LOCUS       pEPQD1CB0121_(P2_35Ssho 6645 bp ds-DNA     circular     13-APR-2019
DEFINITION  .
FEATURES             Location/Qualifiers
     Misc._feature   30..177
                     /label="LB"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    complement(100..124)
                     /label="LB T-DNA repeat"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     Promoter        233..658
                     /label="CaMV 35S promoter short"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_feature    236..561
                     /label="CaMV 35S promoter repeat unit"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    663..743
                     /label="TMV omega (two 3' bp removed)"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    749..2293
                     /label="CrIO (contains T359S mutation, domesticated, 1 BpiI site and 1 BsmBI site removed) >ENA AHK60833 AHK60833.1 Catharanthus roseus (Madagascar periwinkle) iridoid oxidase "
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     Terminator      2298..2501
                     /label="35S ter"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     Misc._feature   complement(2533..2660)
                     /label="RB short"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    complement(2547..2571)
                     /label="RB T-DNA repeat"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     Origin          complement(2684..4165)
                     /label="RK2"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     CDS             complement(2727..3875)
                     /label="trfA"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     primer_bind     complement(4291..4310)
                     /label="pBR322ori-F"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     CDS             complement(4970..5830)
                     /label="AmpR"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     origin          complement(6028..6645)
                     /label="OriV"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
ORIGIN
        1 TGAGCGTCGC AAAGGAGATC CTGATCTGAC TGATGGGCTG CCTGTATCGA GTGGTGATTT
       61 TGTGCCGAGC TGCCGGTCGG GGAGCTGTTG GCTGGCTGGT GGCAGGATAT ATTGTGGTGT
      121 AAACAAATTG ACGCTTAGAC AACTTAATAA CACATTGCGG ACGTTTTTAA TGTACTGGGG
      181 TGGATGCAGT GGGCCCCACT CTGTGAAGAC AAGCAAGAAT TCAAGCTTGG AGGTCAACAT
      241 GGTGGAGCAC GACACTCTGG TCTACTCCAA AAATGTCAAA GATACAGTCT CAGAAGATCA
      301 AAGGGCTATT GAGACTTTTC AACAAAGGAT AATTTCGGGA AACCTCCTCG GATTCCATTG
      361 CCCAGCTATC TGTCACTTCA TCGAAAGGAC AGTAGAAAAG GAAGGTGGCT CCTACAAATG
      421 CCATCATTGC GATAAAGGAA AGGCTATCAT TCAAGATCTC TCTGCCGACA GTGGTCCCAA
      481 AGATGGACCC CCACCCACGA GGAGCATCGT GGAAAAAGAA GAGGTTCCAA CCACGTCTAC
      541 AAAGCAAGTG GATTGATGTG ACATCTCCAC TGACGTAAGG GATGACGCAC AATCCCACTA
      601 TCCTTCGCAA GACCCTTCCT CTATATAAGG AAGTTCATTT CATTTGGAGA GGACACGCTC
      661 GAGTATAAGA GCTCATTTTT ACAACAATTA CCAACAACAA CAAACAACAA ACAACATTAC
      721 AATTACATTT ACAATTATCG ATACAATGGC GACCATCACT TTCGATTCAC TAAACCCGGT
      781 GACCGTCGCC ATCTCAGCCG GTTTTCTTCT CCTCCTTATC ATCTTTGTGA AATCAAGAAC
      841 TGGTTCATCC AAAAGAAAAC CGCCGGGTCC TCCAGGATGG CCGATTTTCG GCAATATGTT
      901 CGACCTCGGA GATTTACCTC ACCAAACGCT GTACAAGCTC AAGTCCAAAT ACGGGCCGAT
      961 CGTATGGCTC CAGCTCGGCT CAATTAATAC TATGGTTGTT CAAAATGCAG TTTCCGCCGC
     1021 CGAGCTTTTT AAGAAACACG ATGTCCCCTT TTGTGACCGA AAAGTCCCTG ATACTCTCAC
     1081 TGCCTTCAAT TTCAACCAGG GCTCCCTGGG TATGAATACT TACGGCGGAC ATTGGCGAGT
     1141 GCTTAGACGC CTCTGTTCCA TGGAGTTTTT GGTAAATAAA CGTATGAACG AAACTACAGA
     1201 TCTAAGAAGG AGAATTGAGG ACAATATGGT CCGTTGGATT GAAGAAGATT CATTGGCATC
     1261 TAAAGCACAA GGAGGAACCG GAGCGGTTCA ATTGAGCAGG TTTCTTTTTC TAATGGCGTT
     1321 TAATTTGGTG GGTAACCTTA TGTTATCGAG AGATTTAATG GATAATAAGG ATCCAGAAGG
     1381 AAGGGAATTT TTTGATTGTA TGAATGAGAT ATTGGAATTG GCTGGTACTC CTAATATTGC
     1441 TGATTTTTTG CCTTTGTTGA AGAAATTGGA TCCACTTGGA ATGAAGAAAA GGATGGTTGA
     1501 TAATATGTCT AGAACTATGA AAATTTCTTC AAAGTTTGTT CAAGAAAGAC TTGATAATAG
     1561 AAAAGCTGGA AAGATTAATG AAAAGAAAGA TTTCTTGGAT GTTATGCTTG AATACCAAGG
     1621 TGATGGTAAA GATGGTCCCG ATAAATTCAC TGAGCAACAT GTCAACATTG TCATCATGGA
     1681 AATGTTTTTT GCAGGATCAG AGACAACAAG TATCAGCATT GAATGGGGAT TCACAGAACT
     1741 TCTCCGAAAC CCACACGCAT TCAAGAAAGT AAGGGAAGAG ATCGACAGAG TAGTGGGAGT
     1801 AAACAGAATG GTCGAAGAGA GCGACATGGA GAATTTGCCG TATTTGCAAG CAGTTGTAAA
     1861 GGAAACGCTT AGATTGCATC CGGCACTTCC AATGCTTCTA CCAAGAAACA CAATGGAGGA
     1921 CACTGAATAC ATGGGATACC TCATTCCTAA AGGCACACAA GTTTTCGTGA ATGCATGGGC
     1981 AATCGGAAGG GATCCTGAGT ACTGGCAGGA CCCTTTGAGT TTCAAACCAG AAAGGTTTAT
     2041 CAATTCCAGT GTTGAATATA AAGGACAACA TTTTGAATTG ATTCCTTTTG GTTCTGGAAG
     2101 AAGAATTTGT GTGGGATTTC CTTTGGCTCA TAGAGTTGTT CATCTCACTT TGGCTACATT
     2161 AGTCCAGGCT TTTGATTGGG ATCTTGGTGC TGGCGTTAAA CCACAAGATA TTGATTTGGA
     2221 AGAGAGATTG GGATTGACTC TGAGGAAGAA GAATCCATTG AATGTCATCC CTAAGAAGAG
     2281 AGTTCATATC TAAGCTTCTC TAGCTAGAGT CGATCGACAA GCTCGAGTTT CTCCATAATA
     2341 ATGTGTGAGT AGTTCCCAGA TAAGGGAATT AGGGTTCCTA TAGGGTTTCG CTCATGTGTT
     2401 GAGCATATAA GAAACCCTTA GTATGTATTT GTATTTGTAA AATACTTCTA TCAATAAAAT
     2461 TTCTAATTCC TAAAACCAAA ATCCAGTACT AAAATCCAGA TCGCTACTAT TGTCTTCTGC
     2521 ACGAAGTGGT TTAAACTATC AGTGTTTGAC AGGATATATT GGCGGGTAAA CCTAAGAGAA
     2581 AAGAGCGTTT ATTAGAATAA TCGGATATTT AAAAGGGCGT GAAAAGGTTT ATCCGTTCGT
     2641 CCATTTGTAT GTGCATGCCA ACCACAGGGT TCCCCAGATC AGGCGCTGGC TGCTGAACCC
     2701 CCAGCCGGAA CTGACCCCAC AAGGCCCTAG CGTTTGCAAT GCACCAGGTC ATCATTGACC
     2761 CAGGCGTGTT CCACCAGGCC GCTGCCTCGC AACTCTTCGC AGGCTTCGCC GACCTGCTCG
     2821 CGCCACTTCT TCACGCGGGT GGAATCCGAT CCGCACATGA GGCGGAAGGT TTCCAGCTTG
     2881 AGCGGGTACG GCTCCCGGTG CGAGCTGAAA TAGTCGAACA TCCGTCGGGC CGTCGGCGAC
     2941 AGCTTGCGGT ACTTCTCCCA TATGAATTTC GTGTAGTGGT CGCCAGCAAA CAGCACGACG
     3001 ATTTCCTCGT CGATCAGGAC CTGGCAACGG GACGTTTTCT TGCCACGGTC CAGGACGCGG
     3061 AAGCGGTGCA GCAGCGACAC CGATTCCAGG TGCCCAACGC GGTCGGACGT GAAGCCCATC
     3121 GCCGTCGCCT GTAGGCGCGA CAGGCATTCC TCGGCCTTCG TGTAATACCG GCCATTGATC
     3181 GACCAGCCCA GGTCCTGGCA AAGCTCGTAG AACGTGAAGG TGATCGGCTC GCCGATAGGG
     3241 GTGCGCTTCG CGTACTCCAA CACCTGCTGC CACACCAGTT CGTCATCGTC GGCCCGCAGC
     3301 TCGACGCCGG TGTAGGTGAT CTTCACGTCC TTGTTGACGT GGAAAATGAC CTTGTTTTGC
     3361 AGCGCCTCGC GCGGGATTTT CTTGTTGCGC GTGGTGAACA GGGCAGAGCG GGCCGTGTCG
     3421 TTTGGCATCG CTCGCATCGT GTCCGGCCAC GGCGCAATAT CGAACAAGGA AAGCTGCATT
     3481 TCCTTGATCT GCTGCTTCGT GTGTTTCAGC AACGCGGCCT GCTTGGCCTC GCTGACCTGT
     3541 TTTGCCAGGT CCTCGCCGGC GGTTTTTCGC TTCTTGGTCG TCATAGTTCC TCGCGTGTCG
     3601 ATGGTCATCG ACTTCGCCAA ACCTGCCGCC TCCTGTTCAA GACGACGCGA ACGCTCCACG
     3661 GCGGCCGATG GCGCGGGCAG GGCAGGGGGA GCCAGTTGCA CGCTGTCGCG CTCGATCTTG
     3721 GCCGTAGCTT GCTGGACCAT CGAGCCGACG GACTGGAAGG TTTCGCGGGG CGCACGCATG
     3781 ACGGTGCGGC TTGCGATGGT TTCGGCATCC TCGGCGGAAA ACCCCGCGTC GATCAGTTCT
     3841 TGCCTGTATG CCTTCCGGTC AAACGTCCGA TTCATTCACC CTCCTTGCGG GATTGCCCCG
     3901 ACTCACGCCG GGGCAATGTG CCCTTATTCC TGATTTGACC CGCCTGGTGC CTTGGTGTCC
     3961 AGATAATCCA CCTTATCGGC AATGAAGTCG GTCCCGTAGA CCGTCTGGCC GTCCTTCTCG
     4021 TACTTGGTAT TCCGAATCTT GCCCTGCACG AATACCAGCG ACCCCTTGCC CAAATACTTG
     4081 CCGTGGGCCT CGGCCTGAGA GCCAAAACAC TTGATGCGGA AGAAGTCGGT GCGCTCCTGC
     4141 TTGTCGCCGG CATCGTTGCG CCACATCTAG GATCTGCCAG GAACCGTAAA AAGGCCGCGT
     4201 TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA
     4261 GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT
     4321 CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC
     4381 CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG
     4441 TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT
     4501 TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG
     4561 CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA
     4621 AGTGGTGGCC TAACTACGGC TACACTAGAA GGACAGTATT TGGTATCTGC GCTCTGCTGA
     4681 AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG
     4741 GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG
     4801 AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG
     4861 GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT
     4921 GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAAACTTG GTCTGACAGT TACCAATGCT
     4981 TAATCAGTGA GGCACCTATC TCAGCGATCT GTCTATTTCG TTCATCCATA GTTGCCTGAC
     5041 TCCCCGTCGT GTAGATAACT ACGATACGGG AGGGCTTACC ATCTGGCCCC AGTGCTGCAA
     5101 TGATACCGCG AGAACCACGC TCACCGGCTC CAGATTTATC AGCAATAAAC CAGCCAGCCG
     5161 GAAGGGCCGA GCGCAGAAGT GGTCCTGCAA CTTTATCCGC CTCCATCCAG TCTATTAATT
     5221 GTTGCCGGGA AGCTAGAGTA AGTAGTTCGC CAGTTAATAG TTTGCGCAAC GTTGTTGCCA
     5281 TTGCTACAGG CATCGTGGTG TCACGCTCGT CGTTTGGTAT GGCTTCATTC AGCTCCGGTT
     5341 CCCAACGATC AAGGCGAGTT ACATGATCCC CCATGTTGTG CAAAAAAGCG GTTAGCTCCT
     5401 TCGGTCCTCC GATCGTTGTC AGAAGTAAGT TGGCCGCAGT GTTATCACTC ATGGTTATGG
     5461 CAGCACTGCA TAATTCTCTT ACTGTCATGC CATCCGTAAG ATGCTTTTCT GTGACTGGTG
     5521 AGTACTCAAC CAAGTCATTC TGAGAATAGT GTATGCGGCG ACCGAGTTGC TCTTGCCCGG
     5581 CGTCAATACG GGATAATACC GCGCCACATA GCAGAACTTT AAAAGTGCTC ATCATTGGAA
     5641 AACGTTCTTC GGGGCGAAAA CTCTCAAGGA TCTTACCGCT GTTGAGATCC AGTTCGATGT
     5701 AACCCACTCG TGCACCCAAC TGATCTTCAG CATCTTTTAC TTTCACCAGC GTTTCTGGGT
     5761 GAGCAAAAAC AGGAAGGCAA AATGCCGCAA AAAAGGGAAT AAGGGCGACA CGGAAATGTT
     5821 GAATACTCAT ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA
     5881 TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACGA
     5941 ATTGGCCAGC GCTGCCATTT TTGGGGTGAG GCCGTTCGCG GCCGAGGGGC GCAGCCCCTG
     6001 GGGGGATGGG AGGCCCGCGT TAGCGGGCCG GGAGGGTTCG AGAAGGGGGG GCACCCCCCT
     6061 TCGGCGTGCG CGGTCACGCG CACAGGGCGC AGCCCTGGTT AAAAACAAGG TTTATAAATA
     6121 TTGGTTTAAA AGCAGGTTAA AAGACAGGTT AGCGGTGGCC GAAAAACGGG CGGAAACCCT
     6181 TGCAAATGCT GGATTTTCTG CCTGTGGACA GCCCCTCAAA TGTCAATAGG TGCGCCCCTC
     6241 ATCTGTCAGC ACTCTGCCCC TCAAGTGTCA AGGATCGCGC CCCTCATCTG TCAGTAGTCG
     6301 CGCCCCTCAA GTGTCAATAC CGCAGGGCAC TTATCCCCAG GCTTGTCCAC ATCATCTGTG
     6361 GGAAACTCGC GTAAAATCAG GCGTTTTCGC CGATTTGCGA GGCTGGCCAG CTCCACGTCG
     6421 CCGGCCGAAA TCGAGCCTGC CCCTCATCTG TCAACGCCGC GCCGGGTGAG TCGGCCCCTC
     6481 AAGTGTCAAC GTCCGCCCCT CATCTGTCAG TGAGGGCCAA GTTTTCCGCG AGGTATCCAC
     6541 AACGCCGGCG GCCGCGGTGT CTCGCACACG GCTTCGACGG CGTTTCTGGC GCGTTTGCAG
     6601 GGCCATAGAC GGCCGCCAGC CCAGCGGCGA GGGCAACCAG CCCGG
//