LOCUS       pEPQD1CB0774_(P1_35Ssho 6777 bp ds-DNA     circular     13-OCT-2020
DEFINITION  .
FEATURES             Location/Qualifiers
     Misc._feature   22..169
                     /label="LB"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    complement(92..116)
                     /label="LB T-DNA repeat"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     Promoter        225..650
                     /label="CaMV 35S promoter short"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     None            228..553
                     /label="CaMV 35S promoter repeat unit"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     Golden_Gate_Par 651..917
                     /label="5U-TMV+SP-chloroplast"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     5'_UTR          655..737
                     /label="TMV omega"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     Transit_peptide 738..913
                     /label="chloroplast targeting signal"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     None            918..2414
                     /label="CrTDC tryptophan decarboxylase CDS, Catharanthus roseus, M25151 (domesticated, 1 BtgZI site removed)"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     Terminator      2422..2625
                     /label="35S ter"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    complement(2653..2778)
                     /label="RB"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    complement(2671..2695)
                     /label="RB T-DNA repeat"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     CDS             complement(2851..3999)
                     /label="Plasmid replication initiator protein TrfA, P07676 (TRFA_ECOLX)"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     rep_origin      complement(4335..4923)
                     /label="pUC origin of replication (derivative of pBR322/pMB1/ColE1)"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     primer_bind     complement(4380..4398)
                     /label="QMD062 primer"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     primer_bind     complement(4415..4434)
                     /label="pBR322ori-F"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     CDS             complement(5094..5954)
                     /label="beta-lactamase, ampicillin resistance (ampR)"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     promoter        complement(5955..6059)
                     /label="AmpR promoter"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     rep_origin      complement(6146..6777)
                     /label="RK2 (OriV) origin of replication (incP incompatibility group)"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
ORIGIN
        1 GCAAAGGAGA TCCTGATCTG ACTGATGGGC TGCCTGTATC GAGTGGTGAT TTTGTGCCGA
       61 GCTGCCGGTC GGGGAGCTGT TGGCTGGCTG GTGGCAGGAT ATATTGTGGT GTAAACAAAT
      121 TGACGCTTAG ACAACTTAAT AACACATTGC GGACGTTTTT AATGTACTGG GGTGGATGCA
      181 GTGGGCCCCA CTCTGTGAAG ACAATGCCGA ATTCGGATCC GGAGGTCAAC ATGGTGGAGC
      241 ACGACACTCT GGTCTACTCC AAAAATGTCA AAGATACAGT CTCAGAAGAT CAAAGGGCTA
      301 TTGAGACTTT TCAACAAAGG ATAATTTCGG GAAACCTCCT CGGATTCCAT TGCCCAGCTA
      361 TCTGTCACTT CATCGAAAGG ACAGTAGAAA AGGAAGGTGG CTCCTACAAA TGCCATCATT
      421 GCGATAAAGG AAAGGCTATC ATTCAAGATC TCTCTGCCGA CAGTGGTCCC AAAGATGGAC
      481 CCCCACCCAC GAGGAGCATC GTGGAAAAAG AAGAGGTTCC AACCACGTCT ACAAAGCAAG
      541 TGGATTGATG TGACATCTCC ACTGACGTAA GGGATGACGC ACAATCCCAC TATCCTTCGC
      601 AAGACCCTTC CTCTATATAA GGAAGTTCAT TTCATTTGGA GAGGACACGC TACTGTATAA
      661 GAGCTCATTT TTACAACAAT TACCAACAAC AACAAACAAC AAACAACATT ACAATTACAT
      721 TTACAATTAT CGATAACATG GCTTCTTCTA TGCTTTCTTC TGCTGCTGTT GTTGCTACTC
      781 GTGCTAGTGC TGCTCAAGCT AGTATGGTTG CTCCTTTTAC TGGACTTAAG TCTGCTGCTT
      841 CTTTTCCTGT TACTAGAAAG CAAAACAACC TTGATATTAC TTCTATTGCT AGTAACGGAG
      901 GAAGAGTTCG AGCAATGGGC AGCATTGATT CAACAAATGT AGCCATGTCC AATTCTCCAG
      961 TTGGAGAATT TAAGCCACTT GAAGCTGAGG AATTCCGAAA ACAAGCCCAT CGTATGGTAG
     1021 ATTTCATAGC CGATTATTAC AAAAATGTGG AAACATATCC GGTCCTTAGC GAAGTCGAAC
     1081 CTGGATATCT CCGAAAACGT ATCCCCGAAA CCGCTCCTTA CCTCCCCGAA CCACTTGACG
     1141 ACATCATGAA AGATATTCAG AAGGATATTA TCCCAGGAAT GACAAATTGG ATGAGCCCTA
     1201 ATTTTTATGC ATTTTTTCCT GCCACTGTTA GTTCAGCTGC CTTTTTAGGA GAAATGTTGT
     1261 CTACTGCCCT AAATTCAGTA GGCTTTACTT GGGTTTCTTC ACCAGCCGCC ACCGAATTAG
     1321 AAATGATTGT TATGGATTGG TTGGCTCAGA TCCTTAAACT CCCCAAATCT TTCATGTTTT
     1381 CAGGTACCGG TGGCGGCGTC ATCCAAAACA CCACTAGCGA GTCCATTCTT TGTACAATCA
     1441 TTGCCGCCCG GGAAAGGGCC CTGGAGAAGC TCGGTCCCGA TAGTATTGGA AAACTTGTCT
     1501 GTTACGGATC CGATCAAACC CATACCATGT TCCCCAAAAC TTGCAAATTG GCGGGAATTT
     1561 ATCCGAATAA TATTAGGTTA ATACCTACGA CCGTCGAAAC GGATTTCGGC ATCTCACCTC
     1621 AAGTTCTACG AAAAATGGTC GAGGATGACG TGGCGGCCGG ATATGTACCG CTGTTCTTAT
     1681 GCGCTACCCT GGGTACCACC TCGACCACGG CTACCGATCC TGTGGACTCA CTTTCTGAAA
     1741 TCGCTAACGA GTTTGGTATT TGGATCCACG TGGATGCTGC TTATGCGGGA AGCGCCTGTA
     1801 TATGTCCCGA GTTTAGACAT TACTTGGATG GAATCGAACG AGTTGACTCA CTGAGTCTGA
     1861 GTCCACACAA ATGGCTACTC GCTTACTTAG ATTGCACTTG CTTGTGGGTC AAGCAACCAC
     1921 ATTTGTTACT AAGGGCACTC ACTACGAATC CTGAGTATTT AAAAAATAAA CAGAGTGATT
     1981 TAGACAAAGT TGTGGACTTC AAAAATTGGC AAATCGCAAC GGGACGAAAA TTTCGGTCGC
     2041 TGAAACTTTG GCTCATTTTA CGTAGCTATG GAGTTGTTAA TTTACAGAGT CATATTCGTT
     2101 CTGACGTCGC AATGGGCAAA ATGTTCGAAG AATGGGTTAG ATCAGACTCC AGATTCGAAA
     2161 TTGTGGTACC GAGAAACTTT TCTCTTGTTT GTTTTAGATT AAAACCTGAC GTTTCGAGTT
     2221 TACATGTAGA AGAAGTGAAT AAGAAACTTT TGGACATGCT TAACTCGACG GGACGAGTTT
     2281 ATATGACTCA TACTATTGTG GGAGGCATAT ACATGCTAAG ACTGGCTGTT GGCTCGTCAC
     2341 TAACTGAAGA ACATCATGTA CGCCGTGTTT GGGATTTGAT TCAAAAATTA ACCGATGATT
     2401 TGCTCAAAGA AGCTTGAGCT TCTCTAGCTA GAGTCGATCG ACAAGCTCGA GTTTCTCCAT
     2461 AATAATGTGT GAGTAGTTCC CAGATAAGGG AATTAGGGTT CCTATAGGGT TTCGCTCATG
     2521 TGTTGAGCAT ATAAGAAACC CTTAGTATGT ATTTGTATTT GTAAAATACT TCTATCAATA
     2581 AAATTTCTAA TTCCTAAAAC CAAAATCCAG TACTAAAATC CAGATCGCTG CAATTGTCTT
     2641 CTGCACGAAG TGGTTTAAAC TATCAGTGTT TGACAGGATA TATTGGCGGG TAAACCTAAG
     2701 AGAAAAGAGC GTTTATTAGA ATAATCGGAT ATTTAAAAGG GCGTGAAAAG GTTTATCCGT
     2761 TCGTCCATTT GTATGTGCAT GCCAACCACA GGGTTCCCCA GATCAGGCGC TGGCTGCTGA
     2821 ACCCCCAGCC GGAACTGACC CCACAAGGCC CTAGCGTTTG CAATGCACCA GGTCATCATT
     2881 GACCCAGGCG TGTTCCACCA GGCCGCTGCC TCGCAACTCT TCGCAGGCTT CGCCGACCTG
     2941 CTCGCGCCAC TTCTTCACGC GGGTGGAATC CGATCCGCAC ATGAGGCGGA AGGTTTCCAG
     3001 CTTGAGCGGG TACGGCTCCC GGTGCGAGCT GAAATAGTCG AACATCCGTC GGGCCGTCGG
     3061 CGACAGCTTG CGGTACTTCT CCCATATGAA TTTCGTGTAG TGGTCGCCAG CAAACAGCAC
     3121 GACGATTTCC TCGTCGATCA GGACCTGGCA ACGGGACGTT TTCTTGCCAC GGTCCAGGAC
     3181 GCGGAAGCGG TGCAGCAGCG ACACCGATTC CAGGTGCCCA ACGCGGTCGG ACGTGAAGCC
     3241 CATCGCCGTC GCCTGTAGGC GCGACAGGCA TTCCTCGGCC TTCGTGTAAT ACCGGCCATT
     3301 GATCGACCAG CCCAGGTCCT GGCAAAGCTC GTAGAACGTG AAGGTGATCG GCTCGCCGAT
     3361 AGGGGTGCGC TTCGCGTACT CCAACACCTG CTGCCACACC AGTTCGTCAT CGTCGGCCCG
     3421 CAGCTCGACG CCGGTGTAGG TGATCTTCAC GTCCTTGTTG ACGTGGAAAA TGACCTTGTT
     3481 TTGCAGCGCC TCGCGCGGGA TTTTCTTGTT GCGCGTGGTG AACAGGGCAG AGCGGGCCGT
     3541 GTCGTTTGGC ATCGCTCGCA TCGTGTCCGG CCACGGCGCA ATATCGAACA AGGAAAGCTG
     3601 CATTTCCTTG ATCTGCTGCT TCGTGTGTTT CAGCAACGCG GCCTGCTTGG CCTCGCTGAC
     3661 CTGTTTTGCC AGGTCCTCGC CGGCGGTTTT TCGCTTCTTG GTCGTCATAG TTCCTCGCGT
     3721 GTCGATGGTC ATCGACTTCG CCAAACCTGC CGCCTCCTGT TCAAGACGAC GCGAACGCTC
     3781 CACGGCGGCC GATGGCGCGG GCAGGGCAGG GGGAGCCAGT TGCACGCTGT CGCGCTCGAT
     3841 CTTGGCCGTA GCTTGCTGGA CCATCGAGCC GACGGACTGG AAGGTTTCGC GGGGCGCACG
     3901 CATGACGGTG CGGCTTGCGA TGGTTTCGGC ATCCTCGGCG GAAAACCCCG CGTCGATCAG
     3961 TTCTTGCCTG TATGCCTTCC GGTCAAACGT CCGATTCATT CACCCTCCTT GCGGGATTGC
     4021 CCCGACTCAC GCCGGGGCAA TGTGCCCTTA TTCCTGATTT GACCCGCCTG GTGCCTTGGT
     4081 GTCCAGATAA TCCACCTTAT CGGCAATGAA GTCGGTCCCG TAGACCGTCT GGCCGTCCTT
     4141 CTCGTACTTG GTATTCCGAA TCTTGCCCTG CACGAATACC AGCGACCCCT TGCCCAAATA
     4201 CTTGCCGTGG GCCTCGGCCT GAGAGCCAAA ACACTTGATG CGGAAGAAGT CGGTGCGCTC
     4261 CTGCTTGTCG CCGGCATCGT TGCGCCACAT CTAGGATCTG CCAGGAACCG TAAAAAGGCC
     4321 GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC
     4381 TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA
     4441 AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT
     4501 CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG
     4561 TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC
     4621 GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG
     4681 GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC
     4741 TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG
     4801 CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC
     4861 GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT
     4921 CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT
     4981 TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA
     5041 AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAA CTTGGTCTGA CAGTTACCAA
     5101 TGCTTAATCA GTGAGGCACC TATCTCAGCG ATCTGTCTAT TTCGTTCATC CATAGTTGCC
     5161 TGACTCCCCG TCGTGTAGAT AACTACGATA CGGGAGGGCT TACCATCTGG CCCCAGTGCT
     5221 GCAATGATAC CGCGAGAACC ACGCTCACCG GCTCCAGATT TATCAGCAAT AAACCAGCCA
     5281 GCCGGAAGGG CCGAGCGCAG AAGTGGTCCT GCAACTTTAT CCGCCTCCAT CCAGTCTATT
     5341 AATTGTTGCC GGGAAGCTAG AGTAAGTAGT TCGCCAGTTA ATAGTTTGCG CAACGTTGTT
     5401 GCCATTGCTA CAGGCATCGT GGTGTCACGC TCGTCGTTTG GTATGGCTTC ATTCAGCTCC
     5461 GGTTCCCAAC GATCAAGGCG AGTTACATGA TCCCCCATGT TGTGCAAAAA AGCGGTTAGC
     5521 TCCTTCGGTC CTCCGATCGT TGTCAGAAGT AAGTTGGCCG CAGTGTTATC ACTCATGGTT
     5581 ATGGCAGCAC TGCATAATTC TCTTACTGTC ATGCCATCCG TAAGATGCTT TTCTGTGACT
     5641 GGTGAGTACT CAACCAAGTC ATTCTGAGAA TAGTGTATGC GGCGACCGAG TTGCTCTTGC
     5701 CCGGCGTCAA TACGGGATAA TACCGCGCCA CATAGCAGAA CTTTAAAAGT GCTCATCATT
     5761 GGAAAACGTT CTTCGGGGCG AAAACTCTCA AGGATCTTAC CGCTGTTGAG ATCCAGTTCG
     5821 ATGTAACCCA CTCGTGCACC CAACTGATCT TCAGCATCTT TTACTTTCAC CAGCGTTTCT
     5881 GGGTGAGCAA AAACAGGAAG GCAAAATGCC GCAAAAAAGG GAATAAGGGC GACACGGAAA
     5941 TGTTGAATAC TCATACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA AGGTTATTGT
     6001 CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC
     6061 ACGAATTGGC CAGCGCTGCC ATTTTTGGGG TGAGGCCGTT CGCGGCCGAG GGGCGCAGCC
     6121 CCTGGGGGGA TGGGAGGCCC GCGTTAGCGG GCCGGGAGGG TTCGAGAAGG GGGGGCACCC
     6181 CCCTTCGGCG TGCGCGGTCA CGCGCACAGG GCGCAGCCCT GGTTAAAAAC AAGGTTTATA
     6241 AATATTGGTT TAAAAGCAGG TTAAAAGACA GGTTAGCGGT GGCCGAAAAA CGGGCGGAAA
     6301 CCCTTGCAAA TGCTGGATTT TCTGCCTGTG GACAGCCCCT CAAATGTCAA TAGGTGCGCC
     6361 CCTCATCTGT CAGCACTCTG CCCCTCAAGT GTCAAGGATC GCGCCCCTCA TCTGTCAGTA
     6421 GTCGCGCCCC TCAAGTGTCA ATACCGCAGG GCACTTATCC CCAGGCTTGT CCACATCATC
     6481 TGTGGGAAAC TCGCGTAAAA TCAGGCGTTT TCGCCGATTT GCGAGGCTGG CCAGCTCCAC
     6541 GTCGCCGGCC GAAATCGAGC CTGCCCCTCA TCTGTCAACG CCGCGCCGGG TGAGTCGGCC
     6601 CCTCAAGTGT CAACGTCCGC CCCTCATCTG TCAGTGAGGG CCAAGTTTTC CGCGAGGTAT
     6661 CCACAACGCC GGCGGCCGCG GTGTCTCGCA CACGGCTTCG ACGGCGTTTC TGGCGCGTTT
     6721 GCAGGGCCAT AGACGGCCGC CAGCCCAGCG GCGAGGGCAA CCAGCCCGGT GAGCGTC
//