LOCUS       pBS_minimal_PB          3071 bp    DNA        circular     17-JAN-2024
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_feature    262..278
                     /note="M13R"
                     /locus_tag="M13rev"
     misc_feature    299..317
                     /note="T3"
                     /locus_tag="T3"
     misc_feature    complement(335..401)
                     /note="3' piggyBac sequence"
                     /locus_tag="3' piggyBac ITR"
     misc_feature    335..338
                     /note="TTAA"
                     /locus_tag="TTAA"
     misc_feature    339..351
                     /note="13 bp terminal inverted repeat"
                     /locus_tag="13-bp TR(1)"
     misc_feature    352..382
                     /note="31 bp spacer"
                     /locus_tag="31 bp spacer"
     misc_feature    383..401
                     /note="19 bp internal inverted repeat"
                     /locus_tag="19-bp IR"
     misc_feature    complement(486..524)
                     /note="5' piggyBac sequence"
                     /locus_tag="5' piggyBac ITR"
     misc_feature    486..504
                     /note="19 bp internal inverted repeat"
                     /locus_tag="19-bp TR"
     misc_feature    505..507
                     /note="3 bp spacer"
                     /locus_tag="3 bp spacer"
     misc_feature    508..520
                     /note="13 bp terminal inverted repeat"
                     /locus_tag="13-bp TR"
     misc_feature    521..524
                     /note="TTAA"
                     /locus_tag="TTAA(1)"
     misc_feature    558..576
                     /note="T7"
                     /locus_tag="T7"
     misc_feature    583..599
                     /note="M13F"
                     /locus_tag="M13for"
     CDS             complement(873..1179)
                     /locus_tag="f1 origin"
     CDS             1328..2185
                     /locus_tag="Amp+"
     misc_feature    complement(2336..3003)
                     /locus_tag="pUC origin"
ORIGIN
        1 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
       61 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg
      121 acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca
      181 ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg
      241 tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgcc aagcgcgcaa
      301 ttaaccctca ctaaagggaa cctcccccaa ttccttaacc ctagaaagat aatcatattg
      361 tgacgtacgt taaagataat catgcgtaaa attgacgcat gaggtctgaa gaggagttta
      421 cgtccagcca aaccggtcat ggatccatga agcttgctcg actagagctt gcggaaccct
      481 tcgaacatgc gtcaatttta cgcagactat ctttctaggg ttaaatcagt caggtacata
      541 atggatcttt gggtcgccct atagtgagtc gtattacaat tcactggccg tcgttttaca
      601 acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc
      661 tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg
      721 cagcctgaat ggcgaatggc aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa
      781 tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa
      841 atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact
      901 attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc
      961 actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa
     1021 tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc
     1081 gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt
     1141 cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtcagg
     1201 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
     1261 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
     1321 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg
     1381 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt
     1441 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt
     1501 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt
     1561 attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa
     1621 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag
     1681 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac
     1741 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac
     1801 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac
     1861 cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac
     1921 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact
     1981 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg
     2041 tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt
     2101 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat
     2161 aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta
     2221 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
     2281 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
     2341 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     2401 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     2461 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
     2521 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     2581 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     2641 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     2701 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
     2761 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     2821 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     2881 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     2941 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
     3001 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga
     3061 gtgagctgat a    
//