LOCUS       TK1147                  9253 bp ds-DNA     circular     23-SEP-2021
DEFINITION  .
FEATURES             Location/Qualifiers
     CDS             330..989
                     /label="AmpR"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     rep_origin      1087..1769
                     /label="ColE1 origin"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_binding    2109..2131
                     /label="LacO"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     primer_bind     2137..2157
                     /label="M13-rev"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     primer_bind     2175..2194
                     /label="T3"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     LTR             2448..2628
                     /label="HIV LTR 1"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    3290..3523
                     /label="RRE"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    4016..4031
                     /label="cPPT"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_recomb     4181..4185
                     /label="AttL1"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     promoter        4191..4726
                     /label="mCMV promoter"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    4735..5169
                     /label="TOMM20"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     CDS             4735..5169
                     /label="Translation 4735-5169"
     misc_feature    5170..5202
                     /label="Nextera primer binding"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     CDS             5170..5202
                     /label="Translation 5170-5202"
     misc_feature    5203..5211
                     /label="This part is variable"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    5203..5211
                     /label="SQV variables"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     CDS             5203..5220
                     /label="Translation 5203-5220"
     misc_feature    5212..5220
                     /label="AscI"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    5213..5235
                     /label="digestion"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_feature    5213..5235
                     /label="digestion"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    5221..5226
                     /label="BamHI"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     CDS             5227..5235
                     /label="Translation 5227-5235"
     misc_feature    complement(5227..5241)
                     /label="rev"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    5227..5247
                     /label="extra"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     CDS             5236..5241
                     /label="Translation 5236-5241"
     CDS             5242..5628
                     /label="Translation 5242-5628"
     CDS             5248..5625
                     /label="H2B"
                     /ApEinfo_revcolor=#008040
                     /ApEinfo_fwdcolor=#008040
     misc_feature    5639..5660
                     /label="digestion"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    5645..5652
                     /label="extra cut sit"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_feature    5665..6345
                     /label="Clover"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_recomb     complement(6372..6374)
                     /label="AttL2"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     3'UTR           6429..7004
                     /label="WPRE"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     promoter        7067..7409
                     /label="SV40 early promoter"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     promoter        7449..7514
                     /label="EM7 promoter"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     CDS             7515..7913
                     /label="Blast cds"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    7977..7992
                     /label="cPPT"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     LTR             8047..8227
                     /label="HIV LTR 2"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     primer_bind     complement(8635..8652)
                     /label="M13-fwd"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     CDS             8723..8791
                     /label="LacZ alpha"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     rep_origin      8796..9251
                     /label="M13 origin"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     rep_origin      complement(8801..9241)
                     /label="F1 ori"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
ORIGIN
        1 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
       61 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
      121 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
      181 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
      241 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
      301 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
      361 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
      421 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
      481 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
      541 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
      601 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
      661 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
      721 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
      781 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
      841 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
      901 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
      961 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     1021 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     1081 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     1141 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     1201 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     1261 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt
     1321 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     1381 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     1441 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     1501 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     1561 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     1621 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     1681 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     1741 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     1801 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     1861 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     1921 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
     1981 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
     2041 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
     2101 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt
     2161 acgccaagcg cgcaattaac cctcactaaa gggaacaaaa gctggagctg caagcttaat
     2221 gtagtcttat gcaatactct tgtagtcttg caacatggta acgatgagtt agcaacatgc
     2281 cttacaagga gagaaaaagc accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg
     2341 tgccttatta ggaaggcaac agacgggtct gacatggatt ggacgaacca ctgaattgcc
     2401 gcattgcaga gatattgtat ttaagtgcct agctcgatac aataaacggg tctctctggt
     2461 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
     2521 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
     2581 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
     2641 cagggacctg aaagcgaaag ggaaaccaga gctctctcga cgcaggactc ggcttgctga
     2701 agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag
     2761 cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag
     2821 atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca
     2881 tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac
     2941 atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga
     3001 agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga
     3061 gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac
     3121 caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt
     3181 ggagaagtga attatataaa tataaagtag taaaaattga accattagga gtagcaccca
     3241 ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt
     3301 tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcctcaatg acgctgacgg
     3361 tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta
     3421 ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa
     3481 gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct
     3541 ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc
     3601 tggaacagat tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac
     3661 aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga
     3721 attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct
     3781 gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt
     3841 tgctgtactt tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac
     3901 ccacctccca accccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga
     3961 gagagacaga gacagatcca ttcgattagt gaacggatct cgacggttaa cttttaaaag
     4021 aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa tagcaacaga
     4081 catacaaact aaagaattac aaaaacaaat tacaaaaatt caaaatttta tTCCAGTGTG
     4141 GTGGAATTCT GCAGATATCA ACAAGTTTGT ACAAAAAAGC AGGCTtcgag tggcacgtat
     4201 actgagtcat tagggacttt ccaatgggtt ttgcccagta cataaggtca ataggggtga
     4261 atcaacagga aagtcccatt ggagccaagt acactgagtc aatagggact ttccattggg
     4321 ttttgcccag tacaaaaggt caataggggg tgagtcaatg ggtttttccc attattggca
     4381 cgtacataag gtcaataggg gtgagtcatt gggtttttcc agccaattta attaaaacgc
     4441 catgtacttt cccaccattg acgtcaatgg gctattgaaa ctaatgcaac gtgaccttta
     4501 aacggtactt tcccatagct gattaatggg aaagtaccgt tctcgagcca atacacgtca
     4561 atgggaagtg aaagggcagc caaaacgtaa caccgccccg gttttcccct ggaaattcca
     4621 tattggcacg cattctattg gctgagctgc gttctacgtg ggtataagag gcgcgaccag
     4681 cgtcggAacc gtcgcagtct tcggtctgac caccgtagaa cgcagaGTga TccaATGGTG
     4741 GGTCGGAACA GCGCCATCGC CGCCGGTGTA TGCGGGGCCC TTTTCATTGG GTACTGCATC
     4801 TACTTCGACC GCAAAAGACG AAGTGACCCC AACTTCAAGA ACAGGCTTCG AGAACGAAGA
     4861 AAGAAACAGA AGCTTGCCAA GGAGAGAGCT GGGCTTTCCA AGTTACCTGA CCTTAAAGAT
     4921 GCTGAAGCTG TTCAGAAGTT CTTCCTTGAA GAAATACAGC TTGGTGAAGA GTTACTAGCT
     4981 CAAGGTGAAT ATGAGAAGGG CGTAGACCAT CTGACAAATG CAATTGCTGT GTGTGGACAG
     5041 CCACAGCAGT TACTGCAGGT CTTACAGCAA ACTCTTCCAC CACCAGTGTT CCAGATGCTT
     5101 CTGACTAAGC TCCCAACAAT TAGTCAGAGA ATTGTAAGTG CTCAGAGCTT GGCTGAAGAT
     5161 GATGTGGAAT CGTCGGCAGC GTCAGATGTG TATAAGAGAC AGtcacagag tgggcgcgcc
     5221 GGATCCagcg atcgcctcga gACCGGTatg ccagagccag cgaagtctgc tcccgccccg
     5281 aaaaagggct ccaagaaggc ggtgactaag gcgcagaaga aaggcggcaa gaagcgcaag
     5341 cgcagccgca aggagagcta ttccatctat gtgtacaagg ttctgaagca ggtccaccct
     5401 gacaccggca tttcgtccaa ggccatgggc atcatgaatt cgtttgtgaa cgacattttc
     5461 gagcgcatcg caggtgaggc ttcccgcctg gcgcattaca acaagcgctc gaccatcacc
     5521 tccagggaga tccagacggc cgtgcgcctg ctgctgcctg gggagttggc caagcacgcc
     5581 gtgtccgagg gtactaaggc catcaccaag tacaccagcg ctaagTAGgc cgcacatagg
     5641 taccGCCCGG GCcctgcagg aATGgtgagc aagggcgagg agctgttcac cggggtggtg
     5701 cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt CCGCggcgag
     5761 ggcgagggcg atgccaccAA Cggcaagctg accctgaagt tcatctgcac caccggcaag
     5821 ctgcccgtgc cctggcccac cctcgtgacc accttcggct acggcgtggc ctgcttcagc
     5881 cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac
     5941 gtccaggagc gcaccatctc tttcaaggac gacggAacct acaagacccg cgccgaggtg
     6001 aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag
     6061 gacggcaaca tcctggggca caagctggag tacaacttca acagccacaa cgtctatatc
     6121 acggccgaca agcagaagaa cggcatcaag gctaacttca agatccgcca caacGTTgag
     6181 gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc
     6241 gtgctgctgc ccgacaacca ctacctgagc CATcagtccg ccctgagcaa agaccccaac
     6301 gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccTAAGC GGCCGCACTC
     6361 GAGATAtcta gACCCAGCTT TCTTGTACAA AGTGGTTGAT ATCCAGCACA GTGGCGGCCG
     6421 CTCGAcaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat
     6481 gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct
     6541 tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag
     6601 gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc
     6661 cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc
     6721 ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct
     6781 cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg
     6841 ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg
     6901 gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg
     6961 cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcctggA
     7021 ATTCTGCAGA TATCCGGTTA GTAATGAGTT TGGAATTAAT TCTGTGGAAT GTGTGTCAGT
     7081 TAGGGTGTGG AAAGTCCCCA GGCTCCCCAG GCAGGCAGAA GTATGCAAAG CATGCATCTC
     7141 AATTAGTCAG CAACCAGGTG TGGAAAGTCC CCAGGCTCCC CAGCAGGCAG AAGTATGCAA
     7201 AGCATGCATC TCAATTAGTC AGCAACCATA GTCCCGCCCC TAACTCCGCC CATCCCGCCC
     7261 CTAACTCCGC CCAGTTCCGC CCATTCTCCG CCCCATGGCT GACTAATTTT TTTTATTTAT
     7321 GCAGAGGCCG AGGCCGCCTC TGCCTCTGAG CTATTCCAGA AGTAGTGAGG AGGCTTTTTT
     7381 GGAGGCCTAG GCTTTTGCAA AAAGCTCCCG GGAGCTTGTA TATCCATTTT CGGATCTGAT
     7441 CAGCACGTGT TGACAATTAA TCATCGGCAT AGTATATCGG CATAGTATAA TACGACAAGG
     7501 TGAGGAACTA AACCATGGCC AAGCCTTTGT CTCAAGAAGA ATCCACCCTC ATTGAAAGAG
     7561 CAACGGCTAC AATCAACAGC ATCCCCATCT CTGAAGACTA CAGCGTCGCC AGCGCAGCTC
     7621 TCTCTAGCGA CGGCCGCATC TTCACTGGTG TCAATGTATA TCATTTTACT GGGGGACCTT
     7681 GTGCAGAACT CGTGGTGCTG GGCACTGCTG CTGCTGCGGC AGCTGGCAAC CTGACTTGTA
     7741 TCGTCGCGAT CGGAAATGAG AACAGGGGCA TCTTGAGCCC CTGCGGACGG TGCCGACAGG
     7801 TGCTTCTCGA TCTGCATCCT GGGATCAAAG CCATAGTGAA GGACAGTGAT GGACAGCCGA
     7861 CGGCAGTTGG GATTCGTGAA TTGCTGCCCT CTGGTTATGT GTGGGAGGGC TAAGCACAAT
     7921 TCGAGCTCtt taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa
     7981 gaaaaggggg gactggaagg gctagctcac tcccaacgaa gacaagatct gctttttgct
     8041 tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctgcctaggg
     8101 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt
     8161 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc
     8221 tctagcagta gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga
     8281 atatcagaga gtgagaggaa cttgtttatt gcagcttata atggttacaa ataaagcaat
     8341 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
     8401 aaactcatca atgtatctta tcatgtctgg ctctagctat cccgccccta actccgccca
     8461 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     8521 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     8581 tttgcgtcga gacgtaccca attcgcccta tagtgagtcg tattacgcgc gctcactggc
     8641 cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc
     8701 agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc
     8761 ccaacagttg cgcagcctga atggcgaatg gcgcgacgcg ccctgtagcg gcgcattaag
     8821 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc
     8881 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc
     8941 tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa
     9001 aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg
     9061 ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac
     9121 actcaaccct atctcggtct attcttttga tttataaggg attttgccga tttcggccta
     9181 ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac
     9241 gtttacaatt tcc
//