LOCUS       pEPQD0CM0061_(L0_CrIO), 3606 bp ds-DNA     circular     20-OCT-2018
DEFINITION  .
FEATURES             Location/Qualifiers
     CDS              68..1615
                      /label="Translation 68-1615"
     misc_feature     71..1615
                      /label="CrIO (contains T359S mutation, domesticated, 1 BpiI site and 1 BsmBI site removed) >ENA AHK60833 AHK60833.1 Catharanthus roseus (Madagascar periwinkle) iridoid oxidase "
                      /ApEinfo_revcolor=#f8d3a9
                      /ApEinfo_fwdcolor=#f8d3a9
     misc_feature     1142..1144
                      /label="ACC threo to AGC ser"
                      /ApEinfo_revcolor=#ff9ccd
                      /ApEinfo_fwdcolor=#ff9ccd
     misc_feature     1143..1143
                      /label="SNP here? Sequencing says G. YES it is a G"
                      /ApEinfo_revcolor=#85dae9
                      /ApEinfo_fwdcolor=#85dae9
     primer           complement(1162..1185)
                      /label="CrIOmid3_R binding region 60.2"
                      /note="sequence: TCCTTTACAACTGCTTGCAAATAC"
                      /ApEinfo_revcolor=#ffef86
                      /ApEinfo_fwdcolor=#ffef86
     primer           complement(1162..1197)
                      /label="QMD114_CrIOmid3_R"
                      /note="sequence: agaGAAGACtaTTCCTTTACAACTGCTTGCAAATAC"
                      /ApEinfo_revcolor=#b7e6d7
                      /ApEinfo_fwdcolor=#b7e6d7
     primer           1172..1205
                      /label="QMD113_CrIOmid3_F"
                      /note="sequence: agaGAAGACtaGGAAACGCTTAGATTGCATCCGG"
                      /ApEinfo_revcolor=#c6c9d1
                      /ApEinfo_fwdcolor=#c6c9d1
     misc_feature     1184..1186
                      /label="GAG glu to GAA glu to remove BsmBI"
                      /ApEinfo_revcolor=#d6b295
                      /ApEinfo_fwdcolor=#d6b295
     primer           1187..1205
                      /label="CrIOmid3_F binding region 61.6"
                      /note="sequence: ACGCTTAGATTGCATCCGG"
                      /ApEinfo_revcolor=#c7b0e3
                      /ApEinfo_fwdcolor=#c7b0e3
     misc_feature     1238..1240
                      /label="GAA glu to GAG glu"
                      /ApEinfo_revcolor=#ff9ccd
                      /ApEinfo_fwdcolor=#ff9ccd
     restriction site 1616..1621
                      /label="XhoI"
                      /ApEinfo_revcolor=#ffef86
                      /ApEinfo_fwdcolor=#ffef86
     Replication_ori  complement(1885..2473)
                      /label="ori"
                      /ApEinfo_revcolor=#faac61
                      /ApEinfo_fwdcolor=#faac61
     Terminator       2656..2750
                      /label="lambda t0 terminator"
                      /ApEinfo_revcolor=#9eafd2
                      /ApEinfo_fwdcolor=#9eafd2
     CDS              complement(2771..3430)
                      /label="CmR"
                      /ApEinfo_revcolor=#84b0dc
                      /ApEinfo_fwdcolor=#84b0dc
ORIGIN
        1 AGGCAGAATT TCAGATAAAA AAAATCCTTA GCTTTCGCTA AGGATGATTT CTGGAATTCG
       61 GTCTCAAATG GCGACCATCA CTTTCGATTC ACTAAACCCG GTGACCGTCG CCATCTCAGC
      121 CGGTTTTCTT CTCCTCCTTA TCATCTTTGT GAAATCAAGA ACTGGTTCAT CCAAAAGAAA
      181 ACCGCCGGGT CCTCCAGGAT GGCCGATTTT CGGCAATATG TTCGACCTCG GAGATTTACC
      241 TCACCAAACG CTGTACAAGC TCAAGTCCAA ATACGGGCCG ATCGTATGGC TCCAGCTCGG
      301 CTCAATTAAT ACTATGGTTG TTCAAAATGC AGTTTCCGCC GCCGAGCTTT TTAAGAAACA
      361 CGATGTCCCC TTTTGTGACC GAAAAGTCCC TGATACTCTC ACTGCCTTCA ATTTCAACCA
      421 GGGCTCCCTG GGTATGAATA CTTACGGCGG ACATTGGCGA GTGCTTAGAC GCCTCTGTTC
      481 CATGGAGTTT TTGGTAAATA AACGTATGAA CGAAACTACA GATCTAAGAA GGAGAATTGA
      541 GGACAATATG GTCCGTTGGA TTGAAGAAGA TTCATTGGCA TCTAAAGCAC AAGGAGGAAC
      601 CGGAGCGGTT CAATTGAGCA GGTTTCTTTT TCTAATGGCG TTTAATTTGG TGGGTAACCT
      661 TATGTTATCG AGAGATTTAA TGGATAATAA GGATCCAGAA GGAAGGGAAT TTTTTGATTG
      721 TATGAATGAG ATATTGGAAT TGGCTGGTAC TCCTAATATT GCTGATTTTT TGCCTTTGTT
      781 GAAGAAATTG GATCCACTTG GAATGAAGAA AAGGATGGTT GATAATATGT CTAGAACTAT
      841 GAAAATTTCT TCAAAGTTTG TTCAAGAAAG ACTTGATAAT AGAAAAGCTG GAAAGATTAA
      901 TGAAAAGAAA GATTTCTTGG ATGTTATGCT TGAATACCAA GGTGATGGTA AAGATGGTCC
      961 CGATAAATTC ACTGAGCAAC ATGTCAACAT TGTCATCATG GAAATGTTTT TTGCAGGATC
     1021 AGAGACAACA AGTATCAGCA TTGAATGGGG ATTCACAGAA CTTCTCCGAA ACCCACACGC
     1081 ATTCAAGAAA GTAAGGGAAG AGATCGACAG AGTAGTGGGA GTAAACAGAA TGGTCGAAGA
     1141 GAGCGACATG GAGAATTTGC CGTATTTGCA AGCAGTTGTA AAGGAAACGC TTAGATTGCA
     1201 TCCGGCACTT CCAATGCTTC TACCAAGAAA CACAATGGAG GACACTGAAT ACATGGGATA
     1261 CCTCATTCCT AAAGGCACAC AAGTTTTCGT GAATGCATGG GCAATCGGAA GGGATCCTGA
     1321 GTACTGGCAG GACCCTTTGA GTTTCAAACC AGAAAGGTTT ATCAATTCCA GTGTTGAATA
     1381 TAAAGGACAA CATTTTGAAT TGATTCCTTT TGGTTCTGGA AGAAGAATTT GTGTGGGATT
     1441 TCCTTTGGCT CATAGAGTTG TTCATCTCAC TTTGGCTACA TTAGTCCAGG CTTTTGATTG
     1501 GGATCTTGGT GCTGGCGTTA AACCACAAGA TATTGATTTG GAAGAGAGAT TGGGATTGAC
     1561 TCTGAGGAAG AAGAATCCAT TGAATGTCAT CCCTAAGAAG AGAGTTCATA TCTAAGCTTC
     1621 GAGACCCTGC AGTCCGGCAA AAAAGGGCAA GGTGTCACCA CCCTGCCCTT TTTCTTTAAA
     1681 ACCGAAAAGA TTACTTCGCG TTATGCAGGC TTCCTCGCTC ACTGACTCGC TGCGCTCGGT
     1741 CGTTCGGCTG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA
     1801 ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG
     1861 TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA CAGGCTCCGC CCCCCTGACG AGCATCACAA
     1921 AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT
     1981 TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT
     2041 GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT
     2101 CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC
     2161 CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT
     2221 ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC
     2281 TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT
     2341 CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA
     2401 ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA
     2461 AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA
     2521 AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT
     2581 TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAA CTTGGTCTGA
     2641 CAGCTCGAGG CTTGGATTCT CACCAATAAA AAACGCCCGG CGGCAACCGA GCGTTCTGAA
     2701 CAAATCCAGA TGGAGTTCTG AGGTCATTAC TGGATCTATC AACAGGAGTC CAAGCGAGCT
     2761 CGATATCAAA TTACGCCCCG CCCTGCCACT CATCGCAGTA CTGTTGTAAT TCATTAAGCA
     2821 TTCTGCCGAC ATGGAAGCCA TCACAAACGG CATGATGAAC CTGAATCGCC AGCGGCATCA
     2881 GCACCTTGTC GCCTTGCGTA TAATATTTGC CCATGGTGAA AACGGGGGCG AAGAAGTTGT
     2941 CCATATTGGC CACGTTTAAA TCAAAACTGG TGAAACTCAC CCAGGGATTG GCTGAGACGA
     3001 AAAACATATT CTCAATAAAC CCTTTAGGGA AATAGGCCAG GTTTTCACCG TAACACGCCA
     3061 CATCTTGCGA ATATATGTGT AGAAACTGCC GGAAATCGTC GTGGTATTCA CTCCAGAGCG
     3121 ATGAAAACGT TTCAGTTTGC TCATGGAAAA CGGTGTAACA AGGGTGAACA CTATCCCATA
     3181 TCACCAGCTC ACCGTCTTTC ATTGCCATAC GAAATTCCGG ATGAGCATTC ATCAGGCGGG
     3241 CAAGAATGTG AATAAAGGCC GGATAAAACT TGTGCTTATT TTTCTTTACG GTCTTTAAAA
     3301 AGGCCGTAAT ATCCAGCTGA ACGGTCTGGT TATAGGTACA TTGAGCAACT GACTGAAATG
     3361 CCTCAAAATG TTCTTTACGA TGCCATTGGG ATATATCAAC GGTGGTATAT CCAGTGATTT
     3421 TTTTCTCCAT TTTAGCTTCC TTAGCTCCTG AAAATCTCGA TAACTCAAAA AATACGCCCG
     3481 GTAGTGATCT TATTTCATTA TGGTGAAAGT TGGAACCTCT TACGTGCCCG ATCAACTCGA
     3541 GTGCCACCTG ACGTCTAAGA AACCATTATT ATCATGACAT TAACCTATAA AAATAGGCGT
     3601 ATCACG
//