LOCUS       pEPQD1CB0905_(P1_35S_lo 8131 bp ds-DNA     circular     17-FEB-2021
DEFINITION  .
FEATURES             Location/Qualifiers
     CDS             complement(265..1125)
                     /label="AmpR"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     promoter        complement(1126..1230)
                     /label="AmpR promoter"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     rep_origin      complement(1317..1948)
                     /label="oriV"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     Misc._feature   1970..2117
                     /label="LB"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    complement(2040..2064)
                     /label="LB T-DNA repeat"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     Promoter        2173..3510
                     /label="35s (Cauliflower Mosaic Virus)"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     5'_UTR          3527..3588
                     /label="Omega (Tobacco Mosaic Virus)"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     CDS             3602..3850
                     /label="Gal4AD"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     CDS             3851..3879
                     /label="Linker"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    3884..5695
                     /label="phiC31 Integrase (UniProt Q9T221), N benthamiana codon optimized, BpiI domesticated  "
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    3884..5716
                     /label="phiC31 integrase plus NLS"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    5696..5716
                     /label="SV40 NLS (PKKKRKV)"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     Terminator      5724..5927
                     /label="CaMV 35S terminator, pICH41414"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     Misc._feature   complement(5959..6086)
                     /label="RB short"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    complement(5973..5997)
                     /label="RB T-DNA repeat"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     conflict        6062..6080
                     /label="partial overdrive"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     Origin          complement(6110..7591)
                     /label="RK2"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     CDS             complement(6153..7301)
                     /label="Plasmid replication initiator protein TrfA, P07676 (TRFA_ECOLX)"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    complement(join(7622..8131,1..105))
                     /label="replicon of pMB1"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     rep_origin      complement(join(7622..8131,1..110))
                     /label="pBR322\(from\pUC18)"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     primer_bind     complement(7717..7736)
                     /label="pBR322ori-F"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
ORIGIN
        1 TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA
       61 GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG
      121 GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA
      181 AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT
      241 ATATGAGTAA ACTTGGTCTG ACAGTTACCA ATGCTTAATC AGTGAGGCAC CTATCTCAGC
      301 GATCTGTCTA TTTCGTTCAT CCATAGTTGC CTGACTCCCC GTCGTGTAGA TAACTACGAT
      361 ACGGGAGGGC TTACCATCTG GCCCCAGTGC TGCAATGATA CCGCGAGAAC CACGCTCACC
      421 GGCTCCAGAT TTATCAGCAA TAAACCAGCC AGCCGGAAGG GCCGAGCGCA GAAGTGGTCC
      481 TGCAACTTTA TCCGCCTCCA TCCAGTCTAT TAATTGTTGC CGGGAAGCTA GAGTAAGTAG
      541 TTCGCCAGTT AATAGTTTGC GCAACGTTGT TGCCATTGCT ACAGGCATCG TGGTGTCACG
      601 CTCGTCGTTT GGTATGGCTT CATTCAGCTC CGGTTCCCAA CGATCAAGGC GAGTTACATG
      661 ATCCCCCATG TTGTGCAAAA AAGCGGTTAG CTCCTTCGGT CCTCCGATCG TTGTCAGAAG
      721 TAAGTTGGCC GCAGTGTTAT CACTCATGGT TATGGCAGCA CTGCATAATT CTCTTACTGT
      781 CATGCCATCC GTAAGATGCT TTTCTGTGAC TGGTGAGTAC TCAACCAAGT CATTCTGAGA
      841 ATAGTGTATG CGGCGACCGA GTTGCTCTTG CCCGGCGTCA ATACGGGATA ATACCGCGCC
      901 ACATAGCAGA ACTTTAAAAG TGCTCATCAT TGGAAAACGT TCTTCGGGGC GAAAACTCTC
      961 AAGGATCTTA CCGCTGTTGA GATCCAGTTC GATGTAACCC ACTCGTGCAC CCAACTGATC
     1021 TTCAGCATCT TTTACTTTCA CCAGCGTTTC TGGGTGAGCA AAAACAGGAA GGCAAAATGC
     1081 CGCAAAAAAG GGAATAAGGG CGACACGGAA ATGTTGAATA CTCATACTCT TCCTTTTTCA
     1141 ATATTATTGA AGCATTTATC AAGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT
     1201 TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACGAATTGG CCAGCGCTGC CATTTTTGGG
     1261 GTGAGGCCGT TCGCGGCCGA GGGGCGCAGC CCCTGGGGGG ATGGGAGGCC CGCGTTAGCG
     1321 GGCCGGGAGG GTTCGAGAAG GGGGGGCACC CCCCTTCGGC GTGCGCGGTC ACGCGCACAG
     1381 GGCGCAGCCC TGGTTAAAAA CAAGGTTTAT AAATATTGGT TTAAAAGCAG GTTAAAAGAC
     1441 AGGTTAGCGG TGGCCGAAAA ACGGGCGGAA ACCCTTGCAA ATGCTGGATT TTCTGCCTGT
     1501 GGACAGCCCC TCAAATGTCA ATAGGTGCGC CCCTCATCTG TCAGCACTCT GCCCCTCAAG
     1561 TGTCAAGGAT CGCGCCCCTC ATCTGTCAGT AGTCGCGCCC CTCAAGTGTC AATACCGCAG
     1621 GGCACTTATC CCCAGGCTTG TCCACATCAT CTGTGGGAAA CTCGCGTAAA ATCAGGCGTT
     1681 TTCGCCGATT TGCGAGGCTG GCCAGCTCCA CGTCGCCGGC CGAAATCGAG CCTGCCCCTC
     1741 ATCTGTCAAC GCCGCGCCGG GTGAGTCGGC CCCTCAAGTG TCAACGTCCG CCCCTCATCT
     1801 GTCAGTGAGG GCCAAGTTTT CCGCGAGGTA TCCACAACGC CGGCGGCCGC GGTGTCTCGC
     1861 ACACGGCTTC GACGGCGTTT CTGGCGCGTT TGCAGGGCCA TAGACGGCCG CCAGCCCAGC
     1921 GGCGAGGGCA ACCAGCCCGG TGAGCGTCGC AAAGGAGATC CTGATCTGAC TGATGGGCTG
     1981 CCTGTATCGA GTGGTGATTT TGTGCCGAGC TGCCGGTCGG GGAGCTGTTG GCTGGCTGGT
     2041 GGCAGGATAT ATTGTGGTGT AAACAAATTG ACGCTTAGAC AACTTAATAA CACATTGCGG
     2101 ACGTTTTTAA TGTACTGGGG TGGATGCAGT GGGCCCCACT CTGTGAAGAC AATGCCGAAT
     2161 TCGGATCCGG AGGAATTCCA ATCCCACAAA AATCTGAGCT TAACAGCACA GTTGCTCCTC
     2221 TCAGAGCAGA ATCGGGTATT CAACACCCTC ATATCAACTA CTACGTTGTG TATAACGGTC
     2281 CACATGCCGG TATATACGAT GACTGGGGTT GTACAAAGGC GGCAACAAAC GGCGTTCCCG
     2341 GAGTTGCACA CAAGAAATTT GCCACTATTA CAGAGGCAAG AGCAGCAGCT GACGCGTACA
     2401 CAACAAGTCA GCAAACAGAC AGGTTGAACT TCATCCCCAA AGGAGAAGCT CAACTCAAGC
     2461 CCAAGAGCTT TGCTAAGGCC CTAACAAGCC CACCAAAGCA AAAAGCCCAC TGGCTCACGC
     2521 TAGGAACCAA AAGGCCCAGC AGTGATCCAG CCCCAAAAGA GATCTCCTTT GCCCCGGAGA
     2581 TTACAATGGA CGATTTCCTC TATCTTTACG ATCTAGGAAG GAAGTTCGAA GGTGAAGGTG
     2641 ACGACACTAT GTTCACCACT GATAATGAGA AGGTTAGCCT CTTCAATTTC AGAAAGAATG
     2701 CTGACCCACA GATGGTTAGA GAGGCCTACG CAGCAAGTCT CATCAAGACG ATCTACCCGA
     2761 GTAACAATCT CCAGGAGATC AAATACCTTC CCAAGAAGGT TAAAGATGCA GTCAAAAGAT
     2821 TCAGGACTAA TTGCATCAAG AACACAGAGA AAGACATATT TCTCAAGATC AGAAGTACTA
     2881 TTCCAGTATG GACGATTCAA GGCTTGCTTC ATAAACCAAG GCAAGTAATA GAGATTGGAG
     2941 TCTCTAAAAA GGTAGTTCCT ACTGAATCTA AGGCCATGCA TGGAGTCTAA GATTCAAATC
     3001 GAGGATCTAA CAGAACTCGC CGTCAAGACT GGCGAACAGT TCATACAGAG TCTTTTACGA
     3061 CTCAATGACA AGAAGAAAAT CTTCGTCAAC ATGGTGGAGC ACGACACTCT GGTCTACTCC
     3121 AAAAATGTCA AAGATACAGT CTCAGAAGAT CAAAGGGCTA TTGAGACTTT TCAACAAAGG
     3181 ATAATTTCGG GAAACCTCCT CGGATTCCAT TGCCCAGCTA TCTGTCACTT CATCGAAAGG
     3241 ACAGTAGAAA AGGAAGGTGG CTCCTACAAA TGCCATCATT GCGATAAAGG AAAGGCTATC
     3301 ATTCAAGATC TCTCTGCCGA CAGTGGTCCC AAAGATGGAC CCCCACCCAC GAGGAGCATC
     3361 GTGGAAAAAG AAGAGGTTCC AACCACGTCT ACAAAGCAAG TGGATTGATG TGACATCTCC
     3421 ACTGACGTAA GGGATGACGC ACAATCCCAC TATCCTTCGC AAGACCCTTC CTCTATATAA
     3481 GGAAGTTCAT TTCATTTGGA GAGGACACGC TCGAGTATAA GAGCTCATTT TTACAACAAT
     3541 TACCAACAAC AACAAACAAC AAACAACATT ACAATTACAT TTACAATTAT CGATACCCAT
     3601 gacaaattct caagcgcttt cacaaccaat tgcctcctct aacgttcatg ataacttcat
     3661 gaataatgaa atcacggcta gtaaaattga tgatggtaat aattcaaaac cactgtcacc
     3721 tggttggacg gaccaaactg cgtataacgc gtttggaatc actacaggga tgtttaatac
     3781 cactacaatg gatgatgtat ataactatct attcgatgat gaagataccc caccaaaacc
     3841 aaaaaaagag ggtggcagtg gagggggcgg aagcggggga atggacacgt atgccggcgc
     3901 ttatgataga caatcaagag aaagagagaa ctcttctgcc gcctctccag ctacacagag
     3961 atctgccaac gaggataaag ctgctgattt acaaagagag gtagagagag atgggggtag
     4021 gtttagattt gtgggtcatt tctctgaggc tcctggtaca tcagcctttg gtactgctga
     4081 acggcctgaa tttgaaagaa tattaaatga gtgtcgggcc ggtcgactaa atatgataat
     4141 agtttatgat gtatctaggt tcagtagact caaagttatg gatgcaattc cgatcgtgag
     4201 cgagctactt gcactcggtg ttactatcgt aagtacacaa gaaggtgtat tcaggcaggg
     4261 taatgtgatg gaccttatac accttattat gcgactcgac gctagccata aagagtcatc
     4321 actgaagagt gctaaaatct tggacaccaa gaacctgcaa agggagcttg gaggctatgt
     4381 aggaggaaaa gctccgtatg gtttcgaact cgtgagtgag acaaaagaga ttaccagaaa
     4441 tggtaggatg gtgaacgtgg ttattaataa gctagctcat agcacaaccc ctctcacagg
     4501 gccttttgag ttcgaacctg acgttattag atggtggtgg agagagatca aaactcacaa
     4561 acatcttcca tttaagccag gtagccaggc tgctatccac cctggatcta tcactgggct
     4621 ttgtaaaagg atggatgctg acgcagtgcc aactcgaggg gaaacaatcg gtaagaaAAC
     4681 Tgcatcttcc gcttgggatc ccgctacagt tatgagaatc cttcgggatc cgcgtattgc
     4741 tggattcgcc gcagaagtca tttataagaa aaaacctgat ggtactccta ctacaaaaat
     4801 cgaaggttat aggatccaaa gggatcctat tactttgcgt ccagttgaat tagactgtgg
     4861 accgatcatc gaacctgccg agtggtacga gttgcaggcc tggcttgatg gtagagggcg
     4921 tggaaagggt ttatctcggg gacaggcaat tttgtcagca atggataagc tttattgtga
     4981 gtgtggggct gttatgacat caaaacgtgg agaagagtca atcaaggatt cttacaggtg
     5041 cagacgtcgt aaagtggtgg acccttctgc tccaggacag catgagggta catgcaacgt
     5101 ttctatggct gctcttgata aatttgtggc cgaacgtatt ttcaacaaga ttagacacgc
     5161 tgaaggggat gaggaaactt tagctcttct ttgggaggct gcccggcgtt ttggtaagct
     5221 aacagaggca ccagaaaaaa gtggcgaaag agctaatttg gtagccgaaa gagcagacgc
     5281 cttgaatgca ttggaagaac tttacgaaga tagggctgcc ggagcatatg atggaccagt
     5341 tggtcggaag catttcagga agcaacaagc tgcactaact ttgagacagc aaggcgcaga
     5401 ggagcgactc gcagagcttg aggccgccga ggccccaaag cttccactcg atcaatggtt
     5461 tcctgaggat gccgacgctg atcctacagg tccaaagagc tggtggggaa gagctagcgt
     5521 agacgataag agagtgtttg tgggtctatt tgttgacaag attgtagtta ctaagtccac
     5581 tacgggtcga ggacagggca ctcccatcga aaagagagcc agtattacct gggcaaagcc
     5641 tccaaccgat gatgacGAGG acgatgcaca agatggtacc gaggatgtgg cagcaccaaa
     5701 aaaaaagagg aaggtgtaag cttCTCTAGC TAGAGTCGAT CGACAAGCTC GAGTTTCTCC
     5761 ATAATAATGT GTGAGTAGTT CCCAGATAAG GGAATTAGGG TTCCTATAGG GTTTCGCTCA
     5821 TGTGTTGAGC ATATAAGAAA CCCTTAGTAT GTATTTGTAT TTGTAAAATA CTTCTATCAA
     5881 TAAAATTTCT AATTCCTAAA ACCAAAATCC AGTACTAAAA TCCAGATCGC TGCAATTGTC
     5941 TTCTGCACGA AGTGGTTTAA ACTATCAGTG TTTGACAGGA TATATTGGCG GGTAAACCTA
     6001 AGAGAAAAGA GCGTTTATTA GAATAATCGG ATATTTAAAA GGGCGTGAAA AGGTTTATCC
     6061 GTTCGTCCAT TTGTATGTGC ATGCCAACCA CAGGGTTCCC CAGATCAGGC GCTGGCTGCT
     6121 GAACCCCCAG CCGGAACTGA CCCCACAAGG CCCTAGCGTT TGCAATGCAC CAGGTCATCA
     6181 TTGACCCAGG CGTGTTCCAC CAGGCCGCTG CCTCGCAACT CTTCGCAGGC TTCGCCGACC
     6241 TGCTCGCGCC ACTTCTTCAC GCGGGTGGAA TCCGATCCGC ACATGAGGCG GAAGGTTTCC
     6301 AGCTTGAGCG GGTACGGCTC CCGGTGCGAG CTGAAATAGT CGAACATCCG TCGGGCCGTC
     6361 GGCGACAGCT TGCGGTACTT CTCCCATATG AATTTCGTGT AGTGGTCGCC AGCAAACAGC
     6421 ACGACGATTT CCTCGTCGAT CAGGACCTGG CAACGGGACG TTTTCTTGCC ACGGTCCAGG
     6481 ACGCGGAAGC GGTGCAGCAG CGACACCGAT TCCAGGTGCC CAACGCGGTC GGACGTGAAG
     6541 CCCATCGCCG TCGCCTGTAG GCGCGACAGG CATTCCTCGG CCTTCGTGTA ATACCGGCCA
     6601 TTGATCGACC AGCCCAGGTC CTGGCAAAGC TCGTAGAACG TGAAGGTGAT CGGCTCGCCG
     6661 ATAGGGGTGC GCTTCGCGTA CTCCAACACC TGCTGCCACA CCAGTTCGTC ATCGTCGGCC
     6721 CGCAGCTCGA CGCCGGTGTA GGTGATCTTC ACGTCCTTGT TGACGTGGAA AATGACCTTG
     6781 TTTTGCAGCG CCTCGCGCGG GATTTTCTTG TTGCGCGTGG TGAACAGGGC AGAGCGGGCC
     6841 GTGTCGTTTG GCATCGCTCG CATCGTGTCC GGCCACGGCG CAATATCGAA CAAGGAAAGC
     6901 TGCATTTCCT TGATCTGCTG CTTCGTGTGT TTCAGCAACG CGGCCTGCTT GGCCTCGCTG
     6961 ACCTGTTTTG CCAGGTCCTC GCCGGCGGTT TTTCGCTTCT TGGTCGTCAT AGTTCCTCGC
     7021 GTGTCGATGG TCATCGACTT CGCCAAACCT GCCGCCTCCT GTTCAAGACG ACGCGAACGC
     7081 TCCACGGCGG CCGATGGCGC GGGCAGGGCA GGGGGAGCCA GTTGCACGCT GTCGCGCTCG
     7141 ATCTTGGCCG TAGCTTGCTG GACCATCGAG CCGACGGACT GGAAGGTTTC GCGGGGCGCA
     7201 CGCATGACGG TGCGGCTTGC GATGGTTTCG GCATCCTCGG CGGAAAACCC CGCGTCGATC
     7261 AGTTCTTGCC TGTATGCCTT CCGGTCAAAC GTCCGATTCA TTCACCCTCC TTGCGGGATT
     7321 GCCCCGACTC ACGCCGGGGC AATGTGCCCT TATTCCTGAT TTGACCCGCC TGGTGCCTTG
     7381 GTGTCCAGAT AATCCACCTT ATCGGCAATG AAGTCGGTCC CGTAGACCGT CTGGCCGTCC
     7441 TTCTCGTACT TGGTATTCCG AATCTTGCCC TGCACGAATA CCAGCGACCC CTTGCCCAAA
     7501 TACTTGCCGT GGGCCTCGGC CTGAGAGCCA AAACACTTGA TGCGGAAGAA GTCGGTGCGC
     7561 TCCTGCTTGT CGCCGGCATC GTTGCGCCAC ATCTAGGATC TGCCAGGAAC CGTAAAAAGG
     7621 CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC
     7681 GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG
     7741 GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT
     7801 TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG
     7861 TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT
     7921 GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC
     7981 TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT
     8041 TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC AGTATTTGGT ATCTGCGCTC
     8101 TGCTGAAGCC AGTTACCTTC GGAAAAAGAG T
//