LOCUS       pEPQD0CM0705_(L0_phiC31 3945 bp ds-DNA     circular     06-OCT-2020
DEFINITION  .
FEATURES             Location/Qualifiers
     primer          166..188
                     /label="pUAP/DP2-F"
                     /note="sequence: ggcgtatcacgaggcagaatttc"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     CDS             268..2106
                     /label="Translation 268-2106"
     misc_feature    271..2082
                     /label="phiC31 Integrase (UniProt Q9T221), N benthamiana codon optimized, BpiI domesticated  "
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    271..2103
                     /label="phiC31 integrase plus NLS"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     primer          complement(642..663)
                     /label="QMD639_phiC31_I141A_R"
                     /note="sequence: tatGAAGACgtGTGCaaggtccatcacattaccctgc"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     primer          666..688
                     /label="QMD638_phiC31_I141A_F"
                     /note="sequence: tatGAAGACggGCACaccttattatgcgactcgacg"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     primer          complement(1516..1536)
                     /label="QMD641_phiC31_I432A_R"
                     /note="sequence: tatGAAGACtcTTGCcttgttgaaaatacgttcggc"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer          1540..1558
                     /label="QMD640_phiC31_I432A_F"
                     /note="sequence: tatGAAGACagGCAAgacacgctgaaggggatg"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    2083..2103
                     /label="SV40 NLS (PKKKRKV)"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     primer          complement(2157..2177)
                     /label="pUAP/DP2-R"
                     /note="sequence: ggtggtgacaccttgcccttt"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     primer          complement(2204..2224)
                     /label="QMD499_pUAP/DP2-R2"
                     /note="sequence: CCTGCATAACGCGAAGTAATC"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     primer          complement(2304..2329)
                     /label="211or (L0 FWD)"
                     /note="sequence: CGTTATCCCCTGATTCTGTGGATAAC"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    complement(2400..2988)
                     /label="origin of replication (derivative of pUC/pBR322/pMB1/colE1), T-->C at 3' end, pUAP vectors"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     primer_bind     complement(2445..2463)
                     /label="QMD062 primer"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     primer          complement(2445..2463)
                     /label="QMD062_pJL1_outside(BR322)_F"
                     /note="sequence: tcgccacctctgacttgag"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer_bind     complement(2480..2499)
                     /label="pBR322ori-F"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     primer          2945..2966
                     /label="QMD540_pBR322_seq_R"
                     /note="sequence: ttgtttgcaagcagcagattac"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    complement(3286..3945)
                     /label="Chloramphenicol acetyltransferase (CAT) - CamR"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     primer          3860..3879
                     /label="QMD463_CATseq_R"
                     /note="sequence: gcaactgactgaaatgcctc"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
ORIGIN
        1 tttagcttcc ttagctcctg aaaatctcga taactcaaaa aatacgcccg gtagtgatct
       61 tatttcatta tggtgaaagt tggaacctct tacgtgcccg atcaactcga gtgccacctg
      121 acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc
      181 agaatttcag ataaaaaaaa tccttagctt tcgctaagga tgatttctgg aattcgcggc
      241 cgcttctaga gcgatgagtg gtctcgaatg gacacgtatg ccggcgctta tgatagacaa
      301 tcaagagaaa gagagaactc ttctgccgcc tctccagcta cacagagatc tgccaacgag
      361 gataaagctg ctgatttaca aagagaggta gagagagatg ggggtaggtt tagatttgtg
      421 ggtcatttct ctgaggctcc tggtacatca gcctttggta ctgctgaacg gcctgaattt
      481 gaaagaatat taaatgagtg tcgggccggt cgactaaata tgataatagt ttatgatgta
      541 tctaggttca gtagactcaa agttatggat gcaattccga tcgtgagcga gctacttgca
      601 ctcggtgtta ctatcgtaag tacacaagaa ggtgtattca ggcagggtaa tgtgatggac
      661 cttatacacc ttattatgcg actcgacgct agccataaag agtcatcact gaagagtgct
      721 aaaatcttgg acaccaagaa cctgcaaagg gagcttggag gctatgtagg aggaaaagct
      781 ccgtatggtt tcgaactcgt gagtgagaca aaagagatta ccagaaatgg taggatggtg
      841 aacgtggtta ttaataagct agctcatagc acaacccctc tcacagggcc ttttgagttc
      901 gaacctgacg ttattagatg gtggtggaga gagatcaaaa ctcacaaaca tcttccattt
      961 aagccaggta gccaggctgc tatccaccct ggatctatca ctgggctttg taaaaggatg
     1021 gatgctgacg cagtgccaac tcgaggggaa acaatcggta agaaAACTgc atcttccgct
     1081 tgggatcccg ctacagttat gagaatcctt cgggatccgc gtattgctgg attcgccgca
     1141 gaagtcattt ataagaaaaa acctgatggt actcctacta caaaaatcga aggttatagg
     1201 atccaaaggg atcctattac tttgcgtcca gttgaattag actgtggacc gatcatcgaa
     1261 cctgccgagt ggtacgagtt gcaggcctgg cttgatggta gagggcgtgg aaagggttta
     1321 tctcggggac aggcaatttt gtcagcaatg gataagcttt attgtgagtg tggggctgtt
     1381 atgacatcaa aacgtggaga agagtcaatc aaggattctt acaggtgcag acgtcgtaaa
     1441 gtggtggacc cttctgctcc aggacagcat gagggtacat gcaacgtttc tatggctgct
     1501 cttgataaat ttgtggccga acgtattttc aacaagatta gacacgctga aggggatgag
     1561 gaaactttag ctcttctttg ggaggctgcc cggcgttttg gtaagctaac agaggcacca
     1621 gaaaaaagtg gcgaaagagc taatttggta gccgaaagag cagacgcctt gaatgcattg
     1681 gaagaacttt acgaagatag ggctgccgga gcatatgatg gaccagttgg tcggaagcat
     1741 ttcaggaagc aacaagctgc actaactttg agacagcaag gcgcagagga gcgactcgca
     1801 gagcttgagg ccgccgaggc cccaaagctt ccactcgatc aatggtttcc tgaggatgcc
     1861 gacgctgatc ctacaggtcc aaagagctgg tggggaagag ctagcgtaga cgataagaga
     1921 gtgtttgtgg gtctatttgt tgacaagatt gtagttacta agtccactac gggtcgagga
     1981 cagggcactc ccatcgaaaa gagagccagt attacctggg caaagcctcc aaccgatgat
     2041 gacGAGGacg atgcacaaga tggtaccgag gatgtggcag caccaaaaaa aaagaggaag
     2101 gtgtaagctt tgagaccact catcgcgact agtagcggcc gctgcagtcc ggcaaaaaag
     2161 ggcaaggtgt caccaccctg ccctttttct ttaaaaccga aaagattact tcgcgttatg
     2221 caggcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     2281 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     2341 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     2401 ttccacaggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     2461 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     2521 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     2581 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     2641 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     2701 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     2761 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     2821 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
     2881 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     2941 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     3001 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     3061 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
     3121 tcaatctaaa gtatatatga gtaaacttgg tctgacagct cgaggcttgg attctcacca
     3181 ataaaaaacg cccggcggca accgagcgtt ctgaacaaat ccagatggag ttctgaggtc
     3241 attactggat ctatcaacag gagtccaagc gagctcgata tcaaattacg ccccgccctg
     3301 ccactcgtcg cagtactgtt gtaattcatt aagcattctg ccgacatgga agccatcaca
     3361 aacggcatga tgaacctgaa tcgccagcgg catcagcacc ttgtcgcctt gcgtataata
     3421 tttgcccatg gtgaaaacgg gggcgaagaa gttgtccata ttggccacgt ttaaatcaaa
     3481 actggtgaaa ctcacccagg gattggctga tacgaaaaac atattctcaa taaacccttt
     3541 agggaaatag gccaggtttt caccgtaaca cgccacatct tgcgaatata tgtgtagaaa
     3601 ctgccggaaa tcgtcgtggt attcactcca gagtgatgaa aacgtttcag tttgctcatg
     3661 gaaaacggtg taacaagggt gaacactatc ccatatcacc agctcaccgt ctttcattgc
     3721 catacgaaat tccggatgag cattcatcag gcgggcaaga atgtgaataa aggccggata
     3781 aaacttgtgc ttatttttct ttacggtctt taaaaaggcc gtaatatcca gctgaacggt
     3841 ctggttatag gtacattgag caactgactg aaatgcctca aaatgttctt tacgatgcca
     3901 ttgggatata tcaacggtgg tatatccagt gatttttttc tccat
//