LOCUS       Exported                8505 bp DNA     circular SYN 21-SEP-2021
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8505)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 21, 2021 from SnapGene Viewer 5.3.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8505
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             complement(142..333)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /note="color: #993366"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(890..1972)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #993366"
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     promoter        complement(1973..2050)
                     /gene="lacI"
                     /label=lacI promoter
                     /note="color: #ffffff; direction: LEFT"
     terminator      2203..2289
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
                     /note="color: #ffffff; direction: RIGHT"
     terminator      2386..2472
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
                     /note="color: #ffffff; direction: RIGHT"
     terminator      2569..2655
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
                     /note="color: #ffffff; direction: RIGHT"
     terminator      2752..2838
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
                     /note="color: #ffffff; direction: RIGHT"
     terminator      2935..3021
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
                     /note="color: #ffffff; direction: RIGHT"
     promoter        3156..3174
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /note="color: #ffffff; direction: RIGHT"
     protein_bind    3175..3199
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #31849b"
     misc_feature    3240..3245
                     /label=NdeI Enzyme Active Site
                     /note="color: #ff0000"
     CDS             3243..3959
                     /codon_start=1
                     /product="bright green-yellow fluorescent protein derived 
                     from mClover2, with improved photostability (Bajar et al., 
                     2003)"
                     /label=mClover3
                     /note="mammalian codon-optimized"
                     /note="This feature has 3 segments:
                        1: 3243 .. 3245 / #05fd14
                        2: 3246 .. 3248 / #05fd14 / 1a
                        3: 3249 .. 3959 / #05fd14"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGVACFSRYPDHMKQHDFFKSAMPEGYVQERTISFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIK
                     ANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSHQSKLSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     misc_feature    3246..3959
                     /label=mCLOVER (no start/stop codon)
                     /note="color: #00ffff"
     primer_bind     3935..3984
                     /label=YTHDC1-YTH-IDR2_mCLOVER_60240_F
                     /note="color: black; sequence: 
                     acatggcatggacgagctGTACAAGtatgtgcttcaagatgcaagatttt; added: 
                     2019-07-01"
     primer_bind     complement(3935..3984)
                     /label=mCLOVER_YTHDC1-YTH-IDR2_60240_R
                     /note="color: black; sequence: 
                     aaaatcttgcatcttgaagcacataCTTGTACagctcgtccatgccatgt; added: 
                     2019-07-01"
     misc_feature    3960..5093
                     /label=YTHDC1-YTH-IDR2 (no start/stop codon)
                     /note="color: #ff9900"
     CDS             5094..5687
                     /codon_start=1
                     /gene="Mycobacterium xenopi gyrA"
                     /product="modified GyrA intein (Southworth et al., 1999)"
                     /label=Mxe GyrA intein
                     /note="N-terminal cleavage of this intein can be induced by
                     thiols."
                     /note="color: #cc99b2"
                     /translation="CITGDALVALPEGESVRIADIVPGARPNSDNAIDLKVLDRHGNPV
                     LADRLFHSGEHPVYTVRTVEGLRVTGTANHPLLCLVDVAGVPTLLWKLIDEIKPGDYAV
                     IQRSAFSVDCAGFARGKPEFAPTTYTVGVPGLVRFLEAHHRDPDAQAIADELTDGRFYY
                     AKVASVTDAGVQPVYSLRVDTADHAFITNGFVSHA"
     misc_feature    5094..5110
                     /label=Mxe GyrA Intein Repair
                     /note="color: #ffff99"
     misc_feature    5111..5116
                     /label=SpeI Enzyme Active Site
                     /note="color: #ff0000"
     CDS             5718..5873
                     /codon_start=1
                     /gene="Bacillus circulans chiA"
                     /product="chitin binding domain from chitinase A1 (Watanabe
                     et al., 1994)"
                     /label=CBD
                     /note="color: #cc99b2"
                     /translation="TTNPGVSAWQVNTAYTAGQLVTYNGKTYKCLQPHTSLAGWEPSNV
                     PALWQLQ"
     terminator      5952..5999
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
                     /note="color: #ffffff; direction: RIGHT"
     promoter        6059..6163
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: RIGHT"
     CDS             6164..7024
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 6164 .. 6232 / #ccffcc / signal sequence
                        2: 6233 .. 7024 / #ccffcc
                     Cleavage site after base 6232"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      complement(7066..7579)
                     /direction=LEFT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
     rep_origin      7690..8278
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
ORIGIN
        1 atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc
       61 cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac
      121 cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca
      181 gctgcggtaa agctcatcag cgtggtcgtg cagcgattca cagatgtctg cctgttcatc
      241 cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc
      301 catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct
      361 gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga
      421 tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg
      481 gcgggaccag agaaaaatca ctcagggtca atgccagccg aacgccagca agacgtagcc
      541 cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc
      601 gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag
      661 gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc
      721 cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt
      781 catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg
      841 agatcccggt gcctaatgag tgagctaact tacattaatt gcgttgcgct cactgcccgc
      901 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag
      961 aggcggtttg cgtattgggc gccagggtgg tttttctttt caccagtgag acgggcaaca
     1021 gctgattgcc cttcaccgcc tggccctgag agagttgcag caagcggtcc acgctggttt
     1081 gccccagcag gcgaaaatcc tgtttgatgg tggttaacgg cgggatataa catgagctgt
     1141 cttcggtatc gtcgtatccc actaccgaga tatccgcacc aacgcgcagc ccggactcgg
     1201 taatggcgcg cattgcgccc agcgccatct gatcgttggc aaccagcatc gcagtgggaa
     1261 cgatgccctc attcagcatt tgcatggttt gttgaaaacc ggacatggca ctccagtcgc
     1321 cttcccgttc cgctatcggc tgaatttgat tgcgagtgag atatttatgc cagccagcca
     1381 gacgcagacg cgccgagaca gaacttaatg ggcccgctaa cagcgcgatt tgctggtgac
     1441 ccaatgcgac cagatgctcc acgcccagtc gcgtaccgtc ttcatgggag aaaataatac
     1501 tgttgatggg tgtctggtca gagacatcaa gaaataacgc cggaacatta gtgcaggcag
     1561 cttccacagc aatggcatcc tggtcatcca gcggatagtt aatgatcagc ccactgacgc
     1621 gttgcgcgag aagattgtgc accgccgctt tacaggcttc gacgccgctt cgttctacca
     1681 tcgacaccac cacgctggca cccagttgat cggcgcgaga tttaatcgcc gcgacaattt
     1741 gcgacggcgc gtgcagggcc agactggagg tggcaacgcc aatcagcaac gactgtttgc
     1801 ccgccagttg ttgtgccacg cggttgggaa tgtaattcag ctccgccatc gccgcttcca
     1861 ctttttcccg cgttttcgca gaaacgtggc tggcctggtt caccacgcgg gaaacggtct
     1921 gataagagac accggcatac tctgcgacat cgtataacgt tactggtttc acattcacca
     1981 ccctgaattg actctcttcc gggcgctatc atgccatacc gcgaaaggtt ttgcgccatt
     2041 cgatggtgtc cgggatctcg acgctctccc ttatgcgact cctgcattag gaagcagccc
     2101 agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg ccggcatgcc
     2161 gccctttcgt cttcaagaat taattcccaa ttccccaggc atcaaataaa acgaaaggct
     2221 cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctcctgagt
     2281 aggacaaatc cgccgggagc ggatttgaac gttgcgaagc aacggcccgg agggtggcgg
     2341 gcaggacgcc cgccataaac tgccaggaat taattcccca ggcatcaaat aaaacgaaag
     2401 gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctcctg
     2461 agtaggacaa atccgccggg agcggatttg aacgttgcga agcaacggcc cggagggtgg
     2521 cgggcaggac gcccgccata aactgccagg aattaattcc ccaggcatca aataaaacga
     2581 aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc
     2641 ctgagtagga caaatccgcc gggagcggat ttgaacgttg cgaagcaacg gcccggaggg
     2701 tggcgggcag gacgcccgcc ataaactgcc aggaattaat tccccaggca tcaaataaaa
     2761 cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct
     2821 ctcctgagta ggacaaatcc gccgggagcg gatttgaacg ttgcgaagca acggcccgga
     2881 gggtggcggg caggacgccc gccataaact gccaggaatt aattccccag gcatcaaata
     2941 aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac
     3001 gctctcctga gtaggacaaa tccgccggga gcggatttga acgttgcgaa gcaacggccc
     3061 ggagggtggc gggcaggacg cccgccataa actgccagga attggggatc ggaattaatt
     3121 cccggtttaa accggggatc tcgatcccgc gaaattaata cgactcacta taggggaatt
     3181 gtgagcggat aacaattccc ctctagaaat aattttgtta actttaagaa ggagatatac
     3241 atatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg
     3301 acggcgacgt aaacggccac aagttcagcg tccgcggcga gggcgagggc gatgccacca
     3361 acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca
     3421 ccctcgtgac caccttcggc tacggcgtgg cctgcttcag ccgctacccc gaccacatga
     3481 agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct
     3541 ctttcaagga cgacggtacc tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc
     3601 tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc
     3661 acaagctgga gtacaacttc aacagccaca acgtctatat cacggccgac aagcagaaga
     3721 acggcatcaa ggctaacttc aagatccgcc acaacgttga ggacggcagc gtgcagctcg
     3781 ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc
     3841 actacctgag ccatcagtcc aagctgagca aagaccccaa cgagaagcgc gatcacatgg
     3901 tcctgctgga gttcgtgacc gccgccggga ttacacatgg catggacgag ctgtacaagt
     3961 atgtgcttca agatgcaaga tttttcctca taaagagtaa caaccatgag aatgtgtctc
     4021 ttgccaaagc gaagggtgta tggtccacgc tccctgtaaa tgagaagaaa ttaaatcttg
     4081 catttagatc tgcaaggagt gttatcttaa tattttctgt cagagagagt ggaaaatttc
     4141 aagggtttgc aagactttct tcagaatcac atcacggagg atctcctata cactgggtgc
     4201 ttccagcagg aatgagtgct aaaatgctgg gaggtgtctt taaaattgac tggatttgca
     4261 ggcgtgaatt acccttcact aagtcggctc atctcaccaa tccttggaat gaacataaac
     4321 cagtaaagat cggacgtgat ggacaggaaa ttgaacttga atgtggaacc cagctttgtc
     4381 ttctgtttcc ccccgatgaa agtattgact tgtatcaggt cattcataaa atgcgtcaca
     4441 agagaagaat gcattctcag ccccgatcac gaggacgtcc atcccgtcga gaaccagtcc
     4501 gggatgtggg aaggcgtcga ccagaagatt atgatattca taacagcaga aagaaaccaa
     4561 ggattgacta tccccctgag tttcaccaga gaccagggta tttaaaggat ccacgatacc
     4621 aggaagtgga cagacgattt tcaggagttc gccgagatgt gtttttaaat gggtcctaca
     4681 atgattatgt gagggaattt cataacatgg gaccaccacc accttggcaa ggaatgcccc
     4741 cttacccagg aatggaacaa cctccacacc atccttacta tcagcaccat gctccacctc
     4801 ctcaagctca tcccccttac tcaggacatc atccagtacc acatgaagca agatacagag
     4861 ataaacgagt acatgattat gatatgaggg tggatgattt ccttcgtcgc acacaagctg
     4921 ttgtcagtgg ccggagaagt agaccccgtg aaagagaccg ggaacgagag cgagaccgcc
     4981 ctagagataa cagacgagac agagagcgag atagaggacg tgatagagaa agagaaagag
     5041 agcgattatg tgatcgagac agagaccgag gggagagagg tcgatataga agatgcatca
     5101 cgggagatgc actagttgcc ctacccgagg gcgagtcggt acgcatcgcc gacatcgtgc
     5161 cgggtgcgcg gcccaacagt gacaacgcca tcgacctgaa agtccttgac cggcatggca
     5221 atcccgtgct cgccgaccgg ctgttccact ccggcgagca tccggtgtac acggtgcgta
     5281 cggtcgaagg tctgcgtgtg acgggcaccg cgaaccaccc gttgttgtgt ttggtcgacg
     5341 tcgccggggt gccgaccctg ctgtggaagc tgatcgacga aatcaagccg ggcgattacg
     5401 cggtgattca acgcagcgca ttcagcgtcg actgtgcagg ttttgcccgc gggaaacccg
     5461 aatttgcgcc cacaacctac acagtcggcg tccctggact ggtgcgtttc ttggaagcac
     5521 accaccgaga cccggacgcc caagctatcg ccgacgagct gaccgacggg cggttctact
     5581 acgcgaaagt cgccagtgtc accgacgccg gcgtgcagcc ggtgtatagc cttcgtgtcg
     5641 acacggcaga ccacgcgttt atcacgaacg ggttcgtcag ccacgctact ggcctcaccg
     5701 gtctgaactc aggcctcacg acaaatcctg gtgtatccgc ttggcaggtc aacacagctt
     5761 atactgcggg acaattggtc acatataacg gcaagacgta taaatgtttg cagccccaca
     5821 cctccttggc aggatgggaa ccatccaacg ttcctgcctt gtggcagctt caatgactgc
     5881 aggaagggga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg
     5941 ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc
     6001 tgaaaggagg aactatatcc ggataactac gtcaggtggc acttttcggg gaaatgtgcg
     6061 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
     6121 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
     6181 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
     6241 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
     6301 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gtttcccaat
     6361 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca
     6421 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
     6481 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
     6541 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
     6601 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
     6661 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac
     6721 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
     6781 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
     6841 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc
     6901 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
     6961 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
     7021 gtaactgtca gaccaagttt actcatatat actttagatt gatttacccc ggttgataat
     7081 cagaaaagcc ccaaaaacag gaagattgta taagcaaata tttaaattgt aaacgttaat
     7141 attttgttaa aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc
     7201 gaaatcggca aaatccctta taaatcaaaa gaatagcccg agatagggtt gagtgttgtt
     7261 ccagtttgga acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa
     7321 accgtctatc agggcgatgg cccactacgt gaaccatcac ccaaatcaag ttttttgggg
     7381 tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga gcccccgatt tagagcttga
     7441 cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct
     7501 agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat
     7561 gcgccgctac agggcgcgta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     7621 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     7681 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     7741 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     7801 aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg
     7861 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     7921 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     7981 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     8041 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     8101 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     8161 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     8221 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     8281 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
     8341 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
     8401 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagctatggt
     8461 gcactctcag tacaatctgc tctgatgccg catagttaag ccagt
//
