LOCUS       pEPQD1CB0784_(P1_nosP-T 5510 bp ds-DNA     circular     15-OCT-2020
DEFINITION  .
FEATURES             Location/Qualifiers
     Misc._feature   10..157
                     /label="LB"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     primer          22..44
                     /label="QMD671_GB_LB_seq_F"
                     /note="sequence: ctgtatcgagtggtgattttgtg"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer          76..99
                     /label="214or (L1, REV, LB)"
                     /note="sequence: CTGGTGGCAGGATATATTGTGGTG"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    complement(80..104)
                     /label="LB T-DNA repeat"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     primer          94..114
                     /label="226or (L2, FWD, LB)"
                     /note="sequence: GTGGTGTAAACAAATTGACGC"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     primer          complement(94..115)
                     /label="QMD541_LB_rev_seq(226or_binding)"
                     /note="sequence: AGCGTCAATTTGTTTACACCAC"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     Promoter        213..488
                     /label="nos-p"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     primer          323..347
                     /label="QMD230_pTRV1oursideseq_R"
                     /note="sequence: gtttctggagtttaatgagctaagc"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    493..559
                     /label="TMV omega"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     CDS             561..1079
                     /label="P19 (TBSV)"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     CDS             561..1079
                     /label="Translation 561-1079"
     Terminator      1084..1346
                     /label="nost"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     primer          1320..1343
                     /label="QMD327_nosT_seq_R"
                     /note="sequence: GCGGTGTCATCTATGTTACTAGAT"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     Misc._feature   complement(1378..1505)
                     /label="RB short"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    complement(1392..1416)
                     /label="RB T-DNA repeat"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     primer          complement(1410..1434)
                     /label="QMD315_LB_Level2/M_seq_R"
                     /note="sequence: aacgctcttttctcttaggtttacc"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     primer          complement(1460..1479)
                     /label="227or (L2, REV, RB)"
                     /note="sequence: GGATAAACCTTTTCACGCCC"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     conflict        1481..1499
                     /label="partial overdrive"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     primer          complement(1492..1517)
                     /label="213or (L1, FWD, RB)"
                     /note="sequence: GAACCCTGTGGTTGGCATGCACATAC"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     Origin          complement(1529..3010)
                     /label="RK2"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     primer          complement(1572..1590)
                     /label="QMD314_TrfA_LMoutside_F"
                     /note="sequence: ggtgcattgcaaacgctag"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     CDS             complement(1572..2720)
                     /label="Plasmid replication initiator protein TrfA, P07676 (TRFA_ECOLX)"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    complement(3041..3655)
                     /label="replicon of pMB1"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     rep_origin      complement(3041..3660)
                     /label="pBR322\(from\pUC18)"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     primer          complement(3101..3119)
                     /label="QMD062_pJL1_outside(BR322)_F"
                     /note="sequence: tcgccacctctgacttgag"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer          complement(3103..3122)
                     /label="QMD132_LETprom2_F, 61.4"
                     /note="sequence: gtttcgccacctctgacttg"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer          complement(3132..3155)
                     /label="QMD131_LETprom1_F, 61.7, -3.89 self"
                     /note="sequence: gggaaacgcctggtatctttatag"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     primer_bind     complement(3136..3155)
                     /label="pBR322ori-F"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     primer          3601..3622
                     /label="QMD540_pBR322_seq_R"
                     /note="sequence: ttgtttgcaagcagcagattac"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     CDS             complement(3815..4675)
                     /label="AmpR"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer          complement(3942..3958)
                     /label="QMD047 binding region (57.3)"
                     /note="sequence: TCTCGCGGTATCATTGC"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     promoter        complement(4676..4780)
                     /label="AmpR promoter"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     primer          4720..4747
                     /label="212or (L0 REV)"
                     /note="sequence: GTCTCATGAGCGGATACATATTTGAATG"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     rep_origin      complement(4867..5498)
                     /label="oriV"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
ORIGIN
        1 CTGATCTGAC TGATGGGCTG CCTGTATCGA GTGGTGATTT TGTGCCGAGC TGCCGGTCGG
       61 GGAGCTGTTG GCTGGCTGGT GGCAGGATAT ATTGTGGTGT AAACAAATTG ACGCTTAGAC
      121 AACTTAATAA CACATTGCGG ACGTTTTTAA TGTACTGGGG TGGATGCAGT GGGCCCCACT
      181 CTGTGAAGAC AATGCCGAAT TCGGATCCGG AGAGCGGAGA ATTAAGGGAG TCACGTTATG
      241 ACCCCCGCCG ATGACGCGGG ACAAGCCGTT TTACGTTTGG AACTGACAGA ACCGCAACGT
      301 TGAAGGAGCC ACTCAGCCGC GGGTTTCTGG AGTTTAATGA GCTAAGCACA TACGTCAGAA
      361 ACCATTATTG CGCGTTCAAA AGTCGCCTAA GGTCACTATC AGCTAGCAAA TATTTCTTGT
      421 CAAAAATGCT CCACTGACGT TCCATAAATT CCCCTCGGTA TCCAATTAGA GTCTCATATT
      481 CACTCTCATA CTGTATTTTT ACAACAATTA CCAACAACAA CAAACAACAA ACAACATTAC
      541 AATTACTATT TACAATTACA ATGGAACGAG CTATACAAGG AAACGATGCT AGGGAACAAG
      601 CTTATGGTGA ACGTTGGAAT GGAGGATCAG GAAGTTCCAC TTCTCCCTTC AAACTTCCTG
      661 ACGAAAGTCC GAGTTGGACT GAGTGGCGGC TACATAACGA TGAGACTATT TCGAATCAAG
      721 ATAATCCCCT TGGTTTCAAG GAAAGCTGGG GTTTCGGGAA AGTTGTATTT AAGAGATATC
      781 TCAGATACGA CGGGACGGAA ACTTCACTGC ACAGAGTCCT TGGATCTTGG ACGGGAGATT
      841 CGGTTAACTA TGCAGCATCT CGATTTCTCG GTTTCGACCA GATCGGATGT ACCTATAGTA
      901 TTCGGTTTCG AGGAGTTAGT GTCACCATTT CTGGAGGGTC GCGAACTCTT CAGCATCTCA
      961 GTGAAATGGC AATTCGGTCT AAGCAAGAAC TGCTACAGCT TACCCCAGTC AAAGTGGAAA
     1021 GTGATGTATC AAGAGGATGC CCTGAAGGTG TTGAAACCTT CGAAGAAGAA AGCGAGTAAG
     1081 CTTGTCAAGC AGATCGTTCA AACATTTGGC AATAAAGTTT CTTAAGATTG AATCCTGTTG
     1141 CCGGTCTTGC GATGATTATC ATATAATTTC TGTTGAATTA CGTTAAGCAT GTAATAATTA
     1201 ACATGTAATG CATGACGTTA TTTATGAGAT GGGTTTTTAT GATTAGAGTC CCGCAATTAT
     1261 ACATTTAATA CGCGATAGAA AACAAAATAT AGCGCGCAAA CTAGGATAAA TTATCGCGCG
     1321 CGGTGTCATC TATGTTACTA GATCGACGCT GCAATTGTCT TCTGCACGAA GTGGTTTAAA
     1381 CTATCAGTGT TTGACAGGAT ATATTGGCGG GTAAACCTAA GAGAAAAGAG CGTTTATTAG
     1441 AATAATCGGA TATTTAAAAG GGCGTGAAAA GGTTTATCCG TTCGTCCATT TGTATGTGCA
     1501 TGCCAACCAC AGGGTTCCCC AGATCAGGCG CTGGCTGCTG AACCCCCAGC CGGAACTGAC
     1561 CCCACAAGGC CCTAGCGTTT GCAATGCACC AGGTCATCAT TGACCCAGGC GTGTTCCACC
     1621 AGGCCGCTGC CTCGCAACTC TTCGCAGGCT TCGCCGACCT GCTCGCGCCA CTTCTTCACG
     1681 CGGGTGGAAT CCGATCCGCA CATGAGGCGG AAGGTTTCCA GCTTGAGCGG GTACGGCTCC
     1741 CGGTGCGAGC TGAAATAGTC GAACATCCGT CGGGCCGTCG GCGACAGCTT GCGGTACTTC
     1801 TCCCATATGA ATTTCGTGTA GTGGTCGCCA GCAAACAGCA CGACGATTTC CTCGTCGATC
     1861 AGGACCTGGC AACGGGACGT TTTCTTGCCA CGGTCCAGGA CGCGGAAGCG GTGCAGCAGC
     1921 GACACCGATT CCAGGTGCCC AACGCGGTCG GACGTGAAGC CCATCGCCGT CGCCTGTAGG
     1981 CGCGACAGGC ATTCCTCGGC CTTCGTGTAA TACCGGCCAT TGATCGACCA GCCCAGGTCC
     2041 TGGCAAAGCT CGTAGAACGT GAAGGTGATC GGCTCGCCGA TAGGGGTGCG CTTCGCGTAC
     2101 TCCAACACCT GCTGCCACAC CAGTTCGTCA TCGTCGGCCC GCAGCTCGAC GCCGGTGTAG
     2161 GTGATCTTCA CGTCCTTGTT GACGTGGAAA ATGACCTTGT TTTGCAGCGC CTCGCGCGGG
     2221 ATTTTCTTGT TGCGCGTGGT GAACAGGGCA GAGCGGGCCG TGTCGTTTGG CATCGCTCGC
     2281 ATCGTGTCCG GCCACGGCGC AATATCGAAC AAGGAAAGCT GCATTTCCTT GATCTGCTGC
     2341 TTCGTGTGTT TCAGCAACGC GGCCTGCTTG GCCTCGCTGA CCTGTTTTGC CAGGTCCTCG
     2401 CCGGCGGTTT TTCGCTTCTT GGTCGTCATA GTTCCTCGCG TGTCGATGGT CATCGACTTC
     2461 GCCAAACCTG CCGCCTCCTG TTCAAGACGA CGCGAACGCT CCACGGCGGC CGATGGCGCG
     2521 GGCAGGGCAG GGGGAGCCAG TTGCACGCTG TCGCGCTCGA TCTTGGCCGT AGCTTGCTGG
     2581 ACCATCGAGC CGACGGACTG GAAGGTTTCG CGGGGCGCAC GCATGACGGT GCGGCTTGCG
     2641 ATGGTTTCGG CATCCTCGGC GGAAAACCCC GCGTCGATCA GTTCTTGCCT GTATGCCTTC
     2701 CGGTCAAACG TCCGATTCAT TCACCCTCCT TGCGGGATTG CCCCGACTCA CGCCGGGGCA
     2761 ATGTGCCCTT ATTCCTGATT TGACCCGCCT GGTGCCTTGG TGTCCAGATA ATCCACCTTA
     2821 TCGGCAATGA AGTCGGTCCC GTAGACCGTC TGGCCGTCCT TCTCGTACTT GGTATTCCGA
     2881 ATCTTGCCCT GCACGAATAC CAGCGACCCC TTGCCCAAAT ACTTGCCGTG GGCCTCGGCC
     2941 TGAGAGCCAA AACACTTGAT GCGGAAGAAG TCGGTGCGCT CCTGCTTGTC GCCGGCATCG
     3001 TTGCGCCACA TCTAGGATCT GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC
     3061 ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA
     3121 ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC
     3181 CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG
     3241 CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC
     3301 TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC
     3361 GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA
     3421 GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT
     3481 ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG
     3541 GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT
     3601 TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT
     3661 TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA
     3721 GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA
     3781 TCTAAAGTAT ATATGAGTAA ACTTGGTCTG ACAGTTACCA ATGCTTAATC AGTGAGGCAC
     3841 CTATCTCAGC GATCTGTCTA TTTCGTTCAT CCATAGTTGC CTGACTCCCC GTCGTGTAGA
     3901 TAACTACGAT ACGGGAGGGC TTACCATCTG GCCCCAGTGC TGCAATGATA CCGCGAGAAC
     3961 CACGCTCACC GGCTCCAGAT TTATCAGCAA TAAACCAGCC AGCCGGAAGG GCCGAGCGCA
     4021 GAAGTGGTCC TGCAACTTTA TCCGCCTCCA TCCAGTCTAT TAATTGTTGC CGGGAAGCTA
     4081 GAGTAAGTAG TTCGCCAGTT AATAGTTTGC GCAACGTTGT TGCCATTGCT ACAGGCATCG
     4141 TGGTGTCACG CTCGTCGTTT GGTATGGCTT CATTCAGCTC CGGTTCCCAA CGATCAAGGC
     4201 GAGTTACATG ATCCCCCATG TTGTGCAAAA AAGCGGTTAG CTCCTTCGGT CCTCCGATCG
     4261 TTGTCAGAAG TAAGTTGGCC GCAGTGTTAT CACTCATGGT TATGGCAGCA CTGCATAATT
     4321 CTCTTACTGT CATGCCATCC GTAAGATGCT TTTCTGTGAC TGGTGAGTAC TCAACCAAGT
     4381 CATTCTGAGA ATAGTGTATG CGGCGACCGA GTTGCTCTTG CCCGGCGTCA ATACGGGATA
     4441 ATACCGCGCC ACATAGCAGA ACTTTAAAAG TGCTCATCAT TGGAAAACGT TCTTCGGGGC
     4501 GAAAACTCTC AAGGATCTTA CCGCTGTTGA GATCCAGTTC GATGTAACCC ACTCGTGCAC
     4561 CCAACTGATC TTCAGCATCT TTTACTTTCA CCAGCGTTTC TGGGTGAGCA AAAACAGGAA
     4621 GGCAAAATGC CGCAAAAAAG GGAATAAGGG CGACACGGAA ATGTTGAATA CTCATACTCT
     4681 TCCTTTTTCA ATATTATTGA AGCATTTATC AAGGTTATTG TCTCATGAGC GGATACATAT
     4741 TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACGAATTGG CCAGCGCTGC
     4801 CATTTTTGGG GTGAGGCCGT TCGCGGCCGA GGGGCGCAGC CCCTGGGGGG ATGGGAGGCC
     4861 CGCGTTAGCG GGCCGGGAGG GTTCGAGAAG GGGGGGCACC CCCCTTCGGC GTGCGCGGTC
     4921 ACGCGCACAG GGCGCAGCCC TGGTTAAAAA CAAGGTTTAT AAATATTGGT TTAAAAGCAG
     4981 GTTAAAAGAC AGGTTAGCGG TGGCCGAAAA ACGGGCGGAA ACCCTTGCAA ATGCTGGATT
     5041 TTCTGCCTGT GGACAGCCCC TCAAATGTCA ATAGGTGCGC CCCTCATCTG TCAGCACTCT
     5101 GCCCCTCAAG TGTCAAGGAT CGCGCCCCTC ATCTGTCAGT AGTCGCGCCC CTCAAGTGTC
     5161 AATACCGCAG GGCACTTATC CCCAGGCTTG TCCACATCAT CTGTGGGAAA CTCGCGTAAA
     5221 ATCAGGCGTT TTCGCCGATT TGCGAGGCTG GCCAGCTCCA CGTCGCCGGC CGAAATCGAG
     5281 CCTGCCCCTC ATCTGTCAAC GCCGCGCCGG GTGAGTCGGC CCCTCAAGTG TCAACGTCCG
     5341 CCCCTCATCT GTCAGTGAGG GCCAAGTTTT CCGCGAGGTA TCCACAACGC CGGCGGCCGC
     5401 GGTGTCTCGC ACACGGCTTC GACGGCGTTT CTGGCGCGTT TGCAGGGCCA TAGACGGCCG
     5461 CCAGCCCAGC GGCGAGGGCA ACCAGCCCGG TGAGCGTCGC AAAGGAGATC
//