LOCUS       pEPQD0CM0764_(L0_CrSLS_ 3618 bp ds-DNA     circular     28-SEP-2020
DEFINITION  .
FEATURES             Location/Qualifiers
     primer_bind     1..18
                     /label="M13-fwd"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     CDS             80..1663
                     /label="Translation 80-1663"
     misc_feature    83..1660
                     /label="name depends on ref: SLS1 Brown et al. PNAS 112(11) 3205-3210 (2015), SLS2 de Bernonville et al. BMC Genomics 16(1) 619 (2015)"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_feature    83..1660
                     /label="CrSLS secologanin synthase, Catharanthus roseus KF309242.1 (domesticated, 1 SapI site removed))"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    join(1676..3618,1..70)
                     /label="pTwist Chlor High Copy"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     primer_bind     complement(1728..1748)
                     /label="M13-rev"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     rep_origin      complement(1920..2508)
                     /label="pUC origin of replication (derivative of pBR322/pMB1/ColE1)"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     primer_bind     complement(1965..1983)
                     /label="QMD062 primer"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     primer_bind     complement(2000..2019)
                     /label="pBR322ori-F"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     CDS             complement(2756..3415)
                     /label="Chloramphenicol acetyltransferase (CAT) - CamR"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
ORIGIN
        1 TGTAAAACGA CGGCCAGTCA TGCATAATCC GCACGCATCT GGAATAAGGA AGTGCCATTC
       61 CGCCTGACCT AGGTCTCTAA TGGAGATGGA TATGGATATC ATTAGAAAGG CAATTGCTGC
      121 CACTATCTTT GCATTGGTTA TGGCTTGGGC GTGGAGAGTG TTGGATTGGG CATGGTTTAC
      181 TCCTAAGAGG ATCGAGAAAC GTCTAAGGCA GCAAGGTTTT AGAGGAAATC CTTATAGATT
      241 CTTGGTTGGA GATGTTAAGG AGAGTGGAAA AATGCATCAA GAAGCCTTGT CTAACCCCAT
      301 GGAGTTCGAC AACGATATTG TTCCTCGCCT CATGCCACAT ATTAACCACA CCATAAAAAC
      361 TTACGGTAGG AATTCATTTA CATGGATGGG AAGGATTCCA AGGATTCATG TTATGGAACC
      421 TGAACTTATT AAGGAAGTAT TGACCCACTC CAGCAAATAC CAAAAGAACT TTGATGTTCA
      481 CAATCCCCTT GTTAAGTTCC TTCTCACTGG AGTTGGAAGC TTTGAAGGGG CAAAATGGTC
      541 CAAACACAGA AGAATCATTT CCCCTGCCTT CACTCTTGAG AAACTAAAGT CAATGTTGCC
      601 GGCTTTTGCC ATATGCTATC ATGACATGTT GACGAAATGG GAGAAATTAG CAGAAAAAGA
      661 AGGATCCCAT GAAGTTGATA TCTTCCCCAC ATTTGATGTT TTGACAAGTG ATGTAATTTC
      721 AAAGGTTGCA TTTGGTAGCA CATACGATGA AGGAGGCAAA ATCTTCAGAC TATTGAAAGA
      781 ACTCATGGAT CTCACAATTG ATTGCATGAG AGATGTCTAC ATCCCAGGGT GGAGCTACTT
      841 GCCAACCAAG AGGAACAAGA GGATGAAAGA AATTAACAAA GAAATCACAG ATATGCTAAG
      901 GTTCATCATC AACAAGAGAA TGAAGGCTTT GAAGGCTGGA GAACCAGGTG AGGATGACTT
      961 GTTGGGAGTC CTGTTGGAAT CAAACATTCA AGAAATTCAG AAACAAGGAA ACAGGAAGGA
     1021 TGGTGGAATG ACAATCAACG ATGTGATTGA GGAATGCAAA TTGTTCTACT TTGCTGGTCA
     1081 AGAAACCACC GGAGTTTTGC TCACATGGAC CACCATTTTA TTGAGCAAGC ATCCCGAGTG
     1141 GCAAGAGCGA GCTAGGGAAG AAGTTCTCCA AGCCTTTGGC AAGAACAAGC CTGAATTTGA
     1201 ACGCTTGAAT CATCTTAAAT ATGTTTCTAT GATCTTATAC GAGGTTCTAA GGTTGTACCC
     1261 GCCAGTTATT GATCTGACCA AGATTATTCA CGAGGACACA AAGTTAGGGC CGTACACAAT
     1321 CCCTGCAGGA ACACAAGTAA TGTTGCCGAC AGTAATGCTT CATAGAGAGA AGTCCATTTG
     1381 GGGAGAAGAT GCAATGGAAT TCAACCCAAT GAGATTTGCT GATGGAGTTG CCAATGCCAC
     1441 CAAGAACAAT GTCACATATT TGCCATTCAG TTGGGGACCT AGAGTTTGCC TCGGCCAAAA
     1501 CTTTGCACTT CTTCAAGCAA AATTAGGGTT GGCAATGATT TTACAACGCT TCAAATTTGA
     1561 TGTAGCTCCA TCCTATGTTC ATGCTCCCTT TACCATTCTT ACAGTTCAAC CTCAGTTTGG
     1621 TTCTCATGTT ATCTACAAGA AGCTTGAGAG ACAGAATTTT TAAGCTTCGA GACCTAGGCT
     1681 AGGTGGAGGC TCAGTGATGA TAAGTCTGCG ATGGTGGATG CATGTGTCAT GGTCATAGCT
     1741 GTTTCCTGTG TGAAATTGTT ATCCGCTCAG AGGGCACAAT CCTATTCCGC GCTATCCGAC
     1801 AATCTCCAAG ACATTAGGTG GAGTTCAGTT CGGCGTATGG CATATGTCGC TGGAAAGAAC
     1861 ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT
     1921 TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG
     1981 CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC
     2041 TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC
     2101 GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC
     2161 AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC
     2221 TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT
     2281 AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT
     2341 AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC
     2401 TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT
     2461 TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG
     2521 ATCTTTTCTA CGGGGTCTGA CGCTCTATTC AACAAAGCCG CCGTCCCGTC AAGTCAGCGT
     2581 AAATGGGTAG GGGGCTTCAA ATCGTCCCCC CATACGATAT AAGTTGTTAC TAGTGCTTGG
     2641 ATTCTCACCA ATAAAAAACG CCCGGCGGCA ACCGAGCGTT CTGAACAAAT CCAGATGGAG
     2701 TTCTGAGGTC ATTACTGGAT CTATCAACAG GAGTCCAAGC GAGCTCGATA TCAAATTACG
     2761 CCCCGCCCTG CCACTCATCG CAGTACTGTT GTAATTCATT AAGCATTCTG CCGACATGGA
     2821 AGCCATCACA AACGGCATGA TGAACCTGAA TCGCCAGCGG CATCAGCACC TTGTCGCCTT
     2881 GCGTATAATA TTTGCCCATG GTGAAAACGG GGGCGAAGAA GTTGTCCATA TTGGCCACGT
     2941 TTAAATCAAA ACTGGTGAAA CTCACCCAGG GATTGGCTGA GACGAAAAAC ATATTCTCAA
     3001 TAAACCCTTT AGGGAAATAG GCCAGGTTTT CACCGTAACA CGCCACATCT TGCGAATATA
     3061 TGTGTAGAAA CTGCCGGAAA TCGTCGTGGT ATTCACTCCA GAGCGATGAA AACGTTTCAG
     3121 TTTGCTCATG GAAAACGGTG TAACAAGGGT GAACACTATC CCATATCACC AGCTCACCGT
     3181 CTTTCATTGC CATACGAAAT TCCGGATGAG CATTCATCAG GCGGGCAAGA ATGTGAATAA
     3241 AGGCCGGATA AAACTTGTGC TTATTTTTCT TTACGGTCTT TAAAAAGGCC GTAATATCCA
     3301 GCTGAACGGT CTGGTTATAG GTACATTGAG CAACTGACTG AAATGCCTCA AAATGTTCTT
     3361 TACGATGCCA TTGGGATATA TCAACGGTGG TATATCCAGT GATTTTTTTC TCCATTTTAG
     3421 CTTCCTTAGC TCCTGAAAAT CTCGATAACT CAAAAAATAC GCCCGGTAGT GATCTTATTT
     3481 CATTATGGTG AAAGTTGGAA CCTCTTACGT GCCGATCAAC GTCTCATTGA TACCTGAAAC
     3541 AAAACCCATC GTACGGCCAA GGAAGTCTCC AATAACTGTG ATCCACCACA AGCGCCAGGG
     3601 TTTTCCCAGT CACGACGT
//