LOCUS       pEPQD1CB0785_(P2_4X_opa 7294 bp ds-DNA     circular     05-NOV-2020
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    complement(26..176)
                     /label="LB"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    complement(95..119)
                     /label="LB T-DNA repeat"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    228..336
                     /label="OpattB"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    228..341
                     /label="MinSyn_068"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    259..282
                     /label="repeat seq"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    318..341
                     /label="repeat"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    346..349
                     /label="additional sites for pcr"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    350..458
                     /label="OpattB"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    381..404
                     /label="repeat seq"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    440..463
                     /label="repeat"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    463..511
                     /label="min 35S"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    519..539
                     /label="part of TMV 5'UTR"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     5'_UTR          592..674
                     /label="TMV omega"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     Transit_peptide 675..850
                     /label="chloroplast targeting signal"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_feature    855..2891
                     /label="CrDXS2 (no transit peptide, domesticated, 1 BsaI site removed) amplified from NS-I-132A2 V2, similar to DQ848672.1 but contains R414K, T533A, A592T"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     Terminator      2896..3145
                     /label="Atu mas terminator from pICH77901"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    complement(3173..3298)
                     /label="RB"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    complement(3191..3215)
                     /label="RB T-DNA repeat"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     conflict        3280..3298
                     /label="partial overdrive"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     Origin          complement(3328..4809)
                     /label="RK2"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     CDS             complement(3371..4519)
                     /label="Plasmid replication initiator protein TrfA, P07676 (TRFA_ECOLX)"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     primer_bind     complement(4935..4954)
                     /label="pBR322ori-F"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     CDS             complement(5614..6474)
                     /label="AmpR"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     rep_origin      complement(join(6666..7294,1..3))
                     /label="oriV"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
ORIGIN
        1 GTCGCAAAGG AGATCCTGAT CTGACTGATG GGCTGCCTGT ATCGAGTGGT GATTTTGTGC
       61 CGAGCTGCCG GTCGGGGAGC TGTTGGCTGG CTGGTGGCAG GATATATTGT GGTGTAAACA
      121 AATTGACGCT TAGACAACTT AATAACACAT TGCGGACGTT TTTAATGTAC TGGGGTGGAT
      181 GCAGTGGGCC CCACTCTGTG AAGACAAGCA AGAATTCAAG CTTGGAGcgg tgcgggtgcc
      241 agggcgtgcc cttgggctcc ccgggcgcgt actccactcc ccgagccggt gcgggtgcca
      301 gggcgtgccc ttgggctccc cgggcgcgta ctccactccc cCCATggtgc ggtgcgggtg
      361 ccagggcgtg cccttgggct ccccgggcgc gtactccact ccccgagccg gtgcgggtgc
      421 cagggcgtgc ccttgggctc cccgggcgcg tactccactc cccttcgcaa gacccttcct
      481 ctatataagg aagttcattt catttggaga ggactccggt atttttacaa caattaccac
      541 aacaaaacaa acaacaaaca acattacaat ttactattct agtcgaaTAC TGTATAAGAG
      601 CTCATTTTTA CAACAATTAC CAACAACAAC AAACAACAAA CAACATTACA ATTACATTTA
      661 CAATTATCGA TAACATGGCT TCTTCTATGC TTTCTTCTGC TGCTGTTGTT GCTACTCGTG
      721 CTAGTGCTGC TCAAGCTAGT ATGGTTGCTC CTTTTACTGG ACTTAAGTCT GCTGCTTCTT
      781 TTCCTGTTAC TAGAAAGCAA AACAACCTTG ATATTACTTC TATTGCTAGT AACGGAGGAA
      841 GAGTTCGAGC AATGGCTGCT GCTCATGGGC ATGCTCATAA TGTTGATGAA AATTACCAAG
      901 TCCTAGTGAA GAAGCAGCCT TTGAATTTTT CAGGAGATAA GCCTTTTACT CCTATTTTGG
      961 ATACCATCAA CTACCCTACC CATATGAAGA ATCTCTCTCT TCAGGAGCTT CAACAATTAG
     1021 CTGACGAATT AAGGGAGGAG ATAGTGTATT CTGTGGCCAA AACGGGTGGT CACTTAAGTT
     1081 CCAGCCTAGG TGTGGCCGAG CTCACTGTGG CACTCCACCA CGTGTTCGAA ACCCCAAATG
     1141 ATAAAATCAT ATGGGATGTT GGCCACCAGA CATATGGGCA TAAGATTCTG ACAGGGAGAA
     1201 GGTCAAAGAT GCATACCATG AGACAAACTT GCGGACTTGC AGGTTTCCCC AAAAGAGATG
     1261 AAAGTGCCCA TGATGCATTT GGAGTTGGCC ATAGTTCCAC CAGCATTTCT GCCGGTTTAG
     1321 GAATGGCAAT AGGACGAGAT TTATTAGGTA AGAAAAATCA TGTAATAACG GTAATAGGAG
     1381 ATGGAGCTAT GACAGCCGGA CAAGCATATG AAGCCATGAA TAATGCTGGA TATCTTGATT
     1441 CTAATCTCAT AATCATATTG AATGATAACA AACAAGTTTC TCTCCCGACT GCCACAATTG
     1501 ATGGTCCTGC ACCTCCTACT GGTGCTCTAA GCAATGCCTT AACTAGGCTT CAATCTAGTA
     1561 GAAAATTTCG ACTACTTCGG GAGGCTGCAA AAGGTGTGAC AAAGCAATTA GGGGGACAAG
     1621 TTCATGAAAT GGCAGCTAAA GTGGATACAT ATATGAGAGG GATGAATAGC CAAAGGGCTT
     1681 CCTTATTTGA AGATTTAGGA ATGTATTATA TTGGTCCACT TGATGGTCAC AATATGGAAG
     1741 ATCTTGTTGA TATTTTAGAG AAAGTAAAAT CTGTTCCTGC AACGGGCCCT GTTCTTATTC
     1801 ATATTATTAC TGAGAAAGGA AAAGGATATC CTCCTGCTGA AGCTGCCGCC GACAAAATGC
     1861 ATGGGGTGGT GAAATTTGAG CCAATGACAG GAAAACAATT CAAGTCAAAG TCAAAAACAA
     1921 AGTCATATAC AAATTATTTT GCTGAGAGTT TAATAAGTGA AGCTAGGGAA GATGATAAGA
     1981 TTATTGCCAT TCATGCTGCT ATGGGAGGAG GAACAGGACT CAATTTATTC CAGAAACACT
     2041 TTCCTGAACG ATGCTTTGAT GTTGGGATAG CTGAGCAACA TGCTGTCACT TTTGCTGCTG
     2101 GCTTAGCTTC TGAAGGACTC AAGCCTTTTT GTGCTATTTA CTCATCTTTC TTACAAAGAG
     2161 GTTATGATCA GGTGGTTCAT GATGTGGACC TTCAAAAAAT TCCAGTAAGG TTTGCTATGG
     2221 ATAGAGCAGG GCTGGTGGGT GCAGATGGAC CCACTCATTG CGGTGCATTT GACACAACTT
     2281 TTATGGCTTG TTTGCCTAAT ATGGTGGTCA TGGCTCCTTC AGATGAAACC GAATTGATGC
     2341 ACATGGTTGC CACAGCAGCT GCCATTGATG ATAGGCCTAG TTGCTTTCGA TATCCCAGAG
     2401 GAAATGGCAT TGGTGCAATT CTTCCACCAA ACAACAAAGG AACTCCATTA CAGATTGGTA
     2461 AAGGAAGAAT ATTGTTGGAG GGAACAAGAG TTGCCATTCT TGGGTATGGA GCAATGGTGC
     2521 AAAACTGTTT AATGGCTGCT CAGCTTCTTG AAATTAATGG AATATCAGCT ACAGTTGTTG
     2581 ATGCCCGATT TTGCAAACCT CTTGATGGAG ATTTGATTAG AAAATTGGCT CAACAACATG
     2641 AAGTCCTTAT CACTGTTGAA GAAGGATCCA TTGGTGGTTT TGGTTCCCAT GTGGCACAGT
     2701 TCTTGGCTTT GAATGGATTG CTTGATGGAA ATTTAAAGTG GAGGGCAATG ATGCTTCCTG
     2761 ACAGATATAT TGATCATGGA GCTCAAAATG ACCAAATTGA AGAGGCTGGC CTAACTCCAA
     2821 AGCATATTGC TGGCACTGTG TTTTCTCTAC TCAGCAAAAA CAAGAATACT CTTCAAGTTC
     2881 TCCAGTTTTA GGCTTGGACT CCCATGTTGG CAAAGGCAAC CAAACAAACA ATGAATGATC
     2941 CGCTCCTGCA TATGGGGCGG TTTGAGTATT TCAACTGCCA TTTGGGCTGA ATTGTAGACA
     3001 TGCTCCTGTC AGAAATTCCG TGATCTTACT CAATATTCAG TAATCTCGGC CAATATCCTA
     3061 AATGTGCGTG GCTTTATCTG TCTTTGTATT GTTTCATCAA TTCATGTAAC GTTTGCTTTT
     3121 CTTATGAATT TTCAAATAAA TTATCCGCTA CTATTGTCTT CTGCACGAAG TGGTTTAAAC
     3181 TATCAGTGTT TGACAGGATA TATTGGCGGG TAAACCTAAG AGAAAAGAGC GTTTATTAGA
     3241 ATAATCGGAT ATTTAAAAGG GCGTGAAAAG GTTTATCCGT TCGTCCATTT GTATGTGCAT
     3301 GCCAACCACA GGGTTCCCCA GATCAGGCGC TGGCTGCTGA ACCCCCAGCC GGAACTGACC
     3361 CCACAAGGCC CTAGCGTTTG CAATGCACCA GGTCATCATT GACCCAGGCG TGTTCCACCA
     3421 GGCCGCTGCC TCGCAACTCT TCGCAGGCTT CGCCGACCTG CTCGCGCCAC TTCTTCACGC
     3481 GGGTGGAATC CGATCCGCAC ATGAGGCGGA AGGTTTCCAG CTTGAGCGGG TACGGCTCCC
     3541 GGTGCGAGCT GAAATAGTCG AACATCCGTC GGGCCGTCGG CGACAGCTTG CGGTACTTCT
     3601 CCCATATGAA TTTCGTGTAG TGGTCGCCAG CAAACAGCAC GACGATTTCC TCGTCGATCA
     3661 GGACCTGGCA ACGGGACGTT TTCTTGCCAC GGTCCAGGAC GCGGAAGCGG TGCAGCAGCG
     3721 ACACCGATTC CAGGTGCCCA ACGCGGTCGG ACGTGAAGCC CATCGCCGTC GCCTGTAGGC
     3781 GCGACAGGCA TTCCTCGGCC TTCGTGTAAT ACCGGCCATT GATCGACCAG CCCAGGTCCT
     3841 GGCAAAGCTC GTAGAACGTG AAGGTGATCG GCTCGCCGAT AGGGGTGCGC TTCGCGTACT
     3901 CCAACACCTG CTGCCACACC AGTTCGTCAT CGTCGGCCCG CAGCTCGACG CCGGTGTAGG
     3961 TGATCTTCAC GTCCTTGTTG ACGTGGAAAA TGACCTTGTT TTGCAGCGCC TCGCGCGGGA
     4021 TTTTCTTGTT GCGCGTGGTG AACAGGGCAG AGCGGGCCGT GTCGTTTGGC ATCGCTCGCA
     4081 TCGTGTCCGG CCACGGCGCA ATATCGAACA AGGAAAGCTG CATTTCCTTG ATCTGCTGCT
     4141 TCGTGTGTTT CAGCAACGCG GCCTGCTTGG CCTCGCTGAC CTGTTTTGCC AGGTCCTCGC
     4201 CGGCGGTTTT TCGCTTCTTG GTCGTCATAG TTCCTCGCGT GTCGATGGTC ATCGACTTCG
     4261 CCAAACCTGC CGCCTCCTGT TCAAGACGAC GCGAACGCTC CACGGCGGCC GATGGCGCGG
     4321 GCAGGGCAGG GGGAGCCAGT TGCACGCTGT CGCGCTCGAT CTTGGCCGTA GCTTGCTGGA
     4381 CCATCGAGCC GACGGACTGG AAGGTTTCGC GGGGCGCACG CATGACGGTG CGGCTTGCGA
     4441 TGGTTTCGGC ATCCTCGGCG GAAAACCCCG CGTCGATCAG TTCTTGCCTG TATGCCTTCC
     4501 GGTCAAACGT CCGATTCATT CACCCTCCTT GCGGGATTGC CCCGACTCAC GCCGGGGCAA
     4561 TGTGCCCTTA TTCCTGATTT GACCCGCCTG GTGCCTTGGT GTCCAGATAA TCCACCTTAT
     4621 CGGCAATGAA GTCGGTCCCG TAGACCGTCT GGCCGTCCTT CTCGTACTTG GTATTCCGAA
     4681 TCTTGCCCTG CACGAATACC AGCGACCCCT TGCCCAAATA CTTGCCGTGG GCCTCGGCCT
     4741 GAGAGCCAAA ACACTTGATG CGGAAGAAGT CGGTGCGCTC CTGCTTGTCG CCGGCATCGT
     4801 TGCGCCACAT CTAGGATCTG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA
     4861 TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA
     4921 CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC
     4981 TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC
     5041 GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT
     5101 GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG
     5161 TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG
     5221 GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA
     5281 CGGCTACACT AGAAGGACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG
     5341 AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT
     5401 TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT
     5461 TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG
     5521 ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT
     5581 CTAAAGTATA TATGAGTAAA CTTGGTCTGA CAGTTACCAA TGCTTAATCA GTGAGGCACC
     5641 TATCTCAGCG ATCTGTCTAT TTCGTTCATC CATAGTTGCC TGACTCCCCG TCGTGTAGAT
     5701 AACTACGATA CGGGAGGGCT TACCATCTGG CCCCAGTGCT GCAATGATAC CGCGAGAACC
     5761 ACGCTCACCG GCTCCAGATT TATCAGCAAT AAACCAGCCA GCCGGAAGGG CCGAGCGCAG
     5821 AAGTGGTCCT GCAACTTTAT CCGCCTCCAT CCAGTCTATT AATTGTTGCC GGGAAGCTAG
     5881 AGTAAGTAGT TCGCCAGTTA ATAGTTTGCG CAACGTTGTT GCCATTGCTA CAGGCATCGT
     5941 GGTGTCACGC TCGTCGTTTG GTATGGCTTC ATTCAGCTCC GGTTCCCAAC GATCAAGGCG
     6001 AGTTACATGA TCCCCCATGT TGTGCAAAAA AGCGGTTAGC TCCTTCGGTC CTCCGATCGT
     6061 TGTCAGAAGT AAGTTGGCCG CAGTGTTATC ACTCATGGTT ATGGCAGCAC TGCATAATTC
     6121 TCTTACTGTC ATGCCATCCG TAAGATGCTT TTCTGTGACT GGTGAGTACT CAACCAAGTC
     6181 ATTCTGAGAA TAGTGTATGC GGCGACCGAG TTGCTCTTGC CCGGCGTCAA TACGGGATAA
     6241 TACCGCGCCA CATAGCAGAA CTTTAAAAGT GCTCATCATT GGAAAACGTT CTTCGGGGCG
     6301 AAAACTCTCA AGGATCTTAC CGCTGTTGAG ATCCAGTTCG ATGTAACCCA CTCGTGCACC
     6361 CAACTGATCT TCAGCATCTT TTACTTTCAC CAGCGTTTCT GGGTGAGCAA AAACAGGAAG
     6421 GCAAAATGCC GCAAAAAAGG GAATAAGGGC GACACGGAAA TGTTGAATAC TCATACTCTT
     6481 CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT
     6541 TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC ACGAATTGGC CAGCGCTGCC
     6601 ATTTTTGGGG TGAGGCCGTT CGCGGCCGAG GGGCGCAGCC CCTGGGGGGA TGGGAGGCCC
     6661 GCGTTAGCGG GCCGGGAGGG TTCGAGAAGG GGGGGCACCC CCCTTCGGCG TGCGCGGTCA
     6721 CGCGCACAGG GCGCAGCCCT GGTTAAAAAC AAGGTTTATA AATATTGGTT TAAAAGCAGG
     6781 TTAAAAGACA GGTTAGCGGT GGCCGAAAAA CGGGCGGAAA CCCTTGCAAA TGCTGGATTT
     6841 TCTGCCTGTG GACAGCCCCT CAAATGTCAA TAGGTGCGCC CCTCATCTGT CAGCACTCTG
     6901 CCCCTCAAGT GTCAAGGATC GCGCCCCTCA TCTGTCAGTA GTCGCGCCCC TCAAGTGTCA
     6961 ATACCGCAGG GCACTTATCC CCAGGCTTGT CCACATCATC TGTGGGAAAC TCGCGTAAAA
     7021 TCAGGCGTTT TCGCCGATTT GCGAGGCTGG CCAGCTCCAC GTCGCCGGCC GAAATCGAGC
     7081 CTGCCCCTCA TCTGTCAACG CCGCGCCGGG TGAGTCGGCC CCTCAAGTGT CAACGTCCGC
     7141 CCCTCATCTG TCAGTGAGGG CCAAGTTTTC CGCGAGGTAT CCACAACGCC GGCGGCCGCG
     7201 GTGTCTCGCA CACGGCTTCG ACGGCGTTTC TGGCGCGTTT GCAGGGCCAT AGACGGCCGC
     7261 CAGCCCAGCG GCGAGGGCAA CCAGCCCGGT GAGC
//