LOCUS       pEPQD0CM0812_(pUAP-pGal 2340 bp ds-DNA     circular     27-OCT-2020
DEFINITION  .
FEATURES             Location/Qualifiers
     Terminator      111..205
                     /label="lambda t0 terminator"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     CDS             complement(226..885)
                     /label="CmR"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     primer          800..819
                     /label="QMD463_CATseq_R"
                     /note="sequence: gcaactgactgaaatgcctc"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     primer          1051..1073
                     /label="pUAP/DP2-F"
                     /note="sequence: ggcgtatcacgaggcagaatttc"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     CDS             1130..1414
                     /label="Translation 1130-1414"
     CDS             1133..1381
                     /label="Gal4AD"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     CDS             1382..1410
                     /label="Linker"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     primer          complement(1437..1457)
                     /label="pUAP/DP2-R"
                     /note="sequence: ggtggtgacaccttgcccttt"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     primer          complement(1484..1504)
                     /label="QMD499_pUAP/DP2-R2"
                     /note="sequence: CCTGCATAACGCGAAGTAATC"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     primer          complement(1584..1609)
                     /label="211or (L0 FWD)"
                     /note="sequence: CGTTATCCCCTGATTCTGTGGATAAC"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer          1586..1608
                     /label="QMD542_211or_binding_seq_R"
                     /note="sequence: TATCCACAGAATCAGGGGATAAC"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     Replication_ori complement(1680..2268)
                     /label="ori"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     primer          complement(1725..1743)
                     /label="QMD062_pJL1_outside(BR322)_F"
                     /note="sequence: tcgccacctctgacttgag"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer          complement(1727..1746)
                     /label="QMD132_LETprom2_F, 61.4"
                     /note="sequence: gtttcgccacctctgacttg"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer          complement(1756..1779)
                     /label="QMD131_LETprom1_F, 61.7, -3.89 self"
                     /note="sequence: gggaaacgcctggtatctttatag"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     primer          2225..2246
                     /label="QMD540_pBR322_seq_R"
                     /note="sequence: ttgtttgcaagcagcagattac"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
ORIGIN
        1 ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA
       61 TCAATCTAAA GTATATATGA GTAAACTTGG TCTGACAGCT CGAGGCTTGG ATTCTCACCA
      121 ATAAAAAACG CCCGGCGGCA ACCGAGCGTT CTGAACAAAT CCAGATGGAG TTCTGAGGTC
      181 ATTACTGGAT CTATCAACAG GAGTCCAAGC GAGCTCGATA TCAAATTACG CCCCGCCCTG
      241 CCACTCATCG CAGTACTGTT GTAATTCATT AAGCATTCTG CCGACATGGA AGCCATCACA
      301 AACGGCATGA TGAACCTGAA TCGCCAGCGG CATCAGCACC TTGTCGCCTT GCGTATAATA
      361 TTTGCCCATG GTGAAAACGG GGGCGAAGAA GTTGTCCATA TTGGCCACGT TTAAATCAAA
      421 ACTGGTGAAA CTCACCCAGG GATTGGCTGA GACGAAAAAC ATATTCTCAA TAAACCCTTT
      481 AGGGAAATAG GCCAGGTTTT CACCGTAACA CGCCACATCT TGCGAATATA TGTGTAGAAA
      541 CTGCCGGAAA TCGTCGTGGT ATTCACTCCA GAGCGATGAA AACGTTTCAG TTTGCTCATG
      601 GAAAACGGTG TAACAAGGGT GAACACTATC CCATATCACC AGCTCACCGT CTTTCATTGC
      661 CATACGAAAT TCCGGATGAG CATTCATCAG GCGGGCAAGA ATGTGAATAA AGGCCGGATA
      721 AAACTTGTGC TTATTTTTCT TTACGGTCTT TAAAAAGGCC GTAATATCCA GCTGAACGGT
      781 CTGGTTATAG GTACATTGAG CAACTGACTG AAATGCCTCA AAATGTTCTT TACGATGCCA
      841 TTGGGATATA TCAACGGTGG TATATCCAGT GATTTTTTTC TCCATTTTAG CTTCCTTAGC
      901 TCCTGAAAAT CTCGATAACT CAAAAAATAC GCCCGGTAGT GATCTTATTT CATTATGGTG
      961 AAAGTTGGAA CCTCTTACGT GCCCGATCAA CTCGAGTGCC ACCTGACGTC TAAGAAACCA
     1021 TTATTATCAT GACATTAACC TATAAAAATA GGCGTATCAC GAGGCAGAAT TTCAGATAAA
     1081 AAAAATCCTT AGCTTTCGCT AAGGATGATT TCTGGAATTC GGTCTCAcca tgacaaattc
     1141 tcaagcgctt tcacaaccaa ttgcctcctc taacgttcat gataacttca tgaataatga
     1201 aatcacggct agtaaaattg atgatggtaa taattcaaaa ccactgtcac ctggttggac
     1261 ggaccaaact gcgtataacg cgtttggaat cactacaggg atgtttaata ccactacaat
     1321 ggatgatgta tataactatc tattcgatga tgaagatacc ccaccaaaac caaaaaaaga
     1381 gggtggcagt ggagggggcg gaagcggggg aatgCGAGAC CCTGCAGTCC GGCAAAAAAG
     1441 GGCAAGGTGT CACCACCCTG CCCTTTTTCT TTAAAACCGA AAAGATTACT TCGCGTTATG
     1501 CAGGCTTCCT CGCTCACTGA CTCGCTGCGC TCGGTCGTTC GGCTGCGGCG AGCGGTATCA
     1561 GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC
     1621 ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT
     1681 TTCCACAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG
     1741 CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC
     1801 TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC
     1861 GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC
     1921 AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC
     1981 TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT
     2041 AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT
     2101 AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC
     2161 TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT
     2221 TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG
     2281 ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC
//