LOCUS       pEPKK0CM0480_(pUAP_Op35 2303 bp ds-DNA     circular     06-OCT-2020
DEFINITION  .
FEATURES             Location/Qualifiers
     primer          complement(443..463)
                     /label="pUAP1_LV0 REV"
                     /note="sequence: ACTGCAGGTCTCCAGACCGAATTCCAGAAATCATCC"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     primer          464..481
                     /label="2x OpattB_min35S (CCAT-TACT) FWD"
                     /note="sequence: ACTGCAGGTCTCGGTCTCACCATggtgcggtgc"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    472..475
                     /label="additional sites for pcr"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    476..584
                     /label="OpattB"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    507..530
                     /label="repeat seq"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    566..589
                     /label="repeat"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    589..637
                     /label="min 35S"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    645..665
                     /label="part of TMV 5'UTR"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    672..697
                     /label="part of TMV 5'UTR"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    714..717
                     /label="TACT"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     primer          718..735
                     /label="pUAP1_LV0 FWD"
                     /note="sequence: ACTGCAGGTCTCCTaCGAGACCCTGCAGTCCGG"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     Replication_ori complement(983..1571)
                     /label="ori"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     Terminator      1754..1848
                     /label="lambda t0 terminator"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     CDS             complement(join(1869..2303,1..225))
                     /label="CmR"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
ORIGIN
        1 CATACGAAAT TCCGGATGAG CATTCATCAG GCGGGCAAGA ATGTGAATAA AGGCCGGATA
       61 AAACTTGTGC TTATTTTTCT TTACGGTCTT TAAAAAGGCC GTAATATCCA GCTGAACGGT
      121 CTGGTTATAG GTACATTGAG CAACTGACTG AAATGCCTCA AAATGTTCTT TACGATGCCA
      181 TTGGGATATA TCAACGGTGG TATATCCAGT GATTTTTTTC TCCATTTTAG CTTCCTTAGC
      241 TCCTGAAAAT CTCGATAACT CAAAAAATAC GCCCGGTAGT GATCTTATTT CATTATGGTG
      301 AAAGTTGGAA CCTCTTACGT GCCCGATCAA CTCGAGTGCC ACCTGACGTC TAAGAAACCA
      361 TTATTATCAT GACATTAACC TATAAAAATA GGCGTATCAC GAGGCAGAAT TTCAGATAAA
      421 AAAAATCCTT AGCTTTCGCT AAGGATGATT TCTGGAATTC GGTCTCACCA Tggtgcggtg
      481 cgggtgccag ggcgtgccct tgggctcccc gggcgcgtac tccactcccc gagccggtgc
      541 gggtgccagg gcgtgccctt gggctccccg ggcgcgtact ccactcccct tcgcaagacc
      601 cttcctctat ataaggaagt tcatttcatt tggagaggac tccggtattt ttacaacaat
      661 taccacaaca aaacaaacaa caaacaacat tacaatttac tattctagtc gaaTACTCGA
      721 GACCCTGCAG TCCGGCAAAA AAGGGCAAGG TGTCACCACC CTGCCCTTTT TCTTTAAAAC
      781 CGAAAAGATT ACTTCGCGTT ATGCAGGCTT CCTCGCTCAC TGACTCGCTG CGCTCGGTCG
      841 TTCGGCTGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT
      901 CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA
      961 AAAAGGCCGC GTTGCTGGCG TTTTTCCACA GGCTCCGCCC CCCTGACGAG CATCACAAAA
     1021 ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC
     1081 CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT
     1141 CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA
     1201 GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG
     1261 ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT
     1321 CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA
     1381 CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT
     1441 GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC
     1501 AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA
     1561 AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA
     1621 ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT
     1681 TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA
     1741 GCTCGAGGCT TGGATTCTCA CCAATAAAAA ACGCCCGGCG GCAACCGAGC GTTCTGAACA
     1801 AATCCAGATG GAGTTCTGAG GTCATTACTG GATCTATCAA CAGGAGTCCA AGCGAGCTCG
     1861 ATATCAAATT ACGCCCCGCC CTGCCACTCA TCGCAGTACT GTTGTAATTC ATTAAGCATT
     1921 CTGCCGACAT GGAAGCCATC ACAAACGGCA TGATGAACCT GAATCGCCAG CGGCATCAGC
     1981 ACCTTGTCGC CTTGCGTATA ATATTTGCCC ATGGTGAAAA CGGGGGCGAA GAAGTTGTCC
     2041 ATATTGGCCA CGTTTAAATC AAAACTGGTG AAACTCACCC AGGGATTGGC TGAGACGAAA
     2101 AACATATTCT CAATAAACCC TTTAGGGAAA TAGGCCAGGT TTTCACCGTA ACACGCCACA
     2161 TCTTGCGAAT ATATGTGTAG AAACTGCCGG AAATCGTCGT GGTATTCACT CCAGAGCGAT
     2221 GAAAACGTTT CAGTTTGCTC ATGGAAAACG GTGTAACAAG GGTGAACACT ATCCCATATC
     2281 ACCAGCTCAC CGTCTTTCAT TGC
//