LOCUS       pEPQD1CB0861_(P6_4X_opa 6524 bp ds-DNA     circular     14-DEC-2020
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    complement(47..197)
                     /label="LB"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    complement(116..140)
                     /label="LB T-DNA repeat"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    249..357
                     /label="OpattB"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    249..362
                     /label="MinSyn_068"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    280..303
                     /label="repeat seq"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    339..362
                     /label="repeat"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    367..370
                     /label="additional sites for pcr"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    371..479
                     /label="OpattB"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    402..425
                     /label="repeat seq"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    461..484
                     /label="repeat"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    484..532
                     /label="min 35S"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    540..560
                     /label="part of TMV 5'UTR"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    567..592
                     /label="part of TMV 5'UTR"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    613..679
                     /label="TMV omega"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    684..2126
                     /label="Cr7-DLGT (aka CrUGT8) (domesticated, 1 BsaI site removed) UGT709C2 AB733667.1 >ENA BAO01109 BAO01109.1 Catharanthus roseus (Madagascar periwinkle) UDP-glucose iridoid glucosyltransferase"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     Terminator      2134..2396
                     /label="Atu nos terminator, pICH41421"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    complement(2424..2549)
                     /label="RB"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    complement(2442..2466)
                     /label="RB T-DNA repeat"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     conflict        2531..2549
                     /label="partial overdrive"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     Origin          complement(2579..4060)
                     /label="RK2"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     CDS             complement(2622..3770)
                     /label="Plasmid replication initiator protein TrfA, P07676 (TRFA_ECOLX)"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     primer_bind     complement(4186..4205)
                     /label="pBR322ori-F"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     CDS             complement(4865..5725)
                     /label="AmpR"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     rep_origin      complement(join(5917..6524,1..24))
                     /label="oriV"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
ORIGIN
        1 AGGGCAACCA GCCCGGTGAG CGTCGCAAAG GAGATCCTGA TCTGACTGAT GGGCTGCCTG
       61 TATCGAGTGG TGATTTTGTG CCGAGCTGCC GGTCGGGGAG CTGTTGGCTG GCTGGTGGCA
      121 GGATATATTG TGGTGTAAAC AAATTGACGC TTAGACAACT TAATAACACA TTGCGGACGT
      181 TTTTAATGTA CTGGGGTGGA TGCAGTGGGC CCCACTCTGT GAAGACAATG TGGAATTCCT
      241 CGAGGGAGcg gtgcgggtgc cagggcgtgc ccttgggctc cccgggcgcg tactccactc
      301 cccgagccgg tgcgggtgcc agggcgtgcc cttgggctcc ccgggcgcgt actccactcc
      361 ccCCATggtg cggtgcgggt gccagggcgt gcccttgggc tccccgggcg cgtactccac
      421 tccccgagcc ggtgcgggtg ccagggcgtg cccttgggct ccccgggcgc gtactccact
      481 ccccttcgca agacccttcc tctatataag gaagttcatt tcatttggag aggactccgg
      541 tatttttaca acaattacca caacaaaaca aacaacaaac aacattacaa tttactattc
      601 tagtcgaaTA CTGTATTTTT ACAACAATTA CCAACAACAA CAAACAACAA ACAACATTAC
      661 AATTACTATT TACAATTACA ATGGGTTCTC AAGAAACAAA TTTGGCTCCT CATGTTCTCA
      721 TATTCCCTTT GCCAATTCAA GGCCATGTGA ACTCCATGCT CAGACTAGCA GAACTTCTCT
      781 GTCTAGCTGA ATTAGATATC ACTTTTATTG TTTCAGAGTT CAGCCACAGT CGCTTAATTA
      841 AGCATACAAA TGTTGCCTCC CGTTTTGCCC GTTACCCAGG ATTTCAGTTC CAGCCCATAT
      901 CCGATGGCCT TCCCGATGAT CACCCCAGAG CAGGCGAAAG AGTCATGGAT ATTTTGCCTT
      961 CAACTAAAAA TGTAACGGGA CCCCTGTTTA AGCAGATGAT GGTTGAGAAT AAGTGCTTTT
     1021 CTTCTGCTAC TCGAAGGCCG ATTACTTGCA TAATCGCTGA TGGGGTTTTG AGCTTTGCTG
     1081 GGGATTTCGC TCAAGAAAAA GGAATCCCTC TTATTTACTT CAGAACTGTT TCTGCTTGTT
     1141 CTTTCTGGGC TTGTTTTTGT ATGCCTGAAC TAATTGAATC TGGTGACATT CCGATTAAAG
     1201 GAAATGGTAT GGATCTGATA GTGAAAAGCG TGCCGGGGAT GGAAACTTTT CTCAGGCGAC
     1261 GAGATCTGCC GGGGTTTTGC CGAGTCAATG ACATTAATGA ACCGAAACTC CAGATCCTGA
     1321 AAACAGAGAC AAGACAGACA ACGAGAGCCC AAGCGGCTAT TTTGAACACT TTTGAGGATT
     1381 TGGAAGGACC AATTCTATCT CAAATTCGCA AACACATGCC AAGACTTTTC ACAATTGGAC
     1441 CAAGTCATTC CCACCTAACA TCTAGACTTG AAACCAAGAA TATCAAGACT TTAATTTCCT
     1501 CCGGTAGTTT CTGGGAAGAA GATCGAAGCT GTGTGGATTG GCTTGATGCA CAGCCACCAA
     1561 GATCTGTTTT ATATGTAAGT TTTGGAAGTA TTACAGTTGT AACAAGAGAT CAACTCCTGG
     1621 AATTCTGGTA TGGTCTTGTT AATAGTGGAC AACGGTTCTT GTGGGTAATG AGGCCAGATT
     1681 CAATTATGGG CAAAGATGGG CAAAGCCAGA TTCCTGCAGA TCTTGAAGAG GGCACAAAAG
     1741 CCAGAGGTTA TATGGTGGGA TGGGCACCTC AAGAAGAGGT CTTGAATCAC CCTGCCATTG
     1801 GAGGATTTCT GACTCACAGT GGGTGGAATT CAACTTTAGA GAGTATTGTG GCTGGTGTCC
     1861 CAATGATATG TTGGCCATAC TTTGCGGACC AAATGATAAA CAGCAGATTT GTGAGTGAAA
     1921 TTTGGAAGAT TGGATTGGAT ATGAAAGATA CATGTGATAG AGAGACAATT GTGAAGATGG
     1981 TTAGGGAGTT GATGGAGATT AGGAAAGATG AGTTCTTGCA AAGGGCTGAT CATATGGCTA
     2041 AATTGGCAAA AGAGGCTGTT AGTGAAGGCG GATCCTCATA TTCTAACTTG GATGGCCTCG
     2101 TTGATTACAT AAAATCACTG ATTATTTGAG CTTGTCAAGC AGATCGTTCA AACATTTGGC
     2161 AATAAAGTTT CTTAAGATTG AATCCTGTTG CCGGTCTTGC GATGATTATC ATATAATTTC
     2221 TGTTGAATTA CGTTAAGCAT GTAATAATTA ACATGTAATG CATGACGTTA TTTATGAGAT
     2281 GGGTTTTTAT GATTAGAGTC CCGCAATTAT ACATTTAATA CGCGATAGAA AACAAAATAT
     2341 AGCGCGCAAA CTAGGATAAA TTATCGCGCG CGGTGTCATC TATGTTACTA GATCGACGCT
     2401 GAGCTTGTCT TCTGCACGAA GTGGTTTAAA CTATCAGTGT TTGACAGGAT ATATTGGCGG
     2461 GTAAACCTAA GAGAAAAGAG CGTTTATTAG AATAATCGGA TATTTAAAAG GGCGTGAAAA
     2521 GGTTTATCCG TTCGTCCATT TGTATGTGCA TGCCAACCAC AGGGTTCCCC AGATCAGGCG
     2581 CTGGCTGCTG AACCCCCAGC CGGAACTGAC CCCACAAGGC CCTAGCGTTT GCAATGCACC
     2641 AGGTCATCAT TGACCCAGGC GTGTTCCACC AGGCCGCTGC CTCGCAACTC TTCGCAGGCT
     2701 TCGCCGACCT GCTCGCGCCA CTTCTTCACG CGGGTGGAAT CCGATCCGCA CATGAGGCGG
     2761 AAGGTTTCCA GCTTGAGCGG GTACGGCTCC CGGTGCGAGC TGAAATAGTC GAACATCCGT
     2821 CGGGCCGTCG GCGACAGCTT GCGGTACTTC TCCCATATGA ATTTCGTGTA GTGGTCGCCA
     2881 GCAAACAGCA CGACGATTTC CTCGTCGATC AGGACCTGGC AACGGGACGT TTTCTTGCCA
     2941 CGGTCCAGGA CGCGGAAGCG GTGCAGCAGC GACACCGATT CCAGGTGCCC AACGCGGTCG
     3001 GACGTGAAGC CCATCGCCGT CGCCTGTAGG CGCGACAGGC ATTCCTCGGC CTTCGTGTAA
     3061 TACCGGCCAT TGATCGACCA GCCCAGGTCC TGGCAAAGCT CGTAGAACGT GAAGGTGATC
     3121 GGCTCGCCGA TAGGGGTGCG CTTCGCGTAC TCCAACACCT GCTGCCACAC CAGTTCGTCA
     3181 TCGTCGGCCC GCAGCTCGAC GCCGGTGTAG GTGATCTTCA CGTCCTTGTT GACGTGGAAA
     3241 ATGACCTTGT TTTGCAGCGC CTCGCGCGGG ATTTTCTTGT TGCGCGTGGT GAACAGGGCA
     3301 GAGCGGGCCG TGTCGTTTGG CATCGCTCGC ATCGTGTCCG GCCACGGCGC AATATCGAAC
     3361 AAGGAAAGCT GCATTTCCTT GATCTGCTGC TTCGTGTGTT TCAGCAACGC GGCCTGCTTG
     3421 GCCTCGCTGA CCTGTTTTGC CAGGTCCTCG CCGGCGGTTT TTCGCTTCTT GGTCGTCATA
     3481 GTTCCTCGCG TGTCGATGGT CATCGACTTC GCCAAACCTG CCGCCTCCTG TTCAAGACGA
     3541 CGCGAACGCT CCACGGCGGC CGATGGCGCG GGCAGGGCAG GGGGAGCCAG TTGCACGCTG
     3601 TCGCGCTCGA TCTTGGCCGT AGCTTGCTGG ACCATCGAGC CGACGGACTG GAAGGTTTCG
     3661 CGGGGCGCAC GCATGACGGT GCGGCTTGCG ATGGTTTCGG CATCCTCGGC GGAAAACCCC
     3721 GCGTCGATCA GTTCTTGCCT GTATGCCTTC CGGTCAAACG TCCGATTCAT TCACCCTCCT
     3781 TGCGGGATTG CCCCGACTCA CGCCGGGGCA ATGTGCCCTT ATTCCTGATT TGACCCGCCT
     3841 GGTGCCTTGG TGTCCAGATA ATCCACCTTA TCGGCAATGA AGTCGGTCCC GTAGACCGTC
     3901 TGGCCGTCCT TCTCGTACTT GGTATTCCGA ATCTTGCCCT GCACGAATAC CAGCGACCCC
     3961 TTGCCCAAAT ACTTGCCGTG GGCCTCGGCC TGAGAGCCAA AACACTTGAT GCGGAAGAAG
     4021 TCGGTGCGCT CCTGCTTGTC GCCGGCATCG TTGCGCCACA TCTAGGATCT GCCAGGAACC
     4081 GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA
     4141 AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT
     4201 TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC
     4261 TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC
     4321 TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC
     4381 CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT
     4441 TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG
     4501 CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGGACA GTATTTGGTA
     4561 TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA
     4621 AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA
     4681 AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG
     4741 AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC
     4801 TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA ACTTGGTCTG
     4861 ACAGTTACCA ATGCTTAATC AGTGAGGCAC CTATCTCAGC GATCTGTCTA TTTCGTTCAT
     4921 CCATAGTTGC CTGACTCCCC GTCGTGTAGA TAACTACGAT ACGGGAGGGC TTACCATCTG
     4981 GCCCCAGTGC TGCAATGATA CCGCGAGAAC CACGCTCACC GGCTCCAGAT TTATCAGCAA
     5041 TAAACCAGCC AGCCGGAAGG GCCGAGCGCA GAAGTGGTCC TGCAACTTTA TCCGCCTCCA
     5101 TCCAGTCTAT TAATTGTTGC CGGGAAGCTA GAGTAAGTAG TTCGCCAGTT AATAGTTTGC
     5161 GCAACGTTGT TGCCATTGCT ACAGGCATCG TGGTGTCACG CTCGTCGTTT GGTATGGCTT
     5221 CATTCAGCTC CGGTTCCCAA CGATCAAGGC GAGTTACATG ATCCCCCATG TTGTGCAAAA
     5281 AAGCGGTTAG CTCCTTCGGT CCTCCGATCG TTGTCAGAAG TAAGTTGGCC GCAGTGTTAT
     5341 CACTCATGGT TATGGCAGCA CTGCATAATT CTCTTACTGT CATGCCATCC GTAAGATGCT
     5401 TTTCTGTGAC TGGTGAGTAC TCAACCAAGT CATTCTGAGA ATAGTGTATG CGGCGACCGA
     5461 GTTGCTCTTG CCCGGCGTCA ATACGGGATA ATACCGCGCC ACATAGCAGA ACTTTAAAAG
     5521 TGCTCATCAT TGGAAAACGT TCTTCGGGGC GAAAACTCTC AAGGATCTTA CCGCTGTTGA
     5581 GATCCAGTTC GATGTAACCC ACTCGTGCAC CCAACTGATC TTCAGCATCT TTTACTTTCA
     5641 CCAGCGTTTC TGGGTGAGCA AAAACAGGAA GGCAAAATGC CGCAAAAAAG GGAATAAGGG
     5701 CGACACGGAA ATGTTGAATA CTCATACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC
     5761 AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG
     5821 GGGTTCCGCG CACGAATTGG CCAGCGCTGC CATTTTTGGG GTGAGGCCGT TCGCGGCCGA
     5881 GGGGCGCAGC CCCTGGGGGG ATGGGAGGCC CGCGTTAGCG GGCCGGGAGG GTTCGAGAAG
     5941 GGGGGGCACC CCCCTTCGGC GTGCGCGGTC ACGCGCACAG GGCGCAGCCC TGGTTAAAAA
     6001 CAAGGTTTAT AAATATTGGT TTAAAAGCAG GTTAAAAGAC AGGTTAGCGG TGGCCGAAAA
     6061 ACGGGCGGAA ACCCTTGCAA ATGCTGGATT TTCTGCCTGT GGACAGCCCC TCAAATGTCA
     6121 ATAGGTGCGC CCCTCATCTG TCAGCACTCT GCCCCTCAAG TGTCAAGGAT CGCGCCCCTC
     6181 ATCTGTCAGT AGTCGCGCCC CTCAAGTGTC AATACCGCAG GGCACTTATC CCCAGGCTTG
     6241 TCCACATCAT CTGTGGGAAA CTCGCGTAAA ATCAGGCGTT TTCGCCGATT TGCGAGGCTG
     6301 GCCAGCTCCA CGTCGCCGGC CGAAATCGAG CCTGCCCCTC ATCTGTCAAC GCCGCGCCGG
     6361 GTGAGTCGGC CCCTCAAGTG TCAACGTCCG CCCCTCATCT GTCAGTGAGG GCCAAGTTTT
     6421 CCGCGAGGTA TCCACAACGC CGGCGGCCGC GGTGTCTCGC ACACGGCTTC GACGGCGTTT
     6481 CTGGCGCGTT TGCAGGGCCA TAGACGGCCG CCAGCCCAGC GGCG
//