LOCUS       pEPQD1CB0773_(P1_35Ssho 6600 bp ds-DNA     circular     13-OCT-2020
DEFINITION  .
FEATURES             Location/Qualifiers
     Misc._feature   22..169
                     /label="LB"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    complement(92..116)
                     /label="LB T-DNA repeat"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     Promoter        225..650
                     /label="CaMV 35S promoter short"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     None            228..553
                     /label="CaMV 35S promoter repeat unit"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     None            655..735
                     /label="TMV omega (two 3' bp removed)"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     None            741..2237
                     /label="CrTDC tryptophan decarboxylase CDS, Catharanthus roseus, M25151 (domesticated, 1 BtgZI site removed)"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     Terminator      2245..2448
                     /label="35S ter"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    complement(2476..2601)
                     /label="RB"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    complement(2494..2518)
                     /label="RB T-DNA repeat"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     CDS             complement(2674..3822)
                     /label="Plasmid replication initiator protein TrfA, P07676 (TRFA_ECOLX)"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     rep_origin      complement(4158..4746)
                     /label="pUC origin of replication (derivative of pBR322/pMB1/ColE1)"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     primer_bind     complement(4203..4221)
                     /label="QMD062 primer"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     primer_bind     complement(4238..4257)
                     /label="pBR322ori-F"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     CDS             complement(4917..5777)
                     /label="beta-lactamase, ampicillin resistance (ampR)"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     promoter        complement(5778..5882)
                     /label="AmpR promoter"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     rep_origin      complement(5969..6600)
                     /label="RK2 (OriV) origin of replication (incP incompatibility group)"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
ORIGIN
        1 GCAAAGGAGA TCCTGATCTG ACTGATGGGC TGCCTGTATC GAGTGGTGAT TTTGTGCCGA
       61 GCTGCCGGTC GGGGAGCTGT TGGCTGGCTG GTGGCAGGAT ATATTGTGGT GTAAACAAAT
      121 TGACGCTTAG ACAACTTAAT AACACATTGC GGACGTTTTT AATGTACTGG GGTGGATGCA
      181 GTGGGCCCCA CTCTGTGAAG ACAATGCCGA ATTCGGATCC GGAGGTCAAC ATGGTGGAGC
      241 ACGACACTCT GGTCTACTCC AAAAATGTCA AAGATACAGT CTCAGAAGAT CAAAGGGCTA
      301 TTGAGACTTT TCAACAAAGG ATAATTTCGG GAAACCTCCT CGGATTCCAT TGCCCAGCTA
      361 TCTGTCACTT CATCGAAAGG ACAGTAGAAA AGGAAGGTGG CTCCTACAAA TGCCATCATT
      421 GCGATAAAGG AAAGGCTATC ATTCAAGATC TCTCTGCCGA CAGTGGTCCC AAAGATGGAC
      481 CCCCACCCAC GAGGAGCATC GTGGAAAAAG AAGAGGTTCC AACCACGTCT ACAAAGCAAG
      541 TGGATTGATG TGACATCTCC ACTGACGTAA GGGATGACGC ACAATCCCAC TATCCTTCGC
      601 AAGACCCTTC CTCTATATAA GGAAGTTCAT TTCATTTGGA GAGGACACGC TCGAGTATAA
      661 GAGCTCATTT TTACAACAAT TACCAACAAC AACAAACAAC AAACAACATT ACAATTACAT
      721 TTACAATTAT CGATACAATG GGCAGCATTG ATTCAACAAA TGTAGCCATG TCCAATTCTC
      781 CAGTTGGAGA ATTTAAGCCA CTTGAAGCTG AGGAATTCCG AAAACAAGCC CATCGTATGG
      841 TAGATTTCAT AGCCGATTAT TACAAAAATG TGGAAACATA TCCGGTCCTT AGCGAAGTCG
      901 AACCTGGATA TCTCCGAAAA CGTATCCCCG AAACCGCTCC TTACCTCCCC GAACCACTTG
      961 ACGACATCAT GAAAGATATT CAGAAGGATA TTATCCCAGG AATGACAAAT TGGATGAGCC
     1021 CTAATTTTTA TGCATTTTTT CCTGCCACTG TTAGTTCAGC TGCCTTTTTA GGAGAAATGT
     1081 TGTCTACTGC CCTAAATTCA GTAGGCTTTA CTTGGGTTTC TTCACCAGCC GCCACCGAAT
     1141 TAGAAATGAT TGTTATGGAT TGGTTGGCTC AGATCCTTAA ACTCCCCAAA TCTTTCATGT
     1201 TTTCAGGTAC CGGTGGCGGC GTCATCCAAA ACACCACTAG CGAGTCCATT CTTTGTACAA
     1261 TCATTGCCGC CCGGGAAAGG GCCCTGGAGA AGCTCGGTCC CGATAGTATT GGAAAACTTG
     1321 TCTGTTACGG ATCCGATCAA ACCCATACCA TGTTCCCCAA AACTTGCAAA TTGGCGGGAA
     1381 TTTATCCGAA TAATATTAGG TTAATACCTA CGACCGTCGA AACGGATTTC GGCATCTCAC
     1441 CTCAAGTTCT ACGAAAAATG GTCGAGGATG ACGTGGCGGC CGGATATGTA CCGCTGTTCT
     1501 TATGCGCTAC CCTGGGTACC ACCTCGACCA CGGCTACCGA TCCTGTGGAC TCACTTTCTG
     1561 AAATCGCTAA CGAGTTTGGT ATTTGGATCC ACGTGGATGC TGCTTATGCG GGAAGCGCCT
     1621 GTATATGTCC CGAGTTTAGA CATTACTTGG ATGGAATCGA ACGAGTTGAC TCACTGAGTC
     1681 TGAGTCCACA CAAATGGCTA CTCGCTTACT TAGATTGCAC TTGCTTGTGG GTCAAGCAAC
     1741 CACATTTGTT ACTAAGGGCA CTCACTACGA ATCCTGAGTA TTTAAAAAAT AAACAGAGTG
     1801 ATTTAGACAA AGTTGTGGAC TTCAAAAATT GGCAAATCGC AACGGGACGA AAATTTCGGT
     1861 CGCTGAAACT TTGGCTCATT TTACGTAGCT ATGGAGTTGT TAATTTACAG AGTCATATTC
     1921 GTTCTGACGT CGCAATGGGC AAAATGTTCG AAGAATGGGT TAGATCAGAC TCCAGATTCG
     1981 AAATTGTGGT ACCGAGAAAC TTTTCTCTTG TTTGTTTTAG ATTAAAACCT GACGTTTCGA
     2041 GTTTACATGT AGAAGAAGTG AATAAGAAAC TTTTGGACAT GCTTAACTCG ACGGGACGAG
     2101 TTTATATGAC TCATACTATT GTGGGAGGCA TATACATGCT AAGACTGGCT GTTGGCTCGT
     2161 CACTAACTGA AGAACATCAT GTACGCCGTG TTTGGGATTT GATTCAAAAA TTAACCGATG
     2221 ATTTGCTCAA AGAAGCTTGA GCTTCTCTAG CTAGAGTCGA TCGACAAGCT CGAGTTTCTC
     2281 CATAATAATG TGTGAGTAGT TCCCAGATAA GGGAATTAGG GTTCCTATAG GGTTTCGCTC
     2341 ATGTGTTGAG CATATAAGAA ACCCTTAGTA TGTATTTGTA TTTGTAAAAT ACTTCTATCA
     2401 ATAAAATTTC TAATTCCTAA AACCAAAATC CAGTACTAAA ATCCAGATCG CTGCAATTGT
     2461 CTTCTGCACG AAGTGGTTTA AACTATCAGT GTTTGACAGG ATATATTGGC GGGTAAACCT
     2521 AAGAGAAAAG AGCGTTTATT AGAATAATCG GATATTTAAA AGGGCGTGAA AAGGTTTATC
     2581 CGTTCGTCCA TTTGTATGTG CATGCCAACC ACAGGGTTCC CCAGATCAGG CGCTGGCTGC
     2641 TGAACCCCCA GCCGGAACTG ACCCCACAAG GCCCTAGCGT TTGCAATGCA CCAGGTCATC
     2701 ATTGACCCAG GCGTGTTCCA CCAGGCCGCT GCCTCGCAAC TCTTCGCAGG CTTCGCCGAC
     2761 CTGCTCGCGC CACTTCTTCA CGCGGGTGGA ATCCGATCCG CACATGAGGC GGAAGGTTTC
     2821 CAGCTTGAGC GGGTACGGCT CCCGGTGCGA GCTGAAATAG TCGAACATCC GTCGGGCCGT
     2881 CGGCGACAGC TTGCGGTACT TCTCCCATAT GAATTTCGTG TAGTGGTCGC CAGCAAACAG
     2941 CACGACGATT TCCTCGTCGA TCAGGACCTG GCAACGGGAC GTTTTCTTGC CACGGTCCAG
     3001 GACGCGGAAG CGGTGCAGCA GCGACACCGA TTCCAGGTGC CCAACGCGGT CGGACGTGAA
     3061 GCCCATCGCC GTCGCCTGTA GGCGCGACAG GCATTCCTCG GCCTTCGTGT AATACCGGCC
     3121 ATTGATCGAC CAGCCCAGGT CCTGGCAAAG CTCGTAGAAC GTGAAGGTGA TCGGCTCGCC
     3181 GATAGGGGTG CGCTTCGCGT ACTCCAACAC CTGCTGCCAC ACCAGTTCGT CATCGTCGGC
     3241 CCGCAGCTCG ACGCCGGTGT AGGTGATCTT CACGTCCTTG TTGACGTGGA AAATGACCTT
     3301 GTTTTGCAGC GCCTCGCGCG GGATTTTCTT GTTGCGCGTG GTGAACAGGG CAGAGCGGGC
     3361 CGTGTCGTTT GGCATCGCTC GCATCGTGTC CGGCCACGGC GCAATATCGA ACAAGGAAAG
     3421 CTGCATTTCC TTGATCTGCT GCTTCGTGTG TTTCAGCAAC GCGGCCTGCT TGGCCTCGCT
     3481 GACCTGTTTT GCCAGGTCCT CGCCGGCGGT TTTTCGCTTC TTGGTCGTCA TAGTTCCTCG
     3541 CGTGTCGATG GTCATCGACT TCGCCAAACC TGCCGCCTCC TGTTCAAGAC GACGCGAACG
     3601 CTCCACGGCG GCCGATGGCG CGGGCAGGGC AGGGGGAGCC AGTTGCACGC TGTCGCGCTC
     3661 GATCTTGGCC GTAGCTTGCT GGACCATCGA GCCGACGGAC TGGAAGGTTT CGCGGGGCGC
     3721 ACGCATGACG GTGCGGCTTG CGATGGTTTC GGCATCCTCG GCGGAAAACC CCGCGTCGAT
     3781 CAGTTCTTGC CTGTATGCCT TCCGGTCAAA CGTCCGATTC ATTCACCCTC CTTGCGGGAT
     3841 TGCCCCGACT CACGCCGGGG CAATGTGCCC TTATTCCTGA TTTGACCCGC CTGGTGCCTT
     3901 GGTGTCCAGA TAATCCACCT TATCGGCAAT GAAGTCGGTC CCGTAGACCG TCTGGCCGTC
     3961 CTTCTCGTAC TTGGTATTCC GAATCTTGCC CTGCACGAAT ACCAGCGACC CCTTGCCCAA
     4021 ATACTTGCCG TGGGCCTCGG CCTGAGAGCC AAAACACTTG ATGCGGAAGA AGTCGGTGCG
     4081 CTCCTGCTTG TCGCCGGCAT CGTTGCGCCA CATCTAGGAT CTGCCAGGAA CCGTAAAAAG
     4141 GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA
     4201 CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT
     4261 GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC
     4321 TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG
     4381 GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC
     4441 TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA
     4501 CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG
     4561 TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG TATCTGCGCT
     4621 CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC
     4681 ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA
     4741 TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA
     4801 CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT
     4861 TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AAACTTGGTC TGACAGTTAC
     4921 CAATGCTTAA TCAGTGAGGC ACCTATCTCA GCGATCTGTC TATTTCGTTC ATCCATAGTT
     4981 GCCTGACTCC CCGTCGTGTA GATAACTACG ATACGGGAGG GCTTACCATC TGGCCCCAGT
     5041 GCTGCAATGA TACCGCGAGA ACCACGCTCA CCGGCTCCAG ATTTATCAGC AATAAACCAG
     5101 CCAGCCGGAA GGGCCGAGCG CAGAAGTGGT CCTGCAACTT TATCCGCCTC CATCCAGTCT
     5161 ATTAATTGTT GCCGGGAAGC TAGAGTAAGT AGTTCGCCAG TTAATAGTTT GCGCAACGTT
     5221 GTTGCCATTG CTACAGGCAT CGTGGTGTCA CGCTCGTCGT TTGGTATGGC TTCATTCAGC
     5281 TCCGGTTCCC AACGATCAAG GCGAGTTACA TGATCCCCCA TGTTGTGCAA AAAAGCGGTT
     5341 AGCTCCTTCG GTCCTCCGAT CGTTGTCAGA AGTAAGTTGG CCGCAGTGTT ATCACTCATG
     5401 GTTATGGCAG CACTGCATAA TTCTCTTACT GTCATGCCAT CCGTAAGATG CTTTTCTGTG
     5461 ACTGGTGAGT ACTCAACCAA GTCATTCTGA GAATAGTGTA TGCGGCGACC GAGTTGCTCT
     5521 TGCCCGGCGT CAATACGGGA TAATACCGCG CCACATAGCA GAACTTTAAA AGTGCTCATC
     5581 ATTGGAAAAC GTTCTTCGGG GCGAAAACTC TCAAGGATCT TACCGCTGTT GAGATCCAGT
     5641 TCGATGTAAC CCACTCGTGC ACCCAACTGA TCTTCAGCAT CTTTTACTTT CACCAGCGTT
     5701 TCTGGGTGAG CAAAAACAGG AAGGCAAAAT GCCGCAAAAA AGGGAATAAG GGCGACACGG
     5761 AAATGTTGAA TACTCATACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAAGGTTAT
     5821 TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG
     5881 CGCACGAATT GGCCAGCGCT GCCATTTTTG GGGTGAGGCC GTTCGCGGCC GAGGGGCGCA
     5941 GCCCCTGGGG GGATGGGAGG CCCGCGTTAG CGGGCCGGGA GGGTTCGAGA AGGGGGGGCA
     6001 CCCCCCTTCG GCGTGCGCGG TCACGCGCAC AGGGCGCAGC CCTGGTTAAA AACAAGGTTT
     6061 ATAAATATTG GTTTAAAAGC AGGTTAAAAG ACAGGTTAGC GGTGGCCGAA AAACGGGCGG
     6121 AAACCCTTGC AAATGCTGGA TTTTCTGCCT GTGGACAGCC CCTCAAATGT CAATAGGTGC
     6181 GCCCCTCATC TGTCAGCACT CTGCCCCTCA AGTGTCAAGG ATCGCGCCCC TCATCTGTCA
     6241 GTAGTCGCGC CCCTCAAGTG TCAATACCGC AGGGCACTTA TCCCCAGGCT TGTCCACATC
     6301 ATCTGTGGGA AACTCGCGTA AAATCAGGCG TTTTCGCCGA TTTGCGAGGC TGGCCAGCTC
     6361 CACGTCGCCG GCCGAAATCG AGCCTGCCCC TCATCTGTCA ACGCCGCGCC GGGTGAGTCG
     6421 GCCCCTCAAG TGTCAACGTC CGCCCCTCAT CTGTCAGTGA GGGCCAAGTT TTCCGCGAGG
     6481 TATCCACAAC GCCGGCGGCC GCGGTGTCTC GCACACGGCT TCGACGGCGT TTCTGGCGCG
     6541 TTTGCAGGGC CATAGACGGC CGCCAGCCCA GCGGCGAGGG CAACCAGCCC GGTGAGCGTC
//