LOCUS       pEPQD1CB0790_(P1_4X_opa 6183 bp ds-DNA     circular     14-OCT-2020
DEFINITION  .
FEATURES             Location/Qualifiers
     Misc._feature   22..169
                     /label="LB"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    complement(92..116)
                     /label="LB T-DNA repeat"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    225..333
                     /label="OpattB"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    225..338
                     /label="MinSyn_068"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    256..279
                     /label="repeat seq"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    315..338
                     /label="repeat"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    343..346
                     /label="additional sites for pcr"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    347..455
                     /label="OpattB"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    378..401
                     /label="repeat seq"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    437..460
                     /label="repeat"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    460..508
                     /label="min 35S"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    516..536
                     /label="part of TMV 5'UTR"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    543..568
                     /label="part of TMV 5'UTR"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     5'_UTR          589..655
                     /label="Omega (TMV)"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    660..1823
                     /label="CrISY iridoid synthase >ENA JX974564 JX974564.1 Catharanthus roseus iridoid synthase mRNA, complete cds."
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    915..917
                     /label="0060a D87N mutation (GAT Asp to AAT Arg)"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     Terminator      1828..2031
                     /label="CaMV 35S terminator, pICH41414"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     Misc._feature   complement(2063..2190)
                     /label="RB short"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    complement(2077..2101)
                     /label="RB T-DNA repeat"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     conflict        2166..2184
                     /label="partial overdrive"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     Origin          complement(2214..3695)
                     /label="RK2"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     CDS             complement(2257..3405)
                     /label="Plasmid replication initiator protein TrfA, P07676 (TRFA_ECOLX)"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    complement(3726..4340)
                     /label="replicon of pMB1"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     rep_origin      complement(3726..4345)
                     /label="pBR322\(from\pUC18)"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     primer_bind     complement(3821..3840)
                     /label="pBR322ori-F"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     CDS             complement(4500..5360)
                     /label="AmpR"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     promoter        complement(5361..5465)
                     /label="AmpR promoter"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     rep_origin      complement(5552..6183)
                     /label="oriV"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
ORIGIN
        1 GCAAAGGAGA TCCTGATCTG ACTGATGGGC TGCCTGTATC GAGTGGTGAT TTTGTGCCGA
       61 GCTGCCGGTC GGGGAGCTGT TGGCTGGCTG GTGGCAGGAT ATATTGTGGT GTAAACAAAT
      121 TGACGCTTAG ACAACTTAAT AACACATTGC GGACGTTTTT AATGTACTGG GGTGGATGCA
      181 GTGGGCCCCA CTCTGTGAAG ACAATGCCGA ATTCGGATCC GGAGcggtgc gggtgccagg
      241 gcgtgccctt gggctccccg ggcgcgtact ccactccccg agccggtgcg ggtgccaggg
      301 cgtgcccttg ggctccccgg gcgcgtactc cactccccCC ATggtgcggt gcgggtgcca
      361 gggcgtgccc ttgggctccc cgggcgcgta ctccactccc cgagccggtg cgggtgccag
      421 ggcgtgccct tgggctcccc gggcgcgtac tccactcccc ttcgcaagac ccttcctcta
      481 tataaggaag ttcatttcat ttggagagga ctccggtatt tttacaacaa ttaccacaac
      541 aaaacaaaca acaaacaaca ttacaattta ctattctagt cgaaTACTGT ATTTTTACAA
      601 CAATTACCAA CAACAACAAA CAACAAACAA CATTACAATT ACTATTTACA ATTACAATGA
      661 GTTGGTGGTG GAAGAGGTCC ATTGGTGCTG GAAAGAACTT ACCAAACCAG AACAAAGAAA
      721 ATGGAGTTTG TAAAAGCTAC AAGAGTGTGG CACTAGTAGT AGGAGTCACC GGCATTGTTG
      781 GCAGTAGTTT AGCCGAAGTT CTAAAGCTAC CAGACACCCC TGGAGGTCCC TGGAAGGTAT
      841 ACGGCGTGGC AAGGCGCCCA TGCCCTGTAT GGCTAGCCAA AAAACCAGTT GAGTACATCC
      901 AATGTGATGT CTCAGATAAT CAAGAAACAA TTTCTAAATT ATCTCCACTT AAGGACATAA
      961 CTCATATATT CTATGTTTCT TGGATTGGAT CAGAAGATTG CCAAACAAAT GCTACAATGT
     1021 TCAAAAACAT CCTTAATTCA GTGATTCCTA ATGCTTCAAA TCTCCAACAT GTGTGTCTCC
     1081 AAACAGGAAT CAAACATTAT TTTGGTATTT TTGAAGAAGG TTCTAAAGTT GTACCACATG
     1141 ATTCGCCTTT TACCGAGGAT TTACCTAGGC TAAATGTCCC AAATTTCTAC CATGATCTTG
     1201 AAGATATACT GTATGAAGAA ACTGGCAAGA ACAATTTAAC ATGGTCTGTC CATAGACCAG
     1261 CTCTGGTTTT CGGGTTTTCC CCATGTAGTA TGATGAACAT TGTCAGTACA TTATGCGTTT
     1321 ACGCCACAAT TTGTAAACAT GAAAATAAGG CCTTGGTTTA TCCAGGTAGT AAAAATTCAT
     1381 GGAATTGTTA TGCTGATGCT GTTGATGCAG ATTTAGTAGC CGAGCACGAA ATTTGGGCAG
     1441 CAGTTGATCC TAAGGCTAAA AATCAAGTAT TGAATTGTAA CAATGGGGAT GTTTTTAAAT
     1501 GGAAACATAT TTGGAAGAAA TTAGCAGAGG AATTTGGGAT TGAAATGGTG GGATATGTTG
     1561 AAGGTAAAGA ACAAGTTAGT TTGGCTGAGT TAATGAAGGA TAAAGATCAA GTTTGGGATG
     1621 AAATTGTTAA GAAGAACAAT TTAGTACCTA CTAAATTGAA AGAAATTGCT GCCTTTTGGT
     1681 TTGCTGATAT TGCTTTTTGT TCTGAGAATT TGATTAGTAG TATGAACAAA AGTAAGGAAT
     1741 TAGGGTTCTT GGGTTTTAGG AATTCGATGA AATCGTTTGT TTCTTGTATT GATAAGATGA
     1801 GGGATTATAG GTTTATTCCT TAGGCTTCTC TAGCTAGAGT CGATCGACAA GCTCGAGTTT
     1861 CTCCATAATA ATGTGTGAGT AGTTCCCAGA TAAGGGAATT AGGGTTCCTA TAGGGTTTCG
     1921 CTCATGTGTT GAGCATATAA GAAACCCTTA GTATGTATTT GTATTTGTAA AATACTTCTA
     1981 TCAATAAAAT TTCTAATTCC TAAAACCAAA ATCCAGTACT AAAATCCAGA TCGCTGCAAT
     2041 TGTCTTCTGC ACGAAGTGGT TTAAACTATC AGTGTTTGAC AGGATATATT GGCGGGTAAA
     2101 CCTAAGAGAA AAGAGCGTTT ATTAGAATAA TCGGATATTT AAAAGGGCGT GAAAAGGTTT
     2161 ATCCGTTCGT CCATTTGTAT GTGCATGCCA ACCACAGGGT TCCCCAGATC AGGCGCTGGC
     2221 TGCTGAACCC CCAGCCGGAA CTGACCCCAC AAGGCCCTAG CGTTTGCAAT GCACCAGGTC
     2281 ATCATTGACC CAGGCGTGTT CCACCAGGCC GCTGCCTCGC AACTCTTCGC AGGCTTCGCC
     2341 GACCTGCTCG CGCCACTTCT TCACGCGGGT GGAATCCGAT CCGCACATGA GGCGGAAGGT
     2401 TTCCAGCTTG AGCGGGTACG GCTCCCGGTG CGAGCTGAAA TAGTCGAACA TCCGTCGGGC
     2461 CGTCGGCGAC AGCTTGCGGT ACTTCTCCCA TATGAATTTC GTGTAGTGGT CGCCAGCAAA
     2521 CAGCACGACG ATTTCCTCGT CGATCAGGAC CTGGCAACGG GACGTTTTCT TGCCACGGTC
     2581 CAGGACGCGG AAGCGGTGCA GCAGCGACAC CGATTCCAGG TGCCCAACGC GGTCGGACGT
     2641 GAAGCCCATC GCCGTCGCCT GTAGGCGCGA CAGGCATTCC TCGGCCTTCG TGTAATACCG
     2701 GCCATTGATC GACCAGCCCA GGTCCTGGCA AAGCTCGTAG AACGTGAAGG TGATCGGCTC
     2761 GCCGATAGGG GTGCGCTTCG CGTACTCCAA CACCTGCTGC CACACCAGTT CGTCATCGTC
     2821 GGCCCGCAGC TCGACGCCGG TGTAGGTGAT CTTCACGTCC TTGTTGACGT GGAAAATGAC
     2881 CTTGTTTTGC AGCGCCTCGC GCGGGATTTT CTTGTTGCGC GTGGTGAACA GGGCAGAGCG
     2941 GGCCGTGTCG TTTGGCATCG CTCGCATCGT GTCCGGCCAC GGCGCAATAT CGAACAAGGA
     3001 AAGCTGCATT TCCTTGATCT GCTGCTTCGT GTGTTTCAGC AACGCGGCCT GCTTGGCCTC
     3061 GCTGACCTGT TTTGCCAGGT CCTCGCCGGC GGTTTTTCGC TTCTTGGTCG TCATAGTTCC
     3121 TCGCGTGTCG ATGGTCATCG ACTTCGCCAA ACCTGCCGCC TCCTGTTCAA GACGACGCGA
     3181 ACGCTCCACG GCGGCCGATG GCGCGGGCAG GGCAGGGGGA GCCAGTTGCA CGCTGTCGCG
     3241 CTCGATCTTG GCCGTAGCTT GCTGGACCAT CGAGCCGACG GACTGGAAGG TTTCGCGGGG
     3301 CGCACGCATG ACGGTGCGGC TTGCGATGGT TTCGGCATCC TCGGCGGAAA ACCCCGCGTC
     3361 GATCAGTTCT TGCCTGTATG CCTTCCGGTC AAACGTCCGA TTCATTCACC CTCCTTGCGG
     3421 GATTGCCCCG ACTCACGCCG GGGCAATGTG CCCTTATTCC TGATTTGACC CGCCTGGTGC
     3481 CTTGGTGTCC AGATAATCCA CCTTATCGGC AATGAAGTCG GTCCCGTAGA CCGTCTGGCC
     3541 GTCCTTCTCG TACTTGGTAT TCCGAATCTT GCCCTGCACG AATACCAGCG ACCCCTTGCC
     3601 CAAATACTTG CCGTGGGCCT CGGCCTGAGA GCCAAAACAC TTGATGCGGA AGAAGTCGGT
     3661 GCGCTCCTGC TTGTCGCCGG CATCGTTGCG CCACATCTAG GATCTGCCAG GAACCGTAAA
     3721 AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT
     3781 CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC
     3841 CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC
     3901 GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT
     3961 TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC
     4021 CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG
     4081 CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA
     4141 GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC
     4201 GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA
     4261 ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA
     4321 GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC
     4381 TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA
     4441 AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAAACTTG GTCTGACAGT
     4501 TACCAATGCT TAATCAGTGA GGCACCTATC TCAGCGATCT GTCTATTTCG TTCATCCATA
     4561 GTTGCCTGAC TCCCCGTCGT GTAGATAACT ACGATACGGG AGGGCTTACC ATCTGGCCCC
     4621 AGTGCTGCAA TGATACCGCG AGAACCACGC TCACCGGCTC CAGATTTATC AGCAATAAAC
     4681 CAGCCAGCCG GAAGGGCCGA GCGCAGAAGT GGTCCTGCAA CTTTATCCGC CTCCATCCAG
     4741 TCTATTAATT GTTGCCGGGA AGCTAGAGTA AGTAGTTCGC CAGTTAATAG TTTGCGCAAC
     4801 GTTGTTGCCA TTGCTACAGG CATCGTGGTG TCACGCTCGT CGTTTGGTAT GGCTTCATTC
     4861 AGCTCCGGTT CCCAACGATC AAGGCGAGTT ACATGATCCC CCATGTTGTG CAAAAAAGCG
     4921 GTTAGCTCCT TCGGTCCTCC GATCGTTGTC AGAAGTAAGT TGGCCGCAGT GTTATCACTC
     4981 ATGGTTATGG CAGCACTGCA TAATTCTCTT ACTGTCATGC CATCCGTAAG ATGCTTTTCT
     5041 GTGACTGGTG AGTACTCAAC CAAGTCATTC TGAGAATAGT GTATGCGGCG ACCGAGTTGC
     5101 TCTTGCCCGG CGTCAATACG GGATAATACC GCGCCACATA GCAGAACTTT AAAAGTGCTC
     5161 ATCATTGGAA AACGTTCTTC GGGGCGAAAA CTCTCAAGGA TCTTACCGCT GTTGAGATCC
     5221 AGTTCGATGT AACCCACTCG TGCACCCAAC TGATCTTCAG CATCTTTTAC TTTCACCAGC
     5281 GTTTCTGGGT GAGCAAAAAC AGGAAGGCAA AATGCCGCAA AAAAGGGAAT AAGGGCGACA
     5341 CGGAAATGTT GAATACTCAT ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAAGGT
     5401 TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT
     5461 CCGCGCACGA ATTGGCCAGC GCTGCCATTT TTGGGGTGAG GCCGTTCGCG GCCGAGGGGC
     5521 GCAGCCCCTG GGGGGATGGG AGGCCCGCGT TAGCGGGCCG GGAGGGTTCG AGAAGGGGGG
     5581 GCACCCCCCT TCGGCGTGCG CGGTCACGCG CACAGGGCGC AGCCCTGGTT AAAAACAAGG
     5641 TTTATAAATA TTGGTTTAAA AGCAGGTTAA AAGACAGGTT AGCGGTGGCC GAAAAACGGG
     5701 CGGAAACCCT TGCAAATGCT GGATTTTCTG CCTGTGGACA GCCCCTCAAA TGTCAATAGG
     5761 TGCGCCCCTC ATCTGTCAGC ACTCTGCCCC TCAAGTGTCA AGGATCGCGC CCCTCATCTG
     5821 TCAGTAGTCG CGCCCCTCAA GTGTCAATAC CGCAGGGCAC TTATCCCCAG GCTTGTCCAC
     5881 ATCATCTGTG GGAAACTCGC GTAAAATCAG GCGTTTTCGC CGATTTGCGA GGCTGGCCAG
     5941 CTCCACGTCG CCGGCCGAAA TCGAGCCTGC CCCTCATCTG TCAACGCCGC GCCGGGTGAG
     6001 TCGGCCCCTC AAGTGTCAAC GTCCGCCCCT CATCTGTCAG TGAGGGCCAA GTTTTCCGCG
     6061 AGGTATCCAC AACGCCGGCG GCCGCGGTGT CTCGCACACG GCTTCGACGG CGTTTCTGGC
     6121 GCGTTTGCAG GGCCATAGAC GGCCGCCAGC CCAGCGGCGA GGGCAACCAG CCCGGTGAGC
     6181 GTC
//