LOCUS       pLL3.3;mU6-sgAmpka1-hU6 8252 bp ds-DNA     circular     13-SEP-2021
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    69..249
                     /label="5' LTR (truncated)"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     rep_origin      311..899
                     /label="ori"
                     /ApEinfo_revcolor=#fff79a
                     /ApEinfo_fwdcolor=#fff79a
     misc_feature    complement(1070..1930)
                     /label="AmpR"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    complement(1931..2035)
                     /label="AmpR_Promoter"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    2301..2680
                     /label="CMV_Enhancer"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    2682..2880
                     /label="CMV_Promoter"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     LTR             2898..3078
                     /label="5' LTR (truncated)"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    3125..3250
                     /label="HIV-1 ψ"
                     /ApEinfo_revcolor=#fdc68a
                     /ApEinfo_fwdcolor=#fdc68a
     misc_feature    3278..3592
                     /label="gag (partial)"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    3603..3749
                     /label="env (partial)"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    3689..4043
                     /label="RRE"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    3792..4382
                     /label="gp41"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    4509..4626
                     /label="cPPT/CTS"
                     /ApEinfo_revcolor=#fdc68a
                     /ApEinfo_fwdcolor=#fdc68a
     Promoter        4708..5021
                     /label="mU6"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    5022..5022
                     /label="G+"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_feature    5023..5042
                     /label="sgAmpka1"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    5043..5137
                     /label="cr2"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    5138..5156
                     /label="Gibson 3"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_feature    5185..5433
                     /label="hU6"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    5434..5434
                     /label="G+"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    5435..5454
                     /label="sgAmpka2"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    5455..5549
                     /label="cr3"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     Promoter        5588..6088
                     /label="PGK"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     Gene            6096..7147
                     /label="Cre Recombinase"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     Viral Element   7217..7788
                     /label="WPRE"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
ORIGIN
        1 TAGAGTGGAG GTTTGACAGC CGCCTAGCAT TTCATCACAT GGCCCGAGAG CTGCATCCGG
       61 ACTGTACTGG GTCTCTCTGG TTAGACCAGA TCTGAGCCTG GGAGCTCTCT GGCTAACTAG
      121 GGAACCCACT GCTTAAGCCT CAATAAAGCT TGCCTTGAGT GCTTCAAGTA GTGTGTGCCC
      181 GTCTGTTGTG TGACTCTGGT AACTAGAGAT CCCTCAGACC CTTTTAGTCA GTGTGGAAAA
      241 TCTCTAGCAG CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT
      301 TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA
      361 GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT
      421 CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC
      481 CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG
      541 TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT
      601 TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG
      661 CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA
      721 AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA
      781 AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG
      841 GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG
      901 AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG
      961 GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT
     1021 GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAAACTTG GTCTGACAGT TACCAATGCT
     1081 TAATCAGTGA GGCACCTATC TCAGCGATCT GTCTATTTCG TTCATCCATA GTTGCCTGAC
     1141 TCCCCGTCGT GTAGATAACT ACGATACGGG AGGGCTTACC ATCTGGCCCC AGTGCTGCAA
     1201 TGATACCGCG AGACCCACGC TCACCGGCTC CAGATTTATC AGCAATAAAC CAGCCAGCCG
     1261 GAAGGGCCGA GCGCAGAAGT GGTCCTGCAA CTTTATCCGC CTCCATCCAG TCTATTAATT
     1321 GTTGCCGGGA AGCTAGAGTA AGTAGTTCGC CAGTTAATAG TTTGCGCAAC GTTGTTGCCA
     1381 TTGCTACAGG CATCGTGGTG TCACGCTCGT CGTTTGGTAT GGCTTCATTC AGCTCCGGTT
     1441 CCCAACGATC AAGGCGAGTT ACATGATCCC CCATGTTGTG CAAAAAAGCG GTTAGCTCCT
     1501 TCGGTCCTCC GATCGTTGTC AGAAGTAAGT TGGCCGCAGT GTTATCACTC ATGGTTATGG
     1561 CAGCACTGCA TAATTCTCTT ACTGTCATGC CATCCGTAAG ATGCTTTTCT GTGACTGGTG
     1621 AGTACTCAAC CAAGTCATTC TGAGAATAGT GTATGCGGCG ACCGAGTTGC TCTTGCCCGG
     1681 CGTCAATACG GGATAATACC GCGCCACATA GCAGAACTTT AAAAGTGCTC ATCATTGGAA
     1741 AACGTTCTTC GGGGCGAAAA CTCTCAAGGA TCTTACCGCT GTTGAGATCC AGTTCGATGT
     1801 AACCCACTCG TGCACCCAAC TGATCTTCAG CATCTTTTAC TTTCACCAGC GTTTCTGGGT
     1861 GAGCAAAAAC AGGAAGGCAA AATGCCGCAA AAAAGGGAAT AAGGGCGACA CGGAAATGTT
     1921 GAATACTCAT ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA
     1981 TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT
     2041 TTCCCCGAAA AGTGCCACCT GACGTCGACG GATCGGGAGA TCTCCCGATC CCCTATGGTG
     2101 CACTCTCAGT ACAATCTGCT CTGATGCCGC ATAGTTAAGC CAGTATCTGC TCCCTGCTTG
     2161 TGTGTTGGAG GTCGCTGAGT AGTGCGCGAG CAAAATTTAA GCTACAACAA GGCAAGGCTT
     2221 GACCGACAAT TGCATGAAGA ATCTGCTTAG GGTTAGGCGT TTTGCGCTGC TTCGCGATGT
     2281 ACGGGCCAGA TATACGCGTT GACATTGATT ATTGACTAGT TATTAATAGT AATCAATTAC
     2341 GGGGTCATTA GTTCATAGCC CATATATGGA GTTCCGCGTT ACATAACTTA CGGTAAATGG
     2401 CCCGCCTGGC TGACCGCCCA ACGACCCCCG CCCATTGACG TCAATAATGA CGTATGTTCC
     2461 CATAGTAACG CCAATAGGGA CTTTCCATTG ACGTCAATGG GTGGAGTATT TACGGTAAAC
     2521 TGCCCACTTG GCAGTACATC AAGTGTATCA TATGCCAAGT ACGCCCCCTA TTGACGTCAA
     2581 TGACGGTAAA TGGCCCGCCT GGCATTATGC CCAGTACATG ACCTTATGGG ACTTTCCTAC
     2641 TTGGCAGTAC ATCTACGTAT TAGTCATCGC TATTACCATG GTGATGCGGT TTTGGCAGTA
     2701 CATCAATGGG CGTGGATAGC GGTTTGACTC ACGGGGATTT CCAAGTCTCC ACCCCATTGA
     2761 CGTCAATGGG AGTTTGTTTT GGCACCAAAA TCAACGGGAC TTTCCAAAAT GTCGTAACAA
     2821 CTCCGCCCCA TTGACGCAAA TGGGCGGTAG GCGTGTACGG TGGGAGGTCT ATATAAGCAG
     2881 CGCGTTTTGC CTGTACTGGG TCTCTCTGGT TAGACCAGAT CTGAGCCTGG GAGCTCTCTG
     2941 GCTAACTAGG GAACCCACTG CTTAAGCCTC AATAAAGCTT GCCTTGAGTG CTTCAAGTAG
     3001 TGTGTGCCCG TCTGTTGTGT GACTCTGGTA ACTAGAGATC CCTCAGACCC TTTTAGTCAG
     3061 TGTGGAAAAT CTCTAGCAGT GGCGCCCGAA CAGGGACTTG AAAGCGAAAG GGAAACCAGA
     3121 GGAGCTCTCT CGACGCAGGA CTCGGCTTGC TGAAGCGCGC ACGGCAAGAG GCGAGGGGCG
     3181 GCGACTGGTG AGTACGCCAA AAATTTTGAC TAGCGGAGGC TAGAAGGAGA GAGATGGGTG
     3241 CGAGAGCGTC AGTATTAAGC GGGGGAGAAT TAGATCGCGA TGGGAAAAAA TTCGGTTAAG
     3301 GCCAGGGGGA AAGAAAAAAT ATAAATTAAA ACATATAGTA TGGGCAAGCA GGGAGCTAGA
     3361 ACGATTCGCA GTTAATCCTG GCCTGTTAGA AACATCAGAA GGCTGTAGAC AAATACTGGG
     3421 ACAGCTACAA CCATCCCTTC AGACAGGATC AGAAGAACTT AGATCATTAT ATAATACAGT
     3481 AGCAACCCTC TATTGTGTGC ATCAAAGGAT AGAGATAAAA GACACCAAGG AAGCTTTAGA
     3541 CAAGATAGAG GAAGAGCAAA ACAAAAGTAA GACCACCGCA CAGCAAGCGG CCGGCCGCGC
     3601 TGATCTTCAG ACCTGGAGGA GGAGATATGA GGGACAATTG GAGAAGTGAA TTATATAAAT
     3661 ATAAAGTAGT AAAAATTGAA CCATTAGGAG TAGCACCCAC CAAGGCAAAG AGAAGAGTGG
     3721 TGCAGAGAGA AAAAAGAGCA GTGGGAATAG GAGCTTTGTT CCTTGGGTTC TTGGGAGCAG
     3781 CAGGAAGCAC TATGGGCGCA GCGTCAATGA CGCTGACGGT ACAGGCCAGA CAATTATTGT
     3841 CTGGTATAGT GCAGCAGCAG AACAATTTGC TGAGGGCTAT TGAGGCGCAA CAGCATCTGT
     3901 TGCAACTCAC AGTCTGGGGC ATCAAGCAGC TCCAGGCAAG AATCCTGGCT GTGGAAAGAT
     3961 ACCTAAAGGA TCAACAGCTC CTGGGGATTT GGGGTTGCTC TGGAAAACTC ATTTGCACCA
     4021 CTGCTGTGCC TTGGAATGCT AGTTGGAGTA ATAAATCTCT GGAACAGATT TGGAATCACA
     4081 CGACCTGGAT GGAGTGGGAC AGAGAAATTA ACAATTACAC AAGCTTAATA CACTCCTTAA
     4141 TTGAAGAATC GCAAAACCAG CAAGAAAAGA ATGAACAAGA ATTATTGGAA TTAGATAAAT
     4201 GGGCAAGTTT GTGGAATTGG TTTAACATAA CAAATTGGCT GTGGTATATA AAATTATTCA
     4261 TAATGATAGT AGGAGGCTTG GTAGGTTTAA GAATAGTTTT TGCTGTACTT TCTATAGTGA
     4321 ATAGAGTTAG GCAGGGATAT TCACCATTAT CGTTTCAGAC CCACCTCCCA ACCCCGAGGG
     4381 GACCCGACAG GCCCGAAGGA ATAGAAGAAG AAGGTGGAGA GAGAGACAGA GACAGATCCA
     4441 TTCGATTAGT GAACGGATCG GCACTGCGTG CGCCAATTCT GCAGACAAAT GGCAGTATTC
     4501 ATCCACAATT TTAAAAGAAA AGGGGGGATT GGGGGGTACA GTGCAGGGGA AAGAATAGTA
     4561 GACATAATAG CAACAGACAT ACAAACTAAA GAATTACAAA AACAAATTAC AAAAATTCAA
     4621 AATTTTCGGG TTTATTACAG GGACAGCAGA GATCCAGTTT GGTTAGTACC GGGCCCGCTC
     4681 TAGCCCTGCA GGAACTGTTT AAACTGTgat ccgacgcgcc atctctaggc ccgcgccggc
     4741 cccctcgcac ggacttgtgg gagaagctcg gctactcccc tgccccggtt aatttgcata
     4801 taatatttcc tagtaactat agaggcttaa tgtgcgataa aagacagata atctgttctt
     4861 tttaatacta gctacatttt acatgatagg cttggatttc tataagagat acaaatacta
     4921 aattattatt ttaaaaaaca gcacaaaagg aaactcaccc taactgtaaa gtaattgtgt
     4981 gttttgagac tataagtatc ccttggagaa ccaccttgtt gGCAAGGCTC CGAATCTTCT
     5041 GCgtttgaga gctaagcaga aagctgcata gcaagttcaa ataaggctag tccgtacaca
     5101 acttgaaaaa gtggcagccg agtcggctgc tttttttgct cgaatctaca ctcagctatg
     5161 gcgcgcccca aggtcgggca ggaagagggc ctatttccca tgattccttc atatttgcat
     5221 atacgataca aggctgttag agagataatt ggaattaatt tgactgtaaa cacaaagata
     5281 ttagtacaaa atacgtgacg tagaaagtaa taatttcttg ggtagtttgc agttttaaaa
     5341 ttatgtttta aaatggacta tcatatgctt accgtaactt gaaagtattt cgatttcttg
     5401 gctttatata tcttgtggaa agccagaaac atgGCCAATT GACAGGCCAT AAAGgtttca
     5461 gagctaagca caagagtgca tagcaagttg aaataaggct agtccgttta caacttgaaa
     5521 aagtggcacc cgagtcgggt gctttttttG TTTGGCGCGC CTATGGCTTA ATTAACATGG
     5581 CGGCCGCATT CTACCGGGTA GGGGAGGCGC TTTTCCCAAG GCAGTCTGGA GCATGCGCTT
     5641 TAGCAGCCCC GCTGGGCACT TGGCGCTACA CAAGTGGCCT CTGGCCTCGC ACACATTCCA
     5701 CATCCACCGG TAGGCGCCAA CCGGCTCCGT TCTTTGGTGG CCCCTTCGCG CCACCTTCTA
     5761 CTCCTCCCCT AGTCAGGAAG TTCCCCCCCG CCCCGCAGCT CGCGTCGTGC AGGACGTGAC
     5821 AAATGGAAGT AGCACGTCTC ACTAGTCTCG TGCAGATGGA CAGCACCGCT GAGCAATGGA
     5881 AGCGGGTAGG CCTTTGGGGC AGCGGCCAAT AGCAGCTTTG CTCCTTCGCT TTCTGGGCTC
     5941 AGAGGCTGGG AAGGGGTGGG TCCGGGGGCG GGCTCAGGGG CGGGCTCAGG GGCGGGGCGG
     6001 GCGCCCGAAG GTCCTCCGGA GGCCCGGCAT TCTGCACGCT TCAAAAGCGC ACGTCTGCCG
     6061 CGCTGTTCTC CTCTTCCTCA TCTCCGGGAC CCGGGATGCC CAAGAAGAAG AGGAAGGTGT
     6121 CCAATTTACT GACCGTACAC CAAAATTTGC CTGCATTACC GGTCGATGCA ACGAGTGATG
     6181 AGGTTCGCAA GAACCTGATG GACATGTTCA GGGATCGCCA GGCGTTTTCT GAGCATACCT
     6241 GGAAAATGCT TCTGTCCGTT TGCCGGTCGT GGGCGGCATG GTGCAAGTTG AATAACCGGA
     6301 AATGGTTTCC CGCAGAACCT GAAGATGTTC GCGATTATCT TCTATATCTT CAGGCGCGCG
     6361 GTCTGGCAGT AAAAACTATC CAGCAACATT TGGGCCAGCT AAACATGCTT CATCGTCGGT
     6421 CCGGGCTGCC ACGACCAAGT GACAGCAATG CTGTTTCACT GGTTATGCGG CGGATCCGAA
     6481 AAGAAAACGT TGATGCCGGT GAACGTGCAA AACAGGCTCT AGCGTTCGAA CGCACTGATT
     6541 TCGACCAGGT TCGTTCACTC ATGGAAAATA GCGATCGCTG CCAGGATATA CGTAATCTGG
     6601 CATTTCTGGG GATTGCTTAT AACACCCTGT TACGTATAGC CGAAATTGCC AGGATCAGGG
     6661 TTAAAGATAT CTCACGTACT GACGGTGGGA GAATGTTAAT CCATATTGGC AGAACGAAAA
     6721 CGCTGGTTAG CACCGCAGGT GTAGAGAAGG CACTTAGCCT GGGGGTAACT AAACTGGTCG
     6781 AGCGATGGAT TTCCGTCTCT GGTGTAGCTG ATGATCCGAA TAACTACCTG TTTTGCCGGG
     6841 TCAGAAAAAA TGGTGTTGCC GCGCCATCTG CCACCAGCCA GCTATCAACT CGCGCCCTGG
     6901 AAGGGATTTT TGAAGCAACT CATCGATTGA TTTACGGCGC TAAGGATGAC TCTGGTCAGA
     6961 GATACCTGGC CTGGTCTGGA CACAGTGCCC GTGTCGGAGC CGCGCGAGAT ATGGCCCGCG
     7021 CTGGAGTTTC AATACCGGAG ATCATGCAAG CTGGTGGCTG GACCAATGTA AATATTGTCA
     7081 TGAACTATAT CCGTAACCTG GATAGTGAAA CAGGGGCAAT GGTGCGCCTG CTGGAAGATG
     7141 GCGATTAGCC ATGTTTAAGG GTTCCGGTTC CACTAGGTAC AATTCGATAT CAAGCTTATC
     7201 GATAATCAAC CTCTGGATTA CAAAATTTGT GAAAGATTGA CTGGTATTCT TAACTATGTT
     7261 GCTCCTTTTA CGCTATGTGG ATACGCTGCT TTAATGCCTT TGTATCATGC TATTGCTTCC
     7321 CGTATGGCTT TCATTTTCTC CTCCTTGTAT AAATCCTGGT TGCTGTCTCT TTATGAGGAG
     7381 TTGTGGCCCG TTGTCAGGCA ACGTGGCGTG GTGTGCACTG TGTTTGCTGA CGCAACCCCC
     7441 ACTGGTTGGG GCATTGCCAC CACCTGTCAG CTCCTTTCCG GGACTTTCGC TTTCCCCCTC
     7501 CCTATTGCCA CGGCGGAACT CATCGCCGCC TGCCTTGCCC GCTGCTGGAC AGGGGCTCGG
     7561 CTGTTGGGCA CTGACAATTC CGTGGTGTTG TCGGGGAAAT CATCGTCCTT TCCTTGGCTG
     7621 CTCGCCTGTG TTGCCACCTG GATTCTGCGC GGGACGTCCT TCTGCTACGT CCCTTCGGCC
     7681 CTCAATCCAG CGGACCTTCC TTCCCGCGGC CTGCTGCCGG CTCTGCGGCC TCTTCCGCGT
     7741 CTTCGCCTTC GCCCTCAGAC GAGTCGGATC TCCCTTTGGG CCGCCTCCCC GCATCGATAC
     7801 CGTCGACCTC GATCGAGACC TAGAAAAACA TGGAGCAATC ACAAGTAGCA ATACAGCAGC
     7861 TACCAATGCT GATTGTGCCT GGCTAGAAGC ACAAGAGGAG GAGGAGGTGG GTTTTCCAGT
     7921 CACACCTCAG GTACCTTTAA GACCAATGAC TTACAAGGCA GCTGTAGATC TTAGCCACTT
     7981 TTTAAAAGAA AAGGGGGGAC TGGAAGGGCT AATTCACTCC CAACGAAGAC AAGATATCCT
     8041 TGATCTGTGG ATCTACCACA CACAAGGCTA CTTCCCTGAT TGGCAGAACT ACACACCAGG
     8101 GCCAGGGATC AGATATCCAC TGACCTTTGG ATGGTGCTAC AAGCTAGTAC CAGTTGAGCA
     8161 AGAGAAGGTA GAAGAAGCCA ATGAAGGAGA GAACACCCGC TTGTTACACC CTGTGAGCCT
     8221 GCATGGGATG GATGACCCGG AGAGAGAAGT AT
//