LOCUS       pEPQD1CB0772_(P6_35Ssho 6858 bp ds-DNA     circular     13-OCT-2020
DEFINITION  .
FEATURES             Location/Qualifiers
     Misc._feature   22..169
                     /label="LB"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    complement(92..116)
                     /label="LB T-DNA repeat"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     Promoter        225..650
                     /label="CaMV 35S promoter short"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     None            228..553
                     /label="CaMV 35S promoter repeat unit"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     Golden_Gate_Par 651..917
                     /label="5U-TMV+SP-chloroplast"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     5'_UTR          655..737
                     /label="TMV omega"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     Transit_peptide 738..913
                     /label="chloroplast targeting signal"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    918..2495
                     /label="CrSLS secologanin synthase, Catharanthus roseus KF309242.1 (domesticated, 1 SapI site removed))"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    918..2495
                     /label="name depends on ref: SLS1 Brown et al. PNAS 112(11) 3205-3210 (2015), SLS2 de Bernonville et al. BMC Genomics 16(1) 619 (2015)"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     Terminator      2503..2706
                     /label="35S ter"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    complement(2734..2859)
                     /label="RB"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    complement(2752..2776)
                     /label="RB T-DNA repeat"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     CDS             complement(2932..4080)
                     /label="Plasmid replication initiator protein TrfA, P07676 (TRFA_ECOLX)"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     rep_origin      complement(4416..5004)
                     /label="pBR322 origin of replication (derivative of pMB1/ColE1)"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     primer_bind     complement(4461..4479)
                     /label="QMD062 primer"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     primer_bind     complement(4496..4515)
                     /label="pBR322ori-F"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     CDS             complement(5175..6035)
                     /label="beta-lactamase, ampicillin resistance (ampR)"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     rep_origin      complement(6227..6858)
                     /label="RK2 (OriV) origin of replication (incP incompatibility group)"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
ORIGIN
        1 GCAAAGGAGA TCCTGATCTG ACTGATGGGC TGCCTGTATC GAGTGGTGAT TTTGTGCCGA
       61 GCTGCCGGTC GGGGAGCTGT TGGCTGGCTG GTGGCAGGAT ATATTGTGGT GTAAACAAAT
      121 TGACGCTTAG ACAACTTAAT AACACATTGC GGACGTTTTT AATGTACTGG GGTGGATGCA
      181 GTGGGCCCCA CTCTGTGAAG ACAATGTGGA ATTCCTCGAG GGAGGTCAAC ATGGTGGAGC
      241 ACGACACTCT GGTCTACTCC AAAAATGTCA AAGATACAGT CTCAGAAGAT CAAAGGGCTA
      301 TTGAGACTTT TCAACAAAGG ATAATTTCGG GAAACCTCCT CGGATTCCAT TGCCCAGCTA
      361 TCTGTCACTT CATCGAAAGG ACAGTAGAAA AGGAAGGTGG CTCCTACAAA TGCCATCATT
      421 GCGATAAAGG AAAGGCTATC ATTCAAGATC TCTCTGCCGA CAGTGGTCCC AAAGATGGAC
      481 CCCCACCCAC GAGGAGCATC GTGGAAAAAG AAGAGGTTCC AACCACGTCT ACAAAGCAAG
      541 TGGATTGATG TGACATCTCC ACTGACGTAA GGGATGACGC ACAATCCCAC TATCCTTCGC
      601 AAGACCCTTC CTCTATATAA GGAAGTTCAT TTCATTTGGA GAGGACACGC TACTGTATAA
      661 GAGCTCATTT TTACAACAAT TACCAACAAC AACAAACAAC AAACAACATT ACAATTACAT
      721 TTACAATTAT CGATAACATG GCTTCTTCTA TGCTTTCTTC TGCTGCTGTT GTTGCTACTC
      781 GTGCTAGTGC TGCTCAAGCT AGTATGGTTG CTCCTTTTAC TGGACTTAAG TCTGCTGCTT
      841 CTTTTCCTGT TACTAGAAAG CAAAACAACC TTGATATTAC TTCTATTGCT AGTAACGGAG
      901 GAAGAGTTCG AGCAATGGAG ATGGATATGG ATATCATTAG AAAGGCAATT GCTGCCACTA
      961 TCTTTGCATT GGTTATGGCT TGGGCGTGGA GAGTGTTGGA TTGGGCATGG TTTACTCCTA
     1021 AGAGGATCGA GAAACGTCTA AGGCAGCAAG GTTTTAGAGG AAATCCTTAT AGATTCTTGG
     1081 TTGGAGATGT TAAGGAGAGT GGAAAAATGC ATCAAGAAGC CTTGTCTAAC CCCATGGAGT
     1141 TCGACAACGA TATTGTTCCT CGCCTCATGC CACATATTAA CCACACCATA AAAACTTACG
     1201 GTAGGAATTC ATTTACATGG ATGGGAAGGA TTCCAAGGAT TCATGTTATG GAACCTGAAC
     1261 TTATTAAGGA AGTATTGACC CACTCCAGCA AATACCAAAA GAACTTTGAT GTTCACAATC
     1321 CCCTTGTTAA GTTCCTTCTC ACTGGAGTTG GAAGCTTTGA AGGGGCAAAA TGGTCCAAAC
     1381 ACAGAAGAAT CATTTCCCCT GCCTTCACTC TTGAGAAACT AAAGTCAATG TTGCCGGCTT
     1441 TTGCCATATG CTATCATGAC ATGTTGACGA AATGGGAGAA ATTAGCAGAA AAAGAAGGAT
     1501 CCCATGAAGT TGATATCTTC CCCACATTTG ATGTTTTGAC AAGTGATGTA ATTTCAAAGG
     1561 TTGCATTTGG TAGCACATAC GATGAAGGAG GCAAAATCTT CAGACTATTG AAAGAACTCA
     1621 TGGATCTCAC AATTGATTGC ATGAGAGATG TCTACATCCC AGGGTGGAGC TACTTGCCAA
     1681 CCAAGAGGAA CAAGAGGATG AAAGAAATTA ACAAAGAAAT CACAGATATG CTAAGGTTCA
     1741 TCATCAACAA GAGAATGAAG GCTTTGAAGG CTGGAGAACC AGGTGAGGAT GACTTGTTGG
     1801 GAGTCCTGTT GGAATCAAAC ATTCAAGAAA TTCAGAAACA AGGAAACAGG AAGGATGGTG
     1861 GAATGACAAT CAACGATGTG ATTGAGGAAT GCAAATTGTT CTACTTTGCT GGTCAAGAAA
     1921 CCACCGGAGT TTTGCTCACA TGGACCACCA TTTTATTGAG CAAGCATCCC GAGTGGCAAG
     1981 AGCGAGCTAG GGAAGAAGTT CTCCAAGCCT TTGGCAAGAA CAAGCCTGAA TTTGAACGCT
     2041 TGAATCATCT TAAATATGTT TCTATGATCT TATACGAGGT TCTAAGGTTG TACCCGCCAG
     2101 TTATTGATCT GACCAAGATT ATTCACGAGG ACACAAAGTT AGGGCCGTAC ACAATCCCTG
     2161 CAGGAACACA AGTAATGTTG CCGACAGTAA TGCTTCATAG AGAGAAGTCC ATTTGGGGAG
     2221 AAGATGCAAT GGAATTCAAC CCAATGAGAT TTGCTGATGG AGTTGCCAAT GCCACCAAGA
     2281 ACAATGTCAC ATATTTGCCA TTCAGTTGGG GACCTAGAGT TTGCCTCGGC CAAAACTTTG
     2341 CACTTCTTCA AGCAAAATTA GGGTTGGCAA TGATTTTACA ACGCTTCAAA TTTGATGTAG
     2401 CTCCATCCTA TGTTCATGCT CCCTTTACCA TTCTTACAGT TCAACCTCAG TTTGGTTCTC
     2461 ATGTTATCTA CAAGAAGCTT GAGAGACAGA ATTTTTAAGC TTCTCTAGCT AGAGTCGATC
     2521 GACAAGCTCG AGTTTCTCCA TAATAATGTG TGAGTAGTTC CCAGATAAGG GAATTAGGGT
     2581 TCCTATAGGG TTTCGCTCAT GTGTTGAGCA TATAAGAAAC CCTTAGTATG TATTTGTATT
     2641 TGTAAAATAC TTCTATCAAT AAAATTTCTA ATTCCTAAAA CCAAAATCCA GTACTAAAAT
     2701 CCAGATCGCT GAGCTTGTCT TCTGCACGAA GTGGTTTAAA CTATCAGTGT TTGACAGGAT
     2761 ATATTGGCGG GTAAACCTAA GAGAAAAGAG CGTTTATTAG AATAATCGGA TATTTAAAAG
     2821 GGCGTGAAAA GGTTTATCCG TTCGTCCATT TGTATGTGCA TGCCAACCAC AGGGTTCCCC
     2881 AGATCAGGCG CTGGCTGCTG AACCCCCAGC CGGAACTGAC CCCACAAGGC CCTAGCGTTT
     2941 GCAATGCACC AGGTCATCAT TGACCCAGGC GTGTTCCACC AGGCCGCTGC CTCGCAACTC
     3001 TTCGCAGGCT TCGCCGACCT GCTCGCGCCA CTTCTTCACG CGGGTGGAAT CCGATCCGCA
     3061 CATGAGGCGG AAGGTTTCCA GCTTGAGCGG GTACGGCTCC CGGTGCGAGC TGAAATAGTC
     3121 GAACATCCGT CGGGCCGTCG GCGACAGCTT GCGGTACTTC TCCCATATGA ATTTCGTGTA
     3181 GTGGTCGCCA GCAAACAGCA CGACGATTTC CTCGTCGATC AGGACCTGGC AACGGGACGT
     3241 TTTCTTGCCA CGGTCCAGGA CGCGGAAGCG GTGCAGCAGC GACACCGATT CCAGGTGCCC
     3301 AACGCGGTCG GACGTGAAGC CCATCGCCGT CGCCTGTAGG CGCGACAGGC ATTCCTCGGC
     3361 CTTCGTGTAA TACCGGCCAT TGATCGACCA GCCCAGGTCC TGGCAAAGCT CGTAGAACGT
     3421 GAAGGTGATC GGCTCGCCGA TAGGGGTGCG CTTCGCGTAC TCCAACACCT GCTGCCACAC
     3481 CAGTTCGTCA TCGTCGGCCC GCAGCTCGAC GCCGGTGTAG GTGATCTTCA CGTCCTTGTT
     3541 GACGTGGAAA ATGACCTTGT TTTGCAGCGC CTCGCGCGGG ATTTTCTTGT TGCGCGTGGT
     3601 GAACAGGGCA GAGCGGGCCG TGTCGTTTGG CATCGCTCGC ATCGTGTCCG GCCACGGCGC
     3661 AATATCGAAC AAGGAAAGCT GCATTTCCTT GATCTGCTGC TTCGTGTGTT TCAGCAACGC
     3721 GGCCTGCTTG GCCTCGCTGA CCTGTTTTGC CAGGTCCTCG CCGGCGGTTT TTCGCTTCTT
     3781 GGTCGTCATA GTTCCTCGCG TGTCGATGGT CATCGACTTC GCCAAACCTG CCGCCTCCTG
     3841 TTCAAGACGA CGCGAACGCT CCACGGCGGC CGATGGCGCG GGCAGGGCAG GGGGAGCCAG
     3901 TTGCACGCTG TCGCGCTCGA TCTTGGCCGT AGCTTGCTGG ACCATCGAGC CGACGGACTG
     3961 GAAGGTTTCG CGGGGCGCAC GCATGACGGT GCGGCTTGCG ATGGTTTCGG CATCCTCGGC
     4021 GGAAAACCCC GCGTCGATCA GTTCTTGCCT GTATGCCTTC CGGTCAAACG TCCGATTCAT
     4081 TCACCCTCCT TGCGGGATTG CCCCGACTCA CGCCGGGGCA ATGTGCCCTT ATTCCTGATT
     4141 TGACCCGCCT GGTGCCTTGG TGTCCAGATA ATCCACCTTA TCGGCAATGA AGTCGGTCCC
     4201 GTAGACCGTC TGGCCGTCCT TCTCGTACTT GGTATTCCGA ATCTTGCCCT GCACGAATAC
     4261 CAGCGACCCC TTGCCCAAAT ACTTGCCGTG GGCCTCGGCC TGAGAGCCAA AACACTTGAT
     4321 GCGGAAGAAG TCGGTGCGCT CCTGCTTGTC GCCGGCATCG TTGCGCCACA TCTAGGATCT
     4381 GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC
     4441 GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA
     4501 TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT
     4561 ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC
     4621 TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC
     4681 CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA
     4741 AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT
     4801 GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGGACA
     4861 GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT
     4921 TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT
     4981 ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT
     5041 CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC
     5101 ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA
     5161 ACTTGGTCTG ACAGTTACCA ATGCTTAATC AGTGAGGCAC CTATCTCAGC GATCTGTCTA
     5221 TTTCGTTCAT CCATAGTTGC CTGACTCCCC GTCGTGTAGA TAACTACGAT ACGGGAGGGC
     5281 TTACCATCTG GCCCCAGTGC TGCAATGATA CCGCGAGAAC CACGCTCACC GGCTCCAGAT
     5341 TTATCAGCAA TAAACCAGCC AGCCGGAAGG GCCGAGCGCA GAAGTGGTCC TGCAACTTTA
     5401 TCCGCCTCCA TCCAGTCTAT TAATTGTTGC CGGGAAGCTA GAGTAAGTAG TTCGCCAGTT
     5461 AATAGTTTGC GCAACGTTGT TGCCATTGCT ACAGGCATCG TGGTGTCACG CTCGTCGTTT
     5521 GGTATGGCTT CATTCAGCTC CGGTTCCCAA CGATCAAGGC GAGTTACATG ATCCCCCATG
     5581 TTGTGCAAAA AAGCGGTTAG CTCCTTCGGT CCTCCGATCG TTGTCAGAAG TAAGTTGGCC
     5641 GCAGTGTTAT CACTCATGGT TATGGCAGCA CTGCATAATT CTCTTACTGT CATGCCATCC
     5701 GTAAGATGCT TTTCTGTGAC TGGTGAGTAC TCAACCAAGT CATTCTGAGA ATAGTGTATG
     5761 CGGCGACCGA GTTGCTCTTG CCCGGCGTCA ATACGGGATA ATACCGCGCC ACATAGCAGA
     5821 ACTTTAAAAG TGCTCATCAT TGGAAAACGT TCTTCGGGGC GAAAACTCTC AAGGATCTTA
     5881 CCGCTGTTGA GATCCAGTTC GATGTAACCC ACTCGTGCAC CCAACTGATC TTCAGCATCT
     5941 TTTACTTTCA CCAGCGTTTC TGGGTGAGCA AAAACAGGAA GGCAAAATGC CGCAAAAAAG
     6001 GGAATAAGGG CGACACGGAA ATGTTGAATA CTCATACTCT TCCTTTTTCA ATATTATTGA
     6061 AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT
     6121 AAACAAATAG GGGTTCCGCG CACGAATTGG CCAGCGCTGC CATTTTTGGG GTGAGGCCGT
     6181 TCGCGGCCGA GGGGCGCAGC CCCTGGGGGG ATGGGAGGCC CGCGTTAGCG GGCCGGGAGG
     6241 GTTCGAGAAG GGGGGGCACC CCCCTTCGGC GTGCGCGGTC ACGCGCACAG GGCGCAGCCC
     6301 TGGTTAAAAA CAAGGTTTAT AAATATTGGT TTAAAAGCAG GTTAAAAGAC AGGTTAGCGG
     6361 TGGCCGAAAA ACGGGCGGAA ACCCTTGCAA ATGCTGGATT TTCTGCCTGT GGACAGCCCC
     6421 TCAAATGTCA ATAGGTGCGC CCCTCATCTG TCAGCACTCT GCCCCTCAAG TGTCAAGGAT
     6481 CGCGCCCCTC ATCTGTCAGT AGTCGCGCCC CTCAAGTGTC AATACCGCAG GGCACTTATC
     6541 CCCAGGCTTG TCCACATCAT CTGTGGGAAA CTCGCGTAAA ATCAGGCGTT TTCGCCGATT
     6601 TGCGAGGCTG GCCAGCTCCA CGTCGCCGGC CGAAATCGAG CCTGCCCCTC ATCTGTCAAC
     6661 GCCGCGCCGG GTGAGTCGGC CCCTCAAGTG TCAACGTCCG CCCCTCATCT GTCAGTGAGG
     6721 GCCAAGTTTT CCGCGAGGTA TCCACAACGC CGGCGGCCGC GGTGTCTCGC ACACGGCTTC
     6781 GACGGCGTTT CTGGCGCGTT TGCAGGGCCA TAGACGGCCG CCAGCCCAGC GGCGAGGGCA
     6841 ACCAGCCCGG TGAGCGTC
//