LOCUS       Copy_of_pLL3.3;mU6-sgCe 8251 bp ds-DNA     circular     13-SEP-2021
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    69..249
                     /label="5' LTR (truncated)"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     rep_origin      311..899
                     /label="ori"
                     /ApEinfo_revcolor=#fff79a
                     /ApEinfo_fwdcolor=#fff79a
     misc_feature    complement(1070..1930)
                     /label="AmpR"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    complement(1931..2035)
                     /label="AmpR_Promoter"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    2301..2680
                     /label="CMV_Enhancer"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    2682..2880
                     /label="CMV_Promoter"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     LTR             2898..3078
                     /label="5' LTR (truncated)"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    3125..3250
                     /label="HIV-1 ψ"
                     /ApEinfo_revcolor=#fdc68a
                     /ApEinfo_fwdcolor=#fdc68a
     misc_feature    3278..3592
                     /label="gag (partial)"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    3603..3749
                     /label="env (partial)"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    3689..4043
                     /label="RRE"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    3792..4382
                     /label="gp41"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    4509..4626
                     /label="cPPT/CTS"
                     /ApEinfo_revcolor=#fdc68a
                     /ApEinfo_fwdcolor=#fdc68a
     Promoter        4708..5021
                     /label="mU6"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    5022..5042
                     /label="sgCebpa_v2"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    5043..5137
                     /label="cr2"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    5138..5156
                     /label="Gibson 3"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_feature    5185..5433
                     /label="hU6"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    5434..5453
                     /label="sgCebpb_v2"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    5454..5548
                     /label="cr3"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     Promoter        5587..6087
                     /label="PGK"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     Gene            6095..7146
                     /label="Cre Recombinase"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     Viral Element   7216..7787
                     /label="WPRE"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
ORIGIN
        1 TAGAGTGGAG GTTTGACAGC CGCCTAGCAT TTCATCACAT GGCCCGAGAG CTGCATCCGG
       61 ACTGTACTGG GTCTCTCTGG TTAGACCAGA TCTGAGCCTG GGAGCTCTCT GGCTAACTAG
      121 GGAACCCACT GCTTAAGCCT CAATAAAGCT TGCCTTGAGT GCTTCAAGTA GTGTGTGCCC
      181 GTCTGTTGTG TGACTCTGGT AACTAGAGAT CCCTCAGACC CTTTTAGTCA GTGTGGAAAA
      241 TCTCTAGCAG CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT
      301 TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA
      361 GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT
      421 CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC
      481 CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG
      541 TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT
      601 TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG
      661 CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA
      721 AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA
      781 AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG
      841 GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG
      901 AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG
      961 GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT
     1021 GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAAACTTG GTCTGACAGT TACCAATGCT
     1081 TAATCAGTGA GGCACCTATC TCAGCGATCT GTCTATTTCG TTCATCCATA GTTGCCTGAC
     1141 TCCCCGTCGT GTAGATAACT ACGATACGGG AGGGCTTACC ATCTGGCCCC AGTGCTGCAA
     1201 TGATACCGCG AGACCCACGC TCACCGGCTC CAGATTTATC AGCAATAAAC CAGCCAGCCG
     1261 GAAGGGCCGA GCGCAGAAGT GGTCCTGCAA CTTTATCCGC CTCCATCCAG TCTATTAATT
     1321 GTTGCCGGGA AGCTAGAGTA AGTAGTTCGC CAGTTAATAG TTTGCGCAAC GTTGTTGCCA
     1381 TTGCTACAGG CATCGTGGTG TCACGCTCGT CGTTTGGTAT GGCTTCATTC AGCTCCGGTT
     1441 CCCAACGATC AAGGCGAGTT ACATGATCCC CCATGTTGTG CAAAAAAGCG GTTAGCTCCT
     1501 TCGGTCCTCC GATCGTTGTC AGAAGTAAGT TGGCCGCAGT GTTATCACTC ATGGTTATGG
     1561 CAGCACTGCA TAATTCTCTT ACTGTCATGC CATCCGTAAG ATGCTTTTCT GTGACTGGTG
     1621 AGTACTCAAC CAAGTCATTC TGAGAATAGT GTATGCGGCG ACCGAGTTGC TCTTGCCCGG
     1681 CGTCAATACG GGATAATACC GCGCCACATA GCAGAACTTT AAAAGTGCTC ATCATTGGAA
     1741 AACGTTCTTC GGGGCGAAAA CTCTCAAGGA TCTTACCGCT GTTGAGATCC AGTTCGATGT
     1801 AACCCACTCG TGCACCCAAC TGATCTTCAG CATCTTTTAC TTTCACCAGC GTTTCTGGGT
     1861 GAGCAAAAAC AGGAAGGCAA AATGCCGCAA AAAAGGGAAT AAGGGCGACA CGGAAATGTT
     1921 GAATACTCAT ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA
     1981 TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT
     2041 TTCCCCGAAA AGTGCCACCT GACGTCGACG GATCGGGAGA TCTCCCGATC CCCTATGGTG
     2101 CACTCTCAGT ACAATCTGCT CTGATGCCGC ATAGTTAAGC CAGTATCTGC TCCCTGCTTG
     2161 TGTGTTGGAG GTCGCTGAGT AGTGCGCGAG CAAAATTTAA GCTACAACAA GGCAAGGCTT
     2221 GACCGACAAT TGCATGAAGA ATCTGCTTAG GGTTAGGCGT TTTGCGCTGC TTCGCGATGT
     2281 ACGGGCCAGA TATACGCGTT GACATTGATT ATTGACTAGT TATTAATAGT AATCAATTAC
     2341 GGGGTCATTA GTTCATAGCC CATATATGGA GTTCCGCGTT ACATAACTTA CGGTAAATGG
     2401 CCCGCCTGGC TGACCGCCCA ACGACCCCCG CCCATTGACG TCAATAATGA CGTATGTTCC
     2461 CATAGTAACG CCAATAGGGA CTTTCCATTG ACGTCAATGG GTGGAGTATT TACGGTAAAC
     2521 TGCCCACTTG GCAGTACATC AAGTGTATCA TATGCCAAGT ACGCCCCCTA TTGACGTCAA
     2581 TGACGGTAAA TGGCCCGCCT GGCATTATGC CCAGTACATG ACCTTATGGG ACTTTCCTAC
     2641 TTGGCAGTAC ATCTACGTAT TAGTCATCGC TATTACCATG GTGATGCGGT TTTGGCAGTA
     2701 CATCAATGGG CGTGGATAGC GGTTTGACTC ACGGGGATTT CCAAGTCTCC ACCCCATTGA
     2761 CGTCAATGGG AGTTTGTTTT GGCACCAAAA TCAACGGGAC TTTCCAAAAT GTCGTAACAA
     2821 CTCCGCCCCA TTGACGCAAA TGGGCGGTAG GCGTGTACGG TGGGAGGTCT ATATAAGCAG
     2881 CGCGTTTTGC CTGTACTGGG TCTCTCTGGT TAGACCAGAT CTGAGCCTGG GAGCTCTCTG
     2941 GCTAACTAGG GAACCCACTG CTTAAGCCTC AATAAAGCTT GCCTTGAGTG CTTCAAGTAG
     3001 TGTGTGCCCG TCTGTTGTGT GACTCTGGTA ACTAGAGATC CCTCAGACCC TTTTAGTCAG
     3061 TGTGGAAAAT CTCTAGCAGT GGCGCCCGAA CAGGGACTTG AAAGCGAAAG GGAAACCAGA
     3121 GGAGCTCTCT CGACGCAGGA CTCGGCTTGC TGAAGCGCGC ACGGCAAGAG GCGAGGGGCG
     3181 GCGACTGGTG AGTACGCCAA AAATTTTGAC TAGCGGAGGC TAGAAGGAGA GAGATGGGTG
     3241 CGAGAGCGTC AGTATTAAGC GGGGGAGAAT TAGATCGCGA TGGGAAAAAA TTCGGTTAAG
     3301 GCCAGGGGGA AAGAAAAAAT ATAAATTAAA ACATATAGTA TGGGCAAGCA GGGAGCTAGA
     3361 ACGATTCGCA GTTAATCCTG GCCTGTTAGA AACATCAGAA GGCTGTAGAC AAATACTGGG
     3421 ACAGCTACAA CCATCCCTTC AGACAGGATC AGAAGAACTT AGATCATTAT ATAATACAGT
     3481 AGCAACCCTC TATTGTGTGC ATCAAAGGAT AGAGATAAAA GACACCAAGG AAGCTTTAGA
     3541 CAAGATAGAG GAAGAGCAAA ACAAAAGTAA GACCACCGCA CAGCAAGCGG CCGGCCGCGC
     3601 TGATCTTCAG ACCTGGAGGA GGAGATATGA GGGACAATTG GAGAAGTGAA TTATATAAAT
     3661 ATAAAGTAGT AAAAATTGAA CCATTAGGAG TAGCACCCAC CAAGGCAAAG AGAAGAGTGG
     3721 TGCAGAGAGA AAAAAGAGCA GTGGGAATAG GAGCTTTGTT CCTTGGGTTC TTGGGAGCAG
     3781 CAGGAAGCAC TATGGGCGCA GCGTCAATGA CGCTGACGGT ACAGGCCAGA CAATTATTGT
     3841 CTGGTATAGT GCAGCAGCAG AACAATTTGC TGAGGGCTAT TGAGGCGCAA CAGCATCTGT
     3901 TGCAACTCAC AGTCTGGGGC ATCAAGCAGC TCCAGGCAAG AATCCTGGCT GTGGAAAGAT
     3961 ACCTAAAGGA TCAACAGCTC CTGGGGATTT GGGGTTGCTC TGGAAAACTC ATTTGCACCA
     4021 CTGCTGTGCC TTGGAATGCT AGTTGGAGTA ATAAATCTCT GGAACAGATT TGGAATCACA
     4081 CGACCTGGAT GGAGTGGGAC AGAGAAATTA ACAATTACAC AAGCTTAATA CACTCCTTAA
     4141 TTGAAGAATC GCAAAACCAG CAAGAAAAGA ATGAACAAGA ATTATTGGAA TTAGATAAAT
     4201 GGGCAAGTTT GTGGAATTGG TTTAACATAA CAAATTGGCT GTGGTATATA AAATTATTCA
     4261 TAATGATAGT AGGAGGCTTG GTAGGTTTAA GAATAGTTTT TGCTGTACTT TCTATAGTGA
     4321 ATAGAGTTAG GCAGGGATAT TCACCATTAT CGTTTCAGAC CCACCTCCCA ACCCCGAGGG
     4381 GACCCGACAG GCCCGAAGGA ATAGAAGAAG AAGGTGGAGA GAGAGACAGA GACAGATCCA
     4441 TTCGATTAGT GAACGGATCG GCACTGCGTG CGCCAATTCT GCAGACAAAT GGCAGTATTC
     4501 ATCCACAATT TTAAAAGAAA AGGGGGGATT GGGGGGTACA GTGCAGGGGA AAGAATAGTA
     4561 GACATAATAG CAACAGACAT ACAAACTAAA GAATTACAAA AACAAATTAC AAAAATTCAA
     4621 AATTTTCGGG TTTATTACAG GGACAGCAGA GATCCAGTTT GGTTAGTACC GGGCCCGCTC
     4681 TAGCCCTGCA GGAACTGTTT AAACTGTgat ccgacgcgcc atctctaggc ccgcgccggc
     4741 cccctcgcac ggacttgtgg gagaagctcg gctactcccc tgccccggtt aatttgcata
     4801 taatatttcc tagtaactat agaggcttaa tgtgcgataa aagacagata atctgttctt
     4861 tttaatacta gctacatttt acatgatagg cttggatttc tataagagat acaaatacta
     4921 aattattatt ttaaaaaaca gcacaaaagg aaactcaccc taactgtaaa gtaattgtgt
     4981 gttttgagac tataagtatc ccttggagaa ccaccttgtt gGCATCAGCG CCTACATCGA
     5041 CCgtttgaga gctaagcaga aagctgcata gcaagttcaa ataaggctag tccgtacaca
     5101 acttgaaaaa gtggcagccg agtcggctgc tttttttgct cgaatctaca ctcagctatg
     5161 gcgcgcccca aggtcgggca ggaagagggc ctatttccca tgattccttc atatttgcat
     5221 atacgataca aggctgttag agagataatt ggaattaatt tgactgtaaa cacaaagata
     5281 ttagtacaaa atacgtgacg tagaaagtaa taatttcttg ggtagtttgc agttttaaaa
     5341 ttatgtttta aaatggacta tcatatgctt accgtaactt gaaagtattt cgatttcttg
     5401 gctttatata tcttgtggaa agccagaaac atgGTGCTCG CCAATGGCCG GCTgtttcag
     5461 agctaagcac aagagtgcat agcaagttga aataaggcta gtccgtttac aacttgaaaa
     5521 agtggcaccc gagtcgggtg ctttttttGT TTGGCGCGCC TATGGCTTAA TTAACATGGC
     5581 GGCCGCATTC TACCGGGTAG GGGAGGCGCT TTTCCCAAGG CAGTCTGGAG CATGCGCTTT
     5641 AGCAGCCCCG CTGGGCACTT GGCGCTACAC AAGTGGCCTC TGGCCTCGCA CACATTCCAC
     5701 ATCCACCGGT AGGCGCCAAC CGGCTCCGTT CTTTGGTGGC CCCTTCGCGC CACCTTCTAC
     5761 TCCTCCCCTA GTCAGGAAGT TCCCCCCCGC CCCGCAGCTC GCGTCGTGCA GGACGTGACA
     5821 AATGGAAGTA GCACGTCTCA CTAGTCTCGT GCAGATGGAC AGCACCGCTG AGCAATGGAA
     5881 GCGGGTAGGC CTTTGGGGCA GCGGCCAATA GCAGCTTTGC TCCTTCGCTT TCTGGGCTCA
     5941 GAGGCTGGGA AGGGGTGGGT CCGGGGGCGG GCTCAGGGGC GGGCTCAGGG GCGGGGCGGG
     6001 CGCCCGAAGG TCCTCCGGAG GCCCGGCATT CTGCACGCTT CAAAAGCGCA CGTCTGCCGC
     6061 GCTGTTCTCC TCTTCCTCAT CTCCGGGACC CGGGATGCCC AAGAAGAAGA GGAAGGTGTC
     6121 CAATTTACTG ACCGTACACC AAAATTTGCC TGCATTACCG GTCGATGCAA CGAGTGATGA
     6181 GGTTCGCAAG AACCTGATGG ACATGTTCAG GGATCGCCAG GCGTTTTCTG AGCATACCTG
     6241 GAAAATGCTT CTGTCCGTTT GCCGGTCGTG GGCGGCATGG TGCAAGTTGA ATAACCGGAA
     6301 ATGGTTTCCC GCAGAACCTG AAGATGTTCG CGATTATCTT CTATATCTTC AGGCGCGCGG
     6361 TCTGGCAGTA AAAACTATCC AGCAACATTT GGGCCAGCTA AACATGCTTC ATCGTCGGTC
     6421 CGGGCTGCCA CGACCAAGTG ACAGCAATGC TGTTTCACTG GTTATGCGGC GGATCCGAAA
     6481 AGAAAACGTT GATGCCGGTG AACGTGCAAA ACAGGCTCTA GCGTTCGAAC GCACTGATTT
     6541 CGACCAGGTT CGTTCACTCA TGGAAAATAG CGATCGCTGC CAGGATATAC GTAATCTGGC
     6601 ATTTCTGGGG ATTGCTTATA ACACCCTGTT ACGTATAGCC GAAATTGCCA GGATCAGGGT
     6661 TAAAGATATC TCACGTACTG ACGGTGGGAG AATGTTAATC CATATTGGCA GAACGAAAAC
     6721 GCTGGTTAGC ACCGCAGGTG TAGAGAAGGC ACTTAGCCTG GGGGTAACTA AACTGGTCGA
     6781 GCGATGGATT TCCGTCTCTG GTGTAGCTGA TGATCCGAAT AACTACCTGT TTTGCCGGGT
     6841 CAGAAAAAAT GGTGTTGCCG CGCCATCTGC CACCAGCCAG CTATCAACTC GCGCCCTGGA
     6901 AGGGATTTTT GAAGCAACTC ATCGATTGAT TTACGGCGCT AAGGATGACT CTGGTCAGAG
     6961 ATACCTGGCC TGGTCTGGAC ACAGTGCCCG TGTCGGAGCC GCGCGAGATA TGGCCCGCGC
     7021 TGGAGTTTCA ATACCGGAGA TCATGCAAGC TGGTGGCTGG ACCAATGTAA ATATTGTCAT
     7081 GAACTATATC CGTAACCTGG ATAGTGAAAC AGGGGCAATG GTGCGCCTGC TGGAAGATGG
     7141 CGATTAGCCA TGTTTAAGGG TTCCGGTTCC ACTAGGTACA ATTCGATATC AAGCTTATCG
     7201 ATAATCAACC TCTGGATTAC AAAATTTGTG AAAGATTGAC TGGTATTCTT AACTATGTTG
     7261 CTCCTTTTAC GCTATGTGGA TACGCTGCTT TAATGCCTTT GTATCATGCT ATTGCTTCCC
     7321 GTATGGCTTT CATTTTCTCC TCCTTGTATA AATCCTGGTT GCTGTCTCTT TATGAGGAGT
     7381 TGTGGCCCGT TGTCAGGCAA CGTGGCGTGG TGTGCACTGT GTTTGCTGAC GCAACCCCCA
     7441 CTGGTTGGGG CATTGCCACC ACCTGTCAGC TCCTTTCCGG GACTTTCGCT TTCCCCCTCC
     7501 CTATTGCCAC GGCGGAACTC ATCGCCGCCT GCCTTGCCCG CTGCTGGACA GGGGCTCGGC
     7561 TGTTGGGCAC TGACAATTCC GTGGTGTTGT CGGGGAAATC ATCGTCCTTT CCTTGGCTGC
     7621 TCGCCTGTGT TGCCACCTGG ATTCTGCGCG GGACGTCCTT CTGCTACGTC CCTTCGGCCC
     7681 TCAATCCAGC GGACCTTCCT TCCCGCGGCC TGCTGCCGGC TCTGCGGCCT CTTCCGCGTC
     7741 TTCGCCTTCG CCCTCAGACG AGTCGGATCT CCCTTTGGGC CGCCTCCCCG CATCGATACC
     7801 GTCGACCTCG ATCGAGACCT AGAAAAACAT GGAGCAATCA CAAGTAGCAA TACAGCAGCT
     7861 ACCAATGCTG ATTGTGCCTG GCTAGAAGCA CAAGAGGAGG AGGAGGTGGG TTTTCCAGTC
     7921 ACACCTCAGG TACCTTTAAG ACCAATGACT TACAAGGCAG CTGTAGATCT TAGCCACTTT
     7981 TTAAAAGAAA AGGGGGGACT GGAAGGGCTA ATTCACTCCC AACGAAGACA AGATATCCTT
     8041 GATCTGTGGA TCTACCACAC ACAAGGCTAC TTCCCTGATT GGCAGAACTA CACACCAGGG
     8101 CCAGGGATCA GATATCCACT GACCTTTGGA TGGTGCTACA AGCTAGTACC AGTTGAGCAA
     8161 GAGAAGGTAG AAGAAGCCAA TGAAGGAGAG AACACCCGCT TGTTACACCC TGTGAGCCTG
     8221 CATGGGATGG ATGACCCGGA GAGAGAAGTA T
//