LOCUS       pEPQD1CB0792_(P2_4X_opa 5545 bp ds-DNA     circular     14-OCT-2020
DEFINITION  .
FEATURES             Location/Qualifiers
     CDS             complement(292..1152)
                     /label="AmpR"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     rep_origin      complement(1344..1975)
                     /label="oriV"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    complement(1998..2148)
                     /label="LB"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    complement(2067..2091)
                     /label="LB T-DNA repeat"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    2200..2308
                     /label="OpattB"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    2200..2313
                     /label="MinSyn_068"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    2231..2254
                     /label="repeat seq"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    2290..2313
                     /label="repeat"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    2318..2321
                     /label="additional sites for pcr"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    2322..2430
                     /label="OpattB"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    2353..2376
                     /label="repeat seq"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    2412..2435
                     /label="repeat"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    2435..2483
                     /label="min 35S"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    2491..2511
                     /label="part of TMV 5'UTR"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    2518..2543
                     /label="part of TMV 5'UTR"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     5'_UTR          2564..2630
                     /label="Omega (TMV)"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    2635..3111
                     /label="MLPL1 from Nepeta mussinii (aka Nepeta racemosa) MT108267.1, with ISY will make cis-trans-nepetalactol ("cis-trans"-cyclase)"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    3112..3114
                     /label="Stop codon"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     Terminator      3119..3368
                     /label="Atu mas terminator from pICH77901"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    complement(3396..3521)
                     /label="RB"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    complement(3414..3438)
                     /label="RB T-DNA repeat"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     conflict        3503..3521
                     /label="partial overdrive"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     Origin          complement(3551..5032)
                     /label="RK2"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     CDS             complement(3594..4742)
                     /label="Plasmid replication initiator protein TrfA, P07676 (TRFA_ECOLX)"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     primer_bind     complement(5158..5177)
                     /label="pBR322ori-F"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
ORIGIN
        1 GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC
       61 TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA
      121 AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA
      181 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA
      241 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG
      301 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG
      361 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC
      421 AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC
      481 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA
      541 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC
      601 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG
      661 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC
      721 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT
      781 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG
      841 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC
      901 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG
      961 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT
     1021 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG
     1081 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG
     1141 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT
     1201 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC
     1261 GAATTGGCCA GCGCTGCCAT TTTTGGGGTG AGGCCGTTCG CGGCCGAGGG GCGCAGCCCC
     1321 TGGGGGGATG GGAGGCCCGC GTTAGCGGGC CGGGAGGGTT CGAGAAGGGG GGGCACCCCC
     1381 CTTCGGCGTG CGCGGTCACG CGCACAGGGC GCAGCCCTGG TTAAAAACAA GGTTTATAAA
     1441 TATTGGTTTA AAAGCAGGTT AAAAGACAGG TTAGCGGTGG CCGAAAAACG GGCGGAAACC
     1501 CTTGCAAATG CTGGATTTTC TGCCTGTGGA CAGCCCCTCA AATGTCAATA GGTGCGCCCC
     1561 TCATCTGTCA GCACTCTGCC CCTCAAGTGT CAAGGATCGC GCCCCTCATC TGTCAGTAGT
     1621 CGCGCCCCTC AAGTGTCAAT ACCGCAGGGC ACTTATCCCC AGGCTTGTCC ACATCATCTG
     1681 TGGGAAACTC GCGTAAAATC AGGCGTTTTC GCCGATTTGC GAGGCTGGCC AGCTCCACGT
     1741 CGCCGGCCGA AATCGAGCCT GCCCCTCATC TGTCAACGCC GCGCCGGGTG AGTCGGCCCC
     1801 TCAAGTGTCA ACGTCCGCCC CTCATCTGTC AGTGAGGGCC AAGTTTTCCG CGAGGTATCC
     1861 ACAACGCCGG CGGCCGCGGT GTCTCGCACA CGGCTTCGAC GGCGTTTCTG GCGCGTTTGC
     1921 AGGGCCATAG ACGGCCGCCA GCCCAGCGGC GAGGGCAACC AGCCCGGTGA GCGTCGCAAA
     1981 GGAGATCCTG ATCTGACTGA TGGGCTGCCT GTATCGAGTG GTGATTTTGT GCCGAGCTGC
     2041 CGGTCGGGGA GCTGTTGGCT GGCTGGTGGC AGGATATATT GTGGTGTAAA CAAATTGACG
     2101 CTTAGACAAC TTAATAACAC ATTGCGGACG TTTTTAATGT ACTGGGGTGG ATGCAGTGGG
     2161 CCCCACTCTG TGAAGACAAG CAAGAATTCA AGCTTGGAGc ggtgcgggtg ccagggcgtg
     2221 cccttgggct ccccgggcgc gtactccact ccccgagccg gtgcgggtgc cagggcgtgc
     2281 ccttgggctc cccgggcgcg tactccactc cccCCATggt gcggtgcggg tgccagggcg
     2341 tgcccttggg ctccccgggc gcgtactcca ctccccgagc cggtgcgggt gccagggcgt
     2401 gcccttgggc tccccgggcg cgtactccac tccccttcgc aagacccttc ctctatataa
     2461 ggaagttcat ttcatttgga gaggactccg gtatttttac aacaattacc acaacaaaac
     2521 aaacaacaaa caacattaca atttactatt ctagtcgaaT ACTGTATTTT TACAACAATT
     2581 ACCAACAACA ACAAACAACA AACAACATTA CAATTACTAT TTACAATTAC AATGGCTTCG
     2641 AAGCTTGAAA TAGAAATTGA GTTGAAAAGT GATGTAGAAA AGATGTGGAA ACACTTCAAA
     2701 GAATTCACCA AATTATTCCC CAAGGCTTGT CCTCATCTTT ACGAGAAGAT TGATGTGATC
     2761 GAGGGAGATG GGATATCAGT TGGAACAATC TTTGTCAGTA CCCTGAAACC AACAGAGTTG
     2821 AACCCCGTGG TTATGGTGAC GAAGGAGAAG ATTGATTTTT TAGATGATGA GAACAAAATG
     2881 TTGAGGTATA GTTACATGGA AGGGGAGATA TTAAAGAATT ACAAGAATTT GAGGGGCACA
     2941 GTGCATATGA GCAGCAGCAA AAGTGGTGGA ACTATATTCA AATATTCAGG AGAATTCGAA
     3001 AAGGCAAATG AACAAGTCCC TGATCCTGTT TTCTTCAAAG ATTTTATGGT CATAGTTTTC
     3061 CAAGGTTTGG ATGATTATAT TCTCAAGGGC ATGAACCATA CATGTCAAAA TTAAGCTTGG
     3121 ACTCCCATGT TGGCAAAGGC AACCAAACAA ACAATGAATG ATCCGCTCCT GCATATGGGG
     3181 CGGTTTGAGT ATTTCAACTG CCATTTGGGC TGAATTGTAG ACATGCTCCT GTCAGAAATT
     3241 CCGTGATCTT ACTCAATATT CAGTAATCTC GGCCAATATC CTAAATGTGC GTGGCTTTAT
     3301 CTGTCTTTGT ATTGTTTCAT CAATTCATGT AACGTTTGCT TTTCTTATGA ATTTTCAAAT
     3361 AAATTATCCG CTACTATTGT CTTCTGCACG AAGTGGTTTA AACTATCAGT GTTTGACAGG
     3421 ATATATTGGC GGGTAAACCT AAGAGAAAAG AGCGTTTATT AGAATAATCG GATATTTAAA
     3481 AGGGCGTGAA AAGGTTTATC CGTTCGTCCA TTTGTATGTG CATGCCAACC ACAGGGTTCC
     3541 CCAGATCAGG CGCTGGCTGC TGAACCCCCA GCCGGAACTG ACCCCACAAG GCCCTAGCGT
     3601 TTGCAATGCA CCAGGTCATC ATTGACCCAG GCGTGTTCCA CCAGGCCGCT GCCTCGCAAC
     3661 TCTTCGCAGG CTTCGCCGAC CTGCTCGCGC CACTTCTTCA CGCGGGTGGA ATCCGATCCG
     3721 CACATGAGGC GGAAGGTTTC CAGCTTGAGC GGGTACGGCT CCCGGTGCGA GCTGAAATAG
     3781 TCGAACATCC GTCGGGCCGT CGGCGACAGC TTGCGGTACT TCTCCCATAT GAATTTCGTG
     3841 TAGTGGTCGC CAGCAAACAG CACGACGATT TCCTCGTCGA TCAGGACCTG GCAACGGGAC
     3901 GTTTTCTTGC CACGGTCCAG GACGCGGAAG CGGTGCAGCA GCGACACCGA TTCCAGGTGC
     3961 CCAACGCGGT CGGACGTGAA GCCCATCGCC GTCGCCTGTA GGCGCGACAG GCATTCCTCG
     4021 GCCTTCGTGT AATACCGGCC ATTGATCGAC CAGCCCAGGT CCTGGCAAAG CTCGTAGAAC
     4081 GTGAAGGTGA TCGGCTCGCC GATAGGGGTG CGCTTCGCGT ACTCCAACAC CTGCTGCCAC
     4141 ACCAGTTCGT CATCGTCGGC CCGCAGCTCG ACGCCGGTGT AGGTGATCTT CACGTCCTTG
     4201 TTGACGTGGA AAATGACCTT GTTTTGCAGC GCCTCGCGCG GGATTTTCTT GTTGCGCGTG
     4261 GTGAACAGGG CAGAGCGGGC CGTGTCGTTT GGCATCGCTC GCATCGTGTC CGGCCACGGC
     4321 GCAATATCGA ACAAGGAAAG CTGCATTTCC TTGATCTGCT GCTTCGTGTG TTTCAGCAAC
     4381 GCGGCCTGCT TGGCCTCGCT GACCTGTTTT GCCAGGTCCT CGCCGGCGGT TTTTCGCTTC
     4441 TTGGTCGTCA TAGTTCCTCG CGTGTCGATG GTCATCGACT TCGCCAAACC TGCCGCCTCC
     4501 TGTTCAAGAC GACGCGAACG CTCCACGGCG GCCGATGGCG CGGGCAGGGC AGGGGGAGCC
     4561 AGTTGCACGC TGTCGCGCTC GATCTTGGCC GTAGCTTGCT GGACCATCGA GCCGACGGAC
     4621 TGGAAGGTTT CGCGGGGCGC ACGCATGACG GTGCGGCTTG CGATGGTTTC GGCATCCTCG
     4681 GCGGAAAACC CCGCGTCGAT CAGTTCTTGC CTGTATGCCT TCCGGTCAAA CGTCCGATTC
     4741 ATTCACCCTC CTTGCGGGAT TGCCCCGACT CACGCCGGGG CAATGTGCCC TTATTCCTGA
     4801 TTTGACCCGC CTGGTGCCTT GGTGTCCAGA TAATCCACCT TATCGGCAAT GAAGTCGGTC
     4861 CCGTAGACCG TCTGGCCGTC CTTCTCGTAC TTGGTATTCC GAATCTTGCC CTGCACGAAT
     4921 ACCAGCGACC CCTTGCCCAA ATACTTGCCG TGGGCCTCGG CCTGAGAGCC AAAACACTTG
     4981 ATGCGGAAGA AGTCGGTGCG CTCCTGCTTG TCGCCGGCAT CGTTGCGCCA CATCTAGGAT
     5041 CTGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG
     5101 ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA
     5161 GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC
     5221 TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC
     5281 GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC
     5341 CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG
     5401 TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT
     5461 ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGGA
     5521 CAGTATTTGG TATCTGCGCT CTGCT
//