LOCUS       pEPQD1CB0806_(P3_4X_opa 6642 bp ds-DNA     circular     14-OCT-2020
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    complement(24..174)
                     /label="LB"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     primer          35..57
                     /label="QMD671_GB_LB_seq_F"
                     /note="sequence: ctgtatcgagtggtgattttgtg"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer          89..112
                     /label="214or (L1, REV, LB)"
                     /note="sequence: CTGGTGGCAGGATATATTGTGGTG"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    complement(93..117)
                     /label="LB T-DNA repeat"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     primer          107..127
                     /label="226or (L2, FWD, LB)"
                     /note="sequence: GTGGTGTAAACAAATTGACGC"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     primer          complement(107..128)
                     /label="QMD541_LB_rev_seq(226or_binding)"
                     /note="sequence: AGCGTCAATTTGTTTACACCAC"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_feature    226..334
                     /label="OpattB"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    226..339
                     /label="MinSyn_068"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    257..280
                     /label="repeat seq"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    316..339
                     /label="repeat"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    344..347
                     /label="additional sites for pcr"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    348..456
                     /label="OpattB"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    379..402
                     /label="repeat seq"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    438..461
                     /label="repeat"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    461..509
                     /label="min 35S"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    517..537
                     /label="part of TMV 5'UTR"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    544..569
                     /label="part of TMV 5'UTR"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    590..656
                     /label="TMV omega"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    661..1770
                     /label="CrLAMT loganic acid O-methyltransferase CDS, Catharanthus roseus, EU057974 (domesticated, 1 BbsI and 1 SapI site removed)"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     Terminator      1778..2491
                     /label="Atu ocs terminator, pICH41432"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    complement(2519..2644)
                     /label="RB"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    complement(2537..2561)
                     /label="RB T-DNA repeat"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     primer          complement(2555..2579)
                     /label="QMD315_LB_Level2/M_seq_R"
                     /note="sequence: aacgctcttttctcttaggtttacc"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     primer          complement(2605..2624)
                     /label="227or (L2, REV, RB)"
                     /note="sequence: GGATAAACCTTTTCACGCCC"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     conflict        2626..2644
                     /label="partial overdrive"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     primer          complement(2637..2662)
                     /label="213or (L1, FWD, RB)"
                     /note="sequence: GAACCCTGTGGTTGGCATGCACATAC"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     Origin          complement(2674..4155)
                     /label="RK2"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     primer          complement(2717..2735)
                     /label="QMD314_TrfA_LMoutside_F"
                     /note="sequence: ggtgcattgcaaacgctag"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     CDS             complement(2717..3865)
                     /label="Plasmid replication initiator protein TrfA, P07676 (TRFA_ECOLX)"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    complement(4186..4800)
                     /label="replicon of pMB1"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     rep_origin      complement(4186..4805)
                     /label="pBR322\(from\pUC18)"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     primer          complement(4246..4264)
                     /label="QMD062_pJL1_outside(BR322)_F"
                     /note="sequence: tcgccacctctgacttgag"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer          complement(4248..4267)
                     /label="QMD132_LETprom2_F, 61.4"
                     /note="sequence: gtttcgccacctctgacttg"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer          complement(4277..4300)
                     /label="QMD131_LETprom1_F, 61.7, -3.89 self"
                     /note="sequence: gggaaacgcctggtatctttatag"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     primer_bind     complement(4281..4300)
                     /label="pBR322ori-F"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     primer          4746..4767
                     /label="QMD540_pBR322_seq_R"
                     /note="sequence: ttgtttgcaagcagcagattac"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     CDS             complement(4960..5820)
                     /label="AmpR"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer          complement(5087..5103)
                     /label="QMD047 binding region (57.3)"
                     /note="sequence: TCTCGCGGTATCATTGC"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     promoter        complement(5821..5925)
                     /label="AmpR promoter"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     primer          5865..5892
                     /label="212or (L0 REV)"
                     /note="sequence: GTCTCATGAGCGGATACATATTTGAATG"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     rep_origin      complement(join(6012..6642,1..1))
                     /label="oriV"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
ORIGIN
        1 CGCAAAGGAG ATCCTGATCT GACTGATGGG CTGCCTGTAT CGAGTGGTGA TTTTGTGCCG
       61 AGCTGCCGGT CGGGGAGCTG TTGGCTGGCT GGTGGCAGGA TATATTGTGG TGTAAACAAA
      121 TTGACGCTTA GACAACTTAA TAACACATTG CGGACGTTTT TAATGTACTG GGGTGGATGC
      181 AGTGGGCCCC ACTCTGTGAA GACAAACTAG AATTCGAGCT CGGAGcggtg cgggtgccag
      241 ggcgtgccct tgggctcccc gggcgcgtac tccactcccc gagccggtgc gggtgccagg
      301 gcgtgccctt gggctccccg ggcgcgtact ccactccccC CATggtgcgg tgcgggtgcc
      361 agggcgtgcc cttgggctcc ccgggcgcgt actccactcc ccgagccggt gcgggtgcca
      421 gggcgtgccc ttgggctccc cgggcgcgta ctccactccc cttcgcaaga cccttcctct
      481 atataaggaa gttcatttca tttggagagg actccggtat ttttacaaca attaccacaa
      541 caaaacaaac aacaaacaac attacaattt actattctag tcgaaTACTG TATTTTTACA
      601 ACAATTACCA ACAACAACAA ACAACAAACA ACATTACAAT TACTATTTAC AATTACAATG
      661 GTTGCCACAA TTGATTCCAT TGAAATGCCT GCACTTCCAA CAGCTGTGGA AGCCCACCCA
      721 ATGAAAGGTG GTGATGACTC CCACAGTTAC TCCCAAAATT CTTGCTACCA GAAAGGAGTA
      781 ATTGATGCAG CCAAGGCAGT GATTGTTGAA GCAGTGAATG AAAAATTGGA TCTTGAAAAT
      841 AATCCAATCT TTGATCCAAT CAAACCTTTC CGCATTGCAG ATTTTGGATG TTCAACAGGA
      901 CCAAATACTT TCCACGCTAT GCAAAACATA GTAGAATCAG TTGAAACAAA ATACAAATCA
      961 CTTCAAAAAA CCCCTGAATT CCATGTTTTC TTCAATGATC ATGTTAACAA TGATTTCAAT
     1021 GTTCTCTTTA GATCCCTTCC ACCAAACAGG GAATTTTTTG CTGCTGGTGT TCCTGGATCT
     1081 TTCTACACTA GAGTTTTTCC CAAAAATAGC ATTCATTTTG CTCATTGTTC TTATGCACTT
     1141 CATTGGTTAT CTAAAGTGCC CAAGGAAATT CAAGATAAAA ATTCTTTGGC TTATAATAAG
     1201 GGAAGAATTC ATTACACTGG TACTGAAAAA CATGTTGTTA AGGCTTATTT TGGTCAATTC
     1261 CAAAGAGATT TTGAAGGGTT TTTAAAAGCA AGAGCTCAAG AAATTGTTGT TGGAGGGTTG
     1321 ATGGTGATTC AAATACCTGG GCTTCCTAGT GGTGAAGTCC TTTTCTCAAG GACTGGTGCT
     1381 GGTTTGCTTC ACTTCCTTTT GGGAACTTCC TTGATGGAAT TGGTTAACAA GGGAATCATC
     1441 AATGAAGAAA GTGTAGATTC CTTCAATTTG CCTCAATATC ATCCCTCAGT TGAAGATTTG
     1501 GAAATGGTGA TAGAAATGAA CGATTGTTTC ACAATTGAAA GGGTTGGAAC TTTACCTCAT
     1561 CCCATGAAGA ATTTACCATT TGATGTTCAA AGGACTTCTT TACAAGTAAG AGCAATCATG
     1621 GAATGCATTC TCACTGAACA TTTTGGGGAA AATATTTTGG ATCCATTATT TGAAATCTAC
     1681 ACCAAAAATT TGCAAGAGAA TTTCCATGTT TTTGATAAGG AAATTAGAAA GGATGCAGAT
     1741 TTGTACCTTG TCTTGAAACG CAAGGGAAAT TAAGCTTGTC CTGCTTTAAT GAGATATGCG
     1801 AGAAGCCTAT GATCGCATGA TATTTGCTTT CAATTCTGTT GTGCACGTTG TAAAAAACCT
     1861 GAGCATGTGT AGCTCAGATC CTTACCGCCG GTTTCGGTTC ATTCTAATGA ATATATCACC
     1921 CGTTACTATC GTATTTTTAT GAATAATATT CTCCGTTCAA TTTACTGATT GTACCCTACT
     1981 ACTTATATGT ACAATATTAA AATGAAAACA ATATATTGTG CTGAATAGGT TTATAGCGAC
     2041 ATCTATGATA GAGCGCCACA ATAACAAACA ATTGCGTTTT ATTATTACAA ATCCAATTTT
     2101 AAAAAAAGCG GCAGAACCGG TCAAACCTAA AAGACTGATT ACATAAATCT TATTCAAATT
     2161 TCAAAAGTGC CCCAGGGGCT AGTATCTACG ACACACCGAG CGGCGAACTA ATAACGCTCA
     2221 CTGAAGGGAA CTCCGGTTCC CCGCCGGCGC GCATGGGTGA GATTCCTTGA AGTTGAGTAT
     2281 TGGCCGTCCG CTCTACCGAA AGTTACGGGC ACCATTCAAC CCGGTCCAGC ACGGCGGCCG
     2341 GGTAACCGAC TTGCTGCCCC GAGAATTATG CAGCATTTTT TTGGTGTATG TGGGCCCCAA
     2401 ATGAAGTGCA GGTCAAACCT TGACAGTGAC GACAAATCGT TGGGCGGGTC CAGGGCGAAT
     2461 TTTGCGACAA CATGTCGAGG CTCAGCAGGA CCGCTTTACT TGTCTTCTGC ACGAAGTGGT
     2521 TTAAACTATC AGTGTTTGAC AGGATATATT GGCGGGTAAA CCTAAGAGAA AAGAGCGTTT
     2581 ATTAGAATAA TCGGATATTT AAAAGGGCGT GAAAAGGTTT ATCCGTTCGT CCATTTGTAT
     2641 GTGCATGCCA ACCACAGGGT TCCCCAGATC AGGCGCTGGC TGCTGAACCC CCAGCCGGAA
     2701 CTGACCCCAC AAGGCCCTAG CGTTTGCAAT GCACCAGGTC ATCATTGACC CAGGCGTGTT
     2761 CCACCAGGCC GCTGCCTCGC AACTCTTCGC AGGCTTCGCC GACCTGCTCG CGCCACTTCT
     2821 TCACGCGGGT GGAATCCGAT CCGCACATGA GGCGGAAGGT TTCCAGCTTG AGCGGGTACG
     2881 GCTCCCGGTG CGAGCTGAAA TAGTCGAACA TCCGTCGGGC CGTCGGCGAC AGCTTGCGGT
     2941 ACTTCTCCCA TATGAATTTC GTGTAGTGGT CGCCAGCAAA CAGCACGACG ATTTCCTCGT
     3001 CGATCAGGAC CTGGCAACGG GACGTTTTCT TGCCACGGTC CAGGACGCGG AAGCGGTGCA
     3061 GCAGCGACAC CGATTCCAGG TGCCCAACGC GGTCGGACGT GAAGCCCATC GCCGTCGCCT
     3121 GTAGGCGCGA CAGGCATTCC TCGGCCTTCG TGTAATACCG GCCATTGATC GACCAGCCCA
     3181 GGTCCTGGCA AAGCTCGTAG AACGTGAAGG TGATCGGCTC GCCGATAGGG GTGCGCTTCG
     3241 CGTACTCCAA CACCTGCTGC CACACCAGTT CGTCATCGTC GGCCCGCAGC TCGACGCCGG
     3301 TGTAGGTGAT CTTCACGTCC TTGTTGACGT GGAAAATGAC CTTGTTTTGC AGCGCCTCGC
     3361 GCGGGATTTT CTTGTTGCGC GTGGTGAACA GGGCAGAGCG GGCCGTGTCG TTTGGCATCG
     3421 CTCGCATCGT GTCCGGCCAC GGCGCAATAT CGAACAAGGA AAGCTGCATT TCCTTGATCT
     3481 GCTGCTTCGT GTGTTTCAGC AACGCGGCCT GCTTGGCCTC GCTGACCTGT TTTGCCAGGT
     3541 CCTCGCCGGC GGTTTTTCGC TTCTTGGTCG TCATAGTTCC TCGCGTGTCG ATGGTCATCG
     3601 ACTTCGCCAA ACCTGCCGCC TCCTGTTCAA GACGACGCGA ACGCTCCACG GCGGCCGATG
     3661 GCGCGGGCAG GGCAGGGGGA GCCAGTTGCA CGCTGTCGCG CTCGATCTTG GCCGTAGCTT
     3721 GCTGGACCAT CGAGCCGACG GACTGGAAGG TTTCGCGGGG CGCACGCATG ACGGTGCGGC
     3781 TTGCGATGGT TTCGGCATCC TCGGCGGAAA ACCCCGCGTC GATCAGTTCT TGCCTGTATG
     3841 CCTTCCGGTC AAACGTCCGA TTCATTCACC CTCCTTGCGG GATTGCCCCG ACTCACGCCG
     3901 GGGCAATGTG CCCTTATTCC TGATTTGACC CGCCTGGTGC CTTGGTGTCC AGATAATCCA
     3961 CCTTATCGGC AATGAAGTCG GTCCCGTAGA CCGTCTGGCC GTCCTTCTCG TACTTGGTAT
     4021 TCCGAATCTT GCCCTGCACG AATACCAGCG ACCCCTTGCC CAAATACTTG CCGTGGGCCT
     4081 CGGCCTGAGA GCCAAAACAC TTGATGCGGA AGAAGTCGGT GCGCTCCTGC TTGTCGCCGG
     4141 CATCGTTGCG CCACATCTAG GATCTGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT
     4201 TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG
     4261 GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG
     4321 CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG
     4381 CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC
     4441 CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA
     4501 CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG
     4561 TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC
     4621 TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC
     4681 CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG
     4741 TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT
     4801 GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT
     4861 CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA
     4921 ATCAATCTAA AGTATATATG AGTAAACTTG GTCTGACAGT TACCAATGCT TAATCAGTGA
     4981 GGCACCTATC TCAGCGATCT GTCTATTTCG TTCATCCATA GTTGCCTGAC TCCCCGTCGT
     5041 GTAGATAACT ACGATACGGG AGGGCTTACC ATCTGGCCCC AGTGCTGCAA TGATACCGCG
     5101 AGAACCACGC TCACCGGCTC CAGATTTATC AGCAATAAAC CAGCCAGCCG GAAGGGCCGA
     5161 GCGCAGAAGT GGTCCTGCAA CTTTATCCGC CTCCATCCAG TCTATTAATT GTTGCCGGGA
     5221 AGCTAGAGTA AGTAGTTCGC CAGTTAATAG TTTGCGCAAC GTTGTTGCCA TTGCTACAGG
     5281 CATCGTGGTG TCACGCTCGT CGTTTGGTAT GGCTTCATTC AGCTCCGGTT CCCAACGATC
     5341 AAGGCGAGTT ACATGATCCC CCATGTTGTG CAAAAAAGCG GTTAGCTCCT TCGGTCCTCC
     5401 GATCGTTGTC AGAAGTAAGT TGGCCGCAGT GTTATCACTC ATGGTTATGG CAGCACTGCA
     5461 TAATTCTCTT ACTGTCATGC CATCCGTAAG ATGCTTTTCT GTGACTGGTG AGTACTCAAC
     5521 CAAGTCATTC TGAGAATAGT GTATGCGGCG ACCGAGTTGC TCTTGCCCGG CGTCAATACG
     5581 GGATAATACC GCGCCACATA GCAGAACTTT AAAAGTGCTC ATCATTGGAA AACGTTCTTC
     5641 GGGGCGAAAA CTCTCAAGGA TCTTACCGCT GTTGAGATCC AGTTCGATGT AACCCACTCG
     5701 TGCACCCAAC TGATCTTCAG CATCTTTTAC TTTCACCAGC GTTTCTGGGT GAGCAAAAAC
     5761 AGGAAGGCAA AATGCCGCAA AAAAGGGAAT AAGGGCGACA CGGAAATGTT GAATACTCAT
     5821 ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAAGGT TATTGTCTCA TGAGCGGATA
     5881 CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACGA ATTGGCCAGC
     5941 GCTGCCATTT TTGGGGTGAG GCCGTTCGCG GCCGAGGGGC GCAGCCCCTG GGGGGATGGG
     6001 AGGCCCGCGT TAGCGGGCCG GGAGGGTTCG AGAAGGGGGG GCACCCCCCT TCGGCGTGCG
     6061 CGGTCACGCG CACAGGGCGC AGCCCTGGTT AAAAACAAGG TTTATAAATA TTGGTTTAAA
     6121 AGCAGGTTAA AAGACAGGTT AGCGGTGGCC GAAAAACGGG CGGAAACCCT TGCAAATGCT
     6181 GGATTTTCTG CCTGTGGACA GCCCCTCAAA TGTCAATAGG TGCGCCCCTC ATCTGTCAGC
     6241 ACTCTGCCCC TCAAGTGTCA AGGATCGCGC CCCTCATCTG TCAGTAGTCG CGCCCCTCAA
     6301 GTGTCAATAC CGCAGGGCAC TTATCCCCAG GCTTGTCCAC ATCATCTGTG GGAAACTCGC
     6361 GTAAAATCAG GCGTTTTCGC CGATTTGCGA GGCTGGCCAG CTCCACGTCG CCGGCCGAAA
     6421 TCGAGCCTGC CCCTCATCTG TCAACGCCGC GCCGGGTGAG TCGGCCCCTC AAGTGTCAAC
     6481 GTCCGCCCCT CATCTGTCAG TGAGGGCCAA GTTTTCCGCG AGGTATCCAC AACGCCGGCG
     6541 GCCGCGGTGT CTCGCACACG GCTTCGACGG CGTTTCTGGC GCGTTTGCAG GGCCATAGAC
     6601 GGCCGCCAGC CCAGCGGCGA GGGCAACCAG CCCGGTGAGC GT
//