcggggt tcggcttctg gcgtgtgacc ggcggctcta gagcctctgc
     1201 taaccatgtt catgccttct tctttttcct acagctcctg ggcaacgtgc tggttattgt
     1261 gctgtctcat cattttggca aagaattgga tcctctagag tcgactccgg aataacttcg
     1321 tataggatac tttatacgaa gttatgcaga atggtagctg gattgtagct gctattagca
     1381 atatgaaacc tcttaataac ttcgtatagc atacattata cgaagttatg gcgcgccgtc
     1441 gacttacttg tacagctcgt ccatgccgta caggaacagg tggtggcggc cctcggagcg
     1501 ctcgtactgt tccacgatgg tgtagtcctc gttgtgggag gtgatgtcca gcttggtgtc
     1561 cacgtagtag tagccgggca gttgcacggg cttcttggcc atgtagatgg tcttgaactc
     1621 caccaggtag tggccgccgt ccttcagctt cagggcctgg tggatctcgc ccttcagcac
     1681 gccgtcgcgg gggtacaggc gctcggtgga ggcctcccag cccatggtct tcttctgcat
     1741 tacggggccg tcggggggga agttggtgcc gcgcatcttc accttgtaga tcagcgtgcc
     1801 gtcctgcagg gaggagtcct gggtcacggt caccagaccg ccgtcctcga agttcatcac
     1861 gcgctcccac ttgaagccct cggggaagga cagcttcttg taatcgggga tgtcggcggg
     1921 gtgcttcacg tacgccttgg agccgtacat gaactggggg gacaggatgt cccaggcgaa
     1981 gggcaggggg ccgcccttgg tcaccttcag cttggcggtc tgggtgccct cgtaggggcg
     2041 gccctcgccc tcgccctcga tctcgaactc gtggccgttc atggagccct ccatgcgcac
     2101 cttgaagcgc atgaactctt tgatgacctc ctcgcccttg ctcaccattg gcccgggatt
     2161 ctcttcgaca tcccctgctt gtttcaacag ggagaagtta gtggctccgc ttccgttgtc
     2221 ttcgaaccca ccccccttgg atgccctttc aagactgaaa atgtcaaacc tgctttttgc
     2281 tttggacttc ccccatgcag cgataccccc cacagctacc aggcagcaca ggggccctgc
     2341 agtgatcagc atccacatgc ggccgtgatt cctggctggc aaagcacgtc cagaagcagg
     2401 ggcagtggga gcctctgtgc cgttgtccgt gggcaccgcg gggatcgcgc tggtcccgca
     2461 cccccactcg tcccgcccgt cgtcgcagtc ggggtgtccg tcgcacagcc agtcctgcgg
     2521 gtaacactcc ccgtgggcac cgggcggctc cgagcagcgg aactgaccgg gggggcaacg
     2581 ggacaaaaaa ccgttaccgg acccgttacc ggtcacgtta ccgggcagca gcagcagcag
     2641 cagcgcgggc agcagccgcg ccatggtggc gctagcataa cttcgtataa agtatcctat
     2701 acgaagttat ttgccttaac ccagaaatta tcactgttat tctttagaat ggtgcaaaga
     2761 ataacttcgt ataatgtatg ctatacgaag ttatgaattc gatatcaagc ttatcgataa
     2821 tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc
     2881 ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat
     2941 ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg
     3001 gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg
     3061 ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat
     3121 tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt
     3181 gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc
     3241 ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa
     3301 tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg
     3361 ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcatc gataccgtcg
     3421 actcgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc
     3481 cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag
     3541 gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag
     3601 gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct
     3661 atggcttctg aggcggaaag aaccagctgg ggctcgacta gagcatggct acgtagataa
     3721 gtagcatggc gggttaatca ttaactacaa ggaaccccta gtgatggagt tggccactcc
     3781 ctctctgcgc gctcgctcgc tcactgaggc cgggcgacca aaggtcgccc gacgcccggg
     3841 ctttgcccgg gcggcctcag tgagcgagcg agcgcgcaga gctttttgca aaagcctagg
     3901 cctccaaaaa agcctcctca ctacttctgg aatagctcag aggccgaggc ggcctcggcc
     3961 tctgcataaa taaaaaaaat tagtcagcca tggggcggag aatgggcgga actgggcgga
     4021 gttaggggcg ggatgggcgg agttaggggc gggactatgg ttgctgacta attgagatgc
     4081 atgctttgca tacttctgcc tgctggggag cctggggact ttccacacct ggttgctgac
     4141 taattgagat gcatgctttg catacttctg cctgctgggg agcctgggga ctttccacac
     4201 cctaactgac acacattcca cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
     4261 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
     4321 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     4381 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     4441 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     4501 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     4561 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     4621 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     4681 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     4741 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     4801 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     4861 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
     4921 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     4981 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     5041 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     5101 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     5161 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     5221 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     5281 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     5341 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
     5401 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     5461 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     5521 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     5581 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     5641 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     5701 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     5761 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
     5821 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
     5881 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
     5941 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
     6001 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
     6061 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
     6121 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
     6181 cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat
     6241 taacctataa aaataggcgt atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg
     6301 gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg
     6361 ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc
     6421 ttaactatgc ggcatcagag cagattgtac tgagagtgca ccattcgacg ctctccctta
     6481 tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc
     6541 gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc
     6601 accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca
     6661 tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc
     6721 acgatgcgtc cggcgtagag gatctggcta gcgatgaccc tgctgattgg ttcgctgacc
     6781 atttccgggt gcgggacggc gttaccagaa actcagaagg ttcgtccaac caaaccgact
     6841 ctgacggcag tttacgagag agatgatagg gtctgcttca gtaagccaga tgctacacaa
     6901 ttaggcttgt acatattgtc gttagaacgc ggctacaatt aatacataac cttatgtatc
     6961 atacacatac gatttaggtg acactataga atacacggaa ttaa
//
