LOCUS       pEPQD1CB0769_(P5_35Ssho 6213 bp ds-DNA     circular     12-OCT-2020
DEFINITION  .
FEATURES             Location/Qualifiers
     Misc._feature   22..169
                     /label="LB"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    complement(92..116)
                     /label="LB T-DNA repeat"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     Promoter        225..650
                     /label="CaMV 35S promoter short"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     None            228..553
                     /label="CaMV 35S promoter repeat unit"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     None            655..735
                     /label="TMV omega (two 3' bp removed)"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     None            741..1850
                     /label="CrLAMT loganic acid O-methyltransferase CDS, Catharanthus roseus, EU057974 (domesticated, 1 BbsI and 1 SapI site removed)"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     Terminator      1858..2061
                     /label="35S ter"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    complement(2089..2214)
                     /label="RB"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    complement(2107..2131)
                     /label="RB T-DNA repeat"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     CDS             complement(2287..3435)
                     /label="Plasmid replication initiator protein TrfA, P07676 (TRFA_ECOLX)"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     rep_origin      complement(3771..4359)
                     /label="pBR322 origin of replication (derivative of pMB1/ColE1)"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     primer_bind     complement(3816..3834)
                     /label="QMD062 primer"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     primer_bind     complement(3851..3870)
                     /label="pBR322ori-F"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     CDS             complement(4530..5390)
                     /label="beta-lactamase, ampicillin resistance (ampR)"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     rep_origin      complement(5582..6213)
                     /label="RK2 (OriV) origin of replication (incP incompatibility group)"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
ORIGIN
        1 GCAAAGGAGA TCCTGATCTG ACTGATGGGC TGCCTGTATC GAGTGGTGAT TTTGTGCCGA
       61 GCTGCCGGTC GGGGAGCTGT TGGCTGGCTG GTGGCAGGAT ATATTGTGGT GTAAACAAAT
      121 TGACGCTTAG ACAACTTAAT AACACATTGC GGACGTTTTT AATGTACTGG GGTGGATGCA
      181 GTGGGCCCCA CTCTGTGAAG ACAACAGAGA ATTCGCATGC GGAGGTCAAC ATGGTGGAGC
      241 ACGACACTCT GGTCTACTCC AAAAATGTCA AAGATACAGT CTCAGAAGAT CAAAGGGCTA
      301 TTGAGACTTT TCAACAAAGG ATAATTTCGG GAAACCTCCT CGGATTCCAT TGCCCAGCTA
      361 TCTGTCACTT CATCGAAAGG ACAGTAGAAA AGGAAGGTGG CTCCTACAAA TGCCATCATT
      421 GCGATAAAGG AAAGGCTATC ATTCAAGATC TCTCTGCCGA CAGTGGTCCC AAAGATGGAC
      481 CCCCACCCAC GAGGAGCATC GTGGAAAAAG AAGAGGTTCC AACCACGTCT ACAAAGCAAG
      541 TGGATTGATG TGACATCTCC ACTGACGTAA GGGATGACGC ACAATCCCAC TATCCTTCGC
      601 AAGACCCTTC CTCTATATAA GGAAGTTCAT TTCATTTGGA GAGGACACGC TCGAGTATAA
      661 GAGCTCATTT TTACAACAAT TACCAACAAC AACAAACAAC AAACAACATT ACAATTACAT
      721 TTACAATTAT CGATACAATG GTTGCCACAA TTGATTCCAT TGAAATGCCT GCACTTCCAA
      781 CAGCTGTGGA AGCCCACCCA ATGAAAGGTG GTGATGACTC CCACAGTTAC TCCCAAAATT
      841 CTTGCTACCA GAAAGGAGTA ATTGATGCAG CCAAGGCAGT GATTGTTGAA GCAGTGAATG
      901 AAAAATTGGA TCTTGAAAAT AATCCAATCT TTGATCCAAT CAAACCTTTC CGCATTGCAG
      961 ATTTTGGATG TTCAACAGGA CCAAATACTT TCCACGCTAT GCAAAACATA GTAGAATCAG
     1021 TTGAAACAAA ATACAAATCA CTTCAAAAAA CCCCTGAATT CCATGTTTTC TTCAATGATC
     1081 ATGTTAACAA TGATTTCAAT GTTCTCTTTA GATCCCTTCC ACCAAACAGG GAATTTTTTG
     1141 CTGCTGGTGT TCCTGGATCT TTCTACACTA GAGTTTTTCC CAAAAATAGC ATTCATTTTG
     1201 CTCATTGTTC TTATGCACTT CATTGGTTAT CTAAAGTGCC CAAGGAAATT CAAGATAAAA
     1261 ATTCTTTGGC TTATAATAAG GGAAGAATTC ATTACACTGG TACTGAAAAA CATGTTGTTA
     1321 AGGCTTATTT TGGTCAATTC CAAAGAGATT TTGAAGGGTT TTTAAAAGCA AGAGCTCAAG
     1381 AAATTGTTGT TGGAGGGTTG ATGGTGATTC AAATACCTGG GCTTCCTAGT GGTGAAGTCC
     1441 TTTTCTCAAG GACTGGTGCT GGTTTGCTTC ACTTCCTTTT GGGAACTTCC TTGATGGAAT
     1501 TGGTTAACAA GGGAATCATC AATGAAGAAA GTGTAGATTC CTTCAATTTG CCTCAATATC
     1561 ATCCCTCAGT TGAAGATTTG GAAATGGTGA TAGAAATGAA CGATTGTTTC ACAATTGAAA
     1621 GGGTTGGAAC TTTACCTCAT CCCATGAAGA ATTTACCATT TGATGTTCAA AGGACTTCTT
     1681 TACAAGTAAG AGCAATCATG GAATGCATTC TCACTGAACA TTTTGGGGAA AATATTTTGG
     1741 ATCCATTATT TGAAATCTAC ACCAAAAATT TGCAAGAGAA TTTCCATGTT TTTGATAAGG
     1801 AAATTAGAAA GGATGCAGAT TTGTACCTTG TCTTGAAACG CAAGGGAAAT TAAGCTTCTC
     1861 TAGCTAGAGT CGATCGACAA GCTCGAGTTT CTCCATAATA ATGTGTGAGT AGTTCCCAGA
     1921 TAAGGGAATT AGGGTTCCTA TAGGGTTTCG CTCATGTGTT GAGCATATAA GAAACCCTTA
     1981 GTATGTATTT GTATTTGTAA AATACTTCTA TCAATAAAAT TTCTAATTCC TAAAACCAAA
     2041 ATCCAGTACT AAAATCCAGA TCGCTTGTGT TGTCTTCTGC ACGAAGTGGT TTAAACTATC
     2101 AGTGTTTGAC AGGATATATT GGCGGGTAAA CCTAAGAGAA AAGAGCGTTT ATTAGAATAA
     2161 TCGGATATTT AAAAGGGCGT GAAAAGGTTT ATCCGTTCGT CCATTTGTAT GTGCATGCCA
     2221 ACCACAGGGT TCCCCAGATC AGGCGCTGGC TGCTGAACCC CCAGCCGGAA CTGACCCCAC
     2281 AAGGCCCTAG CGTTTGCAAT GCACCAGGTC ATCATTGACC CAGGCGTGTT CCACCAGGCC
     2341 GCTGCCTCGC AACTCTTCGC AGGCTTCGCC GACCTGCTCG CGCCACTTCT TCACGCGGGT
     2401 GGAATCCGAT CCGCACATGA GGCGGAAGGT TTCCAGCTTG AGCGGGTACG GCTCCCGGTG
     2461 CGAGCTGAAA TAGTCGAACA TCCGTCGGGC CGTCGGCGAC AGCTTGCGGT ACTTCTCCCA
     2521 TATGAATTTC GTGTAGTGGT CGCCAGCAAA CAGCACGACG ATTTCCTCGT CGATCAGGAC
     2581 CTGGCAACGG GACGTTTTCT TGCCACGGTC CAGGACGCGG AAGCGGTGCA GCAGCGACAC
     2641 CGATTCCAGG TGCCCAACGC GGTCGGACGT GAAGCCCATC GCCGTCGCCT GTAGGCGCGA
     2701 CAGGCATTCC TCGGCCTTCG TGTAATACCG GCCATTGATC GACCAGCCCA GGTCCTGGCA
     2761 AAGCTCGTAG AACGTGAAGG TGATCGGCTC GCCGATAGGG GTGCGCTTCG CGTACTCCAA
     2821 CACCTGCTGC CACACCAGTT CGTCATCGTC GGCCCGCAGC TCGACGCCGG TGTAGGTGAT
     2881 CTTCACGTCC TTGTTGACGT GGAAAATGAC CTTGTTTTGC AGCGCCTCGC GCGGGATTTT
     2941 CTTGTTGCGC GTGGTGAACA GGGCAGAGCG GGCCGTGTCG TTTGGCATCG CTCGCATCGT
     3001 GTCCGGCCAC GGCGCAATAT CGAACAAGGA AAGCTGCATT TCCTTGATCT GCTGCTTCGT
     3061 GTGTTTCAGC AACGCGGCCT GCTTGGCCTC GCTGACCTGT TTTGCCAGGT CCTCGCCGGC
     3121 GGTTTTTCGC TTCTTGGTCG TCATAGTTCC TCGCGTGTCG ATGGTCATCG ACTTCGCCAA
     3181 ACCTGCCGCC TCCTGTTCAA GACGACGCGA ACGCTCCACG GCGGCCGATG GCGCGGGCAG
     3241 GGCAGGGGGA GCCAGTTGCA CGCTGTCGCG CTCGATCTTG GCCGTAGCTT GCTGGACCAT
     3301 CGAGCCGACG GACTGGAAGG TTTCGCGGGG CGCACGCATG ACGGTGCGGC TTGCGATGGT
     3361 TTCGGCATCC TCGGCGGAAA ACCCCGCGTC GATCAGTTCT TGCCTGTATG CCTTCCGGTC
     3421 AAACGTCCGA TTCATTCACC CTCCTTGCGG GATTGCCCCG ACTCACGCCG GGGCAATGTG
     3481 CCCTTATTCC TGATTTGACC CGCCTGGTGC CTTGGTGTCC AGATAATCCA CCTTATCGGC
     3541 AATGAAGTCG GTCCCGTAGA CCGTCTGGCC GTCCTTCTCG TACTTGGTAT TCCGAATCTT
     3601 GCCCTGCACG AATACCAGCG ACCCCTTGCC CAAATACTTG CCGTGGGCCT CGGCCTGAGA
     3661 GCCAAAACAC TTGATGCGGA AGAAGTCGGT GCGCTCCTGC TTGTCGCCGG CATCGTTGCG
     3721 CCACATCTAG GATCTGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG
     3781 CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG
     3841 ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT
     3901 CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT
     3961 TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC
     4021 TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT
     4081 GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT
     4141 AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC
     4201 TACACTAGAA GGACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA
     4261 AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT
     4321 TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT
     4381 ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA
     4441 TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA
     4501 AGTATATATG AGTAAACTTG GTCTGACAGT TACCAATGCT TAATCAGTGA GGCACCTATC
     4561 TCAGCGATCT GTCTATTTCG TTCATCCATA GTTGCCTGAC TCCCCGTCGT GTAGATAACT
     4621 ACGATACGGG AGGGCTTACC ATCTGGCCCC AGTGCTGCAA TGATACCGCG AGAACCACGC
     4681 TCACCGGCTC CAGATTTATC AGCAATAAAC CAGCCAGCCG GAAGGGCCGA GCGCAGAAGT
     4741 GGTCCTGCAA CTTTATCCGC CTCCATCCAG TCTATTAATT GTTGCCGGGA AGCTAGAGTA
     4801 AGTAGTTCGC CAGTTAATAG TTTGCGCAAC GTTGTTGCCA TTGCTACAGG CATCGTGGTG
     4861 TCACGCTCGT CGTTTGGTAT GGCTTCATTC AGCTCCGGTT CCCAACGATC AAGGCGAGTT
     4921 ACATGATCCC CCATGTTGTG CAAAAAAGCG GTTAGCTCCT TCGGTCCTCC GATCGTTGTC
     4981 AGAAGTAAGT TGGCCGCAGT GTTATCACTC ATGGTTATGG CAGCACTGCA TAATTCTCTT
     5041 ACTGTCATGC CATCCGTAAG ATGCTTTTCT GTGACTGGTG AGTACTCAAC CAAGTCATTC
     5101 TGAGAATAGT GTATGCGGCG ACCGAGTTGC TCTTGCCCGG CGTCAATACG GGATAATACC
     5161 GCGCCACATA GCAGAACTTT AAAAGTGCTC ATCATTGGAA AACGTTCTTC GGGGCGAAAA
     5221 CTCTCAAGGA TCTTACCGCT GTTGAGATCC AGTTCGATGT AACCCACTCG TGCACCCAAC
     5281 TGATCTTCAG CATCTTTTAC TTTCACCAGC GTTTCTGGGT GAGCAAAAAC AGGAAGGCAA
     5341 AATGCCGCAA AAAAGGGAAT AAGGGCGACA CGGAAATGTT GAATACTCAT ACTCTTCCTT
     5401 TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA
     5461 TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACGA ATTGGCCAGC GCTGCCATTT
     5521 TTGGGGTGAG GCCGTTCGCG GCCGAGGGGC GCAGCCCCTG GGGGGATGGG AGGCCCGCGT
     5581 TAGCGGGCCG GGAGGGTTCG AGAAGGGGGG GCACCCCCCT TCGGCGTGCG CGGTCACGCG
     5641 CACAGGGCGC AGCCCTGGTT AAAAACAAGG TTTATAAATA TTGGTTTAAA AGCAGGTTAA
     5701 AAGACAGGTT AGCGGTGGCC GAAAAACGGG CGGAAACCCT TGCAAATGCT GGATTTTCTG
     5761 CCTGTGGACA GCCCCTCAAA TGTCAATAGG TGCGCCCCTC ATCTGTCAGC ACTCTGCCCC
     5821 TCAAGTGTCA AGGATCGCGC CCCTCATCTG TCAGTAGTCG CGCCCCTCAA GTGTCAATAC
     5881 CGCAGGGCAC TTATCCCCAG GCTTGTCCAC ATCATCTGTG GGAAACTCGC GTAAAATCAG
     5941 GCGTTTTCGC CGATTTGCGA GGCTGGCCAG CTCCACGTCG CCGGCCGAAA TCGAGCCTGC
     6001 CCCTCATCTG TCAACGCCGC GCCGGGTGAG TCGGCCCCTC AAGTGTCAAC GTCCGCCCCT
     6061 CATCTGTCAG TGAGGGCCAA GTTTTCCGCG AGGTATCCAC AACGCCGGCG GCCGCGGTGT
     6121 CTCGCACACG GCTTCGACGG CGTTTCTGGC GCGTTTGCAG GGCCATAGAC GGCCGCCAGC
     6181 CCAGCGGCGA GGGCAACCAG CCCGGTGAGC GTC
//