LOCUS       pRS426-PGAL7-D_RFP_TCYC 6799 bp ds-DNA     circular     22-SEP-2021
DEFINITION  .
FEATURES             Location/Qualifiers
     rep_origin      complement(66..1411)
                     /label="2_MICRON"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     CDS             1544..2401
                     /label="AP(R)"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     rep_origin      complement(2404..3344)
                     /label="PMB1"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     promoter        3438..3559
                     /label="P(LAC)"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     promoter        3585..3604
                     /label="T3_P"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    3622..3641
                     /label="syn5 TS .63 0"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    3622..3809
                     /label="GAL7syn5"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    3822..4532
                     /label="yeCherry"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     terminator      complement(4546..4785)
                     /label="CYC1"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     promoter        complement(4804..4823)
                     /label="T7_P"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     rep_origin      4993..5449
                     /label="F1_ORI"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     CDS             complement(5580..6383)
                     /label="URA3"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttagtatga tccaatatca aaggaaatga tagcattgaa ggatgagact aatccaattg
      121 aggagtggca gcatatagaa cagctaaagg gtagtgctga aggaagcata cgataccccg
      181 catggaatgg gataatatca caggaggtac tagactacct ttcatcctac ataaatagac
      241 gcatataagt acgcatttaa gcataaacac gcactatgcc gttcttctca tgtatatata
      301 tatacaggca acacgcagat ataggtgcga cgtgaacagt gagctgtatg tgcgcagctc
      361 gcgttgcatt ttcggaagcg ctcgttttcg gaaacgcttt gaagttccta ttccgaagtt
      421 cctattctct agaaagtata ggaacttcag agcgcttttg aaaaccaaaa gcgctctgaa
      481 gacgcacttt caaaaaacca aaaacgcacc ggactgtaac gagctactaa aatattgcga
      541 ataccgcttc cacaaacatt gctcaaaagt atctctttgc tatatatctc tgtgctatat
      601 ccctatataa cctacccatc cacctttcgc tccttgaact tgcatctaaa ctcgacctct
      661 acatttttta tgtttatctc tagtattact ctttagacaa aaaaattgta gtaagaacta
      721 ttcatagagt gaatcgaaaa caatacgaaa atgtaaacat ttcctatacg tagtatatag
      781 agacaaaata gaagaaaccg ttcataattt tctgaccaat gaagaatcat caacgctatc
      841 actttctgtt cacaaagtat gcgcaatcca catcggtata gaatataatc ggggatgcct
      901 ttatcttgaa aaaatgcacc cgcagcttcg ctagtaatca gtaaacgcgg gaagtggagt
      961 caggcttttt ttatggaaga gaaaatagac accaaagtag ccttcttcta accttaacgg
     1021 acctacagtg caaaaagtta tcaagagact gcattataga gcgcacaaag gagaaaaaaa
     1081 gtaatctaag atgctttgtt agaaaaatag cgctctcggg atgcattttt gtagaacaaa
     1141 aaagaagtat agattctttg ttggtaaaat agcgctctcg cgttgcattt ctgttctgta
     1201 aaaatgcagc tcagattctt tgtttgaaaa attagcgctc tcgcgttgca tttttgtttt
     1261 acaaaaatga agcacagatt cttcgttggt aaaatagcgc tttcgcgttg catttctgtt
     1321 ctgtaaaaat gcagctcaga ttctttgttt gaaaaattag cgctctcgcg ttgcattttt
     1381 gttctacaaa atgaagcaca gatgcttcgt tcaggtggca cttttcgggg aaatgtgcgc
     1441 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
     1501 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
     1561 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
     1621 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
     1681 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
     1741 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa
     1801 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
     1861 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
     1921 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
     1981 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
     2041 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca
     2101 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
     2161 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
     2221 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
     2281 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
     2341 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
     2401 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
     2461 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
     2521 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     2581 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     2641 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     2701 gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac
     2761 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     2821 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     2881 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     2941 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     3001 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     3061 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     3121 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     3181 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     3241 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
     3301 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa
     3361 ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac
     3421 tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt acctcactca ttaggcaccc
     3481 caggctttac actttatgct tccggctcct atgttgtgtg gaattgtgag cggataacaa
     3541 tttcacacag gaaacagcta tgaccatgat tacgccaagc gcgcaattaa ccctcactaa
     3601 agggaacaaa agctggagct ctctctatca ctgataggga gCGGACTGGC TATACAGTGT
     3661 TCACAAAATA GCCAAGCTGA AAATAATGTG TAGCTATGTT CAGTTAGTTT GGCTAGCAAA
     3721 GATATAAAAG CAGGTCGGAA ATATTTATGG GCATTATTAT GCAGAGCATC AACATGATAA
     3781 AAAAAAACAG TTGAATATTC CCTCAAAAAa ctagtaaaac aatggtaagt aagggtgaag
     3841 aagacaatat ggcgatcatt aaggaattca tgcgtttcaa agtacacatg gagggaagcg
     3901 tgaacggaca tgaatttgaa atcgaagggg aaggcgaagg tagaccatac gaaggaaccc
     3961 agaccgcaaa gcttaaagtt accaaaggcg ggccactacc atttgcatgg gatatcttga
     4021 gccctcagtt tatgtatggc agtaaggcct acgttaaaca cccagctgat attcccgact
     4081 atttgaaatt gtcttttcca gaaggattca aatgggaaag agtaatgaat ttcgaggacg
     4141 gcggagttgt tactgttact caagattcaa gtttgcaaga cggtgaattt atttacaagg
     4201 tcaaattaag agggactaat ttccctagtg atggtcccgt catgcaaaag aagactatgg
     4261 gttgggaagc ctcatctgaa cgtatgtatc cagaagatgg cgcgcttaag ggggaaatta
     4321 aacaaagatt gaagttaaaa gacggtggtc actacgacgc ggaagttaag accacttata
     4381 aagctaaaaa gcccgttcag ttacctggtg catataacgt aaacattaaa ttggatatca
     4441 cttcacataa tgaagattac actattgtgg aacaatatga aagagctgaa ggtaggcact
     4501 caacgggtgg aatggacgaa ttgtacaaat aactcgagtc atgtaattag ttatgtcacg
     4561 cttacattca cgccctcccc ccacatccgc tctaaccgaa aaggaaggag ttagacaacc
     4621 tgaagtctag gtccctattt atttttttat agttatgtta gtattaagaa cgttatttat
     4681 atttcaaatt tttctttttt ttctgtacag acgcgtgtac gcatgtaaca ttatactgaa
     4741 aaccttgctt gagaaggttt tgggacgctc gaaggcttta atttgcggcc ggtacccaat
     4801 tcgccctata gtgagtcgta ttacgcgcgc tcactggccg tcgttttaca acgtcgtgac
     4861 tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc
     4921 tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat
     4981 ggcgaatggc gcgacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
     5041 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct
     5101 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
     5161 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt
     5221 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
     5281 ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat
     5341 tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt
     5401 taacaaaaat ttaacgcgaa ttttaacaaa atattaacgt ttacaatttc ctgatgcggt
     5461 attttctcct tacgcatctg tgcggtattt cacaccgcat agggtaataa ctgatataat
     5521 taaattgaag ctctaatttg tgagtttagt atacatgcat ttacttataa tacagttttt
     5581 tagttttgct ggccgcatct tctcaaatat gcttcccagc ctgcttttct gtaacgttca
     5641 ccctctacct tagcatccct tccctttgca aatagtcctc ttccaacaat aataatgtca
     5701 gatcctgtag agaccacatc atccacggtt ctatactgtt gacccaatgc gtctcccttg
     5761 tcatctaaac ccacaccggg tgtcataatc aaccaatcgt aaccttcatc tcttccaccc
     5821 atgtctcttt gagcaataaa gccgataaca aaatctttgt cgctcttcgc aatgtcaaca
     5881 gtacccttag tatattctcc agtagatagg gagcccttgc atgacaattc tgctaacatc
     5941 aaaaggcctc taggttcctt tgttacttct tctgccgcct gcttcaaacc gctaacaata
     6001 cctgggccca ccacaccgtg tgcattcgta atgtctgccc attctgctat tctgtataca
     6061 cccgcagagt actgcaattt gactgtatta ccaatgtcag caaattttct gtcttcgaag
     6121 agtaaaaaat tgtacttggc ggataatgcc tttagcggct taactgtgcc ctccatggaa
     6181 aaatcagtca agatatccac atgtgttttt agtaaacaaa ttttgggacc taatgcttca
     6241 actaactcca gtaattcctt ggtggtacga acatccaatg aagcacacaa gtttgtttgc
     6301 ttttcgtgca tgatattaaa tagcttggca gcaacaggac taggatgagt agcagcacgt
     6361 tccttatatg tagctttcga catgatttat cttcgtttcc tgcaggtttt tgttctgtgc
     6421 agttgggtta agaatactgg gcaatttcat gtttcttcaa cactacatat gcgtatatat
     6481 accaatctaa gtctgtgctc cttccttcgt tcttccttct gttcggagat taccgaatca
     6541 aaaaaatttc aaagaaaccg aaatcaaaaa aaagaataaa aaaaaaatga tgaattgaat
     6601 tgaaaagctg tggtatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc
     6661 cagccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca
     6721 tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg
     6781 tcatcaccga aacgcgcga
//