LOCUS       pEPQD1CB0118_(P6_35Ssho 6441 bp ds-DNA     circular     13-APR-2019
DEFINITION  .
FEATURES             Location/Qualifiers
     Misc._feature   30..177
                     /label="LB"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     primer          96..119
                     /label="214or (L1, REV, LB)"
                     /note="sequence: CTGGTGGCAGGATATATTGTGGTG"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    complement(100..124)
                     /label="LB T-DNA repeat"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     primer          114..134
                     /label="226or (L2, FWD, LB)"
                     /note="sequence: GTGGTGTAAACAAATTGACGC"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     Promoter        233..658
                     /label="CaMV 35S promoter short"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_feature    236..561
                     /label="CaMV 35S promoter repeat unit"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     primer          complement(562..581)
                     /label="QMD079_CaMV35Sprom_seq_R"
                     /note="sequence: CCCTTACGTCAGTGGAGATG"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     Golden_Gate_Par 659..925
                     /label="5U-TMV+SP-chloroplast"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     5'_UTR          663..745
                     /label="TMV omega"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     Transit_peptide 746..921
                     /label="chloroplast targeting signal"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     CDS             923..2089
                     /label="Translation 923-2089"
     misc_feature    926..2089
                     /label="CrISY iridoid synthase >ENA JX974564 JX974564.1 Catharanthus roseus iridoid synthase mRNA, complete cds."
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     Terminator      2094..2297
                     /label="35S ter"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     Misc._feature   complement(2329..2456)
                     /label="RB short"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    complement(2343..2367)
                     /label="RB T-DNA repeat"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     primer          complement(2411..2430)
                     /label="227or (L2, REV, RB)"
                     /note="sequence: GGATAAACCTTTTCACGCCC"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     primer          complement(2443..2468)
                     /label="213or (L1, FWD, RB)"
                     /note="sequence: GAACCCTGTGGTTGGCATGCACATAC"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     Origin          complement(2480..3961)
                     /label="RK2"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     CDS             complement(2523..3671)
                     /label="trfA"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     primer          complement(4052..4070)
                     /label="QMD062_pJL1_outside(BR322)_F"
                     /note="sequence: tcgccacctctgacttgag"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer          complement(4054..4073)
                     /label="QMD132_LETprom2_F, 61.4"
                     /note="sequence: gtttcgccacctctgacttg"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer          complement(4083..4106)
                     /label="QMD131_LETprom1_F, 61.7, -3.89 self"
                     /note="sequence: gggaaacgcctggtatctttatag"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     primer_bind     complement(4087..4106)
                     /label="pBR322ori-F"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     CDS             complement(4766..5626)
                     /label="AmpR"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer          5671..5698
                     /label="212or (L0 REV)"
                     /note="sequence: GTCTCATGAGCGGATACATATTTGAATG"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     origin          complement(5824..6441)
                     /label="OriV"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
ORIGIN
        1 TGAGCGTCGC AAAGGAGATC CTGATCTGAC TGATGGGCTG CCTGTATCGA GTGGTGATTT
       61 TGTGCCGAGC TGCCGGTCGG GGAGCTGTTG GCTGGCTGGT GGCAGGATAT ATTGTGGTGT
      121 AAACAAATTG ACGCTTAGAC AACTTAATAA CACATTGCGG ACGTTTTTAA TGTACTGGGG
      181 TGGATGCAGT GGGCCCCACT CTGTGAAGAC AATGTGGAAT TCCTCGAGGG AGGTCAACAT
      241 GGTGGAGCAC GACACTCTGG TCTACTCCAA AAATGTCAAA GATACAGTCT CAGAAGATCA
      301 AAGGGCTATT GAGACTTTTC AACAAAGGAT AATTTCGGGA AACCTCCTCG GATTCCATTG
      361 CCCAGCTATC TGTCACTTCA TCGAAAGGAC AGTAGAAAAG GAAGGTGGCT CCTACAAATG
      421 CCATCATTGC GATAAAGGAA AGGCTATCAT TCAAGATCTC TCTGCCGACA GTGGTCCCAA
      481 AGATGGACCC CCACCCACGA GGAGCATCGT GGAAAAAGAA GAGGTTCCAA CCACGTCTAC
      541 AAAGCAAGTG GATTGATGTG ACATCTCCAC TGACGTAAGG GATGACGCAC AATCCCACTA
      601 TCCTTCGCAA GACCCTTCCT CTATATAAGG AAGTTCATTT CATTTGGAGA GGACACGCTA
      661 CTGTATAAGA GCTCATTTTT ACAACAATTA CCAACAACAA CAAACAACAA ACAACATTAC
      721 AATTACATTT ACAATTATCG ATAACATGGC TTCTTCTATG CTTTCTTCTG CTGCTGTTGT
      781 TGCTACTCGT GCTAGTGCTG CTCAAGCTAG TATGGTTGCT CCTTTTACTG GACTTAAGTC
      841 TGCTGCTTCT TTTCCTGTTA CTAGAAAGCA AAACAACCTT GATATTACTT CTATTGCTAG
      901 TAACGGAGGA AGAGTTCGAG CAATGAGTTG GTGGTGGAAG AGGTCCATTG GTGCTGGAAA
      961 GAACTTACCA AACCAGAACA AAGAAAATGG AGTTTGTAAA AGCTACAAGA GTGTGGCACT
     1021 AGTAGTAGGA GTCACCGGCA TTGTTGGCAG TAGTTTAGCC GAAGTTCTAA AGCTACCAGA
     1081 CACCCCTGGA GGTCCCTGGA AGGTATACGG CGTGGCAAGG CGCCCATGCC CTGTATGGCT
     1141 AGCCAAAAAA CCAGTTGAGT ACATCCAATG TGATGTCTCA GATAATCAAG AAACAATTTC
     1201 TAAATTATCT CCACTTAAGG ACATAACTCA TATATTCTAT GTTTCTTGGA TTGGATCAGA
     1261 AGATTGCCAA ACAAATGCTA CAATGTTCAA AAACATCCTT AATTCAGTGA TTCCTAATGC
     1321 TTCAAATCTC CAACATGTGT GTCTCCAAAC AGGAATCAAA CATTATTTTG GTATTTTTGA
     1381 AGAAGGTTCT AAAGTTGTAC CACATGATTC GCCTTTTACC GAGGATTTAC CTAGGCTAAA
     1441 TGTCCCAAAT TTCTACCATG ATCTTGAAGA TATACTGTAT GAAGAAACTG GCAAGAACAA
     1501 TTTAACATGG TCTGTCCATA GACCAGCTCT GGTTTTCGGG TTTTCCCCAT GTAGTATGAT
     1561 GAACATTGTC AGTACATTAT GCGTTTACGC CACAATTTGT AAACATGAAA ATAAGGCCTT
     1621 GGTTTATCCA GGTAGTAAAA ATTCATGGAA TTGTTATGCT GATGCTGTTG ATGCAGATTT
     1681 AGTAGCCGAG CACGAAATTT GGGCAGCAGT TGATCCTAAG GCTAAAAATC AAGTATTGAA
     1741 TTGTAACAAT GGGGATGTTT TTAAATGGAA ACATATTTGG AAGAAATTAG CAGAGGAATT
     1801 TGGGATTGAA ATGGTGGGAT ATGTTGAAGG TAAAGAACAA GTTAGTTTGG CTGAGTTAAT
     1861 GAAGGATAAA GATCAAGTTT GGGATGAAAT TGTTAAGAAG AACAATTTAG TACCTACTAA
     1921 ATTGAAAGAA ATTGCTGCCT TTTGGTTTGC TGATATTGCT TTTTGTTCTG AGAATTTGAT
     1981 TAGTAGTATG AACAAAAGTA AGGAATTAGG GTTCTTGGGT TTTAGGAATT CGATGAAATC
     2041 GTTTGTTTCT TGTATTGATA AGATGAGGGA TTATAGGTTT ATTCCTTAGG CTTCTCTAGC
     2101 TAGAGTCGAT CGACAAGCTC GAGTTTCTCC ATAATAATGT GTGAGTAGTT CCCAGATAAG
     2161 GGAATTAGGG TTCCTATAGG GTTTCGCTCA TGTGTTGAGC ATATAAGAAA CCCTTAGTAT
     2221 GTATTTGTAT TTGTAAAATA CTTCTATCAA TAAAATTTCT AATTCCTAAA ACCAAAATCC
     2281 AGTACTAAAA TCCAGATCGC TGAGCTTGTC TTCTGCACGA AGTGGTTTAA ACTATCAGTG
     2341 TTTGACAGGA TATATTGGCG GGTAAACCTA AGAGAAAAGA GCGTTTATTA GAATAATCGG
     2401 ATATTTAAAA GGGCGTGAAA AGGTTTATCC GTTCGTCCAT TTGTATGTGC ATGCCAACCA
     2461 CAGGGTTCCC CAGATCAGGC GCTGGCTGCT GAACCCCCAG CCGGAACTGA CCCCACAAGG
     2521 CCCTAGCGTT TGCAATGCAC CAGGTCATCA TTGACCCAGG CGTGTTCCAC CAGGCCGCTG
     2581 CCTCGCAACT CTTCGCAGGC TTCGCCGACC TGCTCGCGCC ACTTCTTCAC GCGGGTGGAA
     2641 TCCGATCCGC ACATGAGGCG GAAGGTTTCC AGCTTGAGCG GGTACGGCTC CCGGTGCGAG
     2701 CTGAAATAGT CGAACATCCG TCGGGCCGTC GGCGACAGCT TGCGGTACTT CTCCCATATG
     2761 AATTTCGTGT AGTGGTCGCC AGCAAACAGC ACGACGATTT CCTCGTCGAT CAGGACCTGG
     2821 CAACGGGACG TTTTCTTGCC ACGGTCCAGG ACGCGGAAGC GGTGCAGCAG CGACACCGAT
     2881 TCCAGGTGCC CAACGCGGTC GGACGTGAAG CCCATCGCCG TCGCCTGTAG GCGCGACAGG
     2941 CATTCCTCGG CCTTCGTGTA ATACCGGCCA TTGATCGACC AGCCCAGGTC CTGGCAAAGC
     3001 TCGTAGAACG TGAAGGTGAT CGGCTCGCCG ATAGGGGTGC GCTTCGCGTA CTCCAACACC
     3061 TGCTGCCACA CCAGTTCGTC ATCGTCGGCC CGCAGCTCGA CGCCGGTGTA GGTGATCTTC
     3121 ACGTCCTTGT TGACGTGGAA AATGACCTTG TTTTGCAGCG CCTCGCGCGG GATTTTCTTG
     3181 TTGCGCGTGG TGAACAGGGC AGAGCGGGCC GTGTCGTTTG GCATCGCTCG CATCGTGTCC
     3241 GGCCACGGCG CAATATCGAA CAAGGAAAGC TGCATTTCCT TGATCTGCTG CTTCGTGTGT
     3301 TTCAGCAACG CGGCCTGCTT GGCCTCGCTG ACCTGTTTTG CCAGGTCCTC GCCGGCGGTT
     3361 TTTCGCTTCT TGGTCGTCAT AGTTCCTCGC GTGTCGATGG TCATCGACTT CGCCAAACCT
     3421 GCCGCCTCCT GTTCAAGACG ACGCGAACGC TCCACGGCGG CCGATGGCGC GGGCAGGGCA
     3481 GGGGGAGCCA GTTGCACGCT GTCGCGCTCG ATCTTGGCCG TAGCTTGCTG GACCATCGAG
     3541 CCGACGGACT GGAAGGTTTC GCGGGGCGCA CGCATGACGG TGCGGCTTGC GATGGTTTCG
     3601 GCATCCTCGG CGGAAAACCC CGCGTCGATC AGTTCTTGCC TGTATGCCTT CCGGTCAAAC
     3661 GTCCGATTCA TTCACCCTCC TTGCGGGATT GCCCCGACTC ACGCCGGGGC AATGTGCCCT
     3721 TATTCCTGAT TTGACCCGCC TGGTGCCTTG GTGTCCAGAT AATCCACCTT ATCGGCAATG
     3781 AAGTCGGTCC CGTAGACCGT CTGGCCGTCC TTCTCGTACT TGGTATTCCG AATCTTGCCC
     3841 TGCACGAATA CCAGCGACCC CTTGCCCAAA TACTTGCCGT GGGCCTCGGC CTGAGAGCCA
     3901 AAACACTTGA TGCGGAAGAA GTCGGTGCGC TCCTGCTTGT CGCCGGCATC GTTGCGCCAC
     3961 ATCTAGGATC TGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC
     4021 GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG
     4081 GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA
     4141 CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC
     4201 ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG
     4261 TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT
     4321 CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA
     4381 GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA
     4441 CTAGAAGGAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG
     4501 TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA
     4561 AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG
     4621 GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA
     4681 AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA
     4741 TATATGAGTA AACTTGGTCT GACAGTTACC AATGCTTAAT CAGTGAGGCA CCTATCTCAG
     4801 CGATCTGTCT ATTTCGTTCA TCCATAGTTG CCTGACTCCC CGTCGTGTAG ATAACTACGA
     4861 TACGGGAGGG CTTACCATCT GGCCCCAGTG CTGCAATGAT ACCGCGAGAA CCACGCTCAC
     4921 CGGCTCCAGA TTTATCAGCA ATAAACCAGC CAGCCGGAAG GGCCGAGCGC AGAAGTGGTC
     4981 CTGCAACTTT ATCCGCCTCC ATCCAGTCTA TTAATTGTTG CCGGGAAGCT AGAGTAAGTA
     5041 GTTCGCCAGT TAATAGTTTG CGCAACGTTG TTGCCATTGC TACAGGCATC GTGGTGTCAC
     5101 GCTCGTCGTT TGGTATGGCT TCATTCAGCT CCGGTTCCCA ACGATCAAGG CGAGTTACAT
     5161 GATCCCCCAT GTTGTGCAAA AAAGCGGTTA GCTCCTTCGG TCCTCCGATC GTTGTCAGAA
     5221 GTAAGTTGGC CGCAGTGTTA TCACTCATGG TTATGGCAGC ACTGCATAAT TCTCTTACTG
     5281 TCATGCCATC CGTAAGATGC TTTTCTGTGA CTGGTGAGTA CTCAACCAAG TCATTCTGAG
     5341 AATAGTGTAT GCGGCGACCG AGTTGCTCTT GCCCGGCGTC AATACGGGAT AATACCGCGC
     5401 CACATAGCAG AACTTTAAAA GTGCTCATCA TTGGAAAACG TTCTTCGGGG CGAAAACTCT
     5461 CAAGGATCTT ACCGCTGTTG AGATCCAGTT CGATGTAACC CACTCGTGCA CCCAACTGAT
     5521 CTTCAGCATC TTTTACTTTC ACCAGCGTTT CTGGGTGAGC AAAAACAGGA AGGCAAAATG
     5581 CCGCAAAAAA GGGAATAAGG GCGACACGGA AATGTTGAAT ACTCATACTC TTCCTTTTTC
     5641 AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA
     5701 TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACGAATTG GCCAGCGCTG CCATTTTTGG
     5761 GGTGAGGCCG TTCGCGGCCG AGGGGCGCAG CCCCTGGGGG GATGGGAGGC CCGCGTTAGC
     5821 GGGCCGGGAG GGTTCGAGAA GGGGGGGCAC CCCCCTTCGG CGTGCGCGGT CACGCGCACA
     5881 GGGCGCAGCC CTGGTTAAAA ACAAGGTTTA TAAATATTGG TTTAAAAGCA GGTTAAAAGA
     5941 CAGGTTAGCG GTGGCCGAAA AACGGGCGGA AACCCTTGCA AATGCTGGAT TTTCTGCCTG
     6001 TGGACAGCCC CTCAAATGTC AATAGGTGCG CCCCTCATCT GTCAGCACTC TGCCCCTCAA
     6061 GTGTCAAGGA TCGCGCCCCT CATCTGTCAG TAGTCGCGCC CCTCAAGTGT CAATACCGCA
     6121 GGGCACTTAT CCCCAGGCTT GTCCACATCA TCTGTGGGAA ACTCGCGTAA AATCAGGCGT
     6181 TTTCGCCGAT TTGCGAGGCT GGCCAGCTCC ACGTCGCCGG CCGAAATCGA GCCTGCCCCT
     6241 CATCTGTCAA CGCCGCGCCG GGTGAGTCGG CCCCTCAAGT GTCAACGTCC GCCCCTCATC
     6301 TGTCAGTGAG GGCCAAGTTT TCCGCGAGGT ATCCACAACG CCGGCGGCCG CGGTGTCTCG
     6361 CACACGGCTT CGACGGCGTT TCTGGCGCGT TTGCAGGGCC ATAGACGGCC GCCAGCCCAG
     6421 CGGCGAGGGC AACCAGCCCG G
//