LOCUS       pEPQD0CM0910_(L0_MsLAMT 3183 bp ds-DNA     circular     28-MAY-2021
DEFINITION  .
FEATURES             Location/Qualifiers
     Terminator      22..116
                     /label="lambda t0 terminator"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     CDS             complement(137..796)
                     /label="Chloramphenicol acetyltransferase (CAT) - CamR"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     primer          711..730
                     /label="QMD463_CATseq_R"
                     /note="sequence: gcaactgactgaaatgcctc"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_feature    962..984
                     /label="pUAP/DP2-F"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     primer          962..984
                     /label="pUAP/DP2-F"
                     /note="sequence: ggcgtatcacgaggcagaatttc"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     CDS             1040..2164
                     /label="Translation 1040-2164"
     misc_feature    1043..2161
                     /label="MsLAMT loganic acid O-methyltransferase CDS, from Mitragyna speciosa (kratom) - domesticated (1 BbsI site removed), note Q262 removed compared to reference sequence from O'Connor lab (Yindi)"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    complement(1731..1736)
                     /label="domesticated BbsI"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    complement(2191..2211)
                     /label="pUAP/DP2-R"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     primer          complement(2191..2211)
                     /label="pUAP/DP2-R"
                     /note="sequence: ggtggtgacaccttgcccttt"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     primer          complement(2238..2258)
                     /label="QMD499_pUAP/DP2-R2"
                     /note="sequence: CCTGCATAACGCGAAGTAATC"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     primer          complement(2338..2363)
                     /label="211or (L0 FWD)"
                     /note="sequence: CGTTATCCCCTGATTCTGTGGATAAC"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer          2340..2362
                     /label="QMD542_211or_binding_seq_R"
                     /note="sequence: TATCCACAGAATCAGGGGATAAC"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     Replication_ori complement(2434..3022)
                     /label="origin of replication (derivative of pUC/pBR322/pMB1/colE1), T-->C at 3' end, pUAP vectors"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    2441..3022
                     /label="matches pUC origin of replication"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     primer          complement(2479..2497)
                     /label="QMD062_pJL1_outside(BR322)_F"
                     /note="sequence: tcgccacctctgacttgag"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer          complement(2481..2500)
                     /label="QMD132_LETprom2_F, 61.4"
                     /note="sequence: gtttcgccacctctgacttg"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer          complement(2510..2533)
                     /label="QMD131_LETprom1_F, 61.7, -3.89 self"
                     /note="sequence: gggaaacgcctggtatctttatag"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     primer          2979..3000
                     /label="QMD540_pBR322_seq_R"
                     /note="sequence: ttgtttgcaagcagcagattac"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
ORIGIN
        1 GTCTGACAGC TCGAGGCTTG GATTCTCACC AATAAAAAAC GCCCGGCGGC AACCGAGCGT
       61 TCTGAACAAA TCCAGATGGA GTTCTGAGGT CATTACTGGA TCTATCAACA GGAGTCCAAG
      121 CGAGCTCGAT ATCAAATTAC GCCCCGCCCT GCCACTCATC GCAGTACTGT TGTAATTCAT
      181 TAAGCATTCT GCCGACATGG AAGCCATCAC AAACGGCATG ATGAACCTGA ATCGCCAGCG
      241 GCATCAGCAC CTTGTCGCCT TGCGTATAAT ATTTGCCCAT GGTGAAAACG GGGGCGAAGA
      301 AGTTGTCCAT ATTGGCCACG TTTAAATCAA AACTGGTGAA ACTCACCCAG GGATTGGCTG
      361 AGACGAAAAA CATATTCTCA ATAAACCCTT TAGGGAAATA GGCCAGGTTT TCACCGTAAC
      421 ACGCCACATC TTGCGAATAT ATGTGTAGAA ACTGCCGGAA ATCGTCGTGG TATTCACTCC
      481 AGAGCGATGA AAACGTTTCA GTTTGCTCAT GGAAAACGGT GTAACAAGGG TGAACACTAT
      541 CCCATATCAC CAGCTCACCG TCTTTCATTG CCATACGAAA TTCCGGATGA GCATTCATCA
      601 GGCGGGCAAG AATGTGAATA AAGGCCGGAT AAAACTTGTG CTTATTTTTC TTTACGGTCT
      661 TTAAAAAGGC CGTAATATCC AGCTGAACGG TCTGGTTATA GGTACATTGA GCAACTGACT
      721 GAAATGCCTC AAAATGTTCT TTACGATGCC ATTGGGATAT ATCAACGGTG GTATATCCAG
      781 TGATTTTTTT CTCCATTTTA GCTTCCTTAG CTCCTGAAAA TCTCGATAAC TCAAAAAATA
      841 CGCCCGGTAG TGATCTTATT TCATTATGGT GAAAGTTGGA ACCTCTTACG TGCCCGATCA
      901 ACTCGAGTGC CACCTGACGT CTAAGAAACC ATTATTATCA TGACATTAAC CTATAAAAAT
      961 AGGCGTATCA CGAGGCAGAA TTTCAGATAA AAAAAATCCT TAGCTTTCGC TAAGGATGAT
     1021 TTCTGGAATT CGGTCTCAAa tgGCCCCAAC ACTGGACACC ATCCCAACTG TAGATGTCAA
     1081 GGAAATGCCT GAAGAAGCGC ATCCTATGAA AGGCGGTGAT GACTTCAATA GCTATTCTCA
     1141 GAATTCTTGC TATCAGAGGG GAGTTATAGA TGCTGCAAAA GTAGTTATCG TTGAAGCAGT
     1201 AAATGAAAAG CTGGATCTAA AGAATAATGC TAATTTTGAT CCATCTAAGC CTTTCCACAT
     1261 TGCTGATTTT GGGTGCTCAA CCGGCCCTAA CACATATTTT GCCGTGCAAA ATATTGTAGA
     1321 AGCCGTGGAC CAAAAACACA AATCCCTTCA CGAAAAAATC CAGCCAGAAT TCCATGTTTT
     1381 TTTCAATGAT CATGTCAACA ATGATTTCAA CATTCTCTTC AGATCTCTCC CACAGGACAG
     1441 GGATTATTTT GCTGCTGGGG TCCCTGGATC ATTTTACACA AGGGTTTTTC CCAAGGCCAG
     1501 TGTACATTTT GCTCATTGCT CGTATGCACT TCACTGGCTG TCCAAGGTTC CTAAGGAAAT
     1561 TCAGGACAAG AACTCTCCTG CCTTTAACAG AGGGAGAATT CATTACACTG GGACTGAAAA
     1621 ACATGTCGTG AAAGCTTACT TTTCTCAATT CCAAAAAGAC ATGGGCTCAT TTCTGAAAGC
     1681 TAGAGCTCAA GAAATTGTGG GAGGAGGGTT GGTGGTGATT CAAATTCCCG GACTTCCAAG
     1741 TGGTGAGGTC CTCTTCTCTA GGACCGGTGC TGGCATGCTT CATGCTCTTT TGGGATCTTC
     1801 CTTGATGGAA CTCGTAAATT TGGGAATAAT AACCGAAGAA AGTGTGGACT CCTTCAACTT
     1861 GCCTCAATAC CATCCATCAA TTGAGGAATT GGAAATGGTG ATAGAGATGA ACAATAGTTT
     1921 TACCATTGAA AAAGTTGGAG CATTGAATCA CCCCATGAAG AACTTACCAT TTGACGTTCA
     1981 AATGACTTCT CTGCAAATAA GAGCTGTCAT GGAGGGGATT CTGTCTGATC ATTTTGGAGG
     2041 AAAAATCTTG GATCAATTGT TCGAAATTTA CACCACGAAA CTGCAAGAAA ATTACAACGT
     2101 TTTCGACAAG GAAATCCGAA GGGATGCTGA TTTGTTCCTT GTTCTAAAAC GTAAGAGCAA
     2161 TtgaGCTTCG AGACCCTGCA GTCCGGCAAA AAAGGGCAAG GTGTCACCAC CCTGCCCTTT
     2221 TTCTTTAAAA CCGAAAAGAT TACTTCGCGT TATGCAGGCT TCCTCGCTCA CTGACTCGCT
     2281 GCGCTCGGTC GTTCGGCTGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT
     2341 ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC
     2401 CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAC AGGCTCCGCC CCCCTGACGA
     2461 GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA
     2521 CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC
     2581 CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG
     2641 TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC
     2701 CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG
     2761 ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT
     2821 AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT
     2881 ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG
     2941 ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC
     3001 GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA
     3061 GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC
     3121 CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAAAC
     3181 TTG
//