LOCUS       pEPQD1CB0108_(P2_35Ssho 6051 bp ds-DNA     circular     13-APR-2019
DEFINITION  .
FEATURES             Location/Qualifiers
     primer          88..111
                     /label="214or (L1, REV, LB)"
                     /note="sequence: CTGGTGGCAGGATATATTGTGGTG"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    complement(92..116)
                     /label="LB T-DNA repeat"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     Promoter        225..650
                     /label="CaMV 35S promoter short"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     5'_UTR          667..728
                     /label="TMV Omega"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    741..1688
                     /label="PaGPPS1 (67aa truncated, domesticed, 1 BpiI and 1 SapI site removed) GQ369788.1 genbank  D2IT07 ACZ57571.1 Picea abies (Norway spruce)"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     Terminator      1696..1899
                     /label="35S ter"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    complement(1945..1969)
                     /label="RB T-DNA repeat"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     primer          complement(2045..2070)
                     /label="213or (L1, FWD, RB)"
                     /note="sequence: GAACCCTGTGGTTGGCATGCACATAC"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     CDS             complement(2125..3273)
                     /label="trfA"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     rep_origin      complement(3609..4197)
                     /label="ori"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     primer_bind     complement(3689..3708)
                     /label="pBR322ori-F"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     CDS             complement(4368..5228)
                     /label="AmpR"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     primer_bind     4991..5010
                     /label="Amp-R"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     promoter        complement(5229..5333)
                     /label="AmpR promoter"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     rep_origin      complement(5420..6051)
                     /label="oriV"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
ORIGIN
        1 gcaaaggaga tcctgatctg actgatgggc tgcctgtatc gagtggtgat tttgtgccga
       61 gctgccggtc ggggagctgt tggctggctg gtggcaggat atattgtggt gtaaacaaat
      121 tgacgcttag acaacttaat aacacattgc ggacgttttt aatgtactgg ggtggatgca
      181 gtgggcccca ctctgtgaag acaagcaaga attcaagctt ggagGTCAAC ATGGTGGAGC
      241 ACGACACTCT GGTCTACTCC AAAAATGTCA AAGATACAGT CTCAGAAGAT CAAAGGGCTA
      301 TTGAGACTTT TCAACAAAGG ATAATTTCGG GAAACCTCCT CGGATTCCAT TGCCCAGCTA
      361 TCTGTCACTT CATCGAAAGG ACAGTAGAAA AGGAAGGTGG CTCCTACAAA TGCCATCATT
      421 GCGATAAAGG AAAGGCTATC ATTCAAGATC TCTCTGCCGA CAGTGGTCCC AAAGATGGAC
      481 CCCCACCCAC GAGGAGCATC GTGGAAAAAG AAGAGGTTCC AACCACGTCT ACAAAGCAAG
      541 TGGATTGATG TGACATCTCC ACTGACGTAA GGGATGACGC ACAATCCCAC TATCCTTCGC
      601 AAGACCCTTC CTCTATATAA GGAAGTTCAT TTCATTTGGA GAGGACACGC TCGAGTATAA
      661 GAGCTCATTT TTACAACAAT TACCAACAAC AACAAACAAC AAACAACATT ACAATTACAT
      721 TTACAATTAT CGATACAATG TGCTCAAACA CAAATGCCCA GTTGGCCAAT TTGTCTGAAC
      781 AAGTAAAGGG GAAGGTCACT GAATTTGATT TCAAGGAGTA CATGCGTTCC AAGGCAATGT
      841 CAGTGAATGA GGCACTGGAC AGGGCTGTCC CACTTCGTTA TCCTGAAAAA ATACATGAAG
      901 CTATGAGGTA TTCTCTTCTA GCAGGAGGGA AGCGAGTTCG TCCTATTCTG TGCATTGCAG
      961 CATGTGAGCT TGTGGGAGGG AGCGAGGAGC TCGCCATGCC AACTGCCTGT GCAATGGAAA
     1021 TTATCCACAC AATGTCTTTG ATTCATGATG ACTTGCCTCC CATGGACAAT GATGATTTAC
     1081 GCCGAGGTAA GCCTACAAAC CACAAGGTGT TCGGTGAAGG CACTGCTGTT CTTGCGGGGG
     1141 ATGCACTTCT TTCATTTGCA TTTGAGCACA TTGCAGTGTC CACAAGCAAG ACTGTGGAGT
     1201 CTGATAGGGT TTTGAGGGTG GTATCTGAAT TGGGTAGAGC AATAGGCTCG GAAGGGGTTG
     1261 CAGGTGGGCA GGTTGCTGAT ATTACCAGTC AGGGGAATCC TTCTGTTGGC CTTGAGACTC
     1321 TGGAATGGAT TCACATTCAC AAGACTGCAG TGCTCTTGGA GTGCTCCGTT GCTAGTGGGG
     1381 CGATCATCGG TGGTGCTTCT GATGATGAGA TTGAGAGAGT TCGAAAGTAT GCCCGTTGCG
     1441 TGGGGCTTCT GTTTCAGGTT GTGGATGACA TTCTCGATGT CACGAAATCA TCAGAAGAAT
     1501 TGGGTAAGAC TGCAGCAAAG GATTTGCTTA GTGATAAGGC CACTTATCCC AAGCTCATGG
     1561 GTTTGGAGAA AGCAAAGGAG TTTGCTGATG AATTGTTGGG CAAAGCTAAG GAGGAGTTAT
     1621 CTTTCTTCAA CCCAACTAAG GCTGCACCTC TGTTGGGTCT TGCAGATTAC ATTGCACAAA
     1681 GACAGAACTG AGCTTCTCTA GCTAGAGTCG ATCGACAAGC TCGAGTTTCT CCATAATAAT
     1741 GTGTGAGTAG TTCCCAGATA AGGGAATTAG GGTTCCTATA GGGTTTCGCT CATGTGTTGA
     1801 GCATATAAGA AACCCTTAGT ATGTATTTGT ATTTGTAAAA TACTTCTATC AATAAAATTT
     1861 CTAATTCCTA AAACCAAAAT CCAGTACTAA AATCCAGATC GCTactattg tcttctgcac
     1921 gaagtggttt aaactatcag tgtttgacag gatatattgg cgggtaaacc taagagaaaa
     1981 gagcgtttat tagaataatc ggatatttaa aagggcgtga aaaggtttat ccgttcgtcc
     2041 atttgtatgt gcatgccaac cacagggttc cccagatcag gcgctggctg ctgaaccccc
     2101 agccggaact gaccccacaa ggccctagcg tttgcaatgc accaggtcat cattgaccca
     2161 ggcgtgttcc accaggccgc tgcctcgcaa ctcttcgcag gcttcgccga cctgctcgcg
     2221 ccacttcttc acgcgggtgg aatccgatcc gcacatgagg cggaaggttt ccagcttgag
     2281 cgggtacggc tcccggtgcg agctgaaata gtcgaacatc cgtcgggccg tcggcgacag
     2341 cttgcggtac ttctcccata tgaatttcgt gtagtggtcg ccagcaaaca gcacgacgat
     2401 ttcctcgtcg atcaggacct ggcaacggga cgttttcttg ccacggtcca ggacgcggaa
     2461 gcggtgcagc agcgacaccg attccaggtg cccaacgcgg tcggacgtga agcccatcgc
     2521 cgtcgcctgt aggcgcgaca ggcattcctc ggccttcgtg taataccggc cattgatcga
     2581 ccagcccagg tcctggcaaa gctcgtagaa cgtgaaggtg atcggctcgc cgataggggt
     2641 gcgcttcgcg tactccaaca cctgctgcca caccagttcg tcatcgtcgg cccgcagctc
     2701 gacgccggtg taggtgatct tcacgtcctt gttgacgtgg aaaatgacct tgttttgcag
     2761 cgcctcgcgc gggattttct tgttgcgcgt ggtgaacagg gcagagcggg ccgtgtcgtt
     2821 tggcatcgct cgcatcgtgt ccggccacgg cgcaatatcg aacaaggaaa gctgcatttc
     2881 cttgatctgc tgcttcgtgt gtttcagcaa cgcggcctgc ttggcctcgc tgacctgttt
     2941 tgccaggtcc tcgccggcgg tttttcgctt cttggtcgtc atagttcctc gcgtgtcgat
     3001 ggtcatcgac ttcgccaaac ctgccgcctc ctgttcaaga cgacgcgaac gctccacggc
     3061 ggccgatggc gcgggcaggg cagggggagc cagttgcacg ctgtcgcgct cgatcttggc
     3121 cgtagcttgc tggaccatcg agccgacgga ctggaaggtt tcgcggggcg cacgcatgac
     3181 ggtgcggctt gcgatggttt cggcatcctc ggcggaaaac cccgcgtcga tcagttcttg
     3241 cctgtatgcc ttccggtcaa acgtccgatt cattcaccct ccttgcggga ttgccccgac
     3301 tcacgccggg gcaatgtgcc cttattcctg atttgacccg cctggtgcct tggtgtccag
     3361 ataatccacc ttatcggcaa tgaagtcggt cccgtagacc gtctggccgt ccttctcgta
     3421 cttggtattc cgaatcttgc cctgcacgaa taccagcgac cccttgccca aatacttgcc
     3481 gtgggcctcg gcctgagagc caaaacactt gatgcggaag aagtcggtgc gctcctgctt
     3541 gtcgccggca tcgttgcgcc acatctagga tctgccagga accgtaaaaa ggccgcgttg
     3601 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     3661 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     3721 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     3781 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     3841 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     3901 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     3961 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     4021 tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag
     4081 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     4141 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     4201 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     4261 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     4321 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
     4381 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     4441 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     4501 ataccgcgag aaccacgctc accggctcca gatttatcag caataaacca gccagccgga
     4561 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
     4621 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
     4681 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
     4741 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
     4801 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
     4861 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
     4921 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
     4981 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
     5041 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
     5101 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
     5161 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
     5221 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
     5281 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacgaat
     5341 tggccagcgc tgccattttt ggggtgaggc cgttcgcggc cgaggggcgc agcccctggg
     5401 gggatgggag gcccgcgtta gcgggccggg agggttcgag aagggggggc accccccttc
     5461 ggcgtgcgcg gtcacgcgca cagggcgcag ccctggttaa aaacaaggtt tataaatatt
     5521 ggtttaaaag caggttaaaa gacaggttag cggtggccga aaaacgggcg gaaacccttg
     5581 caaatgctgg attttctgcc tgtggacagc ccctcaaatg tcaataggtg cgcccctcat
     5641 ctgtcagcac tctgcccctc aagtgtcaag gatcgcgccc ctcatctgtc agtagtcgcg
     5701 cccctcaagt gtcaataccg cagggcactt atccccaggc ttgtccacat catctgtggg
     5761 aaactcgcgt aaaatcaggc gttttcgccg atttgcgagg ctggccagct ccacgtcgcc
     5821 ggccgaaatc gagcctgccc ctcatctgtc aacgccgcgc cgggtgagtc ggcccctcaa
     5881 gtgtcaacgt ccgcccctca tctgtcagtg agggccaagt tttccgcgag gtatccacaa
     5941 cgccggcggc cgcggtgtct cgcacacggc ttcgacggcg tttctggcgc gtttgcaggg
     6001 ccatagacgg ccgccagccc agcggcgagg gcaaccagcc cggtgagcgt c
//