LOCUS       pRS42K_MCP-VP64_C-MS2   8026 bp ds-DNA     circular     22-SEP-2021
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    complement(244..1590)
                     /label="2レm"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     CDS             1722..2582
                     /label="AmpR"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     rep_origin      complement(3285..3357)
                     /label="ori"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    3764..3783
                     /label="T3"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    3801..4505
                     /label="ADH1 promoter"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    4524..5090
                     /label="MCP/VP64"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     CDS             4524..5090
                     /label="Translation 4524-5090"
     misc_feature    4539..4559
                     /label="NLS"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    4932..5081
                     /label="VP64"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    5091..5491
                     /label="ENO2 term"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    5498..5916
                     /label="scRNA-tet/MS2"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    5502..5770
                     /label="SNR52 promoter"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    5772..5791
                     /label="TS syn4"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    5793..5867
                     /label="structural crRNA"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    5868..5892
                     /label="MS2"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    5893..5912
                     /label="SUP4 term"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    complement(5930..5949)
                     /label="T7"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     terminator      complement(6629..6868)
                     /label="TEF1t"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     CDS             complement(6869..7678)
                     /label="kanMX"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     promoter        complement(7679..8026)
                     /label="TEF1p"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
ORIGIN
        1 cactctcagt acaatctgct ctgatgccgc atagttaagc cagccccgac acccgccaac
       61 acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt
      121 gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag
      181 acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc
      241 ttagtatgat ccaatatcaa aggaaatgat agcattgaag gatgagacta atccaattga
      301 ggagtggcag catatagaac agctaaaggg tagtgctgaa ggaagcatac gataccccgc
      361 atggaatggg ataatatcac aggaggtact agactacctt tcatcctaca taaatagacg
      421 catataagta cgcatttaag cataaacacg cactatgccg ttcttctcat gtatatatat
      481 atacaggcaa cacgcagata taggtgcgac gtgaacagtg agctgtatgt gcgcagctcg
      541 cgttgcattt tcggaagcgc tcgttttcgg aaacgctttg aagttcctat tccgaagttc
      601 ctattctcta gaaagtatag gaacttcaga gcgcttttga aaaccaaaag cgctctgaag
      661 acgcactttc aaaaaaccaa aaacgcaccg gactgtaacg agctactaaa atattgcgaa
      721 taccgcttcc acaaacattg ctcaaaagta tctctttgct atatatctct gtgctatatc
      781 cctatataac ctacccatcc acctttcgct ccttgaactt gcatctaaac tcgacctcta
      841 cattttttat gtttatctct agtattactc tttagacaaa aaaattgtag taagaactat
      901 tcatagagtg aatcgaaaac aatacgaaaa tgtaaacatt tcctatacgt agtatataga
      961 gacaaaatag aagaaaccgt tcataatttt ctgaccaatg aagaatcatc aacgctatca
     1021 ctttctgttc acaaagtatg cgcaatccac atcggtatag aatataatcg gggatgcctt
     1081 tatcttgaaa aaatgcaccc gcagcttcgc tagtaatcag taaacgcggg aagtggagtc
     1141 aggctttttt tatggaagag aaaatagaca ccaaagtagc cttcttctaa ccttaacgga
     1201 cctacagtgc aaaaagttat caagagactg cattatagag cgcacaaagg agaaaaaaag
     1261 taatctaaga tgctttgtta gaaaaatagc gctctcggga tgcatttttg tagaacaaaa
     1321 aagaagtata gattctttgt tggtaaaata gcgctctcgc gttgcatttc tgttctgtaa
     1381 aaatgcagct cagattcttt gtttgaaaaa ttagcgctct cgcgttgcat ttttgtttta
     1441 caaaaatgaa gcacagattc ttcgttggta aaatagcgct ttcgcgttgc atttctgttc
     1501 tgtaaaaatg cagctcagat tctttgtttg aaaaattagc gctctcgcgt tgcatttttg
     1561 ttctacaaaa tgaagcacag atgcttcgtt caggtggcac ttttcgggga aatgtgcgcg
     1621 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
     1681 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
     1741 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
     1801 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
     1861 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
     1921 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag
     1981 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
     2041 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
     2101 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
     2161 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
     2221 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa
     2281 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
     2341 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
     2401 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
     2461 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
     2521 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
     2581 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
     2641 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
     2701 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     2761 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     2821 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     2881 cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact
     2941 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     3001 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     3061 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     3121 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
     3181 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     3241 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     3301 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
     3361 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
     3421 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
     3481 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac
     3541 cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact
     3601 ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta cctcactcat taggcacccc
     3661 aggctttaca ctttatgctt ccggctccta tgttgtgtgg aattgtgagc ggataacaat
     3721 ttcacacagg aaacagctat gaccatgatt acgccaagcg cgcaattaac cctcactaaa
     3781 gggaacaaaa gctggagctc atccttttgt tgtttccggg tgtacaatat ggacttcctc
     3841 ttttctggca accaaaccca tacatcggga ttcctataat accttcgttg gtctccctaa
     3901 catgtaggtg gcggagggga gatatacaat agaacagata ccagacaaga cataatgggc
     3961 taaacaagac tacaccaatt acactgcctc attgatggtg gtacataacg aactaatact
     4021 gtagccctag acttgatagc catcatcata tcgaagtttc actacccttt ttccatttgc
     4081 catctattga agtaataata ggcgcatgca acttcttttc tttttttttc ttttctctct
     4141 cccccgttgt tgtctcacca tatccgcaat gacaaaaaaa tgatggaaga cactaaagga
     4201 aaaaattaac gacaaagaca gcaccaacag atgtcgttgt tccagagctg atgaggggta
     4261 tctcgaagca cacgaaactt tttccttcct tcattcacgc acactactct ctaatgagca
     4321 acggtatacg gccttccttc cagttacttg aatttgaaat aaaaaaaagt ttgctgtctt
     4381 gctatcaagt ataaatagac ctgcaattat taatcttttg tttcctcgtc attgttctcg
     4441 ttccctttct tccttgtttc tttttctgca caatatttca agctatacca agcatacaat
     4501 caacttctag aactagtaaa acaatgtctc tTgagatgcc caaaaagaaa agaaaagtgg
     4561 gtagtatggc ttctaacttt actcagttcg ttctcgtcga caatggcgga actggcgacg
     4621 tgactgtcgc cccaagcaac ttcgctaacg ggatcgctga atggatcagc tctaactcgc
     4681 gttcacaggc ttacaaagta acctgtagcg ttcgtcagag ctctgcgcag aatcgcaaat
     4741 acaccatcaa agtcgaggtg cctaaaggcg cctggcgttc gtacttaaat atggaactaa
     4801 ccattccaat tttcgccacg aattccgact gcgagcttat tgttaaggca atgcaaggtc
     4861 tcctaaaaga tggaaacccg attccctcag caatcgcagc aaactccggc atctacggct
     4921 ccggacgagc cgatgctctc gacgattttg acctcgatat gctgggatcc gacgctctcg
     4981 atgacttcga ccttgacatg ctggggagtg acgctctgga cgacttcgat ctcgacatgc
     5041 tgggctccga cgccctggat gacttcgatc tggacatgct catcaactga agtgctttta
     5101 actaagaatt attagtcttt tctgcttatt ttttcatcat agtttagaac actttatatt
     5161 aacgaatagt ttatgaatct atttaggttt aaaaattgat acagttttat aagttacttt
     5221 ttcaaagact cgtgctgtct attgcataat gcactggaag gggaaaaaaa aggtgcacac
     5281 gcgtggcttt ttcttgaatt tgcagtttga aaaataacta catggatgat aagaaaacat
     5341 ggagtacagt cactttgaga accttcaatc agctggtaac gtcttcgtta attggatact
     5401 caaaaaagat ggatagcatg aatcacaaga tggaaggaaa tgcgggccac gaccacagtg
     5461 atatgcatat gggagatgga gatgatacct gcccgggccg gtctttgaaa agataatgta
     5521 tgattatgct ttcactcata tttatacaga aacttgatgt tttctttcga gtatatacaa
     5581 ggtgattaca tgtacgtttg aagtacaact ctagattttg tagtgccctc ttgggctagc
     5641 ggtaaaggtg cgcatttttt cacaccctac aatgttctgt tcaaaagatt ttggtcaaac
     5701 gctgtagaag tgaaagttgg tgcgcatgtt tcggcgttcg aaacttctcc gcagtgaaag
     5761 ataaatgatc gTTCAACGCG TTCACGTGTG Ggttttagag ctagaaatag caagttaaaa
     5821 taaggctagt ccgttatcaa cttgaaaaag tggcaccgag tcggtgcGCG CACATGAGGA
     5881 TCACCCATGT GCTTTTTTTG TTTTTTATGT CTccggggta cccaattcgc cctatagtga
     5941 gtcgtattac gcgcgctcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg
     6001 cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga
     6061 agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatggcgcga
     6121 cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc
     6181 tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac
     6241 gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag
     6301 tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc
     6361 atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg
     6421 actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata
     6481 agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa
     6541 cgcgaatttt aacaaaatat taacgtttac aatttcctga tgcggtattt tctccttacg
     6601 catctgtgcg gtatttcaca ccgcgttttc gacactggat ggcggcgtta gtatcgaatc
     6661 gacagcagta tagcgaccag cattcacata cgattgacgc atgatattac tttctgcgca
     6721 cttaacttcg catctgggca gatgatgtcg aggcgaaaaa aaatataaat cacgctaaca
     6781 tttgattaaa atagaacaac tacaatataa aaaaactata caaatgacaa gttcttgaaa
     6841 acaagaatct ttttattgtc agtactgatt agaaaaactc atcgagcatc aaatgaaact
     6901 gcaatttatt catatcagga ttatcaatac catatttttg aaaaagccgt ttctgtaatg
     6961 aaggagaaaa ctcaccgagg cagttccata ggatggcaag atcctggtat cggtctgcga
     7021 ttccgactcg tccaacatca atacaaccta ttaatttccc ctcgtcaaaa ataaggttat
     7081 caagtgagaa atcaccatga gtgacgactg aatccggtga gaatggcaaa agcttatgca
     7141 tttctttcca gacttgttca acaggccagc cattacgctc gtcatcaaaa tcactcgcat
     7201 caaccaaacc gttattcatt cgtgattgcg cctgagcgag acgaaatacg cgatcgctgt
     7261 taaaaggaca attacaaaca ggaatcgaat gcaaccggcg caggaacact gccagcgcat
     7321 caacaatatt ttcacctgaa tcaggatatt cttctaatac ctggaatgct gttttgccgg
     7381 ggatcgcagt ggtgagtaac catgcatcat caggagtacg gataaaatgc ttgatggtcg
     7441 gaagaggcat aaattccgtc agccagttta gtctgaccat ctcatctgta acatcattgg
     7501 caacgctacc tttgccatgt ttcagaaaca actctggcgc atcgggcttc ccatacaatc
     7561 gatagattgt cgcacctgat tgcccgacat tatcgcgagc ccatttatac ccatataaat
     7621 cagcatccat gttggaattt aatcgcggcc tcgaaacgtg agtcttttcc ttacccatgg
     7681 ttgtttatgt tcggatgtga tgtgagaact gtatcctagc aagattttaa aaggaagtat
     7741 atgaaagaag aacctcagtg gcaaatccta accttttata tttctctaca ggggcgcggc
     7801 gtggggacaa ttcaacgcgt ctgtgagggg agcgtttccc tgctcgcagg tctgcagcga
     7861 ggagccgtaa tttttgcttc gcgccgtgcg gccatcaaaa tgtatggatg caaatgatta
     7921 tacatgggga tgtatgggct aaatgtacgg gcgacagtca catcatgccc ctgagctgcg
     7981 cacgtcaaga ctgtcaagga gggtattctg ggcctccatg tcgctg
//