LOCUS       pAAV-SEO-CaMKII-        6646 bp ds-DNA     circular SYN 20-JUN-2018
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 6646)
  AUTHORS   Yoav Ben Simon
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Sep 20, 2021 from SnapGene Viewer 5.2.4 to Vector 
            NTI(R) format
            https://www.snapgene.com
COMMENT     VNTDATE|844992000|
COMMENT     VNTDBDATE|844992000|
COMMENT     VNTNAME|pAAV-SEO-CaMKII-EGFP (Yoav)|
COMMENT     VNTAUTHORNAME|Yoav Ben Simon|
FEATURES             Location/Qualifiers
     LTR             5..136
                     /label=ITR
     protein_bind    216..249
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     promoter        284..1576
                     /label=CaMKII promoter
     CDS             1598..2317
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /note="mammalian codon-optimized"
     protein_bind    2372..2405
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     misc_feature    2461..3049
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(2932..2943)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
     polyA_signal    3082..3306
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     LTR             3392..3523
                     /label=ITR
     promoter        complement(3527..3856)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3541..3676
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     rep_origin      complement(4102..4690)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4861..5721)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
     promoter        complement(5722..5826)
                     /gene="bla"
                     /label=AmpR promoter
     promoter        6614..6632
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
ORIGIN
        1 ttctagctgc gcgctcgctc gctcactgag gccgcccggg caaagcccgg gcgtcgggcg
       61 acctttggtc gcccggcctc agtgagcgag cgagcgcgca gagagggagt ggccaactcc
      121 atcactaggg gttccttgta gttaatgatt aacccgccat gctacttatc tacgtagcca
      181 tgctctaggt accttccagc aggtcgaggg acctaataac ttcgtatagc atacattata
      241 cgaagttata ttaagggttc caagcttaag cggccgcacg cgtttaacat tatggcctta
      301 ggtcacttca tctccatggg gttcttcttc tgattttcta gaaaatgaga tgggggtgca
      361 gagagcttcc tcagtgacct gcccagggtc acatcagaaa tgtcagagct agaacttgaa
      421 ctcagattac taatcttaaa ttccatgcct tgggggcatg caagtacgat atacagaagg
      481 agtgaactca ttagggcaga tgaccaatga gtttaggaaa gaagagtcca gggcagggta
      541 catctacacc acccgcccag ccctgggtga gtccagccac gttcacctca ttatagttgc
      601 ctctctccag tcctaccttg acgggaagca caagcagaaa ctgggacagg agccccagga
      661 gaccaaatct tcatggtccc tctgggagga tgggtgggga gagctgtggc agaggcctca
      721 ggaggggccc tgctgctcag tggtgacaga taggggtgag aaagcagaca gagtcattcc
      781 gtcagcattc tgggtctgtt tggtacttct tctcacgcta aggtggcggt gtgatatgca
      841 caatggctaa aaagcaggga gagctggaaa gaaacaagga cagagacaga ggccaagtca
      901 accagaccaa ttcccagagg aagcaaagaa accattacag agactacaag ggggaaggga
      961 aggagagatg aattagcttc ccctgtaaac cttagaaccc agctgttgcc agggcaacgg
     1021 ggcaatacct gtctcttcag aggagatgaa gttgccaggg taactacatc ctgtctttct
     1081 caaggaccat cccagaatgt ggcacccact agccgttacc atagcaactg cctctttgcc
     1141 ccacttaatc ccatcccgtc tgttaaaagg gccctatagt tggaggtggg ggaggtagga
     1201 agagcgatga tcacttgtgg actaagtttg ttcgcatccc cttctccaac cccctcagta
     1261 catcaccctg ggggaacagg gtccacttgc tcctgggccc acacagtcct gcagtattgt
     1321 gtatataagg ccagggcaaa gaggagcagg ttttaaagtg aaaggcaggc aggtgttggg
     1381 gaggcagtta ccggggcaac gggaacaggg cgtttcggag gtggttgcca tggggacctg
     1441 gatgctgacg aaggctcgcg aggctgtgag cagccacagt gccctgctca gaagccccaa
     1501 gctcgtcagt caagccggtt ctccgtttgc actcaggagc acgggcaggc gagtggcccc
     1561 tagttctggg ggcagcgggg gatccactag cgccaccatg gtgagcaagg gcgaggagct
     1621 gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt
     1681 cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat
     1741 ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg
     1801 cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc
     1861 catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa
     1921 gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg
     1981 catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag
     2041 ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat
     2101 ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc
     2161 catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccgccct
     2221 gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc
     2281 cgggatcact ctcggcatgg acgagctgta caagtaaagc ggccgcgact ctagggatcc
     2341 ctagggcgcg ccgaattcgt cgagggacct aataacttcg tatagcatac attatacgaa
     2401 gttatacatg tttaagggtt ccggttccac taggtacaat tcgatatcaa gcttatcgat
     2461 aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct
     2521 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt
     2581 atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg
     2641 tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact
     2701 ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct
     2761 attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg
     2821 ttgggcactg acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc
     2881 gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc
     2941 aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt
     3001 cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgca tcgataccgt
     3061 cgactcgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct
     3121 cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg
     3181 aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc
     3241 aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct
     3301 ctatggcttc tgaggcggaa agaaccagct ggggctcgac tagagcatgg ctacgtagat
     3361 aagtagcatg gcgggttaat cattaactac aaggaacccc tagtgatgga gttggccact
     3421 ccctctctgc gcgctcgctc gctcactgag gccgggcgac caaaggtcgc ccgacgcccg
     3481 ggctttgccc gggcggcctc agtgagcgag cgagcgcgca gagctttttg caaaagccta
     3541 ggcctccaaa aaagcctcct cactacttct ggaatagctc agaggccgag gcggcctcgg
     3601 cctctgcata aataaaaaaa attagtcagc catggggcgg agaatgggcg gaactgggcg
     3661 gagttagggg cgggatgggc ggagttaggg gcgggactat ggttgctgac taattgagat
     3721 gcatgctttg catacttctg cctgctgggg agcctgggga ctttccacac ctggttgctg
     3781 actaattgag atgcatgctt tgcatacttc tgcctgctgg ggagcctggg gactttccac
     3841 accctaactg acacacattc cacagctgca ttaatgaatc ggccaacgcg cggggagagg
     3901 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
     3961 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     4021 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     4081 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     4141 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     4201 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     4261 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     4321 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     4381 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     4441 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     4501 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg
     4561 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     4621 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     4681 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     4741 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     4801 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
     4861 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
     4921 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
     4981 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
     5041 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
     5101 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
     5161 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
     5221 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
     5281 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
     5341 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
     5401 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
     5461 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
     5521 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
     5581 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
     5641 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
     5701 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
     5761 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
     5821 tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac
     5881 attaacctat aaaaataggc gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga
     5941 cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga
     6001 tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg
     6061 gcttaactat gcggcatcag agcagattgt actgagagtg caccattcga cgctctccct
     6121 tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg
     6181 ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg
     6241 ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc
     6301 catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg
     6361 ccacgatgcg tccggcgtag aggatctggc tagcgatgac cctgctgatt ggttcgctga
     6421 ccatttccgg gtgcgggacg gcgttaccag aaactcagaa ggttcgtcca accaaaccga
     6481 ctctgacggc agtttacgag agagatgata gggtctgctt cagtaagcca gatgctacac
     6541 aattaggctt gtacatattg tcgttagaac gcggctacaa ttaatacata accttatgta
     6601 tcatacacat acgatttagg tgacactata gaatacacgg aattaa
//
