LOCUS       pEPQD1CB0871_(P2_4X_opa 6659 bp ds-DNA     circular     22-DEC-2020
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    complement(26..176)
                     /label="LB"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    complement(95..119)
                     /label="LB T-DNA repeat"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    228..336
                     /label="OpattB"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    228..341
                     /label="MinSyn_068"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    259..282
                     /label="repeat seq"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    318..341
                     /label="repeat"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    346..349
                     /label="additional sites for pcr"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    350..458
                     /label="OpattB"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    381..404
                     /label="repeat seq"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    440..463
                     /label="repeat"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    463..511
                     /label="min 35S"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    519..539
                     /label="part of TMV 5'UTR"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    546..571
                     /label="part of TMV 5'UTR"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    592..658
                     /label="TMV omega"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    663..2240
                     /label="name depends on ref: SLS1 Brown et al. PNAS 112(11) 3205-3210 (2015), SLS2 de Bernonville et al. BMC Genomics 16(1) 619 (2015)"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_feature    663..2240
                     /label="CrSLS secologanin synthase, Catharanthus roseus KF309242.1 (domesticated, 1 SapI site removed))"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     Terminator      2248..2510
                     /label="Atu nos terminator, pICH41421"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    complement(2538..2663)
                     /label="RB"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    complement(2556..2580)
                     /label="RB T-DNA repeat"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     conflict        2645..2663
                     /label="partial overdrive"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     Origin          complement(2693..4174)
                     /label="RK2"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     CDS             complement(2736..3884)
                     /label="Plasmid replication initiator protein TrfA, P07676 (TRFA_ECOLX)"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     primer_bind     complement(4300..4319)
                     /label="pBR322ori-F"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     CDS             complement(4979..5839)
                     /label="AmpR"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     rep_origin      complement(join(6031..6659,1..3))
                     /label="oriV"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
ORIGIN
        1 GTCGCAAAGG AGATCCTGAT CTGACTGATG GGCTGCCTGT ATCGAGTGGT GATTTTGTGC
       61 CGAGCTGCCG GTCGGGGAGC TGTTGGCTGG CTGGTGGCAG GATATATTGT GGTGTAAACA
      121 AATTGACGCT TAGACAACTT AATAACACAT TGCGGACGTT TTTAATGTAC TGGGGTGGAT
      181 GCAGTGGGCC CCACTCTGTG AAGACAAGCA AGAATTCAAG CTTGGAGcgg tgcgggtgcc
      241 agggcgtgcc cttgggctcc ccgggcgcgt actccactcc ccgagccggt gcgggtgcca
      301 gggcgtgccc ttgggctccc cgggcgcgta ctccactccc cCCATggtgc ggtgcgggtg
      361 ccagggcgtg cccttgggct ccccgggcgc gtactccact ccccgagccg gtgcgggtgc
      421 cagggcgtgc ccttgggctc cccgggcgcg tactccactc cccttcgcaa gacccttcct
      481 ctatataagg aagttcattt catttggaga ggactccggt atttttacaa caattaccac
      541 aacaaaacaa acaacaaaca acattacaat ttactattct agtcgaaTAC TGTATTTTTA
      601 CAACAATTAC CAACAACAAC AAACAACAAA CAACATTACA ATTACTATTT ACAATTACAA
      661 TGGAGATGGA TATGGATATC ATTAGAAAGG CAATTGCTGC CACTATCTTT GCATTGGTTA
      721 TGGCTTGGGC GTGGAGAGTG TTGGATTGGG CATGGTTTAC TCCTAAGAGG ATCGAGAAAC
      781 GTCTAAGGCA GCAAGGTTTT AGAGGAAATC CTTATAGATT CTTGGTTGGA GATGTTAAGG
      841 AGAGTGGAAA AATGCATCAA GAAGCCTTGT CTAACCCCAT GGAGTTCGAC AACGATATTG
      901 TTCCTCGCCT CATGCCACAT ATTAACCACA CCATAAAAAC TTACGGTAGG AATTCATTTA
      961 CATGGATGGG AAGGATTCCA AGGATTCATG TTATGGAACC TGAACTTATT AAGGAAGTAT
     1021 TGACCCACTC CAGCAAATAC CAAAAGAACT TTGATGTTCA CAATCCCCTT GTTAAGTTCC
     1081 TTCTCACTGG AGTTGGAAGC TTTGAAGGGG CAAAATGGTC CAAACACAGA AGAATCATTT
     1141 CCCCTGCCTT CACTCTTGAG AAACTAAAGT CAATGTTGCC GGCTTTTGCC ATATGCTATC
     1201 ATGACATGTT GACGAAATGG GAGAAATTAG CAGAAAAAGA AGGATCCCAT GAAGTTGATA
     1261 TCTTCCCCAC ATTTGATGTT TTGACAAGTG ATGTAATTTC AAAGGTTGCA TTTGGTAGCA
     1321 CATACGATGA AGGAGGCAAA ATCTTCAGAC TATTGAAAGA ACTCATGGAT CTCACAATTG
     1381 ATTGCATGAG AGATGTCTAC ATCCCAGGGT GGAGCTACTT GCCAACCAAG AGGAACAAGA
     1441 GGATGAAAGA AATTAACAAA GAAATCACAG ATATGCTAAG GTTCATCATC AACAAGAGAA
     1501 TGAAGGCTTT GAAGGCTGGA GAACCAGGTG AGGATGACTT GTTGGGAGTC CTGTTGGAAT
     1561 CAAACATTCA AGAAATTCAG AAACAAGGAA ACAGGAAGGA TGGTGGAATG ACAATCAACG
     1621 ATGTGATTGA GGAATGCAAA TTGTTCTACT TTGCTGGTCA AGAAACCACC GGAGTTTTGC
     1681 TCACATGGAC CACCATTTTA TTGAGCAAGC ATCCCGAGTG GCAAGAGCGA GCTAGGGAAG
     1741 AAGTTCTCCA AGCCTTTGGC AAGAACAAGC CTGAATTTGA ACGCTTGAAT CATCTTAAAT
     1801 ATGTTTCTAT GATCTTATAC GAGGTTCTAA GGTTGTACCC GCCAGTTATT GATCTGACCA
     1861 AGATTATTCA CGAGGACACA AAGTTAGGGC CGTACACAAT CCCTGCAGGA ACACAAGTAA
     1921 TGTTGCCGAC AGTAATGCTT CATAGAGAGA AGTCCATTTG GGGAGAAGAT GCAATGGAAT
     1981 TCAACCCAAT GAGATTTGCT GATGGAGTTG CCAATGCCAC CAAGAACAAT GTCACATATT
     2041 TGCCATTCAG TTGGGGACCT AGAGTTTGCC TCGGCCAAAA CTTTGCACTT CTTCAAGCAA
     2101 AATTAGGGTT GGCAATGATT TTACAACGCT TCAAATTTGA TGTAGCTCCA TCCTATGTTC
     2161 ATGCTCCCTT TACCATTCTT ACAGTTCAAC CTCAGTTTGG TTCTCATGTT ATCTACAAGA
     2221 AGCTTGAGAG ACAGAATTTT TAAGCTTGTC AAGCAGATCG TTCAAACATT TGGCAATAAA
     2281 GTTTCTTAAG ATTGAATCCT GTTGCCGGTC TTGCGATGAT TATCATATAA TTTCTGTTGA
     2341 ATTACGTTAA GCATGTAATA ATTAACATGT AATGCATGAC GTTATTTATG AGATGGGTTT
     2401 TTATGATTAG AGTCCCGCAA TTATACATTT AATACGCGAT AGAAAACAAA ATATAGCGCG
     2461 CAAACTAGGA TAAATTATCG CGCGCGGTGT CATCTATGTT ACTAGATCGA CGCTACTATT
     2521 GTCTTCTGCA CGAAGTGGTT TAAACTATCA GTGTTTGACA GGATATATTG GCGGGTAAAC
     2581 CTAAGAGAAA AGAGCGTTTA TTAGAATAAT CGGATATTTA AAAGGGCGTG AAAAGGTTTA
     2641 TCCGTTCGTC CATTTGTATG TGCATGCCAA CCACAGGGTT CCCCAGATCA GGCGCTGGCT
     2701 GCTGAACCCC CAGCCGGAAC TGACCCCACA AGGCCCTAGC GTTTGCAATG CACCAGGTCA
     2761 TCATTGACCC AGGCGTGTTC CACCAGGCCG CTGCCTCGCA ACTCTTCGCA GGCTTCGCCG
     2821 ACCTGCTCGC GCCACTTCTT CACGCGGGTG GAATCCGATC CGCACATGAG GCGGAAGGTT
     2881 TCCAGCTTGA GCGGGTACGG CTCCCGGTGC GAGCTGAAAT AGTCGAACAT CCGTCGGGCC
     2941 GTCGGCGACA GCTTGCGGTA CTTCTCCCAT ATGAATTTCG TGTAGTGGTC GCCAGCAAAC
     3001 AGCACGACGA TTTCCTCGTC GATCAGGACC TGGCAACGGG ACGTTTTCTT GCCACGGTCC
     3061 AGGACGCGGA AGCGGTGCAG CAGCGACACC GATTCCAGGT GCCCAACGCG GTCGGACGTG
     3121 AAGCCCATCG CCGTCGCCTG TAGGCGCGAC AGGCATTCCT CGGCCTTCGT GTAATACCGG
     3181 CCATTGATCG ACCAGCCCAG GTCCTGGCAA AGCTCGTAGA ACGTGAAGGT GATCGGCTCG
     3241 CCGATAGGGG TGCGCTTCGC GTACTCCAAC ACCTGCTGCC ACACCAGTTC GTCATCGTCG
     3301 GCCCGCAGCT CGACGCCGGT GTAGGTGATC TTCACGTCCT TGTTGACGTG GAAAATGACC
     3361 TTGTTTTGCA GCGCCTCGCG CGGGATTTTC TTGTTGCGCG TGGTGAACAG GGCAGAGCGG
     3421 GCCGTGTCGT TTGGCATCGC TCGCATCGTG TCCGGCCACG GCGCAATATC GAACAAGGAA
     3481 AGCTGCATTT CCTTGATCTG CTGCTTCGTG TGTTTCAGCA ACGCGGCCTG CTTGGCCTCG
     3541 CTGACCTGTT TTGCCAGGTC CTCGCCGGCG GTTTTTCGCT TCTTGGTCGT CATAGTTCCT
     3601 CGCGTGTCGA TGGTCATCGA CTTCGCCAAA CCTGCCGCCT CCTGTTCAAG ACGACGCGAA
     3661 CGCTCCACGG CGGCCGATGG CGCGGGCAGG GCAGGGGGAG CCAGTTGCAC GCTGTCGCGC
     3721 TCGATCTTGG CCGTAGCTTG CTGGACCATC GAGCCGACGG ACTGGAAGGT TTCGCGGGGC
     3781 GCACGCATGA CGGTGCGGCT TGCGATGGTT TCGGCATCCT CGGCGGAAAA CCCCGCGTCG
     3841 ATCAGTTCTT GCCTGTATGC CTTCCGGTCA AACGTCCGAT TCATTCACCC TCCTTGCGGG
     3901 ATTGCCCCGA CTCACGCCGG GGCAATGTGC CCTTATTCCT GATTTGACCC GCCTGGTGCC
     3961 TTGGTGTCCA GATAATCCAC CTTATCGGCA ATGAAGTCGG TCCCGTAGAC CGTCTGGCCG
     4021 TCCTTCTCGT ACTTGGTATT CCGAATCTTG CCCTGCACGA ATACCAGCGA CCCCTTGCCC
     4081 AAATACTTGC CGTGGGCCTC GGCCTGAGAG CCAAAACACT TGATGCGGAA GAAGTCGGTG
     4141 CGCTCCTGCT TGTCGCCGGC ATCGTTGCGC CACATCTAGG ATCTGCCAGG AACCGTAAAA
     4201 AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC
     4261 GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC
     4321 CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG
     4381 CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT
     4441 CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC
     4501 GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC
     4561 CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG
     4621 AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG GACAGTATTT GGTATCTGCG
     4681 CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA
     4741 CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG
     4801 GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT
     4861 CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA
     4921 ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA GTAAACTTGG TCTGACAGTT
     4981 ACCAATGCTT AATCAGTGAG GCACCTATCT CAGCGATCTG TCTATTTCGT TCATCCATAG
     5041 TTGCCTGACT CCCCGTCGTG TAGATAACTA CGATACGGGA GGGCTTACCA TCTGGCCCCA
     5101 GTGCTGCAAT GATACCGCGA GAACCACGCT CACCGGCTCC AGATTTATCA GCAATAAACC
     5161 AGCCAGCCGG AAGGGCCGAG CGCAGAAGTG GTCCTGCAAC TTTATCCGCC TCCATCCAGT
     5221 CTATTAATTG TTGCCGGGAA GCTAGAGTAA GTAGTTCGCC AGTTAATAGT TTGCGCAACG
     5281 TTGTTGCCAT TGCTACAGGC ATCGTGGTGT CACGCTCGTC GTTTGGTATG GCTTCATTCA
     5341 GCTCCGGTTC CCAACGATCA AGGCGAGTTA CATGATCCCC CATGTTGTGC AAAAAAGCGG
     5401 TTAGCTCCTT CGGTCCTCCG ATCGTTGTCA GAAGTAAGTT GGCCGCAGTG TTATCACTCA
     5461 TGGTTATGGC AGCACTGCAT AATTCTCTTA CTGTCATGCC ATCCGTAAGA TGCTTTTCTG
     5521 TGACTGGTGA GTACTCAACC AAGTCATTCT GAGAATAGTG TATGCGGCGA CCGAGTTGCT
     5581 CTTGCCCGGC GTCAATACGG GATAATACCG CGCCACATAG CAGAACTTTA AAAGTGCTCA
     5641 TCATTGGAAA ACGTTCTTCG GGGCGAAAAC TCTCAAGGAT CTTACCGCTG TTGAGATCCA
     5701 GTTCGATGTA ACCCACTCGT GCACCCAACT GATCTTCAGC ATCTTTTACT TTCACCAGCG
     5761 TTTCTGGGTG AGCAAAAACA GGAAGGCAAA ATGCCGCAAA AAAGGGAATA AGGGCGACAC
     5821 GGAAATGTTG AATACTCATA CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT
     5881 ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC
     5941 CGCGCACGAA TTGGCCAGCG CTGCCATTTT TGGGGTGAGG CCGTTCGCGG CCGAGGGGCG
     6001 CAGCCCCTGG GGGGATGGGA GGCCCGCGTT AGCGGGCCGG GAGGGTTCGA GAAGGGGGGG
     6061 CACCCCCCTT CGGCGTGCGC GGTCACGCGC ACAGGGCGCA GCCCTGGTTA AAAACAAGGT
     6121 TTATAAATAT TGGTTTAAAA GCAGGTTAAA AGACAGGTTA GCGGTGGCCG AAAAACGGGC
     6181 GGAAACCCTT GCAAATGCTG GATTTTCTGC CTGTGGACAG CCCCTCAAAT GTCAATAGGT
     6241 GCGCCCCTCA TCTGTCAGCA CTCTGCCCCT CAAGTGTCAA GGATCGCGCC CCTCATCTGT
     6301 CAGTAGTCGC GCCCCTCAAG TGTCAATACC GCAGGGCACT TATCCCCAGG CTTGTCCACA
     6361 TCATCTGTGG GAAACTCGCG TAAAATCAGG CGTTTTCGCC GATTTGCGAG GCTGGCCAGC
     6421 TCCACGTCGC CGGCCGAAAT CGAGCCTGCC CCTCATCTGT CAACGCCGCG CCGGGTGAGT
     6481 CGGCCCCTCA AGTGTCAACG TCCGCCCCTC ATCTGTCAGT GAGGGCCAAG TTTTCCGCGA
     6541 GGTATCCACA ACGCCGGCGG CCGCGGTGTC TCGCACACGG CTTCGACGGC GTTTCTGGCG
     6601 CGTTTGCAGG GCCATAGACG GCCGCCAGCC CAGCGGCGAG GGCAACCAGC CCGGTGAGC
//