LOCUS       pEPQD1CB0911_(P5_35Ssho 6222 bp ds-DNA     circular     10-JUN-2021
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    complement(23..173)
                     /label="LB"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    complement(92..116)
                     /label="LB T-DNA repeat"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     Promoter        225..650
                     /label="CaMV 35S promoter short"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     5'_UTR          667..728
                     /label="TMV Omega"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    741..1859
                     /label="MsLAMT loganic acid O-methyltransferase CDS, from Mitragyna speciosa (kratom) - domesticated (1 BbsI site removed), note Q262 removed compared to reference sequence from O'Connor lab (Yindi)"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    complement(1429..1434)
                     /label="domesticated BbsI"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     Terminator      1867..2070
                     /label="CaMV 35S terminator, pICH41414"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    complement(2081..2086)
                     /label="BpiI"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    complement(2098..2223)
                     /label="RB"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    complement(2116..2140)
                     /label="RB T-DNA repeat"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     conflict        2205..2223
                     /label="partial overdrive"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     Origin          complement(2253..3734)
                     /label="RK2"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     CDS             complement(2296..3444)
                     /label="Plasmid replication initiator protein TrfA, P07676 (TRFA_ECOLX)"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     primer_bind     complement(3860..3879)
                     /label="pBR322ori-F"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     CDS             complement(4539..5399)
                     /label="AmpR"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     rep_origin      complement(5591..6222)
                     /label="oriV"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
ORIGIN
        1 GCAAAGGAGA TCCTGATCTG ACTGATGGGC TGCCTGTATC GAGTGGTGAT TTTGTGCCGA
       61 GCTGCCGGTC GGGGAGCTGT TGGCTGGCTG GTGGCAGGAT ATATTGTGGT GTAAACAAAT
      121 TGACGCTTAG ACAACTTAAT AACACATTGC GGACGTTTTT AATGTACTGG GGTGGATGCA
      181 GTGGGCCCCA CTCTGTGAAG ACAACAGAGA ATTCGCATGC GGAGGTCAAC ATGGTGGAGC
      241 ACGACACTCT GGTCTACTCC AAAAATGTCA AAGATACAGT CTCAGAAGAT CAAAGGGCTA
      301 TTGAGACTTT TCAACAAAGG ATAATTTCGG GAAACCTCCT CGGATTCCAT TGCCCAGCTA
      361 TCTGTCACTT CATCGAAAGG ACAGTAGAAA AGGAAGGTGG CTCCTACAAA TGCCATCATT
      421 GCGATAAAGG AAAGGCTATC ATTCAAGATC TCTCTGCCGA CAGTGGTCCC AAAGATGGAC
      481 CCCCACCCAC GAGGAGCATC GTGGAAAAAG AAGAGGTTCC AACCACGTCT ACAAAGCAAG
      541 TGGATTGATG TGACATCTCC ACTGACGTAA GGGATGACGC ACAATCCCAC TATCCTTCGC
      601 AAGACCCTTC CTCTATATAA GGAAGTTCAT TTCATTTGGA GAGGACACGC TCGAGTATAA
      661 GAGCTCATTT TTACAACAAT TACCAACAAC AACAAACAAC AAACAACATT ACAATTACAT
      721 TTACAATTAT CGATACAATG GCCCCAACAC TGGACACCAT CCCAACTGTA GATGTCAAGG
      781 AAATGCCTGA AGAAGCGCAT CCTATGAAAG GCGGTGATGA CTTCAATAGC TATTCTCAGA
      841 ATTCTTGCTA TCAGAGGGGA GTTATAGATG CTGCAAAAGT AGTTATCGTT GAAGCAGTAA
      901 ATGAAAAGCT GGATCTAAAG AATAATGCTA ATTTTGATCC ATCTAAGCCT TTCCACATTG
      961 CTGATTTTGG GTGCTCAACC GGCCCTAACA CATATTTTGC CGTGCAAAAT ATTGTAGAAG
     1021 CCGTGGACCA AAAACACAAA TCCCTTCACG AAAAAATCCA GCCAGAATTC CATGTTTTTT
     1081 TCAATGATCA TGTCAACAAT GATTTCAACA TTCTCTTCAG ATCTCTCCCA CAGGACAGGG
     1141 ATTATTTTGC TGCTGGGGTC CCTGGATCAT TTTACACAAG GGTTTTTCCC AAGGCCAGTG
     1201 TACATTTTGC TCATTGCTCG TATGCACTTC ACTGGCTGTC CAAGGTTCCT AAGGAAATTC
     1261 AGGACAAGAA CTCTCCTGCC TTTAACAGAG GGAGAATTCA TTACACTGGG ACTGAAAAAC
     1321 ATGTCGTGAA AGCTTACTTT TCTCAATTCC AAAAAGACAT GGGCTCATTT CTGAAAGCTA
     1381 GAGCTCAAGA AATTGTGGGA GGAGGGTTGG TGGTGATTCA AATTCCCGGA CTTCCAAGTG
     1441 GTGAGGTCCT CTTCTCTAGG ACCGGTGCTG GCATGCTTCA TGCTCTTTTG GGATCTTCCT
     1501 TGATGGAACT CGTAAATTTG GGAATAATAA CCGAAGAAAG TGTGGACTCC TTCAACTTGC
     1561 CTCAATACCA TCCATCAATT GAGGAATTGG AAATGGTGAT AGAGATGAAC AATAGTTTTA
     1621 CCATTGAAAA AGTTGGAGCA TTGAATCACC CCATGAAGAA CTTACCATTT GACGTTCAAA
     1681 TGACTTCTCT GCAAATAAGA GCTGTCATGG AGGGGATTCT GTCTGATCAT TTTGGAGGAA
     1741 AAATCTTGGA TCAATTGTTC GAAATTTACA CCACGAAACT GCAAGAAAAT TACAACGTTT
     1801 TCGACAAGGA AATCCGAAGG GATGCTGATT TGTTCCTTGT TCTAAAACGT AAGAGCAATt
     1861 gaGCTTCTCT AGCTAGAGTC GATCGACAAG CTCGAGTTTC TCCATAATAA TGTGTGAGTA
     1921 GTTCCCAGAT AAGGGAATTA GGGTTCCTAT AGGGTTTCGC TCATGTGTTG AGCATATAAG
     1981 AAACCCTTAG TATGTATTTG TATTTGTAAA ATACTTCTAT CAATAAAATT TCTAATTCCT
     2041 AAAACCAAAA TCCAGTACTA AAATCCAGAT CGCTTGTGTT GTCTTCTGCA CGAAGTGGTT
     2101 TAAACTATCA GTGTTTGACA GGATATATTG GCGGGTAAAC CTAAGAGAAA AGAGCGTTTA
     2161 TTAGAATAAT CGGATATTTA AAAGGGCGTG AAAAGGTTTA TCCGTTCGTC CATTTGTATG
     2221 TGCATGCCAA CCACAGGGTT CCCCAGATCA GGCGCTGGCT GCTGAACCCC CAGCCGGAAC
     2281 TGACCCCACA AGGCCCTAGC GTTTGCAATG CACCAGGTCA TCATTGACCC AGGCGTGTTC
     2341 CACCAGGCCG CTGCCTCGCA ACTCTTCGCA GGCTTCGCCG ACCTGCTCGC GCCACTTCTT
     2401 CACGCGGGTG GAATCCGATC CGCACATGAG GCGGAAGGTT TCCAGCTTGA GCGGGTACGG
     2461 CTCCCGGTGC GAGCTGAAAT AGTCGAACAT CCGTCGGGCC GTCGGCGACA GCTTGCGGTA
     2521 CTTCTCCCAT ATGAATTTCG TGTAGTGGTC GCCAGCAAAC AGCACGACGA TTTCCTCGTC
     2581 GATCAGGACC TGGCAACGGG ACGTTTTCTT GCCACGGTCC AGGACGCGGA AGCGGTGCAG
     2641 CAGCGACACC GATTCCAGGT GCCCAACGCG GTCGGACGTG AAGCCCATCG CCGTCGCCTG
     2701 TAGGCGCGAC AGGCATTCCT CGGCCTTCGT GTAATACCGG CCATTGATCG ACCAGCCCAG
     2761 GTCCTGGCAA AGCTCGTAGA ACGTGAAGGT GATCGGCTCG CCGATAGGGG TGCGCTTCGC
     2821 GTACTCCAAC ACCTGCTGCC ACACCAGTTC GTCATCGTCG GCCCGCAGCT CGACGCCGGT
     2881 GTAGGTGATC TTCACGTCCT TGTTGACGTG GAAAATGACC TTGTTTTGCA GCGCCTCGCG
     2941 CGGGATTTTC TTGTTGCGCG TGGTGAACAG GGCAGAGCGG GCCGTGTCGT TTGGCATCGC
     3001 TCGCATCGTG TCCGGCCACG GCGCAATATC GAACAAGGAA AGCTGCATTT CCTTGATCTG
     3061 CTGCTTCGTG TGTTTCAGCA ACGCGGCCTG CTTGGCCTCG CTGACCTGTT TTGCCAGGTC
     3121 CTCGCCGGCG GTTTTTCGCT TCTTGGTCGT CATAGTTCCT CGCGTGTCGA TGGTCATCGA
     3181 CTTCGCCAAA CCTGCCGCCT CCTGTTCAAG ACGACGCGAA CGCTCCACGG CGGCCGATGG
     3241 CGCGGGCAGG GCAGGGGGAG CCAGTTGCAC GCTGTCGCGC TCGATCTTGG CCGTAGCTTG
     3301 CTGGACCATC GAGCCGACGG ACTGGAAGGT TTCGCGGGGC GCACGCATGA CGGTGCGGCT
     3361 TGCGATGGTT TCGGCATCCT CGGCGGAAAA CCCCGCGTCG ATCAGTTCTT GCCTGTATGC
     3421 CTTCCGGTCA AACGTCCGAT TCATTCACCC TCCTTGCGGG ATTGCCCCGA CTCACGCCGG
     3481 GGCAATGTGC CCTTATTCCT GATTTGACCC GCCTGGTGCC TTGGTGTCCA GATAATCCAC
     3541 CTTATCGGCA ATGAAGTCGG TCCCGTAGAC CGTCTGGCCG TCCTTCTCGT ACTTGGTATT
     3601 CCGAATCTTG CCCTGCACGA ATACCAGCGA CCCCTTGCCC AAATACTTGC CGTGGGCCTC
     3661 GGCCTGAGAG CCAAAACACT TGATGCGGAA GAAGTCGGTG CGCTCCTGCT TGTCGCCGGC
     3721 ATCGTTGCGC CACATCTAGG ATCTGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT
     3781 TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG
     3841 CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC
     3901 TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC
     3961 GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC
     4021 AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC
     4081 TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT
     4141 AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT
     4201 AACTACGGCT ACACTAGAAG GACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC
     4261 TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT
     4321 TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG
     4381 ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC
     4441 ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA
     4501 TCAATCTAAA GTATATATGA GTAAACTTGG TCTGACAGTT ACCAATGCTT AATCAGTGAG
     4561 GCACCTATCT CAGCGATCTG TCTATTTCGT TCATCCATAG TTGCCTGACT CCCCGTCGTG
     4621 TAGATAACTA CGATACGGGA GGGCTTACCA TCTGGCCCCA GTGCTGCAAT GATACCGCGA
     4681 GAACCACGCT CACCGGCTCC AGATTTATCA GCAATAAACC AGCCAGCCGG AAGGGCCGAG
     4741 CGCAGAAGTG GTCCTGCAAC TTTATCCGCC TCCATCCAGT CTATTAATTG TTGCCGGGAA
     4801 GCTAGAGTAA GTAGTTCGCC AGTTAATAGT TTGCGCAACG TTGTTGCCAT TGCTACAGGC
     4861 ATCGTGGTGT CACGCTCGTC GTTTGGTATG GCTTCATTCA GCTCCGGTTC CCAACGATCA
     4921 AGGCGAGTTA CATGATCCCC CATGTTGTGC AAAAAAGCGG TTAGCTCCTT CGGTCCTCCG
     4981 ATCGTTGTCA GAAGTAAGTT GGCCGCAGTG TTATCACTCA TGGTTATGGC AGCACTGCAT
     5041 AATTCTCTTA CTGTCATGCC ATCCGTAAGA TGCTTTTCTG TGACTGGTGA GTACTCAACC
     5101 AAGTCATTCT GAGAATAGTG TATGCGGCGA CCGAGTTGCT CTTGCCCGGC GTCAATACGG
     5161 GATAATACCG CGCCACATAG CAGAACTTTA AAAGTGCTCA TCATTGGAAA ACGTTCTTCG
     5221 GGGCGAAAAC TCTCAAGGAT CTTACCGCTG TTGAGATCCA GTTCGATGTA ACCCACTCGT
     5281 GCACCCAACT GATCTTCAGC ATCTTTTACT TTCACCAGCG TTTCTGGGTG AGCAAAAACA
     5341 GGAAGGCAAA ATGCCGCAAA AAAGGGAATA AGGGCGACAC GGAAATGTTG AATACTCATA
     5401 CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC
     5461 ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACGAA TTGGCCAGCG
     5521 CTGCCATTTT TGGGGTGAGG CCGTTCGCGG CCGAGGGGCG CAGCCCCTGG GGGGATGGGA
     5581 GGCCCGCGTT AGCGGGCCGG GAGGGTTCGA GAAGGGGGGG CACCCCCCTT CGGCGTGCGC
     5641 GGTCACGCGC ACAGGGCGCA GCCCTGGTTA AAAACAAGGT TTATAAATAT TGGTTTAAAA
     5701 GCAGGTTAAA AGACAGGTTA GCGGTGGCCG AAAAACGGGC GGAAACCCTT GCAAATGCTG
     5761 GATTTTCTGC CTGTGGACAG CCCCTCAAAT GTCAATAGGT GCGCCCCTCA TCTGTCAGCA
     5821 CTCTGCCCCT CAAGTGTCAA GGATCGCGCC CCTCATCTGT CAGTAGTCGC GCCCCTCAAG
     5881 TGTCAATACC GCAGGGCACT TATCCCCAGG CTTGTCCACA TCATCTGTGG GAAACTCGCG
     5941 TAAAATCAGG CGTTTTCGCC GATTTGCGAG GCTGGCCAGC TCCACGTCGC CGGCCGAAAT
     6001 CGAGCCTGCC CCTCATCTGT CAACGCCGCG CCGGGTGAGT CGGCCCCTCA AGTGTCAACG
     6061 TCCGCCCCTC ATCTGTCAGT GAGGGCCAAG TTTTCCGCGA GGTATCCACA ACGCCGGCGG
     6121 CCGCGGTGTC TCGCACACGG CTTCGACGGC GTTTCTGGCG CGTTTGCAGG GCCATAGACG
     6181 GCCGCCAGCC CAGCGGCGAG GGCAACCAGC CCGGTGAGCG TC
//