LOCUS       pEPQD0CM0762_(L0_Cr7DLH 3600 bp ds-DNA     circular     28-SEP-2020
DEFINITION  .
FEATURES             Location/Qualifiers
     primer_bind     28..45
                     /label="M13-fwd"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     CDS             107..1672
                     /label="Translation 107-1672"
     misc_feature    110..1669
                     /label="Cr7DLH 7-deoxyloganic acid hydroxylase CDS, Catharanthus roseus, KF302067  Caros005234 (domesticated, 1 BtgZI and 1 BsmBI site removed)"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    join(1685..3600,1..97)
                     /label="pTwist Chlor High Copy"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     primer_bind     complement(1737..1757)
                     /label="M13-rev"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     rep_origin      complement(1929..2517)
                     /label="pUC origin of replication (derivative of pBR322/pMB1/ColE1)"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     primer_bind     complement(1974..1992)
                     /label="QMD062 primer"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     primer_bind     complement(2009..2028)
                     /label="pBR322ori-F"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     CDS             complement(2765..3424)
                     /label="Chloramphenicol acetyltransferase (CAT) - CamR"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
ORIGIN
        1 GCGCCAGGGT TTTCCCAGTC ACGACGTTGT AAAACGACGG CCAGTCATGC ATAATCCGCA
       61 CGCATCTGGA ATAAGGAAGT GCCATTCCGC CTGACCTAGG TCTCTAATGG AATTGAACTT
      121 CAAATCAATT ATTTTCTTGG TTTTTGTTAG TCTAACCCTT TACTGGGTCT ATAGAATCTT
      181 GGATTGGGTA TGGTTTAAGC CCAAGAAGCT GGAAAAATGC TTAAGAGAAC AAGGGTTTAA
      241 GGGAAATCCT TACAGATTGT TCTTGGGAGA TCAGTATGAT AGTGGAAAAT TGATCAGACA
      301 AGCCTTGACT AAACCTATTG GTGTTGAAGA AGATGTTAAG AAACGAATTG TACCTCATAT
      361 ACTCAAAACT GTTGGGACTC ATGGCAAGAA GTCTTTTATG TGGGTTGGAA GAATACCAAG
      421 GGTGAATATC ACAGATCCAG AGCTGATTAA AGAGGTCTTA ACAAAATACT ATAAGTTCCA
      481 GAAGAATCAT CATGATCTTG ACCCAATTAC CAAATTGCTT TTGACTGGAA TTGGAAGCTT
      541 AGAAGGTGAC CCTTGGGCTA AACGCCGAAA AATCATCAAT GCTGCTTTTC ACTTTGAAAA
      601 GTTAAAGCTT ATGCTTCCTG CATTCTATTT GAGTTGCCGT GATATGGTGA CAAAATGGGA
      661 TAATAAGGTC CCTGAAGGAG GATCAGCAGA GGTGGATGTG TGGCATGATA TTGAAACCTT
      721 AACTGGAGAT GTAATTTCAA GAACATTATT TGGAAGTAAC TTCGAGGAAG GGAGAAGAAT
      781 ATTTGAACTC ATGAAAGAAC TTACTGCTCT TACTATTGAT GTCATTCGCT CGGTTTATAT
      841 CCCTGGACAG AGGTTTCTTC CAACTAAGAG GAACAATAGG ATGAGAGCAA TTGACAAAGA
      901 AGTTAGAGTA AGAATAACAG AAATTATCAA TAAGAAAATG AAGGTAATGA AATCAGGAGA
      961 AGCAGCAAGT GCTGCTGATG ATTTTTTGGG AATATTGTTG GAATGCAATC TGAATGAAAT
     1021 AAAGGAGCAA GGAAATAACA AAAGTGCTGG AATGACTATT GGAGAGATTA TTGGTGAGTG
     1081 CAAACTCTTC TATTTTGCTG GCCAGGACAC TACCTCGACT TTACTCGTGT GGACAATGGT
     1141 CCTCTTGTCT AGATTCCCCG AATGGCAAAC TCGTGCCAGA GAAGAAGTTT TTCAAGTCTT
     1201 TGGTAATAAA ACCCCGGACT ATGATGGTAT CAGCCATCTT AAAGTTATAA CGATGATCTT
     1261 GTACGAGGTT TTGAGGTTAT ATACTCCAGT GGCTGAACTG ACAAAAGTGG CTCATGAAGC
     1321 TACACAACTG GGAAAATACT TCATTCCAGC GGGCGTGCAG CTAATGATGC CACAAATCCT
     1381 ACTTCATCAT GACCCTGAAA TATGGGGTGA AGATGTAATG GAGTTCAAAC CAGAAAGATT
     1441 TGCAGAGGGA GTTCTCAAGG CAACTAAGAG CCAAGGCTCT TTTTTTCCAT TTAGCTTGGG
     1501 ACCAAGAATG TGCATTGGCC AAAACTTTGC TCTATTGGAA GCCAAAATGG CAATGTCTCT
     1561 AATCTTGCGT AGATTTTCTT TCGAGCTTTC TCCCTCCTAT GTTCATGCTC CTTTCACTCT
     1621 CATCACAATG CAACCCCAAT ATGGTGCTCA TTTGATTCTG CACAAACTCT AAGCTTCGAG
     1681 ACCTAGGCTA GGTGGAGGCT CAGTGATGAT AAGTCTGCGA TGGTGGATGC ATGTGTCATG
     1741 GTCATAGCTG TTTCCTGTGT GAAATTGTTA TCCGCTCAGA GGGCACAATC CTATTCCGCG
     1801 CTATCCGACA ATCTCCAAGA CATTAGGTGG AGTTCAGTTC GGCGTATGGC ATATGTCGCT
     1861 GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG
     1921 CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT
     1981 CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC
     2041 CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT
     2101 TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC
     2161 GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA
     2221 TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA
     2281 GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG
     2341 TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG
     2401 CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT
     2461 AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA
     2521 GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCTATTCA ACAAAGCCGC CGTCCCGTCA
     2581 AGTCAGCGTA AATGGGTAGG GGGCTTCAAA TCGTCCCCCC ATACGATATA AGTTGTTACT
     2641 AGTGCTTGGA TTCTCACCAA TAAAAAACGC CCGGCGGCAA CCGAGCGTTC TGAACAAATC
     2701 CAGATGGAGT TCTGAGGTCA TTACTGGATC TATCAACAGG AGTCCAAGCG AGCTCGATAT
     2761 CAAATTACGC CCCGCCCTGC CACTCATCGC AGTACTGTTG TAATTCATTA AGCATTCTGC
     2821 CGACATGGAA GCCATCACAA ACGGCATGAT GAACCTGAAT CGCCAGCGGC ATCAGCACCT
     2881 TGTCGCCTTG CGTATAATAT TTGCCCATGG TGAAAACGGG GGCGAAGAAG TTGTCCATAT
     2941 TGGCCACGTT TAAATCAAAA CTGGTGAAAC TCACCCAGGG ATTGGCTGAG ACGAAAAACA
     3001 TATTCTCAAT AAACCCTTTA GGGAAATAGG CCAGGTTTTC ACCGTAACAC GCCACATCTT
     3061 GCGAATATAT GTGTAGAAAC TGCCGGAAAT CGTCGTGGTA TTCACTCCAG AGCGATGAAA
     3121 ACGTTTCAGT TTGCTCATGG AAAACGGTGT AACAAGGGTG AACACTATCC CATATCACCA
     3181 GCTCACCGTC TTTCATTGCC ATACGAAATT CCGGATGAGC ATTCATCAGG CGGGCAAGAA
     3241 TGTGAATAAA GGCCGGATAA AACTTGTGCT TATTTTTCTT TACGGTCTTT AAAAAGGCCG
     3301 TAATATCCAG CTGAACGGTC TGGTTATAGG TACATTGAGC AACTGACTGA AATGCCTCAA
     3361 AATGTTCTTT ACGATGCCAT TGGGATATAT CAACGGTGGT ATATCCAGTG ATTTTTTTCT
     3421 CCATTTTAGC TTCCTTAGCT CCTGAAAATC TCGATAACTC AAAAAATACG CCCGGTAGTG
     3481 ATCTTATTTC ATTATGGTGA AAGTTGGAAC CTCTTACGTG CCGATCAACG TCTCATTGAT
     3541 ACCTGAAACA AAACCCATCG TACGGCCAAG GAAGTCTCCA ATAACTGTGA TCCACCACAA
//