LOCUS       pEPQD1CB0771_(P6_35Ssho 6681 bp ds-DNA     circular     12-OCT-2020
DEFINITION  .
FEATURES             Location/Qualifiers
     Misc._feature   22..169
                     /label="LB"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    complement(92..116)
                     /label="LB T-DNA repeat"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     Promoter        225..650
                     /label="CaMV 35S promoter short"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     None            228..553
                     /label="CaMV 35S promoter repeat unit"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     None            655..735
                     /label="TMV omega (two 3' bp removed)"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    741..2318
                     /label="CrSLS secologanin synthase, Catharanthus roseus KF309242.1 (domesticated, 1 SapI site removed))"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    741..2318
                     /label="name depends on ref: SLS1 Brown et al. PNAS 112(11) 3205-3210 (2015), SLS2 de Bernonville et al. BMC Genomics 16(1) 619 (2015)"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     Terminator      2326..2529
                     /label="35S ter"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    complement(2557..2682)
                     /label="RB"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    complement(2575..2599)
                     /label="RB T-DNA repeat"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     CDS             complement(2755..3903)
                     /label="Plasmid replication initiator protein TrfA, P07676 (TRFA_ECOLX)"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     rep_origin      complement(4239..4827)
                     /label="pBR322 origin of replication (derivative of pMB1/ColE1)"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     primer_bind     complement(4284..4302)
                     /label="QMD062 primer"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     primer_bind     complement(4319..4338)
                     /label="pBR322ori-F"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     CDS             complement(4998..5858)
                     /label="beta-lactamase, ampicillin resistance (ampR)"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     rep_origin      complement(6050..6681)
                     /label="RK2 (OriV) origin of replication (incP incompatibility group)"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
ORIGIN
        1 GCAAAGGAGA TCCTGATCTG ACTGATGGGC TGCCTGTATC GAGTGGTGAT TTTGTGCCGA
       61 GCTGCCGGTC GGGGAGCTGT TGGCTGGCTG GTGGCAGGAT ATATTGTGGT GTAAACAAAT
      121 TGACGCTTAG ACAACTTAAT AACACATTGC GGACGTTTTT AATGTACTGG GGTGGATGCA
      181 GTGGGCCCCA CTCTGTGAAG ACAATGTGGA ATTCCTCGAG GGAGGTCAAC ATGGTGGAGC
      241 ACGACACTCT GGTCTACTCC AAAAATGTCA AAGATACAGT CTCAGAAGAT CAAAGGGCTA
      301 TTGAGACTTT TCAACAAAGG ATAATTTCGG GAAACCTCCT CGGATTCCAT TGCCCAGCTA
      361 TCTGTCACTT CATCGAAAGG ACAGTAGAAA AGGAAGGTGG CTCCTACAAA TGCCATCATT
      421 GCGATAAAGG AAAGGCTATC ATTCAAGATC TCTCTGCCGA CAGTGGTCCC AAAGATGGAC
      481 CCCCACCCAC GAGGAGCATC GTGGAAAAAG AAGAGGTTCC AACCACGTCT ACAAAGCAAG
      541 TGGATTGATG TGACATCTCC ACTGACGTAA GGGATGACGC ACAATCCCAC TATCCTTCGC
      601 AAGACCCTTC CTCTATATAA GGAAGTTCAT TTCATTTGGA GAGGACACGC TCGAGTATAA
      661 GAGCTCATTT TTACAACAAT TACCAACAAC AACAAACAAC AAACAACATT ACAATTACAT
      721 TTACAATTAT CGATACAATG GAGATGGATA TGGATATCAT TAGAAAGGCA ATTGCTGCCA
      781 CTATCTTTGC ATTGGTTATG GCTTGGGCGT GGAGAGTGTT GGATTGGGCA TGGTTTACTC
      841 CTAAGAGGAT CGAGAAACGT CTAAGGCAGC AAGGTTTTAG AGGAAATCCT TATAGATTCT
      901 TGGTTGGAGA TGTTAAGGAG AGTGGAAAAA TGCATCAAGA AGCCTTGTCT AACCCCATGG
      961 AGTTCGACAA CGATATTGTT CCTCGCCTCA TGCCACATAT TAACCACACC ATAAAAACTT
     1021 ACGGTAGGAA TTCATTTACA TGGATGGGAA GGATTCCAAG GATTCATGTT ATGGAACCTG
     1081 AACTTATTAA GGAAGTATTG ACCCACTCCA GCAAATACCA AAAGAACTTT GATGTTCACA
     1141 ATCCCCTTGT TAAGTTCCTT CTCACTGGAG TTGGAAGCTT TGAAGGGGCA AAATGGTCCA
     1201 AACACAGAAG AATCATTTCC CCTGCCTTCA CTCTTGAGAA ACTAAAGTCA ATGTTGCCGG
     1261 CTTTTGCCAT ATGCTATCAT GACATGTTGA CGAAATGGGA GAAATTAGCA GAAAAAGAAG
     1321 GATCCCATGA AGTTGATATC TTCCCCACAT TTGATGTTTT GACAAGTGAT GTAATTTCAA
     1381 AGGTTGCATT TGGTAGCACA TACGATGAAG GAGGCAAAAT CTTCAGACTA TTGAAAGAAC
     1441 TCATGGATCT CACAATTGAT TGCATGAGAG ATGTCTACAT CCCAGGGTGG AGCTACTTGC
     1501 CAACCAAGAG GAACAAGAGG ATGAAAGAAA TTAACAAAGA AATCACAGAT ATGCTAAGGT
     1561 TCATCATCAA CAAGAGAATG AAGGCTTTGA AGGCTGGAGA ACCAGGTGAG GATGACTTGT
     1621 TGGGAGTCCT GTTGGAATCA AACATTCAAG AAATTCAGAA ACAAGGAAAC AGGAAGGATG
     1681 GTGGAATGAC AATCAACGAT GTGATTGAGG AATGCAAATT GTTCTACTTT GCTGGTCAAG
     1741 AAACCACCGG AGTTTTGCTC ACATGGACCA CCATTTTATT GAGCAAGCAT CCCGAGTGGC
     1801 AAGAGCGAGC TAGGGAAGAA GTTCTCCAAG CCTTTGGCAA GAACAAGCCT GAATTTGAAC
     1861 GCTTGAATCA TCTTAAATAT GTTTCTATGA TCTTATACGA GGTTCTAAGG TTGTACCCGC
     1921 CAGTTATTGA TCTGACCAAG ATTATTCACG AGGACACAAA GTTAGGGCCG TACACAATCC
     1981 CTGCAGGAAC ACAAGTAATG TTGCCGACAG TAATGCTTCA TAGAGAGAAG TCCATTTGGG
     2041 GAGAAGATGC AATGGAATTC AACCCAATGA GATTTGCTGA TGGAGTTGCC AATGCCACCA
     2101 AGAACAATGT CACATATTTG CCATTCAGTT GGGGACCTAG AGTTTGCCTC GGCCAAAACT
     2161 TTGCACTTCT TCAAGCAAAA TTAGGGTTGG CAATGATTTT ACAACGCTTC AAATTTGATG
     2221 TAGCTCCATC CTATGTTCAT GCTCCCTTTA CCATTCTTAC AGTTCAACCT CAGTTTGGTT
     2281 CTCATGTTAT CTACAAGAAG CTTGAGAGAC AGAATTTTTA AGCTTCTCTA GCTAGAGTCG
     2341 ATCGACAAGC TCGAGTTTCT CCATAATAAT GTGTGAGTAG TTCCCAGATA AGGGAATTAG
     2401 GGTTCCTATA GGGTTTCGCT CATGTGTTGA GCATATAAGA AACCCTTAGT ATGTATTTGT
     2461 ATTTGTAAAA TACTTCTATC AATAAAATTT CTAATTCCTA AAACCAAAAT CCAGTACTAA
     2521 AATCCAGATC GCTGAGCTTG TCTTCTGCAC GAAGTGGTTT AAACTATCAG TGTTTGACAG
     2581 GATATATTGG CGGGTAAACC TAAGAGAAAA GAGCGTTTAT TAGAATAATC GGATATTTAA
     2641 AAGGGCGTGA AAAGGTTTAT CCGTTCGTCC ATTTGTATGT GCATGCCAAC CACAGGGTTC
     2701 CCCAGATCAG GCGCTGGCTG CTGAACCCCC AGCCGGAACT GACCCCACAA GGCCCTAGCG
     2761 TTTGCAATGC ACCAGGTCAT CATTGACCCA GGCGTGTTCC ACCAGGCCGC TGCCTCGCAA
     2821 CTCTTCGCAG GCTTCGCCGA CCTGCTCGCG CCACTTCTTC ACGCGGGTGG AATCCGATCC
     2881 GCACATGAGG CGGAAGGTTT CCAGCTTGAG CGGGTACGGC TCCCGGTGCG AGCTGAAATA
     2941 GTCGAACATC CGTCGGGCCG TCGGCGACAG CTTGCGGTAC TTCTCCCATA TGAATTTCGT
     3001 GTAGTGGTCG CCAGCAAACA GCACGACGAT TTCCTCGTCG ATCAGGACCT GGCAACGGGA
     3061 CGTTTTCTTG CCACGGTCCA GGACGCGGAA GCGGTGCAGC AGCGACACCG ATTCCAGGTG
     3121 CCCAACGCGG TCGGACGTGA AGCCCATCGC CGTCGCCTGT AGGCGCGACA GGCATTCCTC
     3181 GGCCTTCGTG TAATACCGGC CATTGATCGA CCAGCCCAGG TCCTGGCAAA GCTCGTAGAA
     3241 CGTGAAGGTG ATCGGCTCGC CGATAGGGGT GCGCTTCGCG TACTCCAACA CCTGCTGCCA
     3301 CACCAGTTCG TCATCGTCGG CCCGCAGCTC GACGCCGGTG TAGGTGATCT TCACGTCCTT
     3361 GTTGACGTGG AAAATGACCT TGTTTTGCAG CGCCTCGCGC GGGATTTTCT TGTTGCGCGT
     3421 GGTGAACAGG GCAGAGCGGG CCGTGTCGTT TGGCATCGCT CGCATCGTGT CCGGCCACGG
     3481 CGCAATATCG AACAAGGAAA GCTGCATTTC CTTGATCTGC TGCTTCGTGT GTTTCAGCAA
     3541 CGCGGCCTGC TTGGCCTCGC TGACCTGTTT TGCCAGGTCC TCGCCGGCGG TTTTTCGCTT
     3601 CTTGGTCGTC ATAGTTCCTC GCGTGTCGAT GGTCATCGAC TTCGCCAAAC CTGCCGCCTC
     3661 CTGTTCAAGA CGACGCGAAC GCTCCACGGC GGCCGATGGC GCGGGCAGGG CAGGGGGAGC
     3721 CAGTTGCACG CTGTCGCGCT CGATCTTGGC CGTAGCTTGC TGGACCATCG AGCCGACGGA
     3781 CTGGAAGGTT TCGCGGGGCG CACGCATGAC GGTGCGGCTT GCGATGGTTT CGGCATCCTC
     3841 GGCGGAAAAC CCCGCGTCGA TCAGTTCTTG CCTGTATGCC TTCCGGTCAA ACGTCCGATT
     3901 CATTCACCCT CCTTGCGGGA TTGCCCCGAC TCACGCCGGG GCAATGTGCC CTTATTCCTG
     3961 ATTTGACCCG CCTGGTGCCT TGGTGTCCAG ATAATCCACC TTATCGGCAA TGAAGTCGGT
     4021 CCCGTAGACC GTCTGGCCGT CCTTCTCGTA CTTGGTATTC CGAATCTTGC CCTGCACGAA
     4081 TACCAGCGAC CCCTTGCCCA AATACTTGCC GTGGGCCTCG GCCTGAGAGC CAAAACACTT
     4141 GATGCGGAAG AAGTCGGTGC GCTCCTGCTT GTCGCCGGCA TCGTTGCGCC ACATCTAGGA
     4201 TCTGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT
     4261 GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA
     4321 AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG
     4381 CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA
     4441 CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA
     4501 CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG
     4561 GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG
     4621 TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGG
     4681 ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC
     4741 TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG
     4801 ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC
     4861 GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC
     4921 TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG
     4981 TAAACTTGGT CTGACAGTTA CCAATGCTTA ATCAGTGAGG CACCTATCTC AGCGATCTGT
     5041 CTATTTCGTT CATCCATAGT TGCCTGACTC CCCGTCGTGT AGATAACTAC GATACGGGAG
     5101 GGCTTACCAT CTGGCCCCAG TGCTGCAATG ATACCGCGAG AACCACGCTC ACCGGCTCCA
     5161 GATTTATCAG CAATAAACCA GCCAGCCGGA AGGGCCGAGC GCAGAAGTGG TCCTGCAACT
     5221 TTATCCGCCT CCATCCAGTC TATTAATTGT TGCCGGGAAG CTAGAGTAAG TAGTTCGCCA
     5281 GTTAATAGTT TGCGCAACGT TGTTGCCATT GCTACAGGCA TCGTGGTGTC ACGCTCGTCG
     5341 TTTGGTATGG CTTCATTCAG CTCCGGTTCC CAACGATCAA GGCGAGTTAC ATGATCCCCC
     5401 ATGTTGTGCA AAAAAGCGGT TAGCTCCTTC GGTCCTCCGA TCGTTGTCAG AAGTAAGTTG
     5461 GCCGCAGTGT TATCACTCAT GGTTATGGCA GCACTGCATA ATTCTCTTAC TGTCATGCCA
     5521 TCCGTAAGAT GCTTTTCTGT GACTGGTGAG TACTCAACCA AGTCATTCTG AGAATAGTGT
     5581 ATGCGGCGAC CGAGTTGCTC TTGCCCGGCG TCAATACGGG ATAATACCGC GCCACATAGC
     5641 AGAACTTTAA AAGTGCTCAT CATTGGAAAA CGTTCTTCGG GGCGAAAACT CTCAAGGATC
     5701 TTACCGCTGT TGAGATCCAG TTCGATGTAA CCCACTCGTG CACCCAACTG ATCTTCAGCA
     5761 TCTTTTACTT TCACCAGCGT TTCTGGGTGA GCAAAAACAG GAAGGCAAAA TGCCGCAAAA
     5821 AAGGGAATAA GGGCGACACG GAAATGTTGA ATACTCATAC TCTTCCTTTT TCAATATTAT
     5881 TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA
     5941 AATAAACAAA TAGGGGTTCC GCGCACGAAT TGGCCAGCGC TGCCATTTTT GGGGTGAGGC
     6001 CGTTCGCGGC CGAGGGGCGC AGCCCCTGGG GGGATGGGAG GCCCGCGTTA GCGGGCCGGG
     6061 AGGGTTCGAG AAGGGGGGGC ACCCCCCTTC GGCGTGCGCG GTCACGCGCA CAGGGCGCAG
     6121 CCCTGGTTAA AAACAAGGTT TATAAATATT GGTTTAAAAG CAGGTTAAAA GACAGGTTAG
     6181 CGGTGGCCGA AAAACGGGCG GAAACCCTTG CAAATGCTGG ATTTTCTGCC TGTGGACAGC
     6241 CCCTCAAATG TCAATAGGTG CGCCCCTCAT CTGTCAGCAC TCTGCCCCTC AAGTGTCAAG
     6301 GATCGCGCCC CTCATCTGTC AGTAGTCGCG CCCCTCAAGT GTCAATACCG CAGGGCACTT
     6361 ATCCCCAGGC TTGTCCACAT CATCTGTGGG AAACTCGCGT AAAATCAGGC GTTTTCGCCG
     6421 ATTTGCGAGG CTGGCCAGCT CCACGTCGCC GGCCGAAATC GAGCCTGCCC CTCATCTGTC
     6481 AACGCCGCGC CGGGTGAGTC GGCCCCTCAA GTGTCAACGT CCGCCCCTCA TCTGTCAGTG
     6541 AGGGCCAAGT TTTCCGCGAG GTATCCACAA CGCCGGCGGC CGCGGTGTCT CGCACACGGC
     6601 TTCGACGGCG TTTCTGGCGC GTTTGCAGGG CCATAGACGG CCGCCAGCCC AGCGGCGAGG
     6661 GCAACCAGCC CGGTGAGCGT C
//