LOCUS       pEPQD0CM0068_(L0_NmMLP) 2541 bp ds-DNA     circular     21-OCT-2020
DEFINITION  .
FEATURES             Location/Qualifiers
     primer          263..285
                     /label="pUAP/DP2-F"
                     /note="sequence: ggcgtatcacgaggcagaatttc"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     CDS             341..823
                     /label="Translation 341-823"
     misc_feature    344..820
                     /label="MLPL1 from Nepeta mussinii (aka Nepeta racemosa) MT108267.1, with ISY will make cis-trans-nepetalactol ("cis-trans"-cyclase)"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    821..823
                     /label="Stop codon"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     primer          complement(850..870)
                     /label="pUAP/DP2-R"
                     /note="sequence: ggtggtgacaccttgcccttt"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     primer          complement(997..1022)
                     /label="211or (L0 FWD)"
                     /note="sequence: CGTTATCCCCTGATTCTGTGGATAAC"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     Replication_ori complement(1093..1681)
                     /label="ori"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     primer          complement(1138..1156)
                     /label="QMD062_pJL1_outside(BR322)_F"
                     /note="sequence: tcgccacctctgacttgag"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer          complement(1140..1159)
                     /label="QMD132_LETprom2_F, 61.4"
                     /note="sequence: gtttcgccacctctgacttg"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer          complement(1169..1192)
                     /label="QMD131_LETprom1_F, 61.7, -3.89 self"
                     /note="sequence: gggaaacgcctggtatctttatag"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     Terminator      1864..1958
                     /label="lambda t0 terminator"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     CDS             complement(join(1979..2541,1..97))
                     /label="CmR"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
ORIGIN
        1 AGGTACATTG AGCAACTGAC TGAAATGCCT CAAAATGTTC TTTACGATGC CATTGGGATA
       61 TATCAACGGT GGTATATCCA GTGATTTTTT TCTCCATTTT AGCTTCCTTA GCTCCTGAAA
      121 ATCTCGATAA CTCAAAAAAT ACGCCCGGTA GTGATCTTAT TTCATTATGG TGAAAGTTGG
      181 AACCTCTTAC GTGCCCGATC AACTCGAGTG CCACCTGACG TCTAAGAAAC CATTATTATC
      241 ATGACATTAA CCTATAAAAA TAGGCGTATC ACGAGGCAGA ATTTCAGATA AAAAAAATCC
      301 TTAGCTTTCG CTAAGGATGA TTTCTGGAAT TCGGTCTCAA ATGGCTTCGA AGCTTGAAAT
      361 AGAAATTGAG TTGAAAAGTG ATGTAGAAAA GATGTGGAAA CACTTCAAAG AATTCACCAA
      421 ATTATTCCCC AAGGCTTGTC CTCATCTTTA CGAGAAGATT GATGTGATCG AGGGAGATGG
      481 GATATCAGTT GGAACAATCT TTGTCAGTAC CCTGAAACCA ACAGAGTTGA ACCCCGTGGT
      541 TATGGTGACG AAGGAGAAGA TTGATTTTTT AGATGATGAG AACAAAATGT TGAGGTATAG
      601 TTACATGGAA GGGGAGATAT TAAAGAATTA CAAGAATTTG AGGGGCACAG TGCATATGAG
      661 CAGCAGCAAA AGTGGTGGAA CTATATTCAA ATATTCAGGA GAATTCGAAA AGGCAAATGA
      721 ACAAGTCCCT GATCCTGTTT TCTTCAAAGA TTTTATGGTC ATAGTTTTCC AAGGTTTGGA
      781 TGATTATATT CTCAAGGGCA TGAACCATAC ATGTCAAAAT TAAGCTTCGA GACCCTGCAG
      841 TCCGGCAAAA AAGGGCAAGG TGTCACCACC CTGCCCTTTT TCTTTAAAAC CGAAAAGATT
      901 ACTTCGCGTT ATGCAGGCTT CCTCGCTCAC TGACTCGCTG CGCTCGGTCG TTCGGCTGCG
      961 GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA
     1021 CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC
     1081 GTTGCTGGCG TTTTTCCACA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC
     1141 AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG
     1201 CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT
     1261 CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA
     1321 GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC
     1381 CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC
     1441 AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT
     1501 GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT
     1561 GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC
     1621 TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA
     1681 AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA
     1741 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA
     1801 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GCTCGAGGCT
     1861 TGGATTCTCA CCAATAAAAA ACGCCCGGCG GCAACCGAGC GTTCTGAACA AATCCAGATG
     1921 GAGTTCTGAG GTCATTACTG GATCTATCAA CAGGAGTCCA AGCGAGCTCG ATATCAAATT
     1981 ACGCCCCGCC CTGCCACTCA TCGCAGTACT GTTGTAATTC ATTAAGCATT CTGCCGACAT
     2041 GGAAGCCATC ACAAACGGCA TGATGAACCT GAATCGCCAG CGGCATCAGC ACCTTGTCGC
     2101 CTTGCGTATA ATATTTGCCC ATGGTGAAAA CGGGGGCGAA GAAGTTGTCC ATATTGGCCA
     2161 CGTTTAAATC AAAACTGGTG AAACTCACCC AGGGATTGGC TGAGACGAAA AACATATTCT
     2221 CAATAAACCC TTTAGGGAAA TAGGCCAGGT TTTCACCGTA ACACGCCACA TCTTGCGAAT
     2281 ATATGTGTAG AAACTGCCGG AAATCGTCGT GGTATTCACT CCAGAGCGAT GAAAACGTTT
     2341 CAGTTTGCTC ATGGAAAACG GTGTAACAAG GGTGAACACT ATCCCATATC ACCAGCTCAC
     2401 CGTCTTTCAT TGCCATACGA AATTCCGGAT GAGCATTCAT CAGGCGGGCA AGAATGTGAA
     2461 TAAAGGCCGG ATAAAACTTG TGCTTATTTT TCTTTACGGT CTTTAAAAAG GCCGTAATAT
     2521 CCAGCTGAAC GGTCTGGTTA T
//