LOCUS       pEPQD1CB0768_(P4_35Ssho 6840 bp ds-DNA     circular     12-OCT-2020
DEFINITION  .
FEATURES             Location/Qualifiers
     Misc._feature   22..169
                     /label="LB"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     primer          34..56
                     /label="QMD671_GB_LB_seq_F"
                     /note="sequence: ctgtatcgagtggtgattttgtg"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer          88..111
                     /label="214or (L1, REV, LB)"
                     /note="sequence: CTGGTGGCAGGATATATTGTGGTG"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    complement(92..116)
                     /label="LB T-DNA repeat"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     primer          106..126
                     /label="226or (L2, FWD, LB)"
                     /note="sequence: GTGGTGTAAACAAATTGACGC"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     primer          complement(106..127)
                     /label="QMD541_LB_rev_seq(226or_binding)"
                     /note="sequence: AGCGTCAATTTGTTTACACCAC"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     Promoter        225..650
                     /label="CaMV 35S promoter short"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     None            228..553
                     /label="CaMV 35S promoter repeat unit"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     primer          complement(554..573)
                     /label="QMD079_CaMV35Sprom_seq_R"
                     /note="sequence: CCCTTACGTCAGTGGAGATG"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     primer          574..594
                     /label="QMD325_35SseqR_atQMD311site_R"
                     /note="sequence: ATGACGCACAATCCCACTATC"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     primer          complement(576..596)
                     /label="QMD311_CaMV35Sprom_seq_R"
                     /note="sequence: AGGATAGTGGGATTGTGCGTC"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     Golden_Gate_Par 651..917
                     /label="5U-TMV+SP-chloroplast"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     5'_UTR          655..737
                     /label="TMV omega"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     Transit_peptide 738..913
                     /label="chloroplast targeting signal"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     CDS             738..2480
                     /label="Translation 738-2480"
     misc_feature    918..2477
                     /label="Cr7DLH 7-deoxyloganic acid hydroxylase CDS, Catharanthus roseus, KF302067  Caros005234 (domesticated, 1 BtgZI and 1 BsmBI site removed)"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     Terminator      2485..2688
                     /label="35S ter"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    complement(2716..2841)
                     /label="RB"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    complement(2734..2758)
                     /label="RB T-DNA repeat"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer          complement(2752..2776)
                     /label="QMD315_LB_Level2/M_seq_R"
                     /note="sequence: aacgctcttttctcttaggtttacc"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     primer          complement(2802..2821)
                     /label="227or (L2, REV, RB)"
                     /note="sequence: GGATAAACCTTTTCACGCCC"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     primer          complement(2834..2859)
                     /label="213or (L1, FWD, RB)"
                     /note="sequence: GAACCCTGTGGTTGGCATGCACATAC"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     primer          complement(2914..2932)
                     /label="QMD314_TrfA_LMoutside_F"
                     /note="sequence: ggtgcattgcaaacgctag"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     CDS             complement(2914..4062)
                     /label="Plasmid replication initiator protein TrfA, P07676 (TRFA_ECOLX)"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     rep_origin      complement(4398..4986)
                     /label="pBR322 origin of replication (derivative of pMB1/ColE1)"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     primer_bind     complement(4443..4461)
                     /label="QMD062 primer"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     primer          complement(4443..4461)
                     /label="QMD062_pJL1_outside(BR322)_F"
                     /note="sequence: tcgccacctctgacttgag"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer          complement(4445..4464)
                     /label="QMD132_LETprom2_F, 61.4"
                     /note="sequence: gtttcgccacctctgacttg"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer          complement(4474..4497)
                     /label="QMD131_LETprom1_F, 61.7, -3.89 self"
                     /note="sequence: gggaaacgcctggtatctttatag"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     primer_bind     complement(4478..4497)
                     /label="pBR322ori-F"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     primer          4943..4964
                     /label="QMD540_pBR322_seq_R"
                     /note="sequence: ttgtttgcaagcagcagattac"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     CDS             complement(5157..6017)
                     /label="beta-lactamase, ampicillin resistance (ampR)"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer          complement(5284..5300)
                     /label="QMD047 binding region (57.3)"
                     /note="sequence: TCTCGCGGTATCATTGC"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     primer          6062..6089
                     /label="212or (L0 REV)"
                     /note="sequence: GTCTCATGAGCGGATACATATTTGAATG"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     rep_origin      complement(6209..6840)
                     /label="RK2 (OriV) origin of replication (incP incompatibility group)"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
ORIGIN
        1 GCAAAGGAGA TCCTGATCTG ACTGATGGGC TGCCTGTATC GAGTGGTGAT TTTGTGCCGA
       61 GCTGCCGGTC GGGGAGCTGT TGGCTGGCTG GTGGCAGGAT ATATTGTGGT GTAAACAAAT
      121 TGACGCTTAG ACAACTTAAT AACACATTGC GGACGTTTTT AATGTACTGG GGTGGATGCA
      181 GTGGGCCCCA CTCTGTGAAG ACAATTACGA ATTCCCATGG GGAGGTCAAC ATGGTGGAGC
      241 ACGACACTCT GGTCTACTCC AAAAATGTCA AAGATACAGT CTCAGAAGAT CAAAGGGCTA
      301 TTGAGACTTT TCAACAAAGG ATAATTTCGG GAAACCTCCT CGGATTCCAT TGCCCAGCTA
      361 TCTGTCACTT CATCGAAAGG ACAGTAGAAA AGGAAGGTGG CTCCTACAAA TGCCATCATT
      421 GCGATAAAGG AAAGGCTATC ATTCAAGATC TCTCTGCCGA CAGTGGTCCC AAAGATGGAC
      481 CCCCACCCAC GAGGAGCATC GTGGAAAAAG AAGAGGTTCC AACCACGTCT ACAAAGCAAG
      541 TGGATTGATG TGACATCTCC ACTGACGTAA GGGATGACGC ACAATCCCAC TATCCTTCGC
      601 AAGACCCTTC CTCTATATAA GGAAGTTCAT TTCATTTGGA GAGGACACGC TACTGTATAA
      661 GAGCTCATTT TTACAACAAT TACCAACAAC AACAAACAAC AAACAACATT ACAATTACAT
      721 TTACAATTAT CGATAACATG GCTTCTTCTA TGCTTTCTTC TGCTGCTGTT GTTGCTACTC
      781 GTGCTAGTGC TGCTCAAGCT AGTATGGTTG CTCCTTTTAC TGGACTTAAG TCTGCTGCTT
      841 CTTTTCCTGT TACTAGAAAG CAAAACAACC TTGATATTAC TTCTATTGCT AGTAACGGAG
      901 GAAGAGTTCG AGCAATGGAA TTGAACTTCA AATCAATTAT TTTCTTGGTT TTTGTTAGTC
      961 TAACCCTTTA CTGGGTCTAT AGAATCTTGG ATTGGGTATG GTTTAAGCCC AAGAAGCTGG
     1021 AAAAATGCTT AAGAGAACAA GGGTTTAAGG GAAATCCTTA CAGATTGTTC TTGGGAGATC
     1081 AGTATGATAG TGGAAAATTG ATCAGACAAG CCTTGACTAA ACCTATTGGT GTTGAAGAAG
     1141 ATGTTAAGAA ACGAATTGTA CCTCATATAC TCAAAACTGT TGGGACTCAT GGCAAGAAGT
     1201 CTTTTATGTG GGTTGGAAGA ATACCAAGGG TGAATATCAC AGATCCAGAG CTGATTAAAG
     1261 AGGTCTTAAC AAAATACTAT AAGTTCCAGA AGAATCATCA TGATCTTGAC CCAATTACCA
     1321 AATTGCTTTT GACTGGAATT GGAAGCTTAG AAGGTGACCC TTGGGCTAAA CGCCGAAAAA
     1381 TCATCAATGC TGCTTTTCAC TTTGAAAAGT TAAAGCTTAT GCTTCCTGCA TTCTATTTGA
     1441 GTTGCCGTGA TATGGTGACA AAATGGGATA ATAAGGTCCC TGAAGGAGGA TCAGCAGAGG
     1501 TGGATGTGTG GCATGATATT GAAACCTTAA CTGGAGATGT AATTTCAAGA ACATTATTTG
     1561 GAAGTAACTT CGAGGAAGGG AGAAGAATAT TTGAACTCAT GAAAGAACTT ACTGCTCTTA
     1621 CTATTGATGT CATTCGCTCG GTTTATATCC CTGGACAGAG GTTTCTTCCA ACTAAGAGGA
     1681 ACAATAGGAT GAGAGCAATT GACAAAGAAG TTAGAGTAAG AATAACAGAA ATTATCAATA
     1741 AGAAAATGAA GGTAATGAAA TCAGGAGAAG CAGCAAGTGC TGCTGATGAT TTTTTGGGAA
     1801 TATTGTTGGA ATGCAATCTG AATGAAATAA AGGAGCAAGG AAATAACAAA AGTGCTGGAA
     1861 TGACTATTGG AGAGATTATT GGTGAGTGCA AACTCTTCTA TTTTGCTGGC CAGGACACTA
     1921 CCTCGACTTT ACTCGTGTGG ACAATGGTCC TCTTGTCTAG ATTCCCCGAA TGGCAAACTC
     1981 GTGCCAGAGA AGAAGTTTTT CAAGTCTTTG GTAATAAAAC CCCGGACTAT GATGGTATCA
     2041 GCCATCTTAA AGTTATAACG ATGATCTTGT ACGAGGTTTT GAGGTTATAT ACTCCAGTGG
     2101 CTGAACTGAC AAAAGTGGCT CATGAAGCTA CACAACTGGG AAAATACTTC ATTCCAGCGG
     2161 GCGTGCAGCT AATGATGCCA CAAATCCTAC TTCATCATGA CCCTGAAATA TGGGGTGAAG
     2221 ATGTAATGGA GTTCAAACCA GAAAGATTTG CAGAGGGAGT TCTCAAGGCA ACTAAGAGCC
     2281 AAGGCTCTTT TTTTCCATTT AGCTTGGGAC CAAGAATGTG CATTGGCCAA AACTTTGCTC
     2341 TATTGGAAGC CAAAATGGCA ATGTCTCTAA TCTTGCGTAG ATTTTCTTTC GAGCTTTCTC
     2401 CCTCCTATGT TCATGCTCCT TTCACTCTCA TCACAATGCA ACCCCAATAT GGTGCTCATT
     2461 TGATTCTGCA CAAACTCTAA GCTTCTCTAG CTAGAGTCGA TCGACAAGCT CGAGTTTCTC
     2521 CATAATAATG TGTGAGTAGT TCCCAGATAA GGGAATTAGG GTTCCTATAG GGTTTCGCTC
     2581 ATGTGTTGAG CATATAAGAA ACCCTTAGTA TGTATTTGTA TTTGTAAAAT ACTTCTATCA
     2641 ATAAAATTTC TAATTCCTAA AACCAAAATC CAGTACTAAA ATCCAGATCG CTCAGATTGT
     2701 CTTCTGCACG AAGTGGTTTA AACTATCAGT GTTTGACAGG ATATATTGGC GGGTAAACCT
     2761 AAGAGAAAAG AGCGTTTATT AGAATAATCG GATATTTAAA AGGGCGTGAA AAGGTTTATC
     2821 CGTTCGTCCA TTTGTATGTG CATGCCAACC ACAGGGTTCC CCAGATCAGG CGCTGGCTGC
     2881 TGAACCCCCA GCCGGAACTG ACCCCACAAG GCCCTAGCGT TTGCAATGCA CCAGGTCATC
     2941 ATTGACCCAG GCGTGTTCCA CCAGGCCGCT GCCTCGCAAC TCTTCGCAGG CTTCGCCGAC
     3001 CTGCTCGCGC CACTTCTTCA CGCGGGTGGA ATCCGATCCG CACATGAGGC GGAAGGTTTC
     3061 CAGCTTGAGC GGGTACGGCT CCCGGTGCGA GCTGAAATAG TCGAACATCC GTCGGGCCGT
     3121 CGGCGACAGC TTGCGGTACT TCTCCCATAT GAATTTCGTG TAGTGGTCGC CAGCAAACAG
     3181 CACGACGATT TCCTCGTCGA TCAGGACCTG GCAACGGGAC GTTTTCTTGC CACGGTCCAG
     3241 GACGCGGAAG CGGTGCAGCA GCGACACCGA TTCCAGGTGC CCAACGCGGT CGGACGTGAA
     3301 GCCCATCGCC GTCGCCTGTA GGCGCGACAG GCATTCCTCG GCCTTCGTGT AATACCGGCC
     3361 ATTGATCGAC CAGCCCAGGT CCTGGCAAAG CTCGTAGAAC GTGAAGGTGA TCGGCTCGCC
     3421 GATAGGGGTG CGCTTCGCGT ACTCCAACAC CTGCTGCCAC ACCAGTTCGT CATCGTCGGC
     3481 CCGCAGCTCG ACGCCGGTGT AGGTGATCTT CACGTCCTTG TTGACGTGGA AAATGACCTT
     3541 GTTTTGCAGC GCCTCGCGCG GGATTTTCTT GTTGCGCGTG GTGAACAGGG CAGAGCGGGC
     3601 CGTGTCGTTT GGCATCGCTC GCATCGTGTC CGGCCACGGC GCAATATCGA ACAAGGAAAG
     3661 CTGCATTTCC TTGATCTGCT GCTTCGTGTG TTTCAGCAAC GCGGCCTGCT TGGCCTCGCT
     3721 GACCTGTTTT GCCAGGTCCT CGCCGGCGGT TTTTCGCTTC TTGGTCGTCA TAGTTCCTCG
     3781 CGTGTCGATG GTCATCGACT TCGCCAAACC TGCCGCCTCC TGTTCAAGAC GACGCGAACG
     3841 CTCCACGGCG GCCGATGGCG CGGGCAGGGC AGGGGGAGCC AGTTGCACGC TGTCGCGCTC
     3901 GATCTTGGCC GTAGCTTGCT GGACCATCGA GCCGACGGAC TGGAAGGTTT CGCGGGGCGC
     3961 ACGCATGACG GTGCGGCTTG CGATGGTTTC GGCATCCTCG GCGGAAAACC CCGCGTCGAT
     4021 CAGTTCTTGC CTGTATGCCT TCCGGTCAAA CGTCCGATTC ATTCACCCTC CTTGCGGGAT
     4081 TGCCCCGACT CACGCCGGGG CAATGTGCCC TTATTCCTGA TTTGACCCGC CTGGTGCCTT
     4141 GGTGTCCAGA TAATCCACCT TATCGGCAAT GAAGTCGGTC CCGTAGACCG TCTGGCCGTC
     4201 CTTCTCGTAC TTGGTATTCC GAATCTTGCC CTGCACGAAT ACCAGCGACC CCTTGCCCAA
     4261 ATACTTGCCG TGGGCCTCGG CCTGAGAGCC AAAACACTTG ATGCGGAAGA AGTCGGTGCG
     4321 CTCCTGCTTG TCGCCGGCAT CGTTGCGCCA CATCTAGGAT CTGCCAGGAA CCGTAAAAAG
     4381 GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA
     4441 CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT
     4501 GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC
     4561 TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG
     4621 GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC
     4681 TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA
     4741 CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG
     4801 TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGGA CAGTATTTGG TATCTGCGCT
     4861 CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC
     4921 ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA
     4981 TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA
     5041 CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT
     5101 TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AAACTTGGTC TGACAGTTAC
     5161 CAATGCTTAA TCAGTGAGGC ACCTATCTCA GCGATCTGTC TATTTCGTTC ATCCATAGTT
     5221 GCCTGACTCC CCGTCGTGTA GATAACTACG ATACGGGAGG GCTTACCATC TGGCCCCAGT
     5281 GCTGCAATGA TACCGCGAGA ACCACGCTCA CCGGCTCCAG ATTTATCAGC AATAAACCAG
     5341 CCAGCCGGAA GGGCCGAGCG CAGAAGTGGT CCTGCAACTT TATCCGCCTC CATCCAGTCT
     5401 ATTAATTGTT GCCGGGAAGC TAGAGTAAGT AGTTCGCCAG TTAATAGTTT GCGCAACGTT
     5461 GTTGCCATTG CTACAGGCAT CGTGGTGTCA CGCTCGTCGT TTGGTATGGC TTCATTCAGC
     5521 TCCGGTTCCC AACGATCAAG GCGAGTTACA TGATCCCCCA TGTTGTGCAA AAAAGCGGTT
     5581 AGCTCCTTCG GTCCTCCGAT CGTTGTCAGA AGTAAGTTGG CCGCAGTGTT ATCACTCATG
     5641 GTTATGGCAG CACTGCATAA TTCTCTTACT GTCATGCCAT CCGTAAGATG CTTTTCTGTG
     5701 ACTGGTGAGT ACTCAACCAA GTCATTCTGA GAATAGTGTA TGCGGCGACC GAGTTGCTCT
     5761 TGCCCGGCGT CAATACGGGA TAATACCGCG CCACATAGCA GAACTTTAAA AGTGCTCATC
     5821 ATTGGAAAAC GTTCTTCGGG GCGAAAACTC TCAAGGATCT TACCGCTGTT GAGATCCAGT
     5881 TCGATGTAAC CCACTCGTGC ACCCAACTGA TCTTCAGCAT CTTTTACTTT CACCAGCGTT
     5941 TCTGGGTGAG CAAAAACAGG AAGGCAAAAT GCCGCAAAAA AGGGAATAAG GGCGACACGG
     6001 AAATGTTGAA TACTCATACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT
     6061 TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG
     6121 CGCACGAATT GGCCAGCGCT GCCATTTTTG GGGTGAGGCC GTTCGCGGCC GAGGGGCGCA
     6181 GCCCCTGGGG GGATGGGAGG CCCGCGTTAG CGGGCCGGGA GGGTTCGAGA AGGGGGGGCA
     6241 CCCCCCTTCG GCGTGCGCGG TCACGCGCAC AGGGCGCAGC CCTGGTTAAA AACAAGGTTT
     6301 ATAAATATTG GTTTAAAAGC AGGTTAAAAG ACAGGTTAGC GGTGGCCGAA AAACGGGCGG
     6361 AAACCCTTGC AAATGCTGGA TTTTCTGCCT GTGGACAGCC CCTCAAATGT CAATAGGTGC
     6421 GCCCCTCATC TGTCAGCACT CTGCCCCTCA AGTGTCAAGG ATCGCGCCCC TCATCTGTCA
     6481 GTAGTCGCGC CCCTCAAGTG TCAATACCGC AGGGCACTTA TCCCCAGGCT TGTCCACATC
     6541 ATCTGTGGGA AACTCGCGTA AAATCAGGCG TTTTCGCCGA TTTGCGAGGC TGGCCAGCTC
     6601 CACGTCGCCG GCCGAAATCG AGCCTGCCCC TCATCTGTCA ACGCCGCGCC GGGTGAGTCG
     6661 GCCCCTCAAG TGTCAACGTC CGCCCCTCAT CTGTCAGTGA GGGCCAAGTT TTCCGCGAGG
     6721 TATCCACAAC GCCGGCGGCC GCGGTGTCTC GCACACGGCT TCGACGGCGT TTCTGGCGCG
     6781 TTTGCAGGGC CATAGACGGC CGCCAGCCCA GCGGCGAGGG CAACCAGCCC GGTGAGCGTC
//