LOCUS       Exported                9246 bp DNA     circular SYN 09-AUG-2021
DEFINITION  Plasmid for chromosomal tagging of Abp1 with six copies of iGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9246)
  AUTHORS   Glick Lab
  TITLE     Direct Submission
  JOURNAL   Exported Sep 20, 2021 from SnapGene 5.3.2
            https://www.snapgene.com
COMMENT     Linearize with XbaI for pop-in integration at ABP1, then select for 
            pop-outs on 5-FOA.
FEATURES             Location/Qualifiers
     source          1..9246
                     /organism="synthetic DNA construct"
                     /lab_host="Saccharomyces cerevisiae"
                     /mol_type="other DNA"
     CDS             244..834
                     /codon_start=1
                     /label=ABP1
                     /translation="PQEPVKAEEAEQPKTDYKKIGNPLPGMHIEADNEEEPEENDDDWD
                     DDEDEAAQPPLPSRNVASGAPVQKEEPEQEEIAPSLPSRNSIPAPKQEEAPEQAPEEEI
                     EEEAEEAAPQLPSRSSAAPPPPPRRATPEKKPKENPWATAEYDYDAAEDNELTFVENDK
                     IINIEFVDDDWWLGELEKDGSKGLFPSNYVSLGN"
     CDS             835..879
                     /codon_start=1
                     /label=linker
                     /translation="TDPGSSGSSGSSGAS"
     CDS             880..1594
                     /codon_start=1
                     /label=iGFP
                     /translation="MDSTESLFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKA
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLKE
                     FVTAAGITHGSSGSSG"
     CDS             1600..2314
                     /codon_start=1
                     /label=iGFP
                     /translation="MDSTESLFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKA
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLKE
                     FVTAAGITHGSSGSSG"
     CDS             2320..3034
                     /codon_start=1
                     /label=iGFP
                     /translation="MDSTESLFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKA
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLKE
                     FVTAAGITHGSSGSSG"
     CDS             3040..3754
                     /codon_start=1
                     /label=iGFP
                     /translation="MDSTESLFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKA
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLKE
                     FVTAAGITHGSSGSSG"
     CDS             3760..4474
                     /codon_start=1
                     /label=iGFP
                     /translation="MDSTESLFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKA
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLKE
                     FVTAAGITHGSSGSSG"
     CDS             4480..5194
                     /codon_start=1
                     /label=iGFP
                     /translation="MDSTESLFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKA
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLKE
                     FVTAAGITHGSSGSSG"
     misc_feature    5206..5721
                     /label=3' ABP1
     CDS             complement(6223..7026)
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     promoter        complement(7027..7247)
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     promoter        7340..7444
                     /gene="bla"
                     /label=AmpR promoter
     CDS             7445..8305
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      8476..9064
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number colE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
       61 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
      121 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
      181 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg ccaagcttgc
      241 atgccacaag aacctgtgaa agctgaagaa gccgagcagc ctaagactga ttacaagaag
      301 atcggcaacc cattacccgg tatgcacatt gaagcggata atgaggaaga accagaagag
      361 aatgatgatg actgggatga tgatgaagac gaggctgctc aacctccttt gccttcgagg
      421 aatgttgcgt caggagcacc agtgcaaaaa gaagagcctg aacaagaaga gatcgcccca
      481 agcttacctt ctagaaactc gatcccagct ccaaaacaag aagaagcacc tgaacaagca
      541 cctgaagaag aaattgaaga agaagctgag gaagccgctc cacagctgcc atcaagaagc
      601 tctgcagctc ctcctccgcc tccaagacga gcaactccag agaaaaagcc aaaggaaaat
      661 ccttgggcca cagcagaata tgattacgat gctgcagaag ataacgaact gacctttgtg
      721 gaaaatgaca agattatcaa tattgaattt gtcgacgatg actggtggct aggggaacta
      781 gagaaagacg gctcaaaagg tctcttcccc agcaattatg tgtctttggg caacacggat
      841 ccaggaagtt cggggtccag cggttcttca ggcgctagca tggatagcac tgagagcctt
      901 ttcactggag ttgtcccaat tcttgttgaa ttagatggtg atgttaatgg gcacaaattt
      961 tctgtcagtg gagagggtga aggtgatgca acatacggaa aacttaccct taaatttatt
     1021 tgcactactg gaaaactacc tgttccttgg ccaacacttg tcactacttt aacttatggt
     1081 gttcagtgct tttcaagata cccggatcat atgaaacagc atgacttttt caagagtgcc
     1141 atgcccgaag gttatgtaca ggaaagaact atatttttca aagatgacgg gaactacaag
     1201 acacgtgctg aagtcaagtt tgaaggtgat acccttgtta atagaatcga gttaaaaggt
     1261 attgatttta aagaagatgg aaacattctt gggcacaaat tggaatacaa ctataactca
     1321 cacaatgtat acatcatggc agacaaacaa aagaatggaa tcaaagctaa cttcaaaatt
     1381 agacacaaca ttgaagatgg aagcgttcaa ctagcagacc attatcaaca aaatactcca
     1441 attggcgatg gccctgtcct tttaccagac aaccattacc tgtccacaca atctgccctt
     1501 tcgaaagatc ccaacgaaaa gagagaccac atggtcctta aagagtttgt aacagctgct
     1561 gggattacac atggcagctc cggctccagc gggtctagca tggatagcac tgagagcctt
     1621 ttcactggag ttgtcccaat tcttgttgaa ttagatggtg atgttaatgg gcacaaattt
     1681 tctgtcagtg gagagggtga aggtgatgca acatacggaa aacttaccct taaatttatt
     1741 tgcactactg gaaaactacc tgttccttgg ccaacacttg tcactacttt aacttatggt
     1801 gttcagtgct tttcaagata cccggatcat atgaaacagc atgacttttt caagagtgcc
     1861 atgcccgaag gttatgtaca ggaaagaact atatttttca aagatgacgg gaactacaag
     1921 acacgtgctg aagtcaagtt tgaaggtgat acccttgtta atagaatcga gttaaaaggt
     1981 attgatttta aagaagatgg aaacattctt gggcacaaat tggaatacaa ctataactca
     2041 cacaatgtat acatcatggc agacaaacaa aagaatggaa tcaaagctaa cttcaaaatt
     2101 agacacaaca ttgaagatgg aagcgttcaa ctagcagacc attatcaaca aaatactcca
     2161 attggcgatg gccctgtcct tttaccagac aaccattacc tgtccacaca atctgccctt
     2221 tcgaaagatc ccaacgaaaa gagagaccac atggtcctta aagagtttgt aacagctgct
     2281 gggattacac atggcagctc cggctccagc gggtctagca tggatagcac tgagagcctt
     2341 ttcactggag ttgtcccaat tcttgttgaa ttagatggtg atgttaatgg gcacaaattt
     2401 tctgtcagtg gagagggtga aggtgatgca acatacggaa aacttaccct taaatttatt
     2461 tgcactactg gaaaactacc tgttccttgg ccaacacttg tcactacttt aacttatggt
     2521 gttcagtgct tttcaagata cccggatcat atgaaacagc atgacttttt caagagtgcc
     2581 atgcccgaag gttatgtaca ggaaagaact atatttttca aagatgacgg gaactacaag
     2641 acacgtgctg aagtcaagtt tgaaggtgat acccttgtta atagaatcga gttaaaaggt
     2701 attgatttta aagaagatgg aaacattctt gggcacaaat tggaatacaa ctataactca
     2761 cacaatgtat acatcatggc agacaaacaa aagaatggaa tcaaagctaa cttcaaaatt
     2821 agacacaaca ttgaagatgg aagcgttcaa ctagcagacc attatcaaca aaatactcca
     2881 attggcgatg gccctgtcct tttaccagac aaccattacc tgtccacaca atctgccctt
     2941 tcgaaagatc ccaacgaaaa gagagaccac atggtcctta aagagtttgt aacagctgct
     3001 gggattacac atggcagctc cggctccagc gggtctagca tggatagcac tgagagcctt
     3061 ttcactggag ttgtcccaat tcttgttgaa ttagatggtg atgttaatgg gcacaaattt
     3121 tctgtcagtg gagagggtga aggtgatgca acatacggaa aacttaccct taaatttatt
     3181 tgcactactg gaaaactacc tgttccttgg ccaacacttg tcactacttt aacttatggt
     3241 gttcagtgct tttcaagata cccggatcat atgaaacagc atgacttttt caagagtgcc
     3301 atgcccgaag gttatgtaca ggaaagaact atatttttca aagatgacgg gaactacaag
     3361 acacgtgctg aagtcaagtt tgaaggtgat acccttgtta atagaatcga gttaaaaggt
     3421 attgatttta aagaagatgg aaacattctt gggcacaaat tggaatacaa ctataactca
     3481 cacaatgtat acatcatggc agacaaacaa aagaatggaa tcaaagctaa cttcaaaatt
     3541 agacacaaca ttgaagatgg aagcgttcaa ctagcagacc attatcaaca aaatactcca
     3601 attggcgatg gccctgtcct tttaccagac aaccattacc tgtccacaca atctgccctt
     3661 tcgaaagatc ccaacgaaaa gagagaccac atggtcctta aagagtttgt aacagctgct
     3721 gggattacac atggcagctc cggctccagc gggtctagca tggatagcac tgagagcctt
     3781 ttcactggag ttgtcccaat tcttgttgaa ttagatggtg atgttaatgg gcacaaattt
     3841 tctgtcagtg gagagggtga aggtgatgca acatacggaa aacttaccct taaatttatt
     3901 tgcactactg gaaaactacc tgttccttgg ccaacacttg tcactacttt aacttatggt
     3961 gttcagtgct tttcaagata cccggatcat atgaaacagc atgacttttt caagagtgcc
     4021 atgcccgaag gttatgtaca ggaaagaact atatttttca aagatgacgg gaactacaag
     4081 acacgtgctg aagtcaagtt tgaaggtgat acccttgtta atagaatcga gttaaaaggt
     4141 attgatttta aagaagatgg aaacattctt gggcacaaat tggaatacaa ctataactca
     4201 cacaatgtat acatcatggc agacaaacaa aagaatggaa tcaaagctaa cttcaaaatt
     4261 agacacaaca ttgaagatgg aagcgttcaa ctagcagacc attatcaaca aaatactcca
     4321 attggcgatg gccctgtcct tttaccagac aaccattacc tgtccacaca atctgccctt
     4381 tcgaaagatc ccaacgaaaa gagagaccac atggtcctta aagagtttgt aacagctgct
     4441 gggattacac atggcagctc cggctccagc gggtctagca tggatagcac tgagagcctt
     4501 ttcactggag ttgtcccaat tcttgttgaa ttagatggtg atgttaatgg gcacaaattt
     4561 tctgtcagtg gagagggtga aggtgatgca acatacggaa aacttaccct taaatttatt
     4621 tgcactactg gaaaactacc tgttccttgg ccaacacttg tcactacttt aacttatggt
     4681 gttcagtgct tttcaagata cccggatcat atgaaacagc atgacttttt caagagtgcc
     4741 atgcccgaag gttatgtaca ggaaagaact atatttttca aagatgacgg gaactacaag
     4801 acacgtgctg aagtcaagtt tgaaggtgat acccttgtta atagaatcga gttaaaaggt
     4861 attgatttta aagaagatgg aaacattctt gggcacaaat tggaatacaa ctataactca
     4921 cacaatgtat acatcatggc agacaaacaa aagaatggaa tcaaagctaa cttcaaaatt
     4981 agacacaaca ttgaagatgg aagcgttcaa ctagcagacc attatcaaca aaatactcca
     5041 attggcgatg gccctgtcct tttaccagac aaccattacc tgtccacaca atctgccctt
     5101 tcgaaagatc ccaacgaaaa gagagaccac atggtcctta aagagtttgt aacagctgct
     5161 gggattacac atggcagctc cggctccagc gggtaaagcg gccgcaatca cacgtcagcg
     5221 tcatgctatt atattattct tacgtaaaaa aatacttaca gagacgttaa agcttttgta
     5281 tagatacttt cacgagcctt ccctttcagt aaaatgaaca aattattttg ccttgtcctt
     5341 ctttgtttca agcgtttata tgactcctct acagtattat ttttcaacag acggtgtgcc
     5401 cgtacggcgt tgtcaaaacc tcgaacataa aacaacactg aaacaaactt attgcactcc
     5461 tgcatcgcat acagatctga tacttactac aaccataatg ctgttttaga atcgcatcag
     5521 gtgtttacca gaagtgtatg gtacatggtg ctttccttat gctcactatt tcggatttac
     5581 ttctagtcta tgtatagatt tgttagagac cagttttaag ctacaatgga acgttaagat
     5641 taagaaatgc atgaagagtc tcccagtaga tatataaagc tgaattaaat tcagtacaat
     5701 ggaagtttcc acgtctttct ggtaccgagc tcgaattcac tggccgtcgt tttacaacgt
     5761 cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc
     5821 gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc
     5881 ctgaatggcg aatggcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca
     5941 caccgcatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagccc
     6001 cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct
     6061 tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca
     6121 ccgaaacgcg cgagacgaaa gggcgggtaa taactgatat aattaaattg aagctctaat
     6181 ttgtgagttt agtatacatg catttactta taatacagtt ttttagtttt gctggccgca
     6241 tcttctcaaa tatgcttccc agcctgcttt tctgtaacgt tcaccctcta ccttagcatc
     6301 ccttcccttt gcaaatagtc ctcttccaac aataataatg tcagatcctg tagagaccac
     6361 atcatccacg gttctatact gttgacccaa tgcgtctccc ttgtcatcta aacccacacc
     6421 gggtgtcata atcaaccaat cgtaaccttc atctcttcca cccatgtctc tttgagcaat
     6481 aaagccgata acaaaatctt tgtcgctctt cgcaatgtca acagtaccct tagtatattc
     6541 tccagtagat agggagccct tgcatgacaa ttctgctaac atcaaaaggc ctctaggttc
     6601 ctttgttact tcttctgccg cctgcttcaa accgctaaca atacctgggc ccaccacacc
     6661 gtgtgcattc gtaatgtctg cccattctgc tattctgtat acacccgcag agtactgcaa
     6721 tttgactgta ttaccaatgt cagcaaattt tctgtcttcg aagagtaaaa aattgtactt
     6781 ggcggataat gcctttagcg gcttaactgt gccctccatg gaaaaatcag tcaagatatc
     6841 cacatgtgtt tttagtaaac aaattttggg acctaatgct tcaactaact ccagtaattc
     6901 cttggtggta cgaacatcca atgaagcaca caagtttgtt tgcttttcgt gcatgatatt
     6961 aaatagcttg gcagcaacag gactaggatg agtagcagca cgttccttat atgtagcttt
     7021 cgacatgatt tatcttcgtt tcctgcatgt ttttgttctg tgcagttggg ttaagaatac
     7081 tgggcaattt catgtttctt caacactaca tatgcgtata tataccaatc taagtctgtg
     7141 ctccttcctt cgttcttcct tctgttcgga gattaccgaa tcaaaaaaat ttcaaagaaa
     7201 ccgaaatcaa aaaaaagaat aaaaaaaaaa tgatgaattg aattgaaaag ctggcctcgt
     7261 gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg
     7321 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
     7381 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
     7441 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
     7501 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
     7561 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
     7621 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
     7681 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
     7741 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
     7801 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
     7861 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
     7921 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
     7981 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
     8041 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
     8101 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
     8161 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
     8221 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
     8281 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
     8341 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     8401 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     8461 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     8521 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     8581 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
     8641 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     8701 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     8761 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     8821 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     8881 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     8941 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     9001 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     9061 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     9121 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
     9181 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
     9241 ggaaga
//
