LOCUS       pEPQD1CB0808_(P4_4X_opa 6531 bp ds-DNA     circular     20-OCT-2020
DEFINITION  .
FEATURES             Location/Qualifiers
     CDS             complement(264..1124)
                     /label="AmpR"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     rep_origin      complement(1316..1947)
                     /label="oriV"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    complement(1970..2120)
                     /label="LB"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    complement(2039..2063)
                     /label="LB T-DNA repeat"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    2172..2280
                     /label="OpattB"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    2172..2285
                     /label="MinSyn_068"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    2203..2226
                     /label="repeat seq"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    2262..2285
                     /label="repeat"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    2290..2293
                     /label="additional sites for pcr"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    2294..2402
                     /label="OpattB"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    2325..2348
                     /label="repeat seq"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    2384..2407
                     /label="repeat"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    2407..2455
                     /label="min 35S"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    2463..2483
                     /label="part of TMV 5'UTR"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    2490..2515
                     /label="part of TMV 5'UTR"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    2536..2602
                     /label="TMV omega"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     None            2607..4103
                     /label="CrTDC tryptophan decarboxylase CDS, Catharanthus roseus, M25151 (domesticated, 1 BtgZI site removed)"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     terminator      4111..4326
                     /label="Atu g7 terminator from pICH72400"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    complement(4354..4479)
                     /label="RB"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    complement(4372..4396)
                     /label="RB T-DNA repeat"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     conflict        4461..4479
                     /label="partial overdrive"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     Origin          complement(4509..5990)
                     /label="RK2"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     CDS             complement(4552..5700)
                     /label="Plasmid replication initiator protein TrfA, P07676 (TRFA_ECOLX)"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     primer_bind     complement(6116..6135)
                     /label="pBR322ori-F"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
ORIGIN
        1 GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG
       61 CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG
      121 TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA
      181 AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA
      241 TATGAGTAAA CTTGGTCTGA CAGTTACCAA TGCTTAATCA GTGAGGCACC TATCTCAGCG
      301 ATCTGTCTAT TTCGTTCATC CATAGTTGCC TGACTCCCCG TCGTGTAGAT AACTACGATA
      361 CGGGAGGGCT TACCATCTGG CCCCAGTGCT GCAATGATAC CGCGAGAACC ACGCTCACCG
      421 GCTCCAGATT TATCAGCAAT AAACCAGCCA GCCGGAAGGG CCGAGCGCAG AAGTGGTCCT
      481 GCAACTTTAT CCGCCTCCAT CCAGTCTATT AATTGTTGCC GGGAAGCTAG AGTAAGTAGT
      541 TCGCCAGTTA ATAGTTTGCG CAACGTTGTT GCCATTGCTA CAGGCATCGT GGTGTCACGC
      601 TCGTCGTTTG GTATGGCTTC ATTCAGCTCC GGTTCCCAAC GATCAAGGCG AGTTACATGA
      661 TCCCCCATGT TGTGCAAAAA AGCGGTTAGC TCCTTCGGTC CTCCGATCGT TGTCAGAAGT
      721 AAGTTGGCCG CAGTGTTATC ACTCATGGTT ATGGCAGCAC TGCATAATTC TCTTACTGTC
      781 ATGCCATCCG TAAGATGCTT TTCTGTGACT GGTGAGTACT CAACCAAGTC ATTCTGAGAA
      841 TAGTGTATGC GGCGACCGAG TTGCTCTTGC CCGGCGTCAA TACGGGATAA TACCGCGCCA
      901 CATAGCAGAA CTTTAAAAGT GCTCATCATT GGAAAACGTT CTTCGGGGCG AAAACTCTCA
      961 AGGATCTTAC CGCTGTTGAG ATCCAGTTCG ATGTAACCCA CTCGTGCACC CAACTGATCT
     1021 TCAGCATCTT TTACTTTCAC CAGCGTTTCT GGGTGAGCAA AAACAGGAAG GCAAAATGCC
     1081 GCAAAAAAGG GAATAAGGGC GACACGGAAA TGTTGAATAC TCATACTCTT CCTTTTTCAA
     1141 TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT
     1201 TAGAAAAATA AACAAATAGG GGTTCCGCGC ACGAATTGGC CAGCGCTGCC ATTTTTGGGG
     1261 TGAGGCCGTT CGCGGCCGAG GGGCGCAGCC CCTGGGGGGA TGGGAGGCCC GCGTTAGCGG
     1321 GCCGGGAGGG TTCGAGAAGG GGGGGCACCC CCCTTCGGCG TGCGCGGTCA CGCGCACAGG
     1381 GCGCAGCCCT GGTTAAAAAC AAGGTTTATA AATATTGGTT TAAAAGCAGG TTAAAAGACA
     1441 GGTTAGCGGT GGCCGAAAAA CGGGCGGAAA CCCTTGCAAA TGCTGGATTT TCTGCCTGTG
     1501 GACAGCCCCT CAAATGTCAA TAGGTGCGCC CCTCATCTGT CAGCACTCTG CCCCTCAAGT
     1561 GTCAAGGATC GCGCCCCTCA TCTGTCAGTA GTCGCGCCCC TCAAGTGTCA ATACCGCAGG
     1621 GCACTTATCC CCAGGCTTGT CCACATCATC TGTGGGAAAC TCGCGTAAAA TCAGGCGTTT
     1681 TCGCCGATTT GCGAGGCTGG CCAGCTCCAC GTCGCCGGCC GAAATCGAGC CTGCCCCTCA
     1741 TCTGTCAACG CCGCGCCGGG TGAGTCGGCC CCTCAAGTGT CAACGTCCGC CCCTCATCTG
     1801 TCAGTGAGGG CCAAGTTTTC CGCGAGGTAT CCACAACGCC GGCGGCCGCG GTGTCTCGCA
     1861 CACGGCTTCG ACGGCGTTTC TGGCGCGTTT GCAGGGCCAT AGACGGCCGC CAGCCCAGCG
     1921 GCGAGGGCAA CCAGCCCGGT GAGCGTCGCA AAGGAGATCC TGATCTGACT GATGGGCTGC
     1981 CTGTATCGAG TGGTGATTTT GTGCCGAGCT GCCGGTCGGG GAGCTGTTGG CTGGCTGGTG
     2041 GCAGGATATA TTGTGGTGTA AACAAATTGA CGCTTAGACA ACTTAATAAC ACATTGCGGA
     2101 CGTTTTTAAT GTACTGGGGT GGATGCAGTG GGCCCCACTC TGTGAAGACA ATTACGAATT
     2161 CCCATGGGGA Gcggtgcggg tgccagggcg tgcccttggg ctccccgggc gcgtactcca
     2221 ctccccgagc cggtgcgggt gccagggcgt gcccttgggc tccccgggcg cgtactccac
     2281 tccccCCATg gtgcggtgcg ggtgccaggg cgtgcccttg ggctccccgg gcgcgtactc
     2341 cactccccga gccggtgcgg gtgccagggc gtgcccttgg gctccccggg cgcgtactcc
     2401 actccccttc gcaagaccct tcctctatat aaggaagttc atttcatttg gagaggactc
     2461 cggtattttt acaacaatta ccacaacaaa acaaacaaca aacaacatta caatttacta
     2521 ttctagtcga aTACTGTATT TTTACAACAA TTACCAACAA CAACAAACAA CAAACAACAT
     2581 TACAATTACT ATTTACAATT ACAATGGGCA GCATTGATTC AACAAATGTA GCCATGTCCA
     2641 ATTCTCCAGT TGGAGAATTT AAGCCACTTG AAGCTGAGGA ATTCCGAAAA CAAGCCCATC
     2701 GTATGGTAGA TTTCATAGCC GATTATTACA AAAATGTGGA AACATATCCG GTCCTTAGCG
     2761 AAGTCGAACC TGGATATCTC CGAAAACGTA TCCCCGAAAC CGCTCCTTAC CTCCCCGAAC
     2821 CACTTGACGA CATCATGAAA GATATTCAGA AGGATATTAT CCCAGGAATG ACAAATTGGA
     2881 TGAGCCCTAA TTTTTATGCA TTTTTTCCTG CCACTGTTAG TTCAGCTGCC TTTTTAGGAG
     2941 AAATGTTGTC TACTGCCCTA AATTCAGTAG GCTTTACTTG GGTTTCTTCA CCAGCCGCCA
     3001 CCGAATTAGA AATGATTGTT ATGGATTGGT TGGCTCAGAT CCTTAAACTC CCCAAATCTT
     3061 TCATGTTTTC AGGTACCGGT GGCGGCGTCA TCCAAAACAC CACTAGCGAG TCCATTCTTT
     3121 GTACAATCAT TGCCGCCCGG GAAAGGGCCC TGGAGAAGCT CGGTCCCGAT AGTATTGGAA
     3181 AACTTGTCTG TTACGGATCC GATCAAACCC ATACCATGTT CCCCAAAACT TGCAAATTGG
     3241 CGGGAATTTA TCCGAATAAT ATTAGGTTAA TACCTACGAC CGTCGAAACG GATTTCGGCA
     3301 TCTCACCTCA AGTTCTACGA AAAATGGTCG AGGATGACGT GGCGGCCGGA TATGTACCGC
     3361 TGTTCTTATG CGCTACCCTG GGTACCACCT CGACCACGGC TACCGATCCT GTGGACTCAC
     3421 TTTCTGAAAT CGCTAACGAG TTTGGTATTT GGATCCACGT GGATGCTGCT TATGCGGGAA
     3481 GCGCCTGTAT ATGTCCCGAG TTTAGACATT ACTTGGATGG AATCGAACGA GTTGACTCAC
     3541 TGAGTCTGAG TCCACACAAA TGGCTACTCG CTTACTTAGA TTGCACTTGC TTGTGGGTCA
     3601 AGCAACCACA TTTGTTACTA AGGGCACTCA CTACGAATCC TGAGTATTTA AAAAATAAAC
     3661 AGAGTGATTT AGACAAAGTT GTGGACTTCA AAAATTGGCA AATCGCAACG GGACGAAAAT
     3721 TTCGGTCGCT GAAACTTTGG CTCATTTTAC GTAGCTATGG AGTTGTTAAT TTACAGAGTC
     3781 ATATTCGTTC TGACGTCGCA ATGGGCAAAA TGTTCGAAGA ATGGGTTAGA TCAGACTCCA
     3841 GATTCGAAAT TGTGGTACCG AGAAACTTTT CTCTTGTTTG TTTTAGATTA AAACCTGACG
     3901 TTTCGAGTTT ACATGTAGAA GAAGTGAATA AGAAACTTTT GGACATGCTT AACTCGACGG
     3961 GACGAGTTTA TATGACTCAT ACTATTGTGG GAGGCATATA CATGCTAAGA CTGGCTGTTG
     4021 GCTCGTCACT AACTGAAGAA CATCATGTAC GCCGTGTTTG GGATTTGATT CAAAAATTAA
     4081 CCGATGATTT GCTCAAAGAA GCTTGAGCTT gatcccccgt cgacagctag ctatatcatc
     4141 aatttatgta ttacacataa tatcgcactc agtctttcat ctacggcaat gtaccagctg
     4201 atataatcag ttattgaaat atttctgaat ttaaacttgc atcaataaat ttatgttttt
     4261 gcttggacta taatacctga cttgttattt tatcaataaa tatttaaact atatttcttt
     4321 caagatcgct CAGATTGTCT TCTGCACGAA GTGGTTTAAA CTATCAGTGT TTGACAGGAT
     4381 ATATTGGCGG GTAAACCTAA GAGAAAAGAG CGTTTATTAG AATAATCGGA TATTTAAAAG
     4441 GGCGTGAAAA GGTTTATCCG TTCGTCCATT TGTATGTGCA TGCCAACCAC AGGGTTCCCC
     4501 AGATCAGGCG CTGGCTGCTG AACCCCCAGC CGGAACTGAC CCCACAAGGC CCTAGCGTTT
     4561 GCAATGCACC AGGTCATCAT TGACCCAGGC GTGTTCCACC AGGCCGCTGC CTCGCAACTC
     4621 TTCGCAGGCT TCGCCGACCT GCTCGCGCCA CTTCTTCACG CGGGTGGAAT CCGATCCGCA
     4681 CATGAGGCGG AAGGTTTCCA GCTTGAGCGG GTACGGCTCC CGGTGCGAGC TGAAATAGTC
     4741 GAACATCCGT CGGGCCGTCG GCGACAGCTT GCGGTACTTC TCCCATATGA ATTTCGTGTA
     4801 GTGGTCGCCA GCAAACAGCA CGACGATTTC CTCGTCGATC AGGACCTGGC AACGGGACGT
     4861 TTTCTTGCCA CGGTCCAGGA CGCGGAAGCG GTGCAGCAGC GACACCGATT CCAGGTGCCC
     4921 AACGCGGTCG GACGTGAAGC CCATCGCCGT CGCCTGTAGG CGCGACAGGC ATTCCTCGGC
     4981 CTTCGTGTAA TACCGGCCAT TGATCGACCA GCCCAGGTCC TGGCAAAGCT CGTAGAACGT
     5041 GAAGGTGATC GGCTCGCCGA TAGGGGTGCG CTTCGCGTAC TCCAACACCT GCTGCCACAC
     5101 CAGTTCGTCA TCGTCGGCCC GCAGCTCGAC GCCGGTGTAG GTGATCTTCA CGTCCTTGTT
     5161 GACGTGGAAA ATGACCTTGT TTTGCAGCGC CTCGCGCGGG ATTTTCTTGT TGCGCGTGGT
     5221 GAACAGGGCA GAGCGGGCCG TGTCGTTTGG CATCGCTCGC ATCGTGTCCG GCCACGGCGC
     5281 AATATCGAAC AAGGAAAGCT GCATTTCCTT GATCTGCTGC TTCGTGTGTT TCAGCAACGC
     5341 GGCCTGCTTG GCCTCGCTGA CCTGTTTTGC CAGGTCCTCG CCGGCGGTTT TTCGCTTCTT
     5401 GGTCGTCATA GTTCCTCGCG TGTCGATGGT CATCGACTTC GCCAAACCTG CCGCCTCCTG
     5461 TTCAAGACGA CGCGAACGCT CCACGGCGGC CGATGGCGCG GGCAGGGCAG GGGGAGCCAG
     5521 TTGCACGCTG TCGCGCTCGA TCTTGGCCGT AGCTTGCTGG ACCATCGAGC CGACGGACTG
     5581 GAAGGTTTCG CGGGGCGCAC GCATGACGGT GCGGCTTGCG ATGGTTTCGG CATCCTCGGC
     5641 GGAAAACCCC GCGTCGATCA GTTCTTGCCT GTATGCCTTC CGGTCAAACG TCCGATTCAT
     5701 TCACCCTCCT TGCGGGATTG CCCCGACTCA CGCCGGGGCA ATGTGCCCTT ATTCCTGATT
     5761 TGACCCGCCT GGTGCCTTGG TGTCCAGATA ATCCACCTTA TCGGCAATGA AGTCGGTCCC
     5821 GTAGACCGTC TGGCCGTCCT TCTCGTACTT GGTATTCCGA ATCTTGCCCT GCACGAATAC
     5881 CAGCGACCCC TTGCCCAAAT ACTTGCCGTG GGCCTCGGCC TGAGAGCCAA AACACTTGAT
     5941 GCGGAAGAAG TCGGTGCGCT CCTGCTTGTC GCCGGCATCG TTGCGCCACA TCTAGGATCT
     6001 GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC
     6061 GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA
     6121 TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT
     6181 ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC
     6241 TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC
     6301 CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA
     6361 AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT
     6421 GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGGACA
     6481 GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT T
//