LOCUS       pEPQD1CB0775_(P2_35Ssho 6156 bp ds-DNA     circular     13-OCT-2020
DEFINITION  .
FEATURES             Location/Qualifiers
     Misc._feature   22..169
                     /label="LB"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    complement(92..116)
                     /label="LB T-DNA repeat"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     Promoter        225..650
                     /label="CaMV 35S promoter short"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     None            228..553
                     /label="CaMV 35S promoter repeat unit"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     None            655..735
                     /label="TMV omega (two 3' bp removed)"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     None            741..1793
                     /label="CrSTR2 strictosidine synthase, Catharanthus roseus, X61932 (domesticated, 1 BbsI site removed, L19-S32 recoded to avoid repeats)"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     Terminator      1801..2004
                     /label="35S ter"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    complement(2032..2157)
                     /label="RB"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    complement(2050..2074)
                     /label="RB T-DNA repeat"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     CDS             complement(2230..3378)
                     /label="Plasmid replication initiator protein TrfA, P07676 (TRFA_ECOLX)"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     rep_origin      complement(3714..4302)
                     /label="pBR322 origin of replication (derivative of pMB1/ColE1)"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     primer_bind     complement(3759..3777)
                     /label="QMD062 primer"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     primer_bind     complement(3794..3813)
                     /label="pBR322ori-F"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     CDS             complement(4473..5333)
                     /label="beta-lactamase, ampicillin resistance (ampR)"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     rep_origin      complement(5525..6156)
                     /label="RK2 (OriV) origin of replication (incP incompatibility group)"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
ORIGIN
        1 GCAAAGGAGA TCCTGATCTG ACTGATGGGC TGCCTGTATC GAGTGGTGAT TTTGTGCCGA
       61 GCTGCCGGTC GGGGAGCTGT TGGCTGGCTG GTGGCAGGAT ATATTGTGGT GTAAACAAAT
      121 TGACGCTTAG ACAACTTAAT AACACATTGC GGACGTTTTT AATGTACTGG GGTGGATGCA
      181 GTGGGCCCCA CTCTGTGAAG ACAAGCAAGA ATTCAAGCTT GGAGGTCAAC ATGGTGGAGC
      241 ACGACACTCT GGTCTACTCC AAAAATGTCA AAGATACAGT CTCAGAAGAT CAAAGGGCTA
      301 TTGAGACTTT TCAACAAAGG ATAATTTCGG GAAACCTCCT CGGATTCCAT TGCCCAGCTA
      361 TCTGTCACTT CATCGAAAGG ACAGTAGAAA AGGAAGGTGG CTCCTACAAA TGCCATCATT
      421 GCGATAAAGG AAAGGCTATC ATTCAAGATC TCTCTGCCGA CAGTGGTCCC AAAGATGGAC
      481 CCCCACCCAC GAGGAGCATC GTGGAAAAAG AAGAGGTTCC AACCACGTCT ACAAAGCAAG
      541 TGGATTGATG TGACATCTCC ACTGACGTAA GGGATGACGC ACAATCCCAC TATCCTTCGC
      601 AAGACCCTTC CTCTATATAA GGAAGTTCAT TTCATTTGGA GAGGACACGC TCGAGTATAA
      661 GAGCTCATTT TTACAACAAT TACCAACAAC AACAAACAAC AAACAACATT ACAATTACAT
      721 TTACAATTAT CGATACAATG GCAAACTTTT CTGAATCTAA ATCCATGATG GCAGTTTTCT
      781 TCATGTTTTT CCTGCTTCTT TTGTCATCTT CTAGTTCTTC ATCTTCAAGT TCACCAATTT
      841 TGAAAAAGAT TTTTATTGAA AGCCCTTCCT ATGCTCCGAA TGCCTTCACC TTCGATTCAA
      901 CTGATAAAGG GTTCTACACT TCCGTCCAAG ATGGCCGAGT TATCAAATAT GAAGGGCCAA
      961 ATTCAGGCTT CACTGACTTC GCCTACGCAT CTCCCTTCTG GAACAAAGCT TTTTGTGAGA
     1021 ACAGCACCGA TCCAGAGAAA AGACCATTGT GTGGGAGGAC ATATGATATT TCCTATGACT
     1081 ATAAGAACAG CCAAATGTAC ATTGTTGATG GCCATTACCA TCTTTGTGTG GTTGGAAAAG
     1141 AAGGTGGGTA TGCCACACAA CTAGCCACAA GTGTGCAAGG AGTGCCATTC AAATGGCTCT
     1201 ATGCAGTAAC TGTTGATCAG AGAACAGGGA TTGTTTATTT CACTGATGTT AGCTCCATAC
     1261 ATGATGACAG TCCCGAAGGT GTGGAAGAAA TCATGAATAC AAGTGATAGA ACAGGGAGAT
     1321 TAATGAAGTA TGATCCTTCA ACAAAAGAAA CCACCTTATT ATTGAAAGAG CTACATGTTC
     1381 CCGGCGGTGC AGAAATCAGC GCAGATGGTT CCTTTGTTGT AGTAGCAGAA TTTTTAAGCA
     1441 ATCGGATAGT GAAGTATTGG CTAGAAGGGC CAAAGAAAGG CAGTGCAGAG TTCTTAGTTA
     1501 CAATCCCAAA TCCAGGAAAT ATAAAGAGGA ATTCTGATGG CCATTTTTGG GTGTCATCTA
     1561 GTGAAGAATT AGATGGAGGT CAACATGGAA GAGTTGTTTC AAGAGGAATT AAGTTTGATG
     1621 GATTTGGGAA TATTCTTCAA GTTATACCAC TTCCACCACC ATATGAAGGT GAACATTTTG
     1681 AACAGATTCA AGAGCACGAT GGTTTGTTAT ACATTGGAAG TCTCTTCCAT AGCTCTGTGG
     1741 GTATATTAGT GTATGATGAT CATGATAACA AGGGAAATTC TTATGTTTCT AGCTGAGCTT
     1801 CTCTAGCTAG AGTCGATCGA CAAGCTCGAG TTTCTCCATA ATAATGTGTG AGTAGTTCCC
     1861 AGATAAGGGA ATTAGGGTTC CTATAGGGTT TCGCTCATGT GTTGAGCATA TAAGAAACCC
     1921 TTAGTATGTA TTTGTATTTG TAAAATACTT CTATCAATAA AATTTCTAAT TCCTAAAACC
     1981 AAAATCCAGT ACTAAAATCC AGATCGCTAC TATTGTCTTC TGCACGAAGT GGTTTAAACT
     2041 ATCAGTGTTT GACAGGATAT ATTGGCGGGT AAACCTAAGA GAAAAGAGCG TTTATTAGAA
     2101 TAATCGGATA TTTAAAAGGG CGTGAAAAGG TTTATCCGTT CGTCCATTTG TATGTGCATG
     2161 CCAACCACAG GGTTCCCCAG ATCAGGCGCT GGCTGCTGAA CCCCCAGCCG GAACTGACCC
     2221 CACAAGGCCC TAGCGTTTGC AATGCACCAG GTCATCATTG ACCCAGGCGT GTTCCACCAG
     2281 GCCGCTGCCT CGCAACTCTT CGCAGGCTTC GCCGACCTGC TCGCGCCACT TCTTCACGCG
     2341 GGTGGAATCC GATCCGCACA TGAGGCGGAA GGTTTCCAGC TTGAGCGGGT ACGGCTCCCG
     2401 GTGCGAGCTG AAATAGTCGA ACATCCGTCG GGCCGTCGGC GACAGCTTGC GGTACTTCTC
     2461 CCATATGAAT TTCGTGTAGT GGTCGCCAGC AAACAGCACG ACGATTTCCT CGTCGATCAG
     2521 GACCTGGCAA CGGGACGTTT TCTTGCCACG GTCCAGGACG CGGAAGCGGT GCAGCAGCGA
     2581 CACCGATTCC AGGTGCCCAA CGCGGTCGGA CGTGAAGCCC ATCGCCGTCG CCTGTAGGCG
     2641 CGACAGGCAT TCCTCGGCCT TCGTGTAATA CCGGCCATTG ATCGACCAGC CCAGGTCCTG
     2701 GCAAAGCTCG TAGAACGTGA AGGTGATCGG CTCGCCGATA GGGGTGCGCT TCGCGTACTC
     2761 CAACACCTGC TGCCACACCA GTTCGTCATC GTCGGCCCGC AGCTCGACGC CGGTGTAGGT
     2821 GATCTTCACG TCCTTGTTGA CGTGGAAAAT GACCTTGTTT TGCAGCGCCT CGCGCGGGAT
     2881 TTTCTTGTTG CGCGTGGTGA ACAGGGCAGA GCGGGCCGTG TCGTTTGGCA TCGCTCGCAT
     2941 CGTGTCCGGC CACGGCGCAA TATCGAACAA GGAAAGCTGC ATTTCCTTGA TCTGCTGCTT
     3001 CGTGTGTTTC AGCAACGCGG CCTGCTTGGC CTCGCTGACC TGTTTTGCCA GGTCCTCGCC
     3061 GGCGGTTTTT CGCTTCTTGG TCGTCATAGT TCCTCGCGTG TCGATGGTCA TCGACTTCGC
     3121 CAAACCTGCC GCCTCCTGTT CAAGACGACG CGAACGCTCC ACGGCGGCCG ATGGCGCGGG
     3181 CAGGGCAGGG GGAGCCAGTT GCACGCTGTC GCGCTCGATC TTGGCCGTAG CTTGCTGGAC
     3241 CATCGAGCCG ACGGACTGGA AGGTTTCGCG GGGCGCACGC ATGACGGTGC GGCTTGCGAT
     3301 GGTTTCGGCA TCCTCGGCGG AAAACCCCGC GTCGATCAGT TCTTGCCTGT ATGCCTTCCG
     3361 GTCAAACGTC CGATTCATTC ACCCTCCTTG CGGGATTGCC CCGACTCACG CCGGGGCAAT
     3421 GTGCCCTTAT TCCTGATTTG ACCCGCCTGG TGCCTTGGTG TCCAGATAAT CCACCTTATC
     3481 GGCAATGAAG TCGGTCCCGT AGACCGTCTG GCCGTCCTTC TCGTACTTGG TATTCCGAAT
     3541 CTTGCCCTGC ACGAATACCA GCGACCCCTT GCCCAAATAC TTGCCGTGGG CCTCGGCCTG
     3601 AGAGCCAAAA CACTTGATGC GGAAGAAGTC GGTGCGCTCC TGCTTGTCGC CGGCATCGTT
     3661 GCGCCACATC TAGGATCTGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT
     3721 AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC
     3781 CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT
     3841 GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG
     3901 CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG
     3961 GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT
     4021 CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG
     4081 ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC
     4141 GGCTACACTA GAAGGACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA
     4201 AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT
     4261 GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT
     4321 TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA
     4381 TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC
     4441 TAAAGTATAT ATGAGTAAAC TTGGTCTGAC AGTTACCAAT GCTTAATCAG TGAGGCACCT
     4501 ATCTCAGCGA TCTGTCTATT TCGTTCATCC ATAGTTGCCT GACTCCCCGT CGTGTAGATA
     4561 ACTACGATAC GGGAGGGCTT ACCATCTGGC CCCAGTGCTG CAATGATACC GCGAGAACCA
     4621 CGCTCACCGG CTCCAGATTT ATCAGCAATA AACCAGCCAG CCGGAAGGGC CGAGCGCAGA
     4681 AGTGGTCCTG CAACTTTATC CGCCTCCATC CAGTCTATTA ATTGTTGCCG GGAAGCTAGA
     4741 GTAAGTAGTT CGCCAGTTAA TAGTTTGCGC AACGTTGTTG CCATTGCTAC AGGCATCGTG
     4801 GTGTCACGCT CGTCGTTTGG TATGGCTTCA TTCAGCTCCG GTTCCCAACG ATCAAGGCGA
     4861 GTTACATGAT CCCCCATGTT GTGCAAAAAA GCGGTTAGCT CCTTCGGTCC TCCGATCGTT
     4921 GTCAGAAGTA AGTTGGCCGC AGTGTTATCA CTCATGGTTA TGGCAGCACT GCATAATTCT
     4981 CTTACTGTCA TGCCATCCGT AAGATGCTTT TCTGTGACTG GTGAGTACTC AACCAAGTCA
     5041 TTCTGAGAAT AGTGTATGCG GCGACCGAGT TGCTCTTGCC CGGCGTCAAT ACGGGATAAT
     5101 ACCGCGCCAC ATAGCAGAAC TTTAAAAGTG CTCATCATTG GAAAACGTTC TTCGGGGCGA
     5161 AAACTCTCAA GGATCTTACC GCTGTTGAGA TCCAGTTCGA TGTAACCCAC TCGTGCACCC
     5221 AACTGATCTT CAGCATCTTT TACTTTCACC AGCGTTTCTG GGTGAGCAAA AACAGGAAGG
     5281 CAAAATGCCG CAAAAAAGGG AATAAGGGCG ACACGGAAAT GTTGAATACT CATACTCTTC
     5341 CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT
     5401 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CGAATTGGCC AGCGCTGCCA
     5461 TTTTTGGGGT GAGGCCGTTC GCGGCCGAGG GGCGCAGCCC CTGGGGGGAT GGGAGGCCCG
     5521 CGTTAGCGGG CCGGGAGGGT TCGAGAAGGG GGGGCACCCC CCTTCGGCGT GCGCGGTCAC
     5581 GCGCACAGGG CGCAGCCCTG GTTAAAAACA AGGTTTATAA ATATTGGTTT AAAAGCAGGT
     5641 TAAAAGACAG GTTAGCGGTG GCCGAAAAAC GGGCGGAAAC CCTTGCAAAT GCTGGATTTT
     5701 CTGCCTGTGG ACAGCCCCTC AAATGTCAAT AGGTGCGCCC CTCATCTGTC AGCACTCTGC
     5761 CCCTCAAGTG TCAAGGATCG CGCCCCTCAT CTGTCAGTAG TCGCGCCCCT CAAGTGTCAA
     5821 TACCGCAGGG CACTTATCCC CAGGCTTGTC CACATCATCT GTGGGAAACT CGCGTAAAAT
     5881 CAGGCGTTTT CGCCGATTTG CGAGGCTGGC CAGCTCCACG TCGCCGGCCG AAATCGAGCC
     5941 TGCCCCTCAT CTGTCAACGC CGCGCCGGGT GAGTCGGCCC CTCAAGTGTC AACGTCCGCC
     6001 CCTCATCTGT CAGTGAGGGC CAAGTTTTCC GCGAGGTATC CACAACGCCG GCGGCCGCGG
     6061 TGTCTCGCAC ACGGCTTCGA CGGCGTTTCT GGCGCGTTTG CAGGGCCATA GACGGCCGCC
     6121 AGCCCAGCGG CGAGGGCAAC CAGCCCGGTG AGCGTC
//