LOCUS       pEPQD1CB0867_(P3_4X_opa 6524 bp ds-DNA     circular     22-DEC-2020
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    complement(27..177)
                     /label="LB"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    complement(96..120)
                     /label="LB T-DNA repeat"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    229..337
                     /label="OpattB"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    229..342
                     /label="MinSyn_068"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    260..283
                     /label="repeat seq"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    319..342
                     /label="repeat"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    347..350
                     /label="additional sites for pcr"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    351..459
                     /label="OpattB"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    382..405
                     /label="repeat seq"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    441..464
                     /label="repeat"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    464..512
                     /label="min 35S"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    520..540
                     /label="part of TMV 5'UTR"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    547..572
                     /label="part of TMV 5'UTR"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    593..659
                     /label="TMV omega"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    664..2106
                     /label="Cr7-DLGT (aka CrUGT8) (domesticated, 1 BsaI site removed) UGT709C2 AB733667.1 >ENA BAO01109 BAO01109.1 Catharanthus roseus (Madagascar periwinkle) UDP-glucose iridoid glucosyltransferase"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     Terminator      2114..2376
                     /label="Atu nos terminator, pICH41421"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    complement(2404..2529)
                     /label="RB"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    complement(2422..2446)
                     /label="RB T-DNA repeat"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     conflict        2511..2529
                     /label="partial overdrive"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     Origin          complement(2559..4040)
                     /label="RK2"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     CDS             complement(2602..3750)
                     /label="Plasmid replication initiator protein TrfA, P07676 (TRFA_ECOLX)"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    complement(4071..4685)
                     /label="replicon of pMB1"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     rep_origin      complement(4071..4690)
                     /label="pBR322\(from\pUC18)"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     primer_bind     complement(4166..4185)
                     /label="pBR322ori-F"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     CDS             complement(4845..5705)
                     /label="AmpR"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     promoter        complement(5706..5810)
                     /label="AmpR promoter"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     rep_origin      complement(join(5897..6524,1..4))
                     /label="oriV"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
ORIGIN
        1 CGTCGCAAAG GAGATCCTGA TCTGACTGAT GGGCTGCCTG TATCGAGTGG TGATTTTGTG
       61 CCGAGCTGCC GGTCGGGGAG CTGTTGGCTG GCTGGTGGCA GGATATATTG TGGTGTAAAC
      121 AAATTGACGC TTAGACAACT TAATAACACA TTGCGGACGT TTTTAATGTA CTGGGGTGGA
      181 TGCAGTGGGC CCCACTCTGT GAAGACAAAC TAGAATTCGA GCTCGGAGcg gtgcgggtgc
      241 cagggcgtgc ccttgggctc cccgggcgcg tactccactc cccgagccgg tgcgggtgcc
      301 agggcgtgcc cttgggctcc ccgggcgcgt actccactcc ccCCATggtg cggtgcgggt
      361 gccagggcgt gcccttgggc tccccgggcg cgtactccac tccccgagcc ggtgcgggtg
      421 ccagggcgtg cccttgggct ccccgggcgc gtactccact ccccttcgca agacccttcc
      481 tctatataag gaagttcatt tcatttggag aggactccgg tatttttaca acaattacca
      541 caacaaaaca aacaacaaac aacattacaa tttactattc tagtcgaaTA CTGTATTTTT
      601 ACAACAATTA CCAACAACAA CAAACAACAA ACAACATTAC AATTACTATT TACAATTACA
      661 ATGGGTTCTC AAGAAACAAA TTTGGCTCCT CATGTTCTCA TATTCCCTTT GCCAATTCAA
      721 GGCCATGTGA ACTCCATGCT CAGACTAGCA GAACTTCTCT GTCTAGCTGA ATTAGATATC
      781 ACTTTTATTG TTTCAGAGTT CAGCCACAGT CGCTTAATTA AGCATACAAA TGTTGCCTCC
      841 CGTTTTGCCC GTTACCCAGG ATTTCAGTTC CAGCCCATAT CCGATGGCCT TCCCGATGAT
      901 CACCCCAGAG CAGGCGAAAG AGTCATGGAT ATTTTGCCTT CAACTAAAAA TGTAACGGGA
      961 CCCCTGTTTA AGCAGATGAT GGTTGAGAAT AAGTGCTTTT CTTCTGCTAC TCGAAGGCCG
     1021 ATTACTTGCA TAATCGCTGA TGGGGTTTTG AGCTTTGCTG GGGATTTCGC TCAAGAAAAA
     1081 GGAATCCCTC TTATTTACTT CAGAACTGTT TCTGCTTGTT CTTTCTGGGC TTGTTTTTGT
     1141 ATGCCTGAAC TAATTGAATC TGGTGACATT CCGATTAAAG GAAATGGTAT GGATCTGATA
     1201 GTGAAAAGCG TGCCGGGGAT GGAAACTTTT CTCAGGCGAC GAGATCTGCC GGGGTTTTGC
     1261 CGAGTCAATG ACATTAATGA ACCGAAACTC CAGATCCTGA AAACAGAGAC AAGACAGACA
     1321 ACGAGAGCCC AAGCGGCTAT TTTGAACACT TTTGAGGATT TGGAAGGACC AATTCTATCT
     1381 CAAATTCGCA AACACATGCC AAGACTTTTC ACAATTGGAC CAAGTCATTC CCACCTAACA
     1441 TCTAGACTTG AAACCAAGAA TATCAAGACT TTAATTTCCT CCGGTAGTTT CTGGGAAGAA
     1501 GATCGAAGCT GTGTGGATTG GCTTGATGCA CAGCCACCAA GATCTGTTTT ATATGTAAGT
     1561 TTTGGAAGTA TTACAGTTGT AACAAGAGAT CAACTCCTGG AATTCTGGTA TGGTCTTGTT
     1621 AATAGTGGAC AACGGTTCTT GTGGGTAATG AGGCCAGATT CAATTATGGG CAAAGATGGG
     1681 CAAAGCCAGA TTCCTGCAGA TCTTGAAGAG GGCACAAAAG CCAGAGGTTA TATGGTGGGA
     1741 TGGGCACCTC AAGAAGAGGT CTTGAATCAC CCTGCCATTG GAGGATTTCT GACTCACAGT
     1801 GGGTGGAATT CAACTTTAGA GAGTATTGTG GCTGGTGTCC CAATGATATG TTGGCCATAC
     1861 TTTGCGGACC AAATGATAAA CAGCAGATTT GTGAGTGAAA TTTGGAAGAT TGGATTGGAT
     1921 ATGAAAGATA CATGTGATAG AGAGACAATT GTGAAGATGG TTAGGGAGTT GATGGAGATT
     1981 AGGAAAGATG AGTTCTTGCA AAGGGCTGAT CATATGGCTA AATTGGCAAA AGAGGCTGTT
     2041 AGTGAAGGCG GATCCTCATA TTCTAACTTG GATGGCCTCG TTGATTACAT AAAATCACTG
     2101 ATTATTTGAG CTTGTCAAGC AGATCGTTCA AACATTTGGC AATAAAGTTT CTTAAGATTG
     2161 AATCCTGTTG CCGGTCTTGC GATGATTATC ATATAATTTC TGTTGAATTA CGTTAAGCAT
     2221 GTAATAATTA ACATGTAATG CATGACGTTA TTTATGAGAT GGGTTTTTAT GATTAGAGTC
     2281 CCGCAATTAT ACATTTAATA CGCGATAGAA AACAAAATAT AGCGCGCAAA CTAGGATAAA
     2341 TTATCGCGCG CGGTGTCATC TATGTTACTA GATCGACGCT TTACTTGTCT TCTGCACGAA
     2401 GTGGTTTAAA CTATCAGTGT TTGACAGGAT ATATTGGCGG GTAAACCTAA GAGAAAAGAG
     2461 CGTTTATTAG AATAATCGGA TATTTAAAAG GGCGTGAAAA GGTTTATCCG TTCGTCCATT
     2521 TGTATGTGCA TGCCAACCAC AGGGTTCCCC AGATCAGGCG CTGGCTGCTG AACCCCCAGC
     2581 CGGAACTGAC CCCACAAGGC CCTAGCGTTT GCAATGCACC AGGTCATCAT TGACCCAGGC
     2641 GTGTTCCACC AGGCCGCTGC CTCGCAACTC TTCGCAGGCT TCGCCGACCT GCTCGCGCCA
     2701 CTTCTTCACG CGGGTGGAAT CCGATCCGCA CATGAGGCGG AAGGTTTCCA GCTTGAGCGG
     2761 GTACGGCTCC CGGTGCGAGC TGAAATAGTC GAACATCCGT CGGGCCGTCG GCGACAGCTT
     2821 GCGGTACTTC TCCCATATGA ATTTCGTGTA GTGGTCGCCA GCAAACAGCA CGACGATTTC
     2881 CTCGTCGATC AGGACCTGGC AACGGGACGT TTTCTTGCCA CGGTCCAGGA CGCGGAAGCG
     2941 GTGCAGCAGC GACACCGATT CCAGGTGCCC AACGCGGTCG GACGTGAAGC CCATCGCCGT
     3001 CGCCTGTAGG CGCGACAGGC ATTCCTCGGC CTTCGTGTAA TACCGGCCAT TGATCGACCA
     3061 GCCCAGGTCC TGGCAAAGCT CGTAGAACGT GAAGGTGATC GGCTCGCCGA TAGGGGTGCG
     3121 CTTCGCGTAC TCCAACACCT GCTGCCACAC CAGTTCGTCA TCGTCGGCCC GCAGCTCGAC
     3181 GCCGGTGTAG GTGATCTTCA CGTCCTTGTT GACGTGGAAA ATGACCTTGT TTTGCAGCGC
     3241 CTCGCGCGGG ATTTTCTTGT TGCGCGTGGT GAACAGGGCA GAGCGGGCCG TGTCGTTTGG
     3301 CATCGCTCGC ATCGTGTCCG GCCACGGCGC AATATCGAAC AAGGAAAGCT GCATTTCCTT
     3361 GATCTGCTGC TTCGTGTGTT TCAGCAACGC GGCCTGCTTG GCCTCGCTGA CCTGTTTTGC
     3421 CAGGTCCTCG CCGGCGGTTT TTCGCTTCTT GGTCGTCATA GTTCCTCGCG TGTCGATGGT
     3481 CATCGACTTC GCCAAACCTG CCGCCTCCTG TTCAAGACGA CGCGAACGCT CCACGGCGGC
     3541 CGATGGCGCG GGCAGGGCAG GGGGAGCCAG TTGCACGCTG TCGCGCTCGA TCTTGGCCGT
     3601 AGCTTGCTGG ACCATCGAGC CGACGGACTG GAAGGTTTCG CGGGGCGCAC GCATGACGGT
     3661 GCGGCTTGCG ATGGTTTCGG CATCCTCGGC GGAAAACCCC GCGTCGATCA GTTCTTGCCT
     3721 GTATGCCTTC CGGTCAAACG TCCGATTCAT TCACCCTCCT TGCGGGATTG CCCCGACTCA
     3781 CGCCGGGGCA ATGTGCCCTT ATTCCTGATT TGACCCGCCT GGTGCCTTGG TGTCCAGATA
     3841 ATCCACCTTA TCGGCAATGA AGTCGGTCCC GTAGACCGTC TGGCCGTCCT TCTCGTACTT
     3901 GGTATTCCGA ATCTTGCCCT GCACGAATAC CAGCGACCCC TTGCCCAAAT ACTTGCCGTG
     3961 GGCCTCGGCC TGAGAGCCAA AACACTTGAT GCGGAAGAAG TCGGTGCGCT CCTGCTTGTC
     4021 GCCGGCATCG TTGCGCCACA TCTAGGATCT GCCAGGAACC GTAAAAAGGC CGCGTTGCTG
     4081 GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG
     4141 AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC
     4201 GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG
     4261 GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT
     4321 CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC
     4381 GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC
     4441 ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG
     4501 TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA
     4561 GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC
     4621 GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT
     4681 CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT
     4741 TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT
     4801 TTTAAATCAA TCTAAAGTAT ATATGAGTAA ACTTGGTCTG ACAGTTACCA ATGCTTAATC
     4861 AGTGAGGCAC CTATCTCAGC GATCTGTCTA TTTCGTTCAT CCATAGTTGC CTGACTCCCC
     4921 GTCGTGTAGA TAACTACGAT ACGGGAGGGC TTACCATCTG GCCCCAGTGC TGCAATGATA
     4981 CCGCGAGAAC CACGCTCACC GGCTCCAGAT TTATCAGCAA TAAACCAGCC AGCCGGAAGG
     5041 GCCGAGCGCA GAAGTGGTCC TGCAACTTTA TCCGCCTCCA TCCAGTCTAT TAATTGTTGC
     5101 CGGGAAGCTA GAGTAAGTAG TTCGCCAGTT AATAGTTTGC GCAACGTTGT TGCCATTGCT
     5161 ACAGGCATCG TGGTGTCACG CTCGTCGTTT GGTATGGCTT CATTCAGCTC CGGTTCCCAA
     5221 CGATCAAGGC GAGTTACATG ATCCCCCATG TTGTGCAAAA AAGCGGTTAG CTCCTTCGGT
     5281 CCTCCGATCG TTGTCAGAAG TAAGTTGGCC GCAGTGTTAT CACTCATGGT TATGGCAGCA
     5341 CTGCATAATT CTCTTACTGT CATGCCATCC GTAAGATGCT TTTCTGTGAC TGGTGAGTAC
     5401 TCAACCAAGT CATTCTGAGA ATAGTGTATG CGGCGACCGA GTTGCTCTTG CCCGGCGTCA
     5461 ATACGGGATA ATACCGCGCC ACATAGCAGA ACTTTAAAAG TGCTCATCAT TGGAAAACGT
     5521 TCTTCGGGGC GAAAACTCTC AAGGATCTTA CCGCTGTTGA GATCCAGTTC GATGTAACCC
     5581 ACTCGTGCAC CCAACTGATC TTCAGCATCT TTTACTTTCA CCAGCGTTTC TGGGTGAGCA
     5641 AAAACAGGAA GGCAAAATGC CGCAAAAAAG GGAATAAGGG CGACACGGAA ATGTTGAATA
     5701 CTCATACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AAGGTTATTG TCTCATGAGC
     5761 GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACGAATTGG
     5821 CCAGCGCTGC CATTTTTGGG GTGAGGCCGT TCGCGGCCGA GGGGCGCAGC CCCTGGGGGG
     5881 ATGGGAGGCC CGCGTTAGCG GGCCGGGAGG GTTCGAGAAG GGGGGGCACC CCCCTTCGGC
     5941 GTGCGCGGTC ACGCGCACAG GGCGCAGCCC TGGTTAAAAA CAAGGTTTAT AAATATTGGT
     6001 TTAAAAGCAG GTTAAAAGAC AGGTTAGCGG TGGCCGAAAA ACGGGCGGAA ACCCTTGCAA
     6061 ATGCTGGATT TTCTGCCTGT GGACAGCCCC TCAAATGTCA ATAGGTGCGC CCCTCATCTG
     6121 TCAGCACTCT GCCCCTCAAG TGTCAAGGAT CGCGCCCCTC ATCTGTCAGT AGTCGCGCCC
     6181 CTCAAGTGTC AATACCGCAG GGCACTTATC CCCAGGCTTG TCCACATCAT CTGTGGGAAA
     6241 CTCGCGTAAA ATCAGGCGTT TTCGCCGATT TGCGAGGCTG GCCAGCTCCA CGTCGCCGGC
     6301 CGAAATCGAG CCTGCCCCTC ATCTGTCAAC GCCGCGCCGG GTGAGTCGGC CCCTCAAGTG
     6361 TCAACGTCCG CCCCTCATCT GTCAGTGAGG GCCAAGTTTT CCGCGAGGTA TCCACAACGC
     6421 CGGCGGCCGC GGTGTCTCGC ACACGGCTTC GACGGCGTTT CTGGCGCGTT TGCAGGGCCA
     6481 TAGACGGCCG CCAGCCCAGC GGCGAGGGCA ACCAGCCCGG TGAG
//