LOCUS       pEPQD0CM0763_(L0_CrLAMT 3150 bp ds-DNA     circular     28-SEP-2020
DEFINITION  .
FEATURES             Location/Qualifiers
     primer_bind     1..18
                     /label="M13-fwd"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     CDS             80..1195
                     /label="Translation 80-1195"
     misc_feature    83..1192
                     /label="CrLAMT loganic acid O-methyltransferase CDS, Catharanthus roseus, EU057974 (domesticated, 1 BbsI and 1 SapI site removed)"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    join(1208..3150,1..70)
                     /label="pTwist Chlor High Copy"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     primer_bind     complement(1260..1280)
                     /label="M13-rev"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     rep_origin      complement(1452..2040)
                     /label="pUC origin of replication (derivative of pBR322/pMB1/ColE1)"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     primer_bind     complement(1497..1515)
                     /label="QMD062 primer"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     primer_bind     complement(1532..1551)
                     /label="pBR322ori-F"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     CDS             complement(2288..2947)
                     /label="Chloramphenicol acetyltransferase (CAT) - CamR"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
ORIGIN
        1 TGTAAAACGA CGGCCAGTCA TGCATAATCC GCACGCATCT GGAATAAGGA AGTGCCATTC
       61 CGCCTGACCT AGGTCTCTAA TGGTTGCCAC AATTGATTCC ATTGAAATGC CTGCACTTCC
      121 AACAGCTGTG GAAGCCCACC CAATGAAAGG TGGTGATGAC TCCCACAGTT ACTCCCAAAA
      181 TTCTTGCTAC CAGAAAGGAG TAATTGATGC AGCCAAGGCA GTGATTGTTG AAGCAGTGAA
      241 TGAAAAATTG GATCTTGAAA ATAATCCAAT CTTTGATCCA ATCAAACCTT TCCGCATTGC
      301 AGATTTTGGA TGTTCAACAG GACCAAATAC TTTCCACGCT ATGCAAAACA TAGTAGAATC
      361 AGTTGAAACA AAATACAAAT CACTTCAAAA AACCCCTGAA TTCCATGTTT TCTTCAATGA
      421 TCATGTTAAC AATGATTTCA ATGTTCTCTT TAGATCCCTT CCACCAAACA GGGAATTTTT
      481 TGCTGCTGGT GTTCCTGGAT CTTTCTACAC TAGAGTTTTT CCCAAAAATA GCATTCATTT
      541 TGCTCATTGT TCTTATGCAC TTCATTGGTT ATCTAAAGTG CCCAAGGAAA TTCAAGATAA
      601 AAATTCTTTG GCTTATAATA AGGGAAGAAT TCATTACACT GGTACTGAAA AACATGTTGT
      661 TAAGGCTTAT TTTGGTCAAT TCCAAAGAGA TTTTGAAGGG TTTTTAAAAG CAAGAGCTCA
      721 AGAAATTGTT GTTGGAGGGT TGATGGTGAT TCAAATACCT GGGCTTCCTA GTGGTGAAGT
      781 CCTTTTCTCA AGGACTGGTG CTGGTTTGCT TCACTTCCTT TTGGGAACTT CCTTGATGGA
      841 ATTGGTTAAC AAGGGAATCA TCAATGAAGA AAGTGTAGAT TCCTTCAATT TGCCTCAATA
      901 TCATCCCTCA GTTGAAGATT TGGAAATGGT GATAGAAATG AACGATTGTT TCACAATTGA
      961 AAGGGTTGGA ACTTTACCTC ATCCCATGAA GAATTTACCA TTTGATGTTC AAAGGACTTC
     1021 TTTACAAGTA AGAGCAATCA TGGAATGCAT TCTCACTGAA CATTTTGGGG AAAATATTTT
     1081 GGATCCATTA TTTGAAATCT ACACCAAAAA TTTGCAAGAG AATTTCCATG TTTTTGATAA
     1141 GGAAATTAGA AAGGATGCAG ATTTGTACCT TGTCTTGAAA CGCAAGGGAA ATTAAGCTTC
     1201 GAGACCTAGG CTAGGTGGAG GCTCAGTGAT GATAAGTCTG CGATGGTGGA TGCATGTGTC
     1261 ATGGTCATAG CTGTTTCCTG TGTGAAATTG TTATCCGCTC AGAGGGCACA ATCCTATTCC
     1321 GCGCTATCCG ACAATCTCCA AGACATTAGG TGGAGTTCAG TTCGGCGTAT GGCATATGTC
     1381 GCTGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG
     1441 TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA
     1501 AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC
     1561 TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC
     1621 CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG
     1681 GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC
     1741 TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA
     1801 GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG
     1861 AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG
     1921 AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT
     1981 GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA
     2041 GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCTAT TCAACAAAGC CGCCGTCCCG
     2101 TCAAGTCAGC GTAAATGGGT AGGGGGCTTC AAATCGTCCC CCCATACGAT ATAAGTTGTT
     2161 ACTAGTGCTT GGATTCTCAC CAATAAAAAA CGCCCGGCGG CAACCGAGCG TTCTGAACAA
     2221 ATCCAGATGG AGTTCTGAGG TCATTACTGG ATCTATCAAC AGGAGTCCAA GCGAGCTCGA
     2281 TATCAAATTA CGCCCCGCCC TGCCACTCAT CGCAGTACTG TTGTAATTCA TTAAGCATTC
     2341 TGCCGACATG GAAGCCATCA CAAACGGCAT GATGAACCTG AATCGCCAGC GGCATCAGCA
     2401 CCTTGTCGCC TTGCGTATAA TATTTGCCCA TGGTGAAAAC GGGGGCGAAG AAGTTGTCCA
     2461 TATTGGCCAC GTTTAAATCA AAACTGGTGA AACTCACCCA GGGATTGGCT GAGACGAAAA
     2521 ACATATTCTC AATAAACCCT TTAGGGAAAT AGGCCAGGTT TTCACCGTAA CACGCCACAT
     2581 CTTGCGAATA TATGTGTAGA AACTGCCGGA AATCGTCGTG GTATTCACTC CAGAGCGATG
     2641 AAAACGTTTC AGTTTGCTCA TGGAAAACGG TGTAACAAGG GTGAACACTA TCCCATATCA
     2701 CCAGCTCACC GTCTTTCATT GCCATACGAA ATTCCGGATG AGCATTCATC AGGCGGGCAA
     2761 GAATGTGAAT AAAGGCCGGA TAAAACTTGT GCTTATTTTT CTTTACGGTC TTTAAAAAGG
     2821 CCGTAATATC CAGCTGAACG GTCTGGTTAT AGGTACATTG AGCAACTGAC TGAAATGCCT
     2881 CAAAATGTTC TTTACGATGC CATTGGGATA TATCAACGGT GGTATATCCA GTGATTTTTT
     2941 TCTCCATTTT AGCTTCCTTA GCTCCTGAAA ATCTCGATAA CTCAAAAAAT ACGCCCGGTA
     3001 GTGATCTTAT TTCATTATGG TGAAAGTTGG AACCTCTTAC GTGCCGATCA ACGTCTCATT
     3061 GATACCTGAA ACAAAACCCA TCGTACGGCC AAGGAAGTCT CCAATAACTG TGATCCACCA
     3121 CAAGCGCCAG GGTTTTCCCA GTCACGACGT
//