LOCUS       pEPQD1CB0107_(P1__35Ssh 7314 bp ds-DNA     circular     13-APR-2019
DEFINITION  .
FEATURES             Location/Qualifiers
     Misc._feature   30..177
                     /label="LB"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     Promoter        233..658
                     /label="CaMV 35S promoter short"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_feature    236..561
                     /label="CaMV 35S promoter repeat unit"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     5'_UTR          663..745
                     /label="TMV omega"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     Transit_peptide 746..921
                     /label="chloroplast targeting signal"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    926..2962
                     /label="CrDXS2 (no transit peptide, domesticated, 1 BsaI site removed) amplified from NS-I-132A2 V2, similar to DQ848672.1 but contains R414K, T533A, A592T"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     Terminator      2967..3170
                     /label="35S ter"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     Misc._feature   complement(3202..3329)
                     /label="RB short"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     Origin          complement(3353..4834)
                     /label="RK2"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     Origin          4845..5634
                     /label="pUC Ori"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     CDS             complement(5642..6499)
                     /label="AmpR"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     origin          complement(6697..7314)
                     /label="OriV"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
ORIGIN
        1 TGAGCGTCGC AAAGGAGATC CTGATCTGAC TGATGGGCTG CCTGTATCGA GTGGTGATTT
       61 TGTGCCGAGC TGCCGGTCGG GGAGCTGTTG GCTGGCTGGT GGCAGGATAT ATTGTGGTGT
      121 AAACAAATTG ACGCTTAGAC AACTTAATAA CACATTGCGG ACGTTTTTAA TGTACTGGGG
      181 TGGATGCAGT GGGCCCCACT CTGTGAAGAC AATGCCGAAT TCGGATCCGG AGGTCAACAT
      241 GGTGGAGCAC GACACTCTGG TCTACTCCAA AAATGTCAAA GATACAGTCT CAGAAGATCA
      301 AAGGGCTATT GAGACTTTTC AACAAAGGAT AATTTCGGGA AACCTCCTCG GATTCCATTG
      361 CCCAGCTATC TGTCACTTCA TCGAAAGGAC AGTAGAAAAG GAAGGTGGCT CCTACAAATG
      421 CCATCATTGC GATAAAGGAA AGGCTATCAT TCAAGATCTC TCTGCCGACA GTGGTCCCAA
      481 AGATGGACCC CCACCCACGA GGAGCATCGT GGAAAAAGAA GAGGTTCCAA CCACGTCTAC
      541 AAAGCAAGTG GATTGATGTG ACATCTCCAC TGACGTAAGG GATGACGCAC AATCCCACTA
      601 TCCTTCGCAA GACCCTTCCT CTATATAAGG AAGTTCATTT CATTTGGAGA GGACACGCTA
      661 CTGTATAAGA GCTCATTTTT ACAACAATTA CCAACAACAA CAAACAACAA ACAACATTAC
      721 AATTACATTT ACAATTATCG ATAACATGGC TTCTTCTATG CTTTCTTCTG CTGCTGTTGT
      781 TGCTACTCGT GCTAGTGCTG CTCAAGCTAG TATGGTTGCT CCTTTTACTG GACTTAAGTC
      841 TGCTGCTTCT TTTCCTGTTA CTAGAAAGCA AAACAACCTT GATATTACTT CTATTGCTAG
      901 TAACGGAGGA AGAGTTCGAG CAATGGCTGC TGCTCATGGG CATGCTCATA ATGTTGATGA
      961 AAATTACCAA GTCCTAGTGA AGAAGCAGCC TTTGAATTTT TCAGGAGATA AGCCTTTTAC
     1021 TCCTATTTTG GATACCATCA ACTACCCTAC CCATATGAAG AATCTCTCTC TTCAGGAGCT
     1081 TCAACAATTA GCTGACGAAT TAAGGGAGGA GATAGTGTAT TCTGTGGCCA AAACGGGTGG
     1141 TCACTTAAGT TCCAGCCTAG GTGTGGCCGA GCTCACTGTG GCACTCCACC ACGTGTTCGA
     1201 AACCCCAAAT GATAAAATCA TATGGGATGT TGGCCACCAG ACATATGGGC ATAAGATTCT
     1261 GACAGGGAGA AGGTCAAAGA TGCATACCAT GAGACAAACT TGCGGACTTG CAGGTTTCCC
     1321 CAAAAGAGAT GAAAGTGCCC ATGATGCATT TGGAGTTGGC CATAGTTCCA CCAGCATTTC
     1381 TGCCGGTTTA GGAATGGCAA TAGGACGAGA TTTATTAGGT AAGAAAAATC ATGTAATAAC
     1441 GGTAATAGGA GATGGAGCTA TGACAGCCGG ACAAGCATAT GAAGCCATGA ATAATGCTGG
     1501 ATATCTTGAT TCTAATCTCA TAATCATATT GAATGATAAC AAACAAGTTT CTCTCCCGAC
     1561 TGCCACAATT GATGGTCCTG CACCTCCTAC TGGTGCTCTA AGCAATGCCT TAACTAGGCT
     1621 TCAATCTAGT AGAAAATTTC GACTACTTCG GGAGGCTGCA AAAGGTGTGA CAAAGCAATT
     1681 AGGGGGACAA GTTCATGAAA TGGCAGCTAA AGTGGATACA TATATGAGAG GGATGAATAG
     1741 CCAAAGGGCT TCCTTATTTG AAGATTTAGG AATGTATTAT ATTGGTCCAC TTGATGGTCA
     1801 CAATATGGAA GATCTTGTTG ATATTTTAGA GAAAGTAAAA TCTGTTCCTG CAACGGGCCC
     1861 TGTTCTTATT CATATTATTA CTGAGAAAGG AAAAGGATAT CCTCCTGCTG AAGCTGCCGC
     1921 CGACAAAATG CATGGGGTGG TGAAATTTGA GCCAATGACA GGAAAACAAT TCAAGTCAAA
     1981 GTCAAAAACA AAGTCATATA CAAATTATTT TGCTGAGAGT TTAATAAGTG AAGCTAGGGA
     2041 AGATGATAAG ATTATTGCCA TTCATGCTGC TATGGGAGGA GGAACAGGAC TCAATTTATT
     2101 CCAGAAACAC TTTCCTGAAC GATGCTTTGA TGTTGGGATA GCTGAGCAAC ATGCTGTCAC
     2161 TTTTGCTGCT GGCTTAGCTT CTGAAGGACT CAAGCCTTTT TGTGCTATTT ACTCATCTTT
     2221 CTTACAAAGA GGTTATGATC AGGTGGTTCA TGATGTGGAC CTTCAAAAAA TTCCAGTAAG
     2281 GTTTGCTATG GATAGAGCAG GGCTGGTGGG TGCAGATGGA CCCACTCATT GCGGTGCATT
     2341 TGACACAACT TTTATGGCTT GTTTGCCTAA TATGGTGGTC ATGGCTCCTT CAGATGAAAC
     2401 CGAATTGATG CACATGGTTG CCACAGCAGC TGCCATTGAT GATAGGCCTA GTTGCTTTCG
     2461 ATATCCCAGA GGAAATGGCA TTGGTGCAAT TCTTCCACCA AACAACAAAG GAACTCCATT
     2521 ACAGATTGGT AAAGGAAGAA TATTGTTGGA GGGAACAAGA GTTGCCATTC TTGGGTATGG
     2581 AGCAATGGTG CAAAACTGTT TAATGGCTGC TCAGCTTCTT GAAATTAATG GAATATCAGC
     2641 TACAGTTGTT GATGCCCGAT TTTGCAAACC TCTTGATGGA GATTTGATTA GAAAATTGGC
     2701 TCAACAACAT GAAGTCCTTA TCACTGTTGA AGAAGGATCC ATTGGTGGTT TTGGTTCCCA
     2761 TGTGGCACAG TTCTTGGCTT TGAATGGATT GCTTGATGGA AATTTAAAGT GGAGGGCAAT
     2821 GATGCTTCCT GACAGATATA TTGATCATGG AGCTCAAAAT GACCAAATTG AAGAGGCTGG
     2881 CCTAACTCCA AAGCATATTG CTGGCACTGT GTTTTCTCTA CTCAGCAAAA ACAAGAATAC
     2941 TCTTCAAGTT CTCCAGTTTT AGGCTTCTCT AGCTAGAGTC GATCGACAAG CTCGAGTTTC
     3001 TCCATAATAA TGTGTGAGTA GTTCCCAGAT AAGGGAATTA GGGTTCCTAT AGGGTTTCGC
     3061 TCATGTGTTG AGCATATAAG AAACCCTTAG TATGTATTTG TATTTGTAAA ATACTTCTAT
     3121 CAATAAAATT TCTAATTCCT AAAACCAAAA TCCAGTACTA AAATCCAGAT CGCTGCAATT
     3181 GTCTTCTGCA CGAAGTGGTT TAAACTATCA GTGTTTGACA GGATATATTG GCGGGTAAAC
     3241 CTAAGAGAAA AGAGCGTTTA TTAGAATAAT CGGATATTTA AAAGGGCGTG AAAAGGTTTA
     3301 TCCGTTCGTC CATTTGTATG TGCATGCCAA CCACAGGGTT CCCCAGATCA GGCGCTGGCT
     3361 GCTGAACCCC CAGCCGGAAC TGACCCCACA AGGCCCTAGC GTTTGCAATG CACCAGGTCA
     3421 TCATTGACCC AGGCGTGTTC CACCAGGCCG CTGCCTCGCA ACTCTTCGCA GGCTTCGCCG
     3481 ACCTGCTCGC GCCACTTCTT CACGCGGGTG GAATCCGATC CGCACATGAG GCGGAAGGTT
     3541 TCCAGCTTGA GCGGGTACGG CTCCCGGTGC GAGCTGAAAT AGTCGAACAT CCGTCGGGCC
     3601 GTCGGCGACA GCTTGCGGTA CTTCTCCCAT ATGAATTTCG TGTAGTGGTC GCCAGCAAAC
     3661 AGCACGACGA TTTCCTCGTC GATCAGGACC TGGCAACGGG ACGTTTTCTT GCCACGGTCC
     3721 AGGACGCGGA AGCGGTGCAG CAGCGACACC GATTCCAGGT GCCCAACGCG GTCGGACGTG
     3781 AAGCCCATCG CCGTCGCCTG TAGGCGCGAC AGGCATTCCT CGGCCTTCGT GTAATACCGG
     3841 CCATTGATCG ACCAGCCCAG GTCCTGGCAA AGCTCGTAGA ACGTGAAGGT GATCGGCTCG
     3901 CCGATAGGGG TGCGCTTCGC GTACTCCAAC ACCTGCTGCC ACACCAGTTC GTCATCGTCG
     3961 GCCCGCAGCT CGACGCCGGT GTAGGTGATC TTCACGTCCT TGTTGACGTG GAAAATGACC
     4021 TTGTTTTGCA GCGCCTCGCG CGGGATTTTC TTGTTGCGCG TGGTGAACAG GGCAGAGCGG
     4081 GCCGTGTCGT TTGGCATCGC TCGCATCGTG TCCGGCCACG GCGCAATATC GAACAAGGAA
     4141 AGCTGCATTT CCTTGATCTG CTGCTTCGTG TGTTTCAGCA ACGCGGCCTG CTTGGCCTCG
     4201 CTGACCTGTT TTGCCAGGTC CTCGCCGGCG GTTTTTCGCT TCTTGGTCGT CATAGTTCCT
     4261 CGCGTGTCGA TGGTCATCGA CTTCGCCAAA CCTGCCGCCT CCTGTTCAAG ACGACGCGAA
     4321 CGCTCCACGG CGGCCGATGG CGCGGGCAGG GCAGGGGGAG CCAGTTGCAC GCTGTCGCGC
     4381 TCGATCTTGG CCGTAGCTTG CTGGACCATC GAGCCGACGG ACTGGAAGGT TTCGCGGGGC
     4441 GCACGCATGA CGGTGCGGCT TGCGATGGTT TCGGCATCCT CGGCGGAAAA CCCCGCGTCG
     4501 ATCAGTTCTT GCCTGTATGC CTTCCGGTCA AACGTCCGAT TCATTCACCC TCCTTGCGGG
     4561 ATTGCCCCGA CTCACGCCGG GGCAATGTGC CCTTATTCCT GATTTGACCC GCCTGGTGCC
     4621 TTGGTGTCCA GATAATCCAC CTTATCGGCA ATGAAGTCGG TCCCGTAGAC CGTCTGGCCG
     4681 TCCTTCTCGT ACTTGGTATT CCGAATCTTG CCCTGCACGA ATACCAGCGA CCCCTTGCCC
     4741 AAATACTTGC CGTGGGCCTC GGCCTGAGAG CCAAAACACT TGATGCGGAA GAAGTCGGTG
     4801 CGCTCCTGCT TGTCGCCGGC ATCGTTGCGC CACATCTAGG ATCTGCCAGG AACCGTAAAA
     4861 AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC
     4921 GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC
     4981 CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG
     5041 CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT
     5101 CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC
     5161 GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC
     5221 CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG
     5281 AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG
     5341 CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA
     5401 CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG
     5461 GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT
     5521 CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA
     5581 ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA GTAAACTTGG TCTGACAGTT
     5641 ACCAATGCTT AATCAGTGAG GCACCTATCT CAGCGATCTG TCTATTTCGT TCATCCATAG
     5701 TTGCCTGACT CCCCGTCGTG TAGATAACTA CGATACGGGA GGGCTTACCA TCTGGCCCCA
     5761 GTGCTGCAAT GATACCGCGA GAACCACGCT CACCGGCTCC AGATTTATCA GCAATAAACC
     5821 AGCCAGCCGG AAGGGCCGAG CGCAGAAGTG GTCCTGCAAC TTTATCCGCC TCCATCCAGT
     5881 CTATTAATTG TTGCCGGGAA GCTAGAGTAA GTAGTTCGCC AGTTAATAGT TTGCGCAACG
     5941 TTGTTGCCAT TGCTACAGGC ATCGTGGTGT CACGCTCGTC GTTTGGTATG GCTTCATTCA
     6001 GCTCCGGTTC CCAACGATCA AGGCGAGTTA CATGATCCCC CATGTTGTGC AAAAAAGCGG
     6061 TTAGCTCCTT CGGTCCTCCG ATCGTTGTCA GAAGTAAGTT GGCCGCAGTG TTATCACTCA
     6121 TGGTTATGGC AGCACTGCAT AATTCTCTTA CTGTCATGCC ATCCGTAAGA TGCTTTTCTG
     6181 TGACTGGTGA GTACTCAACC AAGTCATTCT GAGAATAGTG TATGCGGCGA CCGAGTTGCT
     6241 CTTGCCCGGC GTCAATACGG GATAATACCG CGCCACATAG CAGAACTTTA AAAGTGCTCA
     6301 TCATTGGAAA ACGTTCTTCG GGGCGAAAAC TCTCAAGGAT CTTACCGCTG TTGAGATCCA
     6361 GTTCGATGTA ACCCACTCGT GCACCCAACT GATCTTCAGC ATCTTTTACT TTCACCAGCG
     6421 TTTCTGGGTG AGCAAAAACA GGAAGGCAAA ATGCCGCAAA AAAGGGAATA AGGGCGACAC
     6481 GGAAATGTTG AATACTCATA CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAAGGTT
     6541 ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC
     6601 CGCGCACGAA TTGGCCAGCG CTGCCATTTT TGGGGTGAGG CCGTTCGCGG CCGAGGGGCG
     6661 CAGCCCCTGG GGGGATGGGA GGCCCGCGTT AGCGGGCCGG GAGGGTTCGA GAAGGGGGGG
     6721 CACCCCCCTT CGGCGTGCGC GGTCACGCGC ACAGGGCGCA GCCCTGGTTA AAAACAAGGT
     6781 TTATAAATAT TGGTTTAAAA GCAGGTTAAA AGACAGGTTA GCGGTGGCCG AAAAACGGGC
     6841 GGAAACCCTT GCAAATGCTG GATTTTCTGC CTGTGGACAG CCCCTCAAAT GTCAATAGGT
     6901 GCGCCCCTCA TCTGTCAGCA CTCTGCCCCT CAAGTGTCAA GGATCGCGCC CCTCATCTGT
     6961 CAGTAGTCGC GCCCCTCAAG TGTCAATACC GCAGGGCACT TATCCCCAGG CTTGTCCACA
     7021 TCATCTGTGG GAAACTCGCG TAAAATCAGG CGTTTTCGCC GATTTGCGAG GCTGGCCAGC
     7081 TCCACGTCGC CGGCCGAAAT CGAGCCTGCC CCTCATCTGT CAACGCCGCG CCGGGTGAGT
     7141 CGGCCCCTCA AGTGTCAACG TCCGCCCCTC ATCTGTCAGT GAGGGCCAAG TTTTCCGCGA
     7201 GGTATCCACA ACGCCGGCGG CCGCGGTGTC TCGCACACGG CTTCGACGGC GTTTCTGGCG
     7261 CGTTTGCAGG GCCATAGACG GCCGCCAGCC CAGCGGCGAG GGCAACCAGC CCGG
//