LOCUS       pEPQD0CM0766_(L0_CrSTR_ 3093 bp ds-DNA     circular     28-SEP-2020
DEFINITION  .
FEATURES             Location/Qualifiers
     primer_bind     2..19
                     /label="M13-fwd"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     CDS             81..1139
                     /label="Translation 81-1139"
     misc_feature    84..1136
                     /label="CrSTR2 strictosidine synthase, Catharanthus roseus, X61932 (domesticated, 1 BbsI site removed, L19-S32 recoded to avoid repeats)"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    join(1152..3093,1..71)
                     /label="pTwist Chlor High Copy"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     primer_bind     complement(1204..1224)
                     /label="M13-rev"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     rep_origin      complement(1396..1984)
                     /label="pUC origin of replication (derivative of pBR322/pMB1/ColE1)"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     primer_bind     complement(1441..1459)
                     /label="QMD062 primer"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     primer_bind     complement(1476..1495)
                     /label="pBR322ori-F"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     CDS             complement(2232..2891)
                     /label="Chloramphenicol acetyltransferase (CAT) - CamR"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
ORIGIN
        1 TTGTAAAACG ACGGCCAGTC ATGCATAATC CGCACGCATC TGGAATAAGG AAGTGCCATT
       61 CCGCCTGACC TAGGTCTCTA ATGGCAAACT TTTCTGAATC TAAATCCATG ATGGCAGTTT
      121 TCTTCATGTT TTTCCTGCTT CTTTTGTCAT CTTCTAGTTC TTCATCTTCA AGTTCACCAA
      181 TTTTGAAAAA GATTTTTATT GAAAGCCCTT CCTATGCTCC GAATGCCTTC ACCTTCGATT
      241 CAACTGATAA AGGGTTCTAC ACTTCCGTCC AAGATGGCCG AGTTATCAAA TATGAAGGGC
      301 CAAATTCAGG CTTCACTGAC TTCGCCTACG CATCTCCCTT CTGGAACAAA GCTTTTTGTG
      361 AGAACAGCAC CGATCCAGAG AAAAGACCAT TGTGTGGGAG GACATATGAT ATTTCCTATG
      421 ACTATAAGAA CAGCCAAATG TACATTGTTG ATGGCCATTA CCATCTTTGT GTGGTTGGAA
      481 AAGAAGGTGG GTATGCCACA CAACTAGCCA CAAGTGTGCA AGGAGTGCCA TTCAAATGGC
      541 TCTATGCAGT AACTGTTGAT CAGAGAACAG GGATTGTTTA TTTCACTGAT GTTAGCTCCA
      601 TACATGATGA CAGTCCCGAA GGTGTGGAAG AAATCATGAA TACAAGTGAT AGAACAGGGA
      661 GATTAATGAA GTATGATCCT TCAACAAAAG AAACCACCTT ATTATTGAAA GAGCTACATG
      721 TTCCCGGCGG TGCAGAAATC AGCGCAGATG GTTCCTTTGT TGTAGTAGCA GAATTTTTAA
      781 GCAATCGGAT AGTGAAGTAT TGGCTAGAAG GGCCAAAGAA AGGCAGTGCA GAGTTCTTAG
      841 TTACAATCCC AAATCCAGGA AATATAAAGA GGAATTCTGA TGGCCATTTT TGGGTGTCAT
      901 CTAGTGAAGA ATTAGATGGA GGTCAACATG GAAGAGTTGT TTCAAGAGGA ATTAAGTTTG
      961 ATGGATTTGG GAATATTCTT CAAGTTATAC CACTTCCACC ACCATATGAA GGTGAACATT
     1021 TTGAACAGAT TCAAGAGCAC GATGGTTTGT TATACATTGG AAGTCTCTTC CATAGCTCTG
     1081 TGGGTATATT AGTGTATGAT GATCATGATA ACAAGGGAAA TTCTTATGTT TCTAGCTGAG
     1141 CTTCGAGACC TAGGCTAGGT GGAGGCTCAG TGATGATAAG TCTGCGATGG TGGATGCATG
     1201 TGTCATGGTC ATAGCTGTTT CCTGTGTGAA ATTGTTATCC GCTCAGAGGG CACAATCCTA
     1261 TTCCGCGCTA TCCGACAATC TCCAAGACAT TAGGTGGAGT TCAGTTCGGC GTATGGCATA
     1321 TGTCGCTGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC
     1381 CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG
     1441 CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG
     1501 AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT
     1561 TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT
     1621 GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG
     1681 CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT
     1741 GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT
     1801 CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT
     1861 GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC
     1921 CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC
     1981 TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CTATTCAACA AAGCCGCCGT
     2041 CCCGTCAAGT CAGCGTAAAT GGGTAGGGGG CTTCAAATCG TCCCCCCATA CGATATAAGT
     2101 TGTTACTAGT GCTTGGATTC TCACCAATAA AAAACGCCCG GCGGCAACCG AGCGTTCTGA
     2161 ACAAATCCAG ATGGAGTTCT GAGGTCATTA CTGGATCTAT CAACAGGAGT CCAAGCGAGC
     2221 TCGATATCAA ATTACGCCCC GCCCTGCCAC TCATCGCAGT ACTGTTGTAA TTCATTAAGC
     2281 ATTCTGCCGA CATGGAAGCC ATCACAAACG GCATGATGAA CCTGAATCGC CAGCGGCATC
     2341 AGCACCTTGT CGCCTTGCGT ATAATATTTG CCCATGGTGA AAACGGGGGC GAAGAAGTTG
     2401 TCCATATTGG CCACGTTTAA ATCAAAACTG GTGAAACTCA CCCAGGGATT GGCTGAGACG
     2461 AAAAACATAT TCTCAATAAA CCCTTTAGGG AAATAGGCCA GGTTTTCACC GTAACACGCC
     2521 ACATCTTGCG AATATATGTG TAGAAACTGC CGGAAATCGT CGTGGTATTC ACTCCAGAGC
     2581 GATGAAAACG TTTCAGTTTG CTCATGGAAA ACGGTGTAAC AAGGGTGAAC ACTATCCCAT
     2641 ATCACCAGCT CACCGTCTTT CATTGCCATA CGAAATTCCG GATGAGCATT CATCAGGCGG
     2701 GCAAGAATGT GAATAAAGGC CGGATAAAAC TTGTGCTTAT TTTTCTTTAC GGTCTTTAAA
     2761 AAGGCCGTAA TATCCAGCTG AACGGTCTGG TTATAGGTAC ATTGAGCAAC TGACTGAAAT
     2821 GCCTCAAAAT GTTCTTTACG ATGCCATTGG GATATATCAA CGGTGGTATA TCCAGTGATT
     2881 TTTTTCTCCA TTTTAGCTTC CTTAGCTCCT GAAAATCTCG ATAACTCAAA AAATACGCCC
     2941 GGTAGTGATC TTATTTCATT ATGGTGAAAG TTGGAACCTC TTACGTGCCG ATCAACGTCT
     3001 CATTGATACC TGAAACAAAA CCCATCGTAC GGCCAAGGAA GTCTCCAATA ACTGTGATCC
     3061 ACCACAAGCG CCAGGGTTTT CCCAGTCACG ACG
//