LOCUS       pEPQD1CB0767_(P4_35Ssho 6663 bp ds-DNA     circular     12-OCT-2020
DEFINITION  .
FEATURES             Location/Qualifiers
     Misc._feature   22..169
                     /label="LB"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     primer          34..56
                     /label="QMD671_GB_LB_seq_F"
                     /note="sequence: ctgtatcgagtggtgattttgtg"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer          88..111
                     /label="214or (L1, REV, LB)"
                     /note="sequence: CTGGTGGCAGGATATATTGTGGTG"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    complement(92..116)
                     /label="LB T-DNA repeat"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     primer          106..126
                     /label="226or (L2, FWD, LB)"
                     /note="sequence: GTGGTGTAAACAAATTGACGC"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     primer          complement(106..127)
                     /label="QMD541_LB_rev_seq(226or_binding)"
                     /note="sequence: AGCGTCAATTTGTTTACACCAC"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     Promoter        225..650
                     /label="CaMV 35S promoter short"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     None            228..553
                     /label="CaMV 35S promoter repeat unit"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     primer          complement(554..573)
                     /label="QMD079_CaMV35Sprom_seq_R"
                     /note="sequence: CCCTTACGTCAGTGGAGATG"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     primer          574..594
                     /label="QMD325_35SseqR_atQMD311site_R"
                     /note="sequence: ATGACGCACAATCCCACTATC"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     primer          complement(576..596)
                     /label="QMD311_CaMV35Sprom_seq_R"
                     /note="sequence: AGGATAGTGGGATTGTGCGTC"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     None            655..735
                     /label="TMV omega (two 3' bp removed)"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    741..2300
                     /label="Cr7DLH 7-deoxyloganic acid hydroxylase CDS, Catharanthus roseus, KF302067  Caros005234 (domesticated, 1 BtgZI and 1 BsmBI site removed)"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     Terminator      2308..2511
                     /label="35S ter"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    complement(2539..2664)
                     /label="RB"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    complement(2557..2581)
                     /label="RB T-DNA repeat"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer          complement(2575..2599)
                     /label="QMD315_LB_Level2/M_seq_R"
                     /note="sequence: aacgctcttttctcttaggtttacc"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     primer          complement(2625..2644)
                     /label="227or (L2, REV, RB)"
                     /note="sequence: GGATAAACCTTTTCACGCCC"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     primer          complement(2657..2682)
                     /label="213or (L1, FWD, RB)"
                     /note="sequence: GAACCCTGTGGTTGGCATGCACATAC"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     primer          complement(2737..2755)
                     /label="QMD314_TrfA_LMoutside_F"
                     /note="sequence: ggtgcattgcaaacgctag"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     CDS             complement(2737..3885)
                     /label="Plasmid replication initiator protein TrfA, P07676 (TRFA_ECOLX)"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     rep_origin      complement(4221..4809)
                     /label="pBR322 origin of replication (derivative of pMB1/ColE1)"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     primer_bind     complement(4266..4284)
                     /label="QMD062 primer"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     primer          complement(4266..4284)
                     /label="QMD062_pJL1_outside(BR322)_F"
                     /note="sequence: tcgccacctctgacttgag"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer          complement(4268..4287)
                     /label="QMD132_LETprom2_F, 61.4"
                     /note="sequence: gtttcgccacctctgacttg"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer          complement(4297..4320)
                     /label="QMD131_LETprom1_F, 61.7, -3.89 self"
                     /note="sequence: gggaaacgcctggtatctttatag"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     primer_bind     complement(4301..4320)
                     /label="pBR322ori-F"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     primer          4766..4787
                     /label="QMD540_pBR322_seq_R"
                     /note="sequence: ttgtttgcaagcagcagattac"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     CDS             complement(4980..5840)
                     /label="beta-lactamase, ampicillin resistance (ampR)"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer          complement(5107..5123)
                     /label="QMD047 binding region (57.3)"
                     /note="sequence: TCTCGCGGTATCATTGC"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     primer          5885..5912
                     /label="212or (L0 REV)"
                     /note="sequence: GTCTCATGAGCGGATACATATTTGAATG"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     rep_origin      complement(6032..6663)
                     /label="RK2 (OriV) origin of replication (incP incompatibility group)"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
ORIGIN
        1 GCAAAGGAGA TCCTGATCTG ACTGATGGGC TGCCTGTATC GAGTGGTGAT TTTGTGCCGA
       61 GCTGCCGGTC GGGGAGCTGT TGGCTGGCTG GTGGCAGGAT ATATTGTGGT GTAAACAAAT
      121 TGACGCTTAG ACAACTTAAT AACACATTGC GGACGTTTTT AATGTACTGG GGTGGATGCA
      181 GTGGGCCCCA CTCTGTGAAG ACAATTACGA ATTCCCATGG GGAGGTCAAC ATGGTGGAGC
      241 ACGACACTCT GGTCTACTCC AAAAATGTCA AAGATACAGT CTCAGAAGAT CAAAGGGCTA
      301 TTGAGACTTT TCAACAAAGG ATAATTTCGG GAAACCTCCT CGGATTCCAT TGCCCAGCTA
      361 TCTGTCACTT CATCGAAAGG ACAGTAGAAA AGGAAGGTGG CTCCTACAAA TGCCATCATT
      421 GCGATAAAGG AAAGGCTATC ATTCAAGATC TCTCTGCCGA CAGTGGTCCC AAAGATGGAC
      481 CCCCACCCAC GAGGAGCATC GTGGAAAAAG AAGAGGTTCC AACCACGTCT ACAAAGCAAG
      541 TGGATTGATG TGACATCTCC ACTGACGTAA GGGATGACGC ACAATCCCAC TATCCTTCGC
      601 AAGACCCTTC CTCTATATAA GGAAGTTCAT TTCATTTGGA GAGGACACGC TCGAGTATAA
      661 GAGCTCATTT TTACAACAAT TACCAACAAC AACAAACAAC AAACAACATT ACAATTACAT
      721 TTACAATTAT CGATACAATG GAATTGAACT TCAAATCAAT TATTTTCTTG GTTTTTGTTA
      781 GTCTAACCCT TTACTGGGTC TATAGAATCT TGGATTGGGT ATGGTTTAAG CCCAAGAAGC
      841 TGGAAAAATG CTTAAGAGAA CAAGGGTTTA AGGGAAATCC TTACAGATTG TTCTTGGGAG
      901 ATCAGTATGA TAGTGGAAAA TTGATCAGAC AAGCCTTGAC TAAACCTATT GGTGTTGAAG
      961 AAGATGTTAA GAAACGAATT GTACCTCATA TACTCAAAAC TGTTGGGACT CATGGCAAGA
     1021 AGTCTTTTAT GTGGGTTGGA AGAATACCAA GGGTGAATAT CACAGATCCA GAGCTGATTA
     1081 AAGAGGTCTT AACAAAATAC TATAAGTTCC AGAAGAATCA TCATGATCTT GACCCAATTA
     1141 CCAAATTGCT TTTGACTGGA ATTGGAAGCT TAGAAGGTGA CCCTTGGGCT AAACGCCGAA
     1201 AAATCATCAA TGCTGCTTTT CACTTTGAAA AGTTAAAGCT TATGCTTCCT GCATTCTATT
     1261 TGAGTTGCCG TGATATGGTG ACAAAATGGG ATAATAAGGT CCCTGAAGGA GGATCAGCAG
     1321 AGGTGGATGT GTGGCATGAT ATTGAAACCT TAACTGGAGA TGTAATTTCA AGAACATTAT
     1381 TTGGAAGTAA CTTCGAGGAA GGGAGAAGAA TATTTGAACT CATGAAAGAA CTTACTGCTC
     1441 TTACTATTGA TGTCATTCGC TCGGTTTATA TCCCTGGACA GAGGTTTCTT CCAACTAAGA
     1501 GGAACAATAG GATGAGAGCA ATTGACAAAG AAGTTAGAGT AAGAATAACA GAAATTATCA
     1561 ATAAGAAAAT GAAGGTAATG AAATCAGGAG AAGCAGCAAG TGCTGCTGAT GATTTTTTGG
     1621 GAATATTGTT GGAATGCAAT CTGAATGAAA TAAAGGAGCA AGGAAATAAC AAAAGTGCTG
     1681 GAATGACTAT TGGAGAGATT ATTGGTGAGT GCAAACTCTT CTATTTTGCT GGCCAGGACA
     1741 CTACCTCGAC TTTACTCGTG TGGACAATGG TCCTCTTGTC TAGATTCCCC GAATGGCAAA
     1801 CTCGTGCCAG AGAAGAAGTT TTTCAAGTCT TTGGTAATAA AACCCCGGAC TATGATGGTA
     1861 TCAGCCATCT TAAAGTTATA ACGATGATCT TGTACGAGGT TTTGAGGTTA TATACTCCAG
     1921 TGGCTGAACT GACAAAAGTG GCTCATGAAG CTACACAACT GGGAAAATAC TTCATTCCAG
     1981 CGGGCGTGCA GCTAATGATG CCACAAATCC TACTTCATCA TGACCCTGAA ATATGGGGTG
     2041 AAGATGTAAT GGAGTTCAAA CCAGAAAGAT TTGCAGAGGG AGTTCTCAAG GCAACTAAGA
     2101 GCCAAGGCTC TTTTTTTCCA TTTAGCTTGG GACCAAGAAT GTGCATTGGC CAAAACTTTG
     2161 CTCTATTGGA AGCCAAAATG GCAATGTCTC TAATCTTGCG TAGATTTTCT TTCGAGCTTT
     2221 CTCCCTCCTA TGTTCATGCT CCTTTCACTC TCATCACAAT GCAACCCCAA TATGGTGCTC
     2281 ATTTGATTCT GCACAAACTC TAAGCTTCTC TAGCTAGAGT CGATCGACAA GCTCGAGTTT
     2341 CTCCATAATA ATGTGTGAGT AGTTCCCAGA TAAGGGAATT AGGGTTCCTA TAGGGTTTCG
     2401 CTCATGTGTT GAGCATATAA GAAACCCTTA GTATGTATTT GTATTTGTAA AATACTTCTA
     2461 TCAATAAAAT TTCTAATTCC TAAAACCAAA ATCCAGTACT AAAATCCAGA TCGCTCAGAT
     2521 TGTCTTCTGC ACGAAGTGGT TTAAACTATC AGTGTTTGAC AGGATATATT GGCGGGTAAA
     2581 CCTAAGAGAA AAGAGCGTTT ATTAGAATAA TCGGATATTT AAAAGGGCGT GAAAAGGTTT
     2641 ATCCGTTCGT CCATTTGTAT GTGCATGCCA ACCACAGGGT TCCCCAGATC AGGCGCTGGC
     2701 TGCTGAACCC CCAGCCGGAA CTGACCCCAC AAGGCCCTAG CGTTTGCAAT GCACCAGGTC
     2761 ATCATTGACC CAGGCGTGTT CCACCAGGCC GCTGCCTCGC AACTCTTCGC AGGCTTCGCC
     2821 GACCTGCTCG CGCCACTTCT TCACGCGGGT GGAATCCGAT CCGCACATGA GGCGGAAGGT
     2881 TTCCAGCTTG AGCGGGTACG GCTCCCGGTG CGAGCTGAAA TAGTCGAACA TCCGTCGGGC
     2941 CGTCGGCGAC AGCTTGCGGT ACTTCTCCCA TATGAATTTC GTGTAGTGGT CGCCAGCAAA
     3001 CAGCACGACG ATTTCCTCGT CGATCAGGAC CTGGCAACGG GACGTTTTCT TGCCACGGTC
     3061 CAGGACGCGG AAGCGGTGCA GCAGCGACAC CGATTCCAGG TGCCCAACGC GGTCGGACGT
     3121 GAAGCCCATC GCCGTCGCCT GTAGGCGCGA CAGGCATTCC TCGGCCTTCG TGTAATACCG
     3181 GCCATTGATC GACCAGCCCA GGTCCTGGCA AAGCTCGTAG AACGTGAAGG TGATCGGCTC
     3241 GCCGATAGGG GTGCGCTTCG CGTACTCCAA CACCTGCTGC CACACCAGTT CGTCATCGTC
     3301 GGCCCGCAGC TCGACGCCGG TGTAGGTGAT CTTCACGTCC TTGTTGACGT GGAAAATGAC
     3361 CTTGTTTTGC AGCGCCTCGC GCGGGATTTT CTTGTTGCGC GTGGTGAACA GGGCAGAGCG
     3421 GGCCGTGTCG TTTGGCATCG CTCGCATCGT GTCCGGCCAC GGCGCAATAT CGAACAAGGA
     3481 AAGCTGCATT TCCTTGATCT GCTGCTTCGT GTGTTTCAGC AACGCGGCCT GCTTGGCCTC
     3541 GCTGACCTGT TTTGCCAGGT CCTCGCCGGC GGTTTTTCGC TTCTTGGTCG TCATAGTTCC
     3601 TCGCGTGTCG ATGGTCATCG ACTTCGCCAA ACCTGCCGCC TCCTGTTCAA GACGACGCGA
     3661 ACGCTCCACG GCGGCCGATG GCGCGGGCAG GGCAGGGGGA GCCAGTTGCA CGCTGTCGCG
     3721 CTCGATCTTG GCCGTAGCTT GCTGGACCAT CGAGCCGACG GACTGGAAGG TTTCGCGGGG
     3781 CGCACGCATG ACGGTGCGGC TTGCGATGGT TTCGGCATCC TCGGCGGAAA ACCCCGCGTC
     3841 GATCAGTTCT TGCCTGTATG CCTTCCGGTC AAACGTCCGA TTCATTCACC CTCCTTGCGG
     3901 GATTGCCCCG ACTCACGCCG GGGCAATGTG CCCTTATTCC TGATTTGACC CGCCTGGTGC
     3961 CTTGGTGTCC AGATAATCCA CCTTATCGGC AATGAAGTCG GTCCCGTAGA CCGTCTGGCC
     4021 GTCCTTCTCG TACTTGGTAT TCCGAATCTT GCCCTGCACG AATACCAGCG ACCCCTTGCC
     4081 CAAATACTTG CCGTGGGCCT CGGCCTGAGA GCCAAAACAC TTGATGCGGA AGAAGTCGGT
     4141 GCGCTCCTGC TTGTCGCCGG CATCGTTGCG CCACATCTAG GATCTGCCAG GAACCGTAAA
     4201 AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT
     4261 CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC
     4321 CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC
     4381 GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT
     4441 TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC
     4501 CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG
     4561 CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA
     4621 GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GGACAGTATT TGGTATCTGC
     4681 GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA
     4741 ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA
     4801 GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC
     4861 TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA
     4921 AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAAACTTG GTCTGACAGT
     4981 TACCAATGCT TAATCAGTGA GGCACCTATC TCAGCGATCT GTCTATTTCG TTCATCCATA
     5041 GTTGCCTGAC TCCCCGTCGT GTAGATAACT ACGATACGGG AGGGCTTACC ATCTGGCCCC
     5101 AGTGCTGCAA TGATACCGCG AGAACCACGC TCACCGGCTC CAGATTTATC AGCAATAAAC
     5161 CAGCCAGCCG GAAGGGCCGA GCGCAGAAGT GGTCCTGCAA CTTTATCCGC CTCCATCCAG
     5221 TCTATTAATT GTTGCCGGGA AGCTAGAGTA AGTAGTTCGC CAGTTAATAG TTTGCGCAAC
     5281 GTTGTTGCCA TTGCTACAGG CATCGTGGTG TCACGCTCGT CGTTTGGTAT GGCTTCATTC
     5341 AGCTCCGGTT CCCAACGATC AAGGCGAGTT ACATGATCCC CCATGTTGTG CAAAAAAGCG
     5401 GTTAGCTCCT TCGGTCCTCC GATCGTTGTC AGAAGTAAGT TGGCCGCAGT GTTATCACTC
     5461 ATGGTTATGG CAGCACTGCA TAATTCTCTT ACTGTCATGC CATCCGTAAG ATGCTTTTCT
     5521 GTGACTGGTG AGTACTCAAC CAAGTCATTC TGAGAATAGT GTATGCGGCG ACCGAGTTGC
     5581 TCTTGCCCGG CGTCAATACG GGATAATACC GCGCCACATA GCAGAACTTT AAAAGTGCTC
     5641 ATCATTGGAA AACGTTCTTC GGGGCGAAAA CTCTCAAGGA TCTTACCGCT GTTGAGATCC
     5701 AGTTCGATGT AACCCACTCG TGCACCCAAC TGATCTTCAG CATCTTTTAC TTTCACCAGC
     5761 GTTTCTGGGT GAGCAAAAAC AGGAAGGCAA AATGCCGCAA AAAAGGGAAT AAGGGCGACA
     5821 CGGAAATGTT GAATACTCAT ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT
     5881 TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT
     5941 CCGCGCACGA ATTGGCCAGC GCTGCCATTT TTGGGGTGAG GCCGTTCGCG GCCGAGGGGC
     6001 GCAGCCCCTG GGGGGATGGG AGGCCCGCGT TAGCGGGCCG GGAGGGTTCG AGAAGGGGGG
     6061 GCACCCCCCT TCGGCGTGCG CGGTCACGCG CACAGGGCGC AGCCCTGGTT AAAAACAAGG
     6121 TTTATAAATA TTGGTTTAAA AGCAGGTTAA AAGACAGGTT AGCGGTGGCC GAAAAACGGG
     6181 CGGAAACCCT TGCAAATGCT GGATTTTCTG CCTGTGGACA GCCCCTCAAA TGTCAATAGG
     6241 TGCGCCCCTC ATCTGTCAGC ACTCTGCCCC TCAAGTGTCA AGGATCGCGC CCCTCATCTG
     6301 TCAGTAGTCG CGCCCCTCAA GTGTCAATAC CGCAGGGCAC TTATCCCCAG GCTTGTCCAC
     6361 ATCATCTGTG GGAAACTCGC GTAAAATCAG GCGTTTTCGC CGATTTGCGA GGCTGGCCAG
     6421 CTCCACGTCG CCGGCCGAAA TCGAGCCTGC CCCTCATCTG TCAACGCCGC GCCGGGTGAG
     6481 TCGGCCCCTC AAGTGTCAAC GTCCGCCCCT CATCTGTCAG TGAGGGCCAA GTTTTCCGCG
     6541 AGGTATCCAC AACGCCGGCG GCCGCGGTGT CTCGCACACG GCTTCGACGG CGTTTCTGGC
     6601 GCGTTTGCAG GGCCATAGAC GGCCGCCAGC CCAGCGGCGA GGGCAACCAG CCCGGTGAGC
     6661 GTC
//