LOCUS       pEPQD1CB0863_(P5_4X_opa 6659 bp ds-DNA     circular     22-DEC-2020
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    complement(25..175)
                     /label="LB"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    complement(94..118)
                     /label="LB T-DNA repeat"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    227..335
                     /label="OpattB"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    227..340
                     /label="MinSyn_068"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    258..281
                     /label="repeat seq"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    317..340
                     /label="repeat"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    345..348
                     /label="additional sites for pcr"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    349..457
                     /label="OpattB"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    380..403
                     /label="repeat seq"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    439..462
                     /label="repeat"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    462..510
                     /label="min 35S"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    518..538
                     /label="part of TMV 5'UTR"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    545..570
                     /label="part of TMV 5'UTR"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    591..657
                     /label="TMV omega"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    662..2239
                     /label="CrSLS secologanin synthase, Catharanthus roseus KF309242.1 (domesticated, 1 SapI site removed))"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    662..2239
                     /label="name depends on ref: SLS1 Brown et al. PNAS 112(11) 3205-3210 (2015), SLS2 de Bernonville et al. BMC Genomics 16(1) 619 (2015)"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     Terminator      2247..2509
                     /label="Atu nos terminator, pICH41421"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    complement(2520..2525)
                     /label="BpiI"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    complement(2537..2662)
                     /label="RB"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    complement(2555..2579)
                     /label="RB T-DNA repeat"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     conflict        2644..2662
                     /label="partial overdrive"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     Origin          complement(2692..4173)
                     /label="RK2"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     CDS             complement(2735..3883)
                     /label="Plasmid replication initiator protein TrfA, P07676 (TRFA_ECOLX)"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     primer_bind     complement(4299..4318)
                     /label="pBR322ori-F"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     CDS             complement(4978..5838)
                     /label="AmpR"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     rep_origin      complement(join(6030..6659,1..2))
                     /label="oriV"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
ORIGIN
        1 TCGCAAAGGA GATCCTGATC TGACTGATGG GCTGCCTGTA TCGAGTGGTG ATTTTGTGCC
       61 GAGCTGCCGG TCGGGGAGCT GTTGGCTGGC TGGTGGCAGG ATATATTGTG GTGTAAACAA
      121 ATTGACGCTT AGACAACTTA ATAACACATT GCGGACGTTT TTAATGTACT GGGGTGGATG
      181 CAGTGGGCCC CACTCTGTGA AGACAACAGA GAATTCGCAT GCGGAGcggt gcgggtgcca
      241 gggcgtgccc ttgggctccc cgggcgcgta ctccactccc cgagccggtg cgggtgccag
      301 ggcgtgccct tgggctcccc gggcgcgtac tccactcccc CCATggtgcg gtgcgggtgc
      361 cagggcgtgc ccttgggctc cccgggcgcg tactccactc cccgagccgg tgcgggtgcc
      421 agggcgtgcc cttgggctcc ccgggcgcgt actccactcc ccttcgcaag acccttcctc
      481 tatataagga agttcatttc atttggagag gactccggta tttttacaac aattaccaca
      541 acaaaacaaa caacaaacaa cattacaatt tactattcta gtcgaaTACT GTATTTTTAC
      601 AACAATTACC AACAACAACA AACAACAAAC AACATTACAA TTACTATTTA CAATTACAAT
      661 GGAGATGGAT ATGGATATCA TTAGAAAGGC AATTGCTGCC ACTATCTTTG CATTGGTTAT
      721 GGCTTGGGCG TGGAGAGTGT TGGATTGGGC ATGGTTTACT CCTAAGAGGA TCGAGAAACG
      781 TCTAAGGCAG CAAGGTTTTA GAGGAAATCC TTATAGATTC TTGGTTGGAG ATGTTAAGGA
      841 GAGTGGAAAA ATGCATCAAG AAGCCTTGTC TAACCCCATG GAGTTCGACA ACGATATTGT
      901 TCCTCGCCTC ATGCCACATA TTAACCACAC CATAAAAACT TACGGTAGGA ATTCATTTAC
      961 ATGGATGGGA AGGATTCCAA GGATTCATGT TATGGAACCT GAACTTATTA AGGAAGTATT
     1021 GACCCACTCC AGCAAATACC AAAAGAACTT TGATGTTCAC AATCCCCTTG TTAAGTTCCT
     1081 TCTCACTGGA GTTGGAAGCT TTGAAGGGGC AAAATGGTCC AAACACAGAA GAATCATTTC
     1141 CCCTGCCTTC ACTCTTGAGA AACTAAAGTC AATGTTGCCG GCTTTTGCCA TATGCTATCA
     1201 TGACATGTTG ACGAAATGGG AGAAATTAGC AGAAAAAGAA GGATCCCATG AAGTTGATAT
     1261 CTTCCCCACA TTTGATGTTT TGACAAGTGA TGTAATTTCA AAGGTTGCAT TTGGTAGCAC
     1321 ATACGATGAA GGAGGCAAAA TCTTCAGACT ATTGAAAGAA CTCATGGATC TCACAATTGA
     1381 TTGCATGAGA GATGTCTACA TCCCAGGGTG GAGCTACTTG CCAACCAAGA GGAACAAGAG
     1441 GATGAAAGAA ATTAACAAAG AAATCACAGA TATGCTAAGG TTCATCATCA ACAAGAGAAT
     1501 GAAGGCTTTG AAGGCTGGAG AACCAGGTGA GGATGACTTG TTGGGAGTCC TGTTGGAATC
     1561 AAACATTCAA GAAATTCAGA AACAAGGAAA CAGGAAGGAT GGTGGAATGA CAATCAACGA
     1621 TGTGATTGAG GAATGCAAAT TGTTCTACTT TGCTGGTCAA GAAACCACCG GAGTTTTGCT
     1681 CACATGGACC ACCATTTTAT TGAGCAAGCA TCCCGAGTGG CAAGAGCGAG CTAGGGAAGA
     1741 AGTTCTCCAA GCCTTTGGCA AGAACAAGCC TGAATTTGAA CGCTTGAATC ATCTTAAATA
     1801 TGTTTCTATG ATCTTATACG AGGTTCTAAG GTTGTACCCG CCAGTTATTG ATCTGACCAA
     1861 GATTATTCAC GAGGACACAA AGTTAGGGCC GTACACAATC CCTGCAGGAA CACAAGTAAT
     1921 GTTGCCGACA GTAATGCTTC ATAGAGAGAA GTCCATTTGG GGAGAAGATG CAATGGAATT
     1981 CAACCCAATG AGATTTGCTG ATGGAGTTGC CAATGCCACC AAGAACAATG TCACATATTT
     2041 GCCATTCAGT TGGGGACCTA GAGTTTGCCT CGGCCAAAAC TTTGCACTTC TTCAAGCAAA
     2101 ATTAGGGTTG GCAATGATTT TACAACGCTT CAAATTTGAT GTAGCTCCAT CCTATGTTCA
     2161 TGCTCCCTTT ACCATTCTTA CAGTTCAACC TCAGTTTGGT TCTCATGTTA TCTACAAGAA
     2221 GCTTGAGAGA CAGAATTTTT AAGCTTGTCA AGCAGATCGT TCAAACATTT GGCAATAAAG
     2281 TTTCTTAAGA TTGAATCCTG TTGCCGGTCT TGCGATGATT ATCATATAAT TTCTGTTGAA
     2341 TTACGTTAAG CATGTAATAA TTAACATGTA ATGCATGACG TTATTTATGA GATGGGTTTT
     2401 TATGATTAGA GTCCCGCAAT TATACATTTA ATACGCGATA GAAAACAAAA TATAGCGCGC
     2461 AAACTAGGAT AAATTATCGC GCGCGGTGTC ATCTATGTTA CTAGATCGAC GCTTGTGTTG
     2521 TCTTCTGCAC GAAGTGGTTT AAACTATCAG TGTTTGACAG GATATATTGG CGGGTAAACC
     2581 TAAGAGAAAA GAGCGTTTAT TAGAATAATC GGATATTTAA AAGGGCGTGA AAAGGTTTAT
     2641 CCGTTCGTCC ATTTGTATGT GCATGCCAAC CACAGGGTTC CCCAGATCAG GCGCTGGCTG
     2701 CTGAACCCCC AGCCGGAACT GACCCCACAA GGCCCTAGCG TTTGCAATGC ACCAGGTCAT
     2761 CATTGACCCA GGCGTGTTCC ACCAGGCCGC TGCCTCGCAA CTCTTCGCAG GCTTCGCCGA
     2821 CCTGCTCGCG CCACTTCTTC ACGCGGGTGG AATCCGATCC GCACATGAGG CGGAAGGTTT
     2881 CCAGCTTGAG CGGGTACGGC TCCCGGTGCG AGCTGAAATA GTCGAACATC CGTCGGGCCG
     2941 TCGGCGACAG CTTGCGGTAC TTCTCCCATA TGAATTTCGT GTAGTGGTCG CCAGCAAACA
     3001 GCACGACGAT TTCCTCGTCG ATCAGGACCT GGCAACGGGA CGTTTTCTTG CCACGGTCCA
     3061 GGACGCGGAA GCGGTGCAGC AGCGACACCG ATTCCAGGTG CCCAACGCGG TCGGACGTGA
     3121 AGCCCATCGC CGTCGCCTGT AGGCGCGACA GGCATTCCTC GGCCTTCGTG TAATACCGGC
     3181 CATTGATCGA CCAGCCCAGG TCCTGGCAAA GCTCGTAGAA CGTGAAGGTG ATCGGCTCGC
     3241 CGATAGGGGT GCGCTTCGCG TACTCCAACA CCTGCTGCCA CACCAGTTCG TCATCGTCGG
     3301 CCCGCAGCTC GACGCCGGTG TAGGTGATCT TCACGTCCTT GTTGACGTGG AAAATGACCT
     3361 TGTTTTGCAG CGCCTCGCGC GGGATTTTCT TGTTGCGCGT GGTGAACAGG GCAGAGCGGG
     3421 CCGTGTCGTT TGGCATCGCT CGCATCGTGT CCGGCCACGG CGCAATATCG AACAAGGAAA
     3481 GCTGCATTTC CTTGATCTGC TGCTTCGTGT GTTTCAGCAA CGCGGCCTGC TTGGCCTCGC
     3541 TGACCTGTTT TGCCAGGTCC TCGCCGGCGG TTTTTCGCTT CTTGGTCGTC ATAGTTCCTC
     3601 GCGTGTCGAT GGTCATCGAC TTCGCCAAAC CTGCCGCCTC CTGTTCAAGA CGACGCGAAC
     3661 GCTCCACGGC GGCCGATGGC GCGGGCAGGG CAGGGGGAGC CAGTTGCACG CTGTCGCGCT
     3721 CGATCTTGGC CGTAGCTTGC TGGACCATCG AGCCGACGGA CTGGAAGGTT TCGCGGGGCG
     3781 CACGCATGAC GGTGCGGCTT GCGATGGTTT CGGCATCCTC GGCGGAAAAC CCCGCGTCGA
     3841 TCAGTTCTTG CCTGTATGCC TTCCGGTCAA ACGTCCGATT CATTCACCCT CCTTGCGGGA
     3901 TTGCCCCGAC TCACGCCGGG GCAATGTGCC CTTATTCCTG ATTTGACCCG CCTGGTGCCT
     3961 TGGTGTCCAG ATAATCCACC TTATCGGCAA TGAAGTCGGT CCCGTAGACC GTCTGGCCGT
     4021 CCTTCTCGTA CTTGGTATTC CGAATCTTGC CCTGCACGAA TACCAGCGAC CCCTTGCCCA
     4081 AATACTTGCC GTGGGCCTCG GCCTGAGAGC CAAAACACTT GATGCGGAAG AAGTCGGTGC
     4141 GCTCCTGCTT GTCGCCGGCA TCGTTGCGCC ACATCTAGGA TCTGCCAGGA ACCGTAAAAA
     4201 GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG
     4261 ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC
     4321 TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC
     4381 CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC
     4441 GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG
     4501 CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC
     4561 ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA
     4621 GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGG ACAGTATTTG GTATCTGCGC
     4681 TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC
     4741 CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG
     4801 ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC
     4861 ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA
     4921 TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAAACTTGGT CTGACAGTTA
     4981 CCAATGCTTA ATCAGTGAGG CACCTATCTC AGCGATCTGT CTATTTCGTT CATCCATAGT
     5041 TGCCTGACTC CCCGTCGTGT AGATAACTAC GATACGGGAG GGCTTACCAT CTGGCCCCAG
     5101 TGCTGCAATG ATACCGCGAG AACCACGCTC ACCGGCTCCA GATTTATCAG CAATAAACCA
     5161 GCCAGCCGGA AGGGCCGAGC GCAGAAGTGG TCCTGCAACT TTATCCGCCT CCATCCAGTC
     5221 TATTAATTGT TGCCGGGAAG CTAGAGTAAG TAGTTCGCCA GTTAATAGTT TGCGCAACGT
     5281 TGTTGCCATT GCTACAGGCA TCGTGGTGTC ACGCTCGTCG TTTGGTATGG CTTCATTCAG
     5341 CTCCGGTTCC CAACGATCAA GGCGAGTTAC ATGATCCCCC ATGTTGTGCA AAAAAGCGGT
     5401 TAGCTCCTTC GGTCCTCCGA TCGTTGTCAG AAGTAAGTTG GCCGCAGTGT TATCACTCAT
     5461 GGTTATGGCA GCACTGCATA ATTCTCTTAC TGTCATGCCA TCCGTAAGAT GCTTTTCTGT
     5521 GACTGGTGAG TACTCAACCA AGTCATTCTG AGAATAGTGT ATGCGGCGAC CGAGTTGCTC
     5581 TTGCCCGGCG TCAATACGGG ATAATACCGC GCCACATAGC AGAACTTTAA AAGTGCTCAT
     5641 CATTGGAAAA CGTTCTTCGG GGCGAAAACT CTCAAGGATC TTACCGCTGT TGAGATCCAG
     5701 TTCGATGTAA CCCACTCGTG CACCCAACTG ATCTTCAGCA TCTTTTACTT TCACCAGCGT
     5761 TTCTGGGTGA GCAAAAACAG GAAGGCAAAA TGCCGCAAAA AAGGGAATAA GGGCGACACG
     5821 GAAATGTTGA ATACTCATAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA
     5881 TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC
     5941 GCGCACGAAT TGGCCAGCGC TGCCATTTTT GGGGTGAGGC CGTTCGCGGC CGAGGGGCGC
     6001 AGCCCCTGGG GGGATGGGAG GCCCGCGTTA GCGGGCCGGG AGGGTTCGAG AAGGGGGGGC
     6061 ACCCCCCTTC GGCGTGCGCG GTCACGCGCA CAGGGCGCAG CCCTGGTTAA AAACAAGGTT
     6121 TATAAATATT GGTTTAAAAG CAGGTTAAAA GACAGGTTAG CGGTGGCCGA AAAACGGGCG
     6181 GAAACCCTTG CAAATGCTGG ATTTTCTGCC TGTGGACAGC CCCTCAAATG TCAATAGGTG
     6241 CGCCCCTCAT CTGTCAGCAC TCTGCCCCTC AAGTGTCAAG GATCGCGCCC CTCATCTGTC
     6301 AGTAGTCGCG CCCCTCAAGT GTCAATACCG CAGGGCACTT ATCCCCAGGC TTGTCCACAT
     6361 CATCTGTGGG AAACTCGCGT AAAATCAGGC GTTTTCGCCG ATTTGCGAGG CTGGCCAGCT
     6421 CCACGTCGCC GGCCGAAATC GAGCCTGCCC CTCATCTGTC AACGCCGCGC CGGGTGAGTC
     6481 GGCCCCTCAA GTGTCAACGT CCGCCCCTCA TCTGTCAGTG AGGGCCAAGT TTTCCGCGAG
     6541 GTATCCACAA CGCCGGCGGC CGCGGTGTCT CGCACACGGC TTCGACGGCG TTTCTGGCGC
     6601 GTTTGCAGGG CCATAGACGG CCGCCAGCCC AGCGGCGAGG GCAACCAGCC CGGTGAGCG
//