LOCUS       pEPQD0CM0765_(L0_CrTDC_ 3537 bp ds-DNA     circular     28-SEP-2020
DEFINITION  .
FEATURES             Location/Qualifiers
     primer_bind     1..18
                     /label="M13-fwd"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     CDS             80..1582
                     /label="Translation 80-1582"
     None            83..1579
                     /label="CrTDC tryptophan decarboxylase CDS, Catharanthus roseus, M25151 (domesticated, 1 BtgZI site removed)"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    join(1595..3537,1..70)
                     /label="pTwist Chlor High Copy"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     primer_bind     complement(1647..1667)
                     /label="M13-rev"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     rep_origin      complement(1839..2427)
                     /label="pUC origin of replication (derivative of pBR322/pMB1/ColE1)"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     primer_bind     complement(1884..1902)
                     /label="QMD062 primer"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     primer_bind     complement(1919..1938)
                     /label="pBR322ori-F"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     CDS             complement(2675..3334)
                     /label="Chloramphenicol acetyltransferase (CAT) - CamR"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
ORIGIN
        1 TGTAAAACGA CGGCCAGTCA TGCATAATCC GCACGCATCT GGAATAAGGA AGTGCCATTC
       61 CGCCTGACCT AGGTCTCTAA TGGGCAGCAT TGATTCAACA AATGTAGCCA TGTCCAATTC
      121 TCCAGTTGGA GAATTTAAGC CACTTGAAGC TGAGGAATTC CGAAAACAAG CCCATCGTAT
      181 GGTAGATTTC ATAGCCGATT ATTACAAAAA TGTGGAAACA TATCCGGTCC TTAGCGAAGT
      241 CGAACCTGGA TATCTCCGAA AACGTATCCC CGAAACCGCT CCTTACCTCC CCGAACCACT
      301 TGACGACATC ATGAAAGATA TTCAGAAGGA TATTATCCCA GGAATGACAA ATTGGATGAG
      361 CCCTAATTTT TATGCATTTT TTCCTGCCAC TGTTAGTTCA GCTGCCTTTT TAGGAGAAAT
      421 GTTGTCTACT GCCCTAAATT CAGTAGGCTT TACTTGGGTT TCTTCACCAG CCGCCACCGA
      481 ATTAGAAATG ATTGTTATGG ATTGGTTGGC TCAGATCCTT AAACTCCCCA AATCTTTCAT
      541 GTTTTCAGGT ACCGGTGGCG GCGTCATCCA AAACACCACT AGCGAGTCCA TTCTTTGTAC
      601 AATCATTGCC GCCCGGGAAA GGGCCCTGGA GAAGCTCGGT CCCGATAGTA TTGGAAAACT
      661 TGTCTGTTAC GGATCCGATC AAACCCATAC CATGTTCCCC AAAACTTGCA AATTGGCGGG
      721 AATTTATCCG AATAATATTA GGTTAATACC TACGACCGTC GAAACGGATT TCGGCATCTC
      781 ACCTCAAGTT CTACGAAAAA TGGTCGAGGA TGACGTGGCG GCCGGATATG TACCGCTGTT
      841 CTTATGCGCT ACCCTGGGTA CCACCTCGAC CACGGCTACC GATCCTGTGG ACTCACTTTC
      901 TGAAATCGCT AACGAGTTTG GTATTTGGAT CCACGTGGAT GCTGCTTATG CGGGAAGCGC
      961 CTGTATATGT CCCGAGTTTA GACATTACTT GGATGGAATC GAACGAGTTG ACTCACTGAG
     1021 TCTGAGTCCA CACAAATGGC TACTCGCTTA CTTAGATTGC ACTTGCTTGT GGGTCAAGCA
     1081 ACCACATTTG TTACTAAGGG CACTCACTAC GAATCCTGAG TATTTAAAAA ATAAACAGAG
     1141 TGATTTAGAC AAAGTTGTGG ACTTCAAAAA TTGGCAAATC GCAACGGGAC GAAAATTTCG
     1201 GTCGCTGAAA CTTTGGCTCA TTTTACGTAG CTATGGAGTT GTTAATTTAC AGAGTCATAT
     1261 TCGTTCTGAC GTCGCAATGG GCAAAATGTT CGAAGAATGG GTTAGATCAG ACTCCAGATT
     1321 CGAAATTGTG GTACCGAGAA ACTTTTCTCT TGTTTGTTTT AGATTAAAAC CTGACGTTTC
     1381 GAGTTTACAT GTAGAAGAAG TGAATAAGAA ACTTTTGGAC ATGCTTAACT CGACGGGACG
     1441 AGTTTATATG ACTCATACTA TTGTGGGAGG CATATACATG CTAAGACTGG CTGTTGGCTC
     1501 GTCACTAACT GAAGAACATC ATGTACGCCG TGTTTGGGAT TTGATTCAAA AATTAACCGA
     1561 TGATTTGCTC AAAGAAGCTT GAGCTTCGAG ACCTAGGCTA GGTGGAGGCT CAGTGATGAT
     1621 AAGTCTGCGA TGGTGGATGC ATGTGTCATG GTCATAGCTG TTTCCTGTGT GAAATTGTTA
     1681 TCCGCTCAGA GGGCACAATC CTATTCCGCG CTATCCGACA ATCTCCAAGA CATTAGGTGG
     1741 AGTTCAGTTC GGCGTATGGC ATATGTCGCT GGAAAGAACA TGTGAGCAAA AGGCCAGCAA
     1801 AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT
     1861 GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA
     1921 AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG
     1981 CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA
     2041 CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA
     2101 CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG
     2161 GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG
     2221 TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA
     2281 ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC
     2341 TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG
     2401 ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC
     2461 GCTCTATTCA ACAAAGCCGC CGTCCCGTCA AGTCAGCGTA AATGGGTAGG GGGCTTCAAA
     2521 TCGTCCCCCC ATACGATATA AGTTGTTACT AGTGCTTGGA TTCTCACCAA TAAAAAACGC
     2581 CCGGCGGCAA CCGAGCGTTC TGAACAAATC CAGATGGAGT TCTGAGGTCA TTACTGGATC
     2641 TATCAACAGG AGTCCAAGCG AGCTCGATAT CAAATTACGC CCCGCCCTGC CACTCATCGC
     2701 AGTACTGTTG TAATTCATTA AGCATTCTGC CGACATGGAA GCCATCACAA ACGGCATGAT
     2761 GAACCTGAAT CGCCAGCGGC ATCAGCACCT TGTCGCCTTG CGTATAATAT TTGCCCATGG
     2821 TGAAAACGGG GGCGAAGAAG TTGTCCATAT TGGCCACGTT TAAATCAAAA CTGGTGAAAC
     2881 TCACCCAGGG ATTGGCTGAG ACGAAAAACA TATTCTCAAT AAACCCTTTA GGGAAATAGG
     2941 CCAGGTTTTC ACCGTAACAC GCCACATCTT GCGAATATAT GTGTAGAAAC TGCCGGAAAT
     3001 CGTCGTGGTA TTCACTCCAG AGCGATGAAA ACGTTTCAGT TTGCTCATGG AAAACGGTGT
     3061 AACAAGGGTG AACACTATCC CATATCACCA GCTCACCGTC TTTCATTGCC ATACGAAATT
     3121 CCGGATGAGC ATTCATCAGG CGGGCAAGAA TGTGAATAAA GGCCGGATAA AACTTGTGCT
     3181 TATTTTTCTT TACGGTCTTT AAAAAGGCCG TAATATCCAG CTGAACGGTC TGGTTATAGG
     3241 TACATTGAGC AACTGACTGA AATGCCTCAA AATGTTCTTT ACGATGCCAT TGGGATATAT
     3301 CAACGGTGGT ATATCCAGTG ATTTTTTTCT CCATTTTAGC TTCCTTAGCT CCTGAAAATC
     3361 TCGATAACTC AAAAAATACG CCCGGTAGTG ATCTTATTTC ATTATGGTGA AAGTTGGAAC
     3421 CTCTTACGTG CCGATCAACG TCTCATTGAT ACCTGAAACA AAACCCATCG TACGGCCAAG
     3481 GAAGTCTCCA ATAACTGTGA TCCACCACAA GCGCCAGGGT TTTCCCAGTC ACGACGT
//