LOCUS       pEPQD1CB0798_(P5_4X_opa 6564 bp ds-DNA     circular     14-OCT-2020
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    complement(23..173)
                     /label="LB"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    complement(92..116)
                     /label="LB T-DNA repeat"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    225..333
                     /label="OpattB"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    225..338
                     /label="MinSyn_068"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    256..279
                     /label="repeat seq"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    315..338
                     /label="repeat"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    343..346
                     /label="additional sites for pcr"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    347..455
                     /label="OpattB"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    378..401
                     /label="repeat seq"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    437..460
                     /label="repeat"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    460..508
                     /label="min 35S"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    516..536
                     /label="part of TMV 5'UTR"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    543..568
                     /label="part of TMV 5'UTR"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     5'_UTR          589..655
                     /label="Omega (TMV)"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    660..2204
                     /label="CrIO (contains T359S mutation, domesticated, 1 BpiI site and 1 BsmBI site removed) >ENA AHK60833 AHK60833.1 Catharanthus roseus (Madagascar periwinkle) iridoid oxidase "
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    1731..1733
                     /label="ACC threo to AGC ser"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    1732..1732
                     /label="SNP here? Sequencing says G. YES it is a G"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    1773..1775
                     /label="GAG glu to GAA glu to remove BsmBI"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_feature    1827..1829
                     /label="GAA glu to GAG glu"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     Terminator      2209..2412
                     /label="CaMV 35S terminator, pICH41414"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    complement(2423..2428)
                     /label="BpiI"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    complement(2440..2565)
                     /label="RB"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    complement(2458..2482)
                     /label="RB T-DNA repeat"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     conflict        2547..2565
                     /label="partial overdrive"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     Origin          complement(2595..4076)
                     /label="RK2"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     CDS             complement(2638..3786)
                     /label="Plasmid replication initiator protein TrfA, P07676 (TRFA_ECOLX)"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     primer_bind     complement(4202..4221)
                     /label="pBR322ori-F"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     CDS             complement(4881..5741)
                     /label="AmpR"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     rep_origin      complement(5933..6564)
                     /label="oriV"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
ORIGIN
        1 GCAAAGGAGA TCCTGATCTG ACTGATGGGC TGCCTGTATC GAGTGGTGAT TTTGTGCCGA
       61 GCTGCCGGTC GGGGAGCTGT TGGCTGGCTG GTGGCAGGAT ATATTGTGGT GTAAACAAAT
      121 TGACGCTTAG ACAACTTAAT AACACATTGC GGACGTTTTT AATGTACTGG GGTGGATGCA
      181 GTGGGCCCCA CTCTGTGAAG ACAACAGAGA ATTCGCATGC GGAGcggtgc gggtgccagg
      241 gcgtgccctt gggctccccg ggcgcgtact ccactccccg agccggtgcg ggtgccaggg
      301 cgtgcccttg ggctccccgg gcgcgtactc cactccccCC ATggtgcggt gcgggtgcca
      361 gggcgtgccc ttgggctccc cgggcgcgta ctccactccc cgagccggtg cgggtgccag
      421 ggcgtgccct tgggctcccc gggcgcgtac tccactcccc ttcgcaagac ccttcctcta
      481 tataaggaag ttcatttcat ttggagagga ctccggtatt tttacaacaa ttaccacaac
      541 aaaacaaaca acaaacaaca ttacaattta ctattctagt cgaaTACTGT ATTTTTACAA
      601 CAATTACCAA CAACAACAAA CAACAAACAA CATTACAATT ACTATTTACA ATTACAATGG
      661 CGACCATCAC TTTCGATTCA CTAAACCCGG TGACCGTCGC CATCTCAGCC GGTTTTCTTC
      721 TCCTCCTTAT CATCTTTGTG AAATCAAGAA CTGGTTCATC CAAAAGAAAA CCGCCGGGTC
      781 CTCCAGGATG GCCGATTTTC GGCAATATGT TCGACCTCGG AGATTTACCT CACCAAACGC
      841 TGTACAAGCT CAAGTCCAAA TACGGGCCGA TCGTATGGCT CCAGCTCGGC TCAATTAATA
      901 CTATGGTTGT TCAAAATGCA GTTTCCGCCG CCGAGCTTTT TAAGAAACAC GATGTCCCCT
      961 TTTGTGACCG AAAAGTCCCT GATACTCTCA CTGCCTTCAA TTTCAACCAG GGCTCCCTGG
     1021 GTATGAATAC TTACGGCGGA CATTGGCGAG TGCTTAGACG CCTCTGTTCC ATGGAGTTTT
     1081 TGGTAAATAA ACGTATGAAC GAAACTACAG ATCTAAGAAG GAGAATTGAG GACAATATGG
     1141 TCCGTTGGAT TGAAGAAGAT TCATTGGCAT CTAAAGCACA AGGAGGAACC GGAGCGGTTC
     1201 AATTGAGCAG GTTTCTTTTT CTAATGGCGT TTAATTTGGT GGGTAACCTT ATGTTATCGA
     1261 GAGATTTAAT GGATAATAAG GATCCAGAAG GAAGGGAATT TTTTGATTGT ATGAATGAGA
     1321 TATTGGAATT GGCTGGTACT CCTAATATTG CTGATTTTTT GCCTTTGTTG AAGAAATTGG
     1381 ATCCACTTGG AATGAAGAAA AGGATGGTTG ATAATATGTC TAGAACTATG AAAATTTCTT
     1441 CAAAGTTTGT TCAAGAAAGA CTTGATAATA GAAAAGCTGG AAAGATTAAT GAAAAGAAAG
     1501 ATTTCTTGGA TGTTATGCTT GAATACCAAG GTGATGGTAA AGATGGTCCC GATAAATTCA
     1561 CTGAGCAACA TGTCAACATT GTCATCATGG AAATGTTTTT TGCAGGATCA GAGACAACAA
     1621 GTATCAGCAT TGAATGGGGA TTCACAGAAC TTCTCCGAAA CCCACACGCA TTCAAGAAAG
     1681 TAAGGGAAGA GATCGACAGA GTAGTGGGAG TAAACAGAAT GGTCGAAGAG AGCGACATGG
     1741 AGAATTTGCC GTATTTGCAA GCAGTTGTAA AGGAAACGCT TAGATTGCAT CCGGCACTTC
     1801 CAATGCTTCT ACCAAGAAAC ACAATGGAGG ACACTGAATA CATGGGATAC CTCATTCCTA
     1861 AAGGCACACA AGTTTTCGTG AATGCATGGG CAATCGGAAG GGATCCTGAG TACTGGCAGG
     1921 ACCCTTTGAG TTTCAAACCA GAAAGGTTTA TCAATTCCAG TGTTGAATAT AAAGGACAAC
     1981 ATTTTGAATT GATTCCTTTT GGTTCTGGAA GAAGAATTTG TGTGGGATTT CCTTTGGCTC
     2041 ATAGAGTTGT TCATCTCACT TTGGCTACAT TAGTCCAGGC TTTTGATTGG GATCTTGGTG
     2101 CTGGCGTTAA ACCACAAGAT ATTGATTTGG AAGAGAGATT GGGATTGACT CTGAGGAAGA
     2161 AGAATCCATT GAATGTCATC CCTAAGAAGA GAGTTCATAT CTAAGCTTCT CTAGCTAGAG
     2221 TCGATCGACA AGCTCGAGTT TCTCCATAAT AATGTGTGAG TAGTTCCCAG ATAAGGGAAT
     2281 TAGGGTTCCT ATAGGGTTTC GCTCATGTGT TGAGCATATA AGAAACCCTT AGTATGTATT
     2341 TGTATTTGTA AAATACTTCT ATCAATAAAA TTTCTAATTC CTAAAACCAA AATCCAGTAC
     2401 TAAAATCCAG ATCGCTTGTG TTGTCTTCTG CACGAAGTGG TTTAAACTAT CAGTGTTTGA
     2461 CAGGATATAT TGGCGGGTAA ACCTAAGAGA AAAGAGCGTT TATTAGAATA ATCGGATATT
     2521 TAAAAGGGCG TGAAAAGGTT TATCCGTTCG TCCATTTGTA TGTGCATGCC AACCACAGGG
     2581 TTCCCCAGAT CAGGCGCTGG CTGCTGAACC CCCAGCCGGA ACTGACCCCA CAAGGCCCTA
     2641 GCGTTTGCAA TGCACCAGGT CATCATTGAC CCAGGCGTGT TCCACCAGGC CGCTGCCTCG
     2701 CAACTCTTCG CAGGCTTCGC CGACCTGCTC GCGCCACTTC TTCACGCGGG TGGAATCCGA
     2761 TCCGCACATG AGGCGGAAGG TTTCCAGCTT GAGCGGGTAC GGCTCCCGGT GCGAGCTGAA
     2821 ATAGTCGAAC ATCCGTCGGG CCGTCGGCGA CAGCTTGCGG TACTTCTCCC ATATGAATTT
     2881 CGTGTAGTGG TCGCCAGCAA ACAGCACGAC GATTTCCTCG TCGATCAGGA CCTGGCAACG
     2941 GGACGTTTTC TTGCCACGGT CCAGGACGCG GAAGCGGTGC AGCAGCGACA CCGATTCCAG
     3001 GTGCCCAACG CGGTCGGACG TGAAGCCCAT CGCCGTCGCC TGTAGGCGCG ACAGGCATTC
     3061 CTCGGCCTTC GTGTAATACC GGCCATTGAT CGACCAGCCC AGGTCCTGGC AAAGCTCGTA
     3121 GAACGTGAAG GTGATCGGCT CGCCGATAGG GGTGCGCTTC GCGTACTCCA ACACCTGCTG
     3181 CCACACCAGT TCGTCATCGT CGGCCCGCAG CTCGACGCCG GTGTAGGTGA TCTTCACGTC
     3241 CTTGTTGACG TGGAAAATGA CCTTGTTTTG CAGCGCCTCG CGCGGGATTT TCTTGTTGCG
     3301 CGTGGTGAAC AGGGCAGAGC GGGCCGTGTC GTTTGGCATC GCTCGCATCG TGTCCGGCCA
     3361 CGGCGCAATA TCGAACAAGG AAAGCTGCAT TTCCTTGATC TGCTGCTTCG TGTGTTTCAG
     3421 CAACGCGGCC TGCTTGGCCT CGCTGACCTG TTTTGCCAGG TCCTCGCCGG CGGTTTTTCG
     3481 CTTCTTGGTC GTCATAGTTC CTCGCGTGTC GATGGTCATC GACTTCGCCA AACCTGCCGC
     3541 CTCCTGTTCA AGACGACGCG AACGCTCCAC GGCGGCCGAT GGCGCGGGCA GGGCAGGGGG
     3601 AGCCAGTTGC ACGCTGTCGC GCTCGATCTT GGCCGTAGCT TGCTGGACCA TCGAGCCGAC
     3661 GGACTGGAAG GTTTCGCGGG GCGCACGCAT GACGGTGCGG CTTGCGATGG TTTCGGCATC
     3721 CTCGGCGGAA AACCCCGCGT CGATCAGTTC TTGCCTGTAT GCCTTCCGGT CAAACGTCCG
     3781 ATTCATTCAC CCTCCTTGCG GGATTGCCCC GACTCACGCC GGGGCAATGT GCCCTTATTC
     3841 CTGATTTGAC CCGCCTGGTG CCTTGGTGTC CAGATAATCC ACCTTATCGG CAATGAAGTC
     3901 GGTCCCGTAG ACCGTCTGGC CGTCCTTCTC GTACTTGGTA TTCCGAATCT TGCCCTGCAC
     3961 GAATACCAGC GACCCCTTGC CCAAATACTT GCCGTGGGCC TCGGCCTGAG AGCCAAAACA
     4021 CTTGATGCGG AAGAAGTCGG TGCGCTCCTG CTTGTCGCCG GCATCGTTGC GCCACATCTA
     4081 GGATCTGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC
     4141 CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA
     4201 TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG
     4261 CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC
     4321 TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC
     4381 GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC
     4441 CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG
     4501 AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA
     4561 AGGACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT
     4621 AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG
     4681 CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT
     4741 GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG
     4801 ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT
     4861 GAGTAAACTT GGTCTGACAG TTACCAATGC TTAATCAGTG AGGCACCTAT CTCAGCGATC
     4921 TGTCTATTTC GTTCATCCAT AGTTGCCTGA CTCCCCGTCG TGTAGATAAC TACGATACGG
     4981 GAGGGCTTAC CATCTGGCCC CAGTGCTGCA ATGATACCGC GAGAACCACG CTCACCGGCT
     5041 CCAGATTTAT CAGCAATAAA CCAGCCAGCC GGAAGGGCCG AGCGCAGAAG TGGTCCTGCA
     5101 ACTTTATCCG CCTCCATCCA GTCTATTAAT TGTTGCCGGG AAGCTAGAGT AAGTAGTTCG
     5161 CCAGTTAATA GTTTGCGCAA CGTTGTTGCC ATTGCTACAG GCATCGTGGT GTCACGCTCG
     5221 TCGTTTGGTA TGGCTTCATT CAGCTCCGGT TCCCAACGAT CAAGGCGAGT TACATGATCC
     5281 CCCATGTTGT GCAAAAAAGC GGTTAGCTCC TTCGGTCCTC CGATCGTTGT CAGAAGTAAG
     5341 TTGGCCGCAG TGTTATCACT CATGGTTATG GCAGCACTGC ATAATTCTCT TACTGTCATG
     5401 CCATCCGTAA GATGCTTTTC TGTGACTGGT GAGTACTCAA CCAAGTCATT CTGAGAATAG
     5461 TGTATGCGGC GACCGAGTTG CTCTTGCCCG GCGTCAATAC GGGATAATAC CGCGCCACAT
     5521 AGCAGAACTT TAAAAGTGCT CATCATTGGA AAACGTTCTT CGGGGCGAAA ACTCTCAAGG
     5581 ATCTTACCGC TGTTGAGATC CAGTTCGATG TAACCCACTC GTGCACCCAA CTGATCTTCA
     5641 GCATCTTTTA CTTTCACCAG CGTTTCTGGG TGAGCAAAAA CAGGAAGGCA AAATGCCGCA
     5701 AAAAAGGGAA TAAGGGCGAC ACGGAAATGT TGAATACTCA TACTCTTCCT TTTTCAATAT
     5761 TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG
     5821 AAAAATAAAC AAATAGGGGT TCCGCGCACG AATTGGCCAG CGCTGCCATT TTTGGGGTGA
     5881 GGCCGTTCGC GGCCGAGGGG CGCAGCCCCT GGGGGGATGG GAGGCCCGCG TTAGCGGGCC
     5941 GGGAGGGTTC GAGAAGGGGG GGCACCCCCC TTCGGCGTGC GCGGTCACGC GCACAGGGCG
     6001 CAGCCCTGGT TAAAAACAAG GTTTATAAAT ATTGGTTTAA AAGCAGGTTA AAAGACAGGT
     6061 TAGCGGTGGC CGAAAAACGG GCGGAAACCC TTGCAAATGC TGGATTTTCT GCCTGTGGAC
     6121 AGCCCCTCAA ATGTCAATAG GTGCGCCCCT CATCTGTCAG CACTCTGCCC CTCAAGTGTC
     6181 AAGGATCGCG CCCCTCATCT GTCAGTAGTC GCGCCCCTCA AGTGTCAATA CCGCAGGGCA
     6241 CTTATCCCCA GGCTTGTCCA CATCATCTGT GGGAAACTCG CGTAAAATCA GGCGTTTTCG
     6301 CCGATTTGCG AGGCTGGCCA GCTCCACGTC GCCGGCCGAA ATCGAGCCTG CCCCTCATCT
     6361 GTCAACGCCG CGCCGGGTGA GTCGGCCCCT CAAGTGTCAA CGTCCGCCCC TCATCTGTCA
     6421 GTGAGGGCCA AGTTTTCCGC GAGGTATCCA CAACGCCGGC GGCCGCGGTG TCTCGCACAC
     6481 GGCTTCGACG GCGTTTCTGG CGCGTTTGCA GGGCCATAGA CGGCCGCCAG CCCAGCGGCG
     6541 AGGGCAACCA GCCCGGTGAG CGTC
//