LOCUS       pEPQD1CB0802_(P1_4X_opa 6075 bp ds-DNA     circular     20-OCT-2020
DEFINITION  .
FEATURES             Location/Qualifiers
     rep_origin      complement(86..717)
                     /label="oriV"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     Misc._feature   739..886
                     /label="LB"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    complement(809..833)
                     /label="LB T-DNA repeat"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    942..1050
                     /label="OpattB"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    942..1055
                     /label="MinSyn_068"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    973..996
                     /label="repeat seq"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    1032..1055
                     /label="repeat"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    1060..1063
                     /label="additional sites for pcr"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    1064..1172
                     /label="OpattB"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    1095..1118
                     /label="repeat seq"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    1154..1177
                     /label="repeat"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    1177..1225
                     /label="min 35S"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    1233..1253
                     /label="part of TMV 5'UTR"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    1260..1285
                     /label="part of TMV 5'UTR"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    1306..1372
                     /label="TMV omega"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    1377..2429
                     /label="CrSTR2 strictosidine synthase, Catharanthus roseus, X61932 (domesticated, 1 BbsI site removed, L19-S32 recoded to avoid repeats)"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     Terminator      2437..2640
                     /label="CaMV 35S terminator, pICH41414"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     Misc._feature   complement(2672..2799)
                     /label="RB short"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    complement(2686..2710)
                     /label="RB T-DNA repeat"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     conflict        2775..2793
                     /label="partial overdrive"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     Origin          complement(2823..4304)
                     /label="RK2"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     CDS             complement(2866..4014)
                     /label="Plasmid replication initiator protein TrfA, P07676 (TRFA_ECOLX)"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    complement(4335..4949)
                     /label="replicon of pMB1"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     rep_origin      complement(4335..4954)
                     /label="pBR322\(from\pUC18)"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     primer_bind     complement(4430..4449)
                     /label="pBR322ori-F"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     CDS             complement(5109..5969)
                     /label="AmpR"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     promoter        complement(5970..6074)
                     /label="AmpR promoter"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
ORIGIN
        1 ACGAATTGGC CAGCGCTGCC ATTTTTGGGG TGAGGCCGTT CGCGGCCGAG GGGCGCAGCC
       61 CCTGGGGGGA TGGGAGGCCC GCGTTAGCGG GCCGGGAGGG TTCGAGAAGG GGGGGCACCC
      121 CCCTTCGGCG TGCGCGGTCA CGCGCACAGG GCGCAGCCCT GGTTAAAAAC AAGGTTTATA
      181 AATATTGGTT TAAAAGCAGG TTAAAAGACA GGTTAGCGGT GGCCGAAAAA CGGGCGGAAA
      241 CCCTTGCAAA TGCTGGATTT TCTGCCTGTG GACAGCCCCT CAAATGTCAA TAGGTGCGCC
      301 CCTCATCTGT CAGCACTCTG CCCCTCAAGT GTCAAGGATC GCGCCCCTCA TCTGTCAGTA
      361 GTCGCGCCCC TCAAGTGTCA ATACCGCAGG GCACTTATCC CCAGGCTTGT CCACATCATC
      421 TGTGGGAAAC TCGCGTAAAA TCAGGCGTTT TCGCCGATTT GCGAGGCTGG CCAGCTCCAC
      481 GTCGCCGGCC GAAATCGAGC CTGCCCCTCA TCTGTCAACG CCGCGCCGGG TGAGTCGGCC
      541 CCTCAAGTGT CAACGTCCGC CCCTCATCTG TCAGTGAGGG CCAAGTTTTC CGCGAGGTAT
      601 CCACAACGCC GGCGGCCGCG GTGTCTCGCA CACGGCTTCG ACGGCGTTTC TGGCGCGTTT
      661 GCAGGGCCAT AGACGGCCGC CAGCCCAGCG GCGAGGGCAA CCAGCCCGGT GAGCGTCGCA
      721 AAGGAGATCC TGATCTGACT GATGGGCTGC CTGTATCGAG TGGTGATTTT GTGCCGAGCT
      781 GCCGGTCGGG GAGCTGTTGG CTGGCTGGTG GCAGGATATA TTGTGGTGTA AACAAATTGA
      841 CGCTTAGACA ACTTAATAAC ACATTGCGGA CGTTTTTAAT GTACTGGGGT GGATGCAGTG
      901 GGCCCCACTC TGTGAAGACA ATGCCGAATT CGGATCCGGA Gcggtgcggg tgccagggcg
      961 tgcccttggg ctccccgggc gcgtactcca ctccccgagc cggtgcgggt gccagggcgt
     1021 gcccttgggc tccccgggcg cgtactccac tccccCCATg gtgcggtgcg ggtgccaggg
     1081 cgtgcccttg ggctccccgg gcgcgtactc cactccccga gccggtgcgg gtgccagggc
     1141 gtgcccttgg gctccccggg cgcgtactcc actccccttc gcaagaccct tcctctatat
     1201 aaggaagttc atttcatttg gagaggactc cggtattttt acaacaatta ccacaacaaa
     1261 acaaacaaca aacaacatta caatttacta ttctagtcga aTACTGTATT TTTACAACAA
     1321 TTACCAACAA CAACAAACAA CAAACAACAT TACAATTACT ATTTACAATT ACAATGGCAA
     1381 ACTTTTCTGA ATCTAAATCC ATGATGGCAG TTTTCTTCAT GTTTTTCCTG CTTCTTTTGT
     1441 CATCTTCTAG TTCTTCATCT TCAAGTTCAC CAATTTTGAA AAAGATTTTT ATTGAAAGCC
     1501 CTTCCTATGC TCCGAATGCC TTCACCTTCG ATTCAACTGA TAAAGGGTTC TACACTTCCG
     1561 TCCAAGATGG CCGAGTTATC AAATATGAAG GGCCAAATTC AGGCTTCACT GACTTCGCCT
     1621 ACGCATCTCC CTTCTGGAAC AAAGCTTTTT GTGAGAACAG CACCGATCCA GAGAAAAGAC
     1681 CATTGTGTGG GAGGACATAT GATATTTCCT ATGACTATAA GAACAGCCAA ATGTACATTG
     1741 TTGATGGCCA TTACCATCTT TGTGTGGTTG GAAAAGAAGG TGGGTATGCC ACACAACTAG
     1801 CCACAAGTGT GCAAGGAGTG CCATTCAAAT GGCTCTATGC AGTAACTGTT GATCAGAGAA
     1861 CAGGGATTGT TTATTTCACT GATGTTAGCT CCATACATGA TGACAGTCCC GAAGGTGTGG
     1921 AAGAAATCAT GAATACAAGT GATAGAACAG GGAGATTAAT GAAGTATGAT CCTTCAACAA
     1981 AAGAAACCAC CTTATTATTG AAAGAGCTAC ATGTTCCCGG CGGTGCAGAA ATCAGCGCAG
     2041 ATGGTTCCTT TGTTGTAGTA GCAGAATTTT TAAGCAATCG GATAGTGAAG TATTGGCTAG
     2101 AAGGGCCAAA GAAAGGCAGT GCAGAGTTCT TAGTTACAAT CCCAAATCCA GGAAATATAA
     2161 AGAGGAATTC TGATGGCCAT TTTTGGGTGT CATCTAGTGA AGAATTAGAT GGAGGTCAAC
     2221 ATGGAAGAGT TGTTTCAAGA GGAATTAAGT TTGATGGATT TGGGAATATT CTTCAAGTTA
     2281 TACCACTTCC ACCACCATAT GAAGGTGAAC ATTTTGAACA GATTCAAGAG CACGATGGTT
     2341 TGTTATACAT TGGAAGTCTC TTCCATAGCT CTGTGGGTAT ATTAGTGTAT GATGATCATG
     2401 ATAACAAGGG AAATTCTTAT GTTTCTAGCT GAGCTTCTCT AGCTAGAGTC GATCGACAAG
     2461 CTCGAGTTTC TCCATAATAA TGTGTGAGTA GTTCCCAGAT AAGGGAATTA GGGTTCCTAT
     2521 AGGGTTTCGC TCATGTGTTG AGCATATAAG AAACCCTTAG TATGTATTTG TATTTGTAAA
     2581 ATACTTCTAT CAATAAAATT TCTAATTCCT AAAACCAAAA TCCAGTACTA AAATCCAGAT
     2641 CGCTGCAATT GTCTTCTGCA CGAAGTGGTT TAAACTATCA GTGTTTGACA GGATATATTG
     2701 GCGGGTAAAC CTAAGAGAAA AGAGCGTTTA TTAGAATAAT CGGATATTTA AAAGGGCGTG
     2761 AAAAGGTTTA TCCGTTCGTC CATTTGTATG TGCATGCCAA CCACAGGGTT CCCCAGATCA
     2821 GGCGCTGGCT GCTGAACCCC CAGCCGGAAC TGACCCCACA AGGCCCTAGC GTTTGCAATG
     2881 CACCAGGTCA TCATTGACCC AGGCGTGTTC CACCAGGCCG CTGCCTCGCA ACTCTTCGCA
     2941 GGCTTCGCCG ACCTGCTCGC GCCACTTCTT CACGCGGGTG GAATCCGATC CGCACATGAG
     3001 GCGGAAGGTT TCCAGCTTGA GCGGGTACGG CTCCCGGTGC GAGCTGAAAT AGTCGAACAT
     3061 CCGTCGGGCC GTCGGCGACA GCTTGCGGTA CTTCTCCCAT ATGAATTTCG TGTAGTGGTC
     3121 GCCAGCAAAC AGCACGACGA TTTCCTCGTC GATCAGGACC TGGCAACGGG ACGTTTTCTT
     3181 GCCACGGTCC AGGACGCGGA AGCGGTGCAG CAGCGACACC GATTCCAGGT GCCCAACGCG
     3241 GTCGGACGTG AAGCCCATCG CCGTCGCCTG TAGGCGCGAC AGGCATTCCT CGGCCTTCGT
     3301 GTAATACCGG CCATTGATCG ACCAGCCCAG GTCCTGGCAA AGCTCGTAGA ACGTGAAGGT
     3361 GATCGGCTCG CCGATAGGGG TGCGCTTCGC GTACTCCAAC ACCTGCTGCC ACACCAGTTC
     3421 GTCATCGTCG GCCCGCAGCT CGACGCCGGT GTAGGTGATC TTCACGTCCT TGTTGACGTG
     3481 GAAAATGACC TTGTTTTGCA GCGCCTCGCG CGGGATTTTC TTGTTGCGCG TGGTGAACAG
     3541 GGCAGAGCGG GCCGTGTCGT TTGGCATCGC TCGCATCGTG TCCGGCCACG GCGCAATATC
     3601 GAACAAGGAA AGCTGCATTT CCTTGATCTG CTGCTTCGTG TGTTTCAGCA ACGCGGCCTG
     3661 CTTGGCCTCG CTGACCTGTT TTGCCAGGTC CTCGCCGGCG GTTTTTCGCT TCTTGGTCGT
     3721 CATAGTTCCT CGCGTGTCGA TGGTCATCGA CTTCGCCAAA CCTGCCGCCT CCTGTTCAAG
     3781 ACGACGCGAA CGCTCCACGG CGGCCGATGG CGCGGGCAGG GCAGGGGGAG CCAGTTGCAC
     3841 GCTGTCGCGC TCGATCTTGG CCGTAGCTTG CTGGACCATC GAGCCGACGG ACTGGAAGGT
     3901 TTCGCGGGGC GCACGCATGA CGGTGCGGCT TGCGATGGTT TCGGCATCCT CGGCGGAAAA
     3961 CCCCGCGTCG ATCAGTTCTT GCCTGTATGC CTTCCGGTCA AACGTCCGAT TCATTCACCC
     4021 TCCTTGCGGG ATTGCCCCGA CTCACGCCGG GGCAATGTGC CCTTATTCCT GATTTGACCC
     4081 GCCTGGTGCC TTGGTGTCCA GATAATCCAC CTTATCGGCA ATGAAGTCGG TCCCGTAGAC
     4141 CGTCTGGCCG TCCTTCTCGT ACTTGGTATT CCGAATCTTG CCCTGCACGA ATACCAGCGA
     4201 CCCCTTGCCC AAATACTTGC CGTGGGCCTC GGCCTGAGAG CCAAAACACT TGATGCGGAA
     4261 GAAGTCGGTG CGCTCCTGCT TGTCGCCGGC ATCGTTGCGC CACATCTAGG ATCTGCCAGG
     4321 AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT
     4381 CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG
     4441 GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA
     4501 TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG
     4561 TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT
     4621 CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC
     4681 GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC
     4741 GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT
     4801 GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC
     4861 GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC
     4921 AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG
     4981 AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG
     5041 ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA GTAAACTTGG
     5101 TCTGACAGTT ACCAATGCTT AATCAGTGAG GCACCTATCT CAGCGATCTG TCTATTTCGT
     5161 TCATCCATAG TTGCCTGACT CCCCGTCGTG TAGATAACTA CGATACGGGA GGGCTTACCA
     5221 TCTGGCCCCA GTGCTGCAAT GATACCGCGA GAACCACGCT CACCGGCTCC AGATTTATCA
     5281 GCAATAAACC AGCCAGCCGG AAGGGCCGAG CGCAGAAGTG GTCCTGCAAC TTTATCCGCC
     5341 TCCATCCAGT CTATTAATTG TTGCCGGGAA GCTAGAGTAA GTAGTTCGCC AGTTAATAGT
     5401 TTGCGCAACG TTGTTGCCAT TGCTACAGGC ATCGTGGTGT CACGCTCGTC GTTTGGTATG
     5461 GCTTCATTCA GCTCCGGTTC CCAACGATCA AGGCGAGTTA CATGATCCCC CATGTTGTGC
     5521 AAAAAAGCGG TTAGCTCCTT CGGTCCTCCG ATCGTTGTCA GAAGTAAGTT GGCCGCAGTG
     5581 TTATCACTCA TGGTTATGGC AGCACTGCAT AATTCTCTTA CTGTCATGCC ATCCGTAAGA
     5641 TGCTTTTCTG TGACTGGTGA GTACTCAACC AAGTCATTCT GAGAATAGTG TATGCGGCGA
     5701 CCGAGTTGCT CTTGCCCGGC GTCAATACGG GATAATACCG CGCCACATAG CAGAACTTTA
     5761 AAAGTGCTCA TCATTGGAAA ACGTTCTTCG GGGCGAAAAC TCTCAAGGAT CTTACCGCTG
     5821 TTGAGATCCA GTTCGATGTA ACCCACTCGT GCACCCAACT GATCTTCAGC ATCTTTTACT
     5881 TTCACCAGCG TTTCTGGGTG AGCAAAAACA GGAAGGCAAA ATGCCGCAAA AAAGGGAATA
     5941 AGGGCGACAC GGAAATGTTG AATACTCATA CTCTTCCTTT TTCAATATTA TTGAAGCATT
     6001 TATCAAGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA
     6061 ATAGGGGTTC CGCGC
//